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Gene information
General information
TC014886 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9104770..9119360
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014886
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen posterior partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen posterior circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: There is a small number of some other defects such as whole larvae inside-out, cuticle crumbs, thorax dorsal not closed.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    head size decreased - Number of animals showing the phenotype: 5

    head slightly orientation irregular - Number of animals showing the phenotype: 5
    wings shortened - Number of animals showing the phenotype: 5

    wings orientation irregular - Number of animals showing the phenotype: 5
    pronotum slightly anteroposterior-axis shortened - Number of animals showing the phenotype: 5
    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 4

    elytra surface irregular - Number of animals showing the phenotype: 4


    elytra dorsal not closed - Number of animals showing the phenotype: 4

    abdomen posterior orientation irregular - Number of animals showing the phenotype: 3

    Comment: posterior right side twisted dorsally

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014886 RA
      MTESTPICR CRVLYLGSS VPQQTKDGL QGIQEPLQE 
      LYPDQGATG ARGIDSWLS VWSNGILLE NVDENHKKI 
      TRFFPIESL HYCAAVRYV LVPEKNTLH SPSPRFLPL 
      DSPFARAPN PTHPPLFAA ILRRTTGIK VLECHAFIC 
      KREVAANAL VRCCFHAYA DSSYAKQVD TAGSIYGTL 
      ASDRLSNKD KVEEWKMTR STSGSTMTI NTIGNGKDD 
      VSIYNGDEN HKVWAGSQD NLDTIYDVY ASSTISRPS 
      RPRQITQPV AVPPPPIKE KKKKSKKSL SREDLYPPL 
      TNGKANSVA GTMIKPRPQ PPPQPVYIV AHPQTLPNP 
      KFFKHHGNT FSHRPRGKM LIPARPIPP PLVPVAPVI 
      IPTTIPKKQ KKAKHAMEE PIYMPSNRA LSPVASYQP 
      VNFPHEAYL MQHYATMES QGKQKRRDK GKLNKKLAQ 
      SMNGLDDPN LMMGGTGPP PGAAAGDES PFNTGIYRK 
      KGHLNERAF SYSIRQEHR SRSYGSLAN LKFATPIPN 
      GDVEDREDM KKEREIMQM VQDLDLSGD ELERSEVPR 
      TMYEARGGP PGPVIVAAP QMNGRGHRR
    CDS sequence
    • >TC014886 RA
      atgacggaa tcgactccg atctgtcga tgccgggtc 
      ctctacttg ggcagttcg gtcccccaa cagaccaaa 
      gacggtctc cagggcatc caagagccc cttcaggag 
      ctctacccc gaccagggg gccaccggg gctcgaggc 
      atcgacagc tggttgagc gtctggtcc aacggcatt 
      ctcctcgag aacgtcgac gaaaatcac aagaaaatc 
      acacgattc ttccccatc gaaagtctc cactattgt 
      gcagctgta agatatgtt ctagtccct gagaaaaac 
      acgctgcac agtcccagc cctcggttc ctccctctc 
      gactcgcca ttcgcccgt gcccctaac cccacccac 
      ccccctctc ttcgccgcc atattacga agaaccact 
      ggaatcaaa gtacttgaa tgccacgcc tttatctgc 
      aaacgcgaa gtggcagcc aatgcgctc gtccgttgc 
      tgtttccac gcctacgct gactcctcc tacgccaag 
      caagttgac acggctggt agtatatac ggtactcta 
      gcctcagac cgactgtcc aacaaagac aaagtagag 
      gaatggaag atgacccgc tcgacatcc gggtcaacc 
      atgaccatc aacaccatc ggtaacggc aaagatgac 
      gtcagtatt tacaacggt gacgaaaac cacaaagtg 
      tgggcgggg tctcaggac aaccttgac acaatctac 
      gacgtttac gcctcctcg accatctcc cgcccctca 
      cgcccccgc cagatcacc caaccggtg gccgtcccg 
      cccccgccc atcaaagag aagaagaaa aaatcgaaa 
      aagtccctc agtcgtgaa gacctctac ccgcctctc 
      accaacggc aaggccaat agcgtggcc ggcacgatg 
      atcaagcca cgcccacaa ccaccgccc caaccagtt 
      tacatcgtc gcccatcct caaaccctc cccaatccg 
      aaattcttc aaacaccat ggcaacaca ttctcgcac 
      cgcccacga ggcaaaatg ctcatcccc gcccggccc 
      atcccccca ccccttgtt cccgtcgcc ccggtcatt 
      atccccacc actattcct aaaaaacaa aaaaaggcg 
      aaacatgcc atggaagaa ccgatttat atgccgagt 
      aaccgggcg ttatcaccg gttgcaagt taccaaccg 
      gttaatttc ccacacgag gcctacttg atgcagcat 
      tacgccacg atggagtcg caggggaag caaaagagg 
      agggacaag ggcaagttg aataaaaaa ttagcgcaa 
      agtatgaac ggattggat gatccgaac ttaatgatg 
      ggcgggacg ggaccgccc ccaggggcg gccgcaggc 
      gatgagagt ccgttcaat acggggatt tacaggaaa 
      aagggacat ttgaatgag agggcgttc tcgtattca 
      atcaggcag gagcacagg agtcgcagc tacggctca 
      ttggccaat ctcaagttc gccacgccc attcccaac 
      ggagatgtg gaagaccgc gaagacatg aagaaagag 
      cgcgagatc atgcagatg gtgcaggac ttggacctc 
      tctggcgac gagttggag cgctcggag gtgccccgt 
      accatgtac gaagcccga gggggcccc ccggggccg 
      gtgattgtg gcggcgccc caaatgaat ggtcgcggc 
      caccgcaga tag
    mRNA sequence
    • >TC014886 RA
      aatcacatt cctgtccta gccgactac gtggatacg 
      cgatcgttg aaatcaccg acgcaacag ccacttgat 
      tgtgaaaaa aacccatcg aaatcaacc cctcggtgt 
      gacaagtga atgcgacaa gtgaaaaac aacacgccg 
      acttcgata cgggcgcga aaaacactg atggtgtgt 
      ggcgttgtg gaattaagg tgtggaaaa gccatttat 
      tgaatgatc ggaccgaga gggccctcc gaaatgacg 
      gaatcgact ccgatctgt cgatgccgg gtcctctac 
      ttgggcagt tcggtcccc caacagacc aaagacggt 
      ctccagggc atccaagag ccccttcag gagctctac 
      cccgaccag ggggccacc ggggctcga ggcatcgac 
      agctggttg agcgtctgg tccaacggc attctcctc 
      gagaacgtc gacgaaaat cacaagaaa atcacacga 
      ttcttcccc atcgaaagt ctccactat tgtgcagct 
      gtaagatat gttctagtc cctgagaaa aacacgctg 
      cacagtccc agccctcgg ttcctccct ctcgactcg 
      ccattcgcc cgtgcccct aaccccacc cacccccct 
      ctcttcgcc gccatatta cgaagaacc actggaatc 
      aaagtactt gaatgccac gcctttatc tgcaaacgc 
      gaagtggca gccaatgcg ctcgtccgt tgctgtttc 
      cacgcctac gctgactcc tcctacgcc aagcaagtt 
      gacacggct ggtagtata tacggtact ctagcctca 
      gaccgactg tccaacaaa gacaaagta gaggaatgg 
      aagatgacc cgctcgaca tccgggtca accatgacc 
      atcaacacc atcggtaac ggcaaagat gacgtcagt 
      atttacaac ggtgacgaa aaccacaaa gtgtgggcg 
      gggtctcag gacaacctt gacacaatc tacgacgtt 
      tacgcctcc tcgaccatc tcccgcccc tcacgcccc 
      cgccagatc acccaaccg gtggccgtc ccgcccccg 
      cccatcaaa gagaagaag aaaaaatcg aaaaagtcc 
      ctcagtcgt gaagacctc tacccgcct ctcaccaac 
      ggcaaggcc aatagcgtg gccggcacg atgatcaag 
      ccacgccca caaccaccg ccccaacca gtttacatc 
      gtcgcccat cctcaaacc ctccccaat ccgaaattc 
      ttcaaacac catggcaac acattctcg caccgccca 
      cgaggcaaa atgctcatc cccgcccgg cccatcccc 
      ccacccctt gttcccgtc gccccggtc attatcccc 
      accactatt cctaaaaaa caaaaaaag gcgaaacat 
      gccatggaa gaaccgatt tatatgccg agtaaccgg 
      gcgttatca ccggttgca agttaccaa ccggttaat 
      ttcccacac gaggcctac ttgatgcag cattacgcc 
      acgatggag tcgcagggg aagcaaaag aggagggac 
      aagggcaag ttgaataaa aaattagcg caaagtatg 
      aacggattg gatgatccg aacttaatg atgggcggg 
      acgggaccg cccccaggg gcggccgca ggcgatgag 
      agtccgttc aatacgggg atttacagg aaaaaggga 
      catttgaat gagagggcg ttctcgtat tcaatcagg 
      caggagcac aggagtcgc agctacggc tcattggcc 
      aatctcaag ttcgccacg cccattccc aacggagat 
      gtggaagac cgcgaagac atgaagaaa gagcgcgag 
      atcatgcag atggtgcag gacttggac ctctctggc 
      gacgagttg gagcgctcg gaggtgccc cgtaccatg 
      tacgaagcc cgagggggc cccccgggg ccggtgatt 
      gtggcggcg ccccaaatg aatggtcgc ggccaccgc 
      agataggtt tgctcaatt gtttaaata attctctat 
      ttaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY