Status
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Gene information
General information
TC033468
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014873,TC014876,TC014874,TC014875
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9318744..9335573
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033468
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05713

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head complete not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033468 RA
      MDPLSGSCY NLNSRRLSS QILAQQKID QKYSQISGS 
      VITLSKYEQ RNFKNLTPQ LLAAVLVTS YHVSIGISL 
      AFSAIVLPQ LKSHPEFKV SECEASWIA SIVALSTPL 
      GSLIVGFLM DQYGRLKTL AMASIPAIS GWVLIALSD 
      NVLLLITGR ALVGFASSI GSSPAVVYL TEIARKDMR 
      GSLICFAQA LTSLGMVLA FIMGYFLDW KQVAWFTNI 
      FIVIPCILV FFIPESPAW LVSKNRIEE AKKSLLWIN 
      KYQTVQLSL VQLSLLQRE HELKESETS KMDTIKELG 
      KPTGYKPLL ILTGLFLFQ QFSGIFTFL FYSITFFQE 
      VGSTMNPYL TSIFIGIVR FVMCMVNTY VLRTYGRRP 
      LVILSCFGM SVSIFLSGF FTHWVKTGV TTLTWLPVL 
      FLLLFVFTS MIGLVPIPY TMTAELFPL EIRGVAHSI 
      STCLASIFT FASLQLYPV MYQGFGGIH GVQYFFAGV 
      TLIAAFYVY VFLPETHQK KLSEIEDYF NKPPKAEKQ 
      QKQIVQEVN ETSKMDTIK ELGKPTGYK PLLILTGLF 
      LFQQFSGIF TFLFYSITF FQEVGSTMN PYLTSIFIG 
      IVRFVMCMV NTYVLRTYG RRPLVILSC FGMSVSIFL 
      SGFFTHWVK TGVTTLTWL PVLFLLLFV FTSMIGLVP 
      IPYTMTAEL FPLEIRGVA HSISTCLAS IFTFASLQL 
      YPVMYQGFG GIHGVQYFF AGVTLLAAV YVYVFLPET 
      HQKKLSEIE DYFNKPTKG IDSNLAAAV YLTGIDVIF 
      FAQALTSLG MVLAFIMGY FLDWKQVAC FTNIFIVIP 
      CILVFFIPE SPAWLVSKN RIEEAKKSL LWINKYQTV 
      QLSLVQLSL LQREHELKE SETSKMDTI KELGKPTGY 
      KPLLILTGL FLFQQFSGI FTFLFYSIT FFQEVGSTM 
      NPYLTSIFI GIVRFVMCM VNTYVLRTY GRRPLVILS 
      CFGMSVSIF LSGFFTHWV KTGVTTLTW LPVLFLLLF 
      VFTSMIGLV PIPYTMTAE LFPLEIRGV AHSISTCLA 
      SIFTFASLQ LYPVMYQGF GGIHGVQYF FAGVTLLAA 
      VYVYVFLPE THQKKLSEI EDYFNKPPK AEKQQKQIV 
      QEVLIYFIL LLYPVIKQL GKPTGYKPL LILTGLFLF 
      QQFSGIFTF LFYSITFFQ EVGSTMNPY LTSIFIGIV 
      RFVMCMVNT YVLRTYGRR PLVILSCFG MSVSIFLSG 
      FFTHWVKTG VTTLTWLPV LFLLLFVFT SMIGLVPIP 
      YTMTAELFP LEIRGVAHS ISTCLASIF TFASLQLYP 
      VMYQGFGGI HGVQYFFAG VTLIAAVYV YVFLPETHQ 
      KKLSEIEDY FNKPPKGEK QQKQIVQEV
    CDS sequence
    • >TC033468 RA
      atggaccct cttagtgga agttgctac aatctcaac 
      tcacgaaga ctctcaagc cagatcctc gcccaacaa 
      aaaatcgac caaaaatac tcacaaatt tcaggcagc 
      gttatcaca ctcagcaaa tatgaacaa cgcaacttc 
      aagaatctc acaccacaa ttactagcc gctgtttta 
      gtcacttcc taccatgtc tcgattgga atctcgttg 
      gcattctcc gccatagtc ctcccccag ctcaaatct 
      caccccgaa tttaaagtc agcgaatgc gaggcgtca 
      tggattgca agcattgtc gccttgagc acccccctg 
      ggaagcctc atcgtgggc ttcctcatg gaccaatac 
      ggccgcctc aagaccctc gccatggcc tccattcct 
      gcgatcagt ggctgggtc ctgatcgct ctttctgac 
      aacgtcctt ttactgata acaggacgc gcgttggtt 
      ggtttcgcc tcaagcatc ggctcgagt cctgcggtg 
      gtttatttg accgaaatc gcccgaaag gacatgcga 
      ggatcgttg atttgtttc gcccaagct ttaacctcc 
      ctcgggatg gtgctagcc ttcatcatg ggctatttc 
      ctggactgg aaacaagtc gcctggttc acgaacatc 
      ttcatcgta atcccgtgc atcctcgtg ttcttcatc 
      ccggaaagt cccgcctgg ctcgtgtcg aaaaaccgg 
      atagaagaa gcgaaaaaa tcactgctt tggatcaac 
      aaataccaa acggtgcag ttgtcactc gttcagctc 
      tcgcttctc caaagggaa cacgagttg aaggagagc 
      gagacgagc aaaatggac acgatcaag gagttgggc 
      aaaccaaca gggtataag ccgcttctg atcctgact 
      ggacttttc ctgttccag caattttcc gggattttc 
      accttcctg ttttactcg atcacgttt tttcaagag 
      gtgggttca acgatgaat ccctatctc acgtcgatt 
      ttcatcgga atcgtacgc tttgtcatg tgcatggtc 
      aacacctac gtcctgcga acgtacggc aggagaccg 
      ttggtcatc ttgagttgt ttcgggatg tctgtcagc 
      attttcctc tccgggttt ttcacccat tgggtcaaa 
      accggtgtc actacactc acgtggctt cctgtcctc 
      ttcctccta cttttcgtg ttcacttca atgatcggt 
      ttggtccca attccctac acaatgacc gcggaattg 
      ttccccctg gagataaga ggggtagcg cacagtatt 
      tccacgtgt cttgccagt attttcacc tttgcgtct 
      ctacagctg tatccagtg atgtatcaa ggctttggg 
      ggtattcat ggagtccag tatttcttc gcaggggtc 
      actttaata gccgcgttc tacgtctat gtgtttttg 
      cccgaaact caccaaaag aagttgtcc gaaattgag 
      gactatttc aacaaacct ccaaaggct gaaaagcaa 
      caaaaacaa atcgtgcaa gaggtaaat gagacgagc 
      aaaatggac acgatcaag gagttgggc aaaccaaca 
      ggttataag ccgctcttg atcctgact ggacttttc 
      ctgttccag caattttcc gggattttc accttcctg 
      ttttactcg atcacgttc tttcaagag gtgggttca 
      acgatgaat ccctatctc acgtcgatt ttcatcgga 
      atcgtacgc tttgtcatg tgcatggtc aacacctac 
      gtcctgcga acgtacggc aggagaccg ttggtcatt 
      ttgagttgt ttcgggatg tctgtcagc attttcctc 
      tccgggttt ttcacccat tgggtcaaa accggtgtc 
      actacactc acgtggctt cctgtcctc ttcctccta 
      cttttcgtg ttcacttca atgatcggt ttggtccca 
      attccctac acaatgacc gcggaattg ttccccctg 
      gagataaga ggggtagcg cacagtatt tccacgtgt 
      cttgccagt attttcacc tttgcgtct ctacaactg 
      tatccagtg atgtatcaa ggctttggg ggtattcat 
      ggagtccag tatttcttc gcaggggtc actttatta 
      gccgcggtt tacgtctat gtgtttttg cccgaaact 
      caccaaaag aagttgtcc gaaattgag gactatttc 
      aacaaacct acaaagggc attgattca aatcttgct 
      gcggcagtt tatttgacc ggaatcgat gtaattttt 
      tttgcccaa gctttaaca tccctcggg atggtgcta 
      gccttcatc atgggctat ttcctggac tggaaacaa 
      gtcgcctgc ttcacaaac atcttcatc gtaatcccg 
      tgcatcctc gtgttcttc atcccggaa agtcccgcc 
      tggctcgtg tcgaaaaac cggatagaa gaagcgaaa 
      aaatcactg ctttggatc aacaaatac caaacggtg 
      cagttatca ctcgttcag ctctcgctt ctccaaagg 
      gaacacgag ttgaaggag agcgagacg agcaaaatg 
      gacacgatt aaggagttg ggcaaacca acagggtat 
      aagccgctc ttgatcctg actggactt ttcctgttc 
      cagcaattt tccgggatt ttcaccttc ctgttttac 
      tcgatcacg ttctttcaa gaggtgggt tccacgatg 
      aatccctat ctcacgtcg attttcatc ggaatcgta 
      cgctttgtc atgtgcatg gtcaacacc tatgtcctg 
      cgaacgtac ggcaggaga ccgttggtc attttgagt 
      tgtttcggg atgtctgtc agcattttc ctctccggg 
      tttttcacc cattgggtc aaaaccggt gtcactaca 
      ctcacgtgg cttcctgtc cttttcctc ctacttttc 
      gtgttcact tcaatgatc ggtttggtc ccaattccc 
      tacacaatg accgcggaa ttgttcccc ctggagata 
      agaggggta gcgcacagt atttccacg tgtcttgcc 
      agtattttc acctttgcg tctctacaa ctgtatcca 
      gtgatgtat caaggcttt gggggtatt catggagtc 
      cagtatttc ttcgcaggg gtcacttta ttagccgcg 
      gtttacgtc tatgtgttt ttgcccgaa actcaccaa 
      aagaagttg tccgaaatt gaggactat ttcaacaaa 
      cctccaaag gctgaaaag caacagaaa caaatcgtg 
      caagaggtt ttgatttat tttatttta ctattatat 
      ccagtgatc aaacagttg ggcaaacca acagggtat 
      aagccgctc ttgatcctg actggactc ttcctgttc 
      cagcaattt tccgggatt ttcaccttc ctgttttac 
      tcgatcacg ttctttcaa gaggtgggt tcaacgatg 
      aatccctat ctcacgtcg attttcatc ggaatcgta 
      cgctttgtc atgtgcatg gtcaacacc tacgtcctg 
      cgaacgtac ggcaggaga ccgttggtc attttgagt 
      tgtttcggg atgtctgtc agcattttc ctctccggg 
      tttttcacc cattgggtc aaaaccggt gtcactaca 
      ctcacgtgg cttcctgtc ctcttcctc ctacttttc 
      gtgttcact tcaatgatc ggtttggtc ccaattccc 
      tacacaatg accgcggaa ttgttcccc ctggagata 
      agaggggta gcgcacagt atttccacg tgtcttgcc 
      agtattttc acctttgcg tctctacag ctgtatcca 
      gtgatgtat caaggcttt gggggtatt catggagtt 
      cagtatttc ttcgcaggg gtcacttta atagccgcg 
      gtttacgtc tatgtgttt ttgcccgaa acccaccaa 
      aaaaagttg tccgaaatt gaggactat ttcaacaaa 
      cctccaaag ggtgaaaag caacaaaaa caaatcgtg 
      caagaggta taa
    mRNA sequence
    • >TC033468 RA
      taaaatgtc tatggtcga gtgttgttg ttagtttaa 
      gtaagtata agtaactct ccgattttt attgttatt 
      tagtaaaat ggaccctct tagtggaag ttgctacaa 
      tctcaactc acgaagact ctcaagcca gatcctcgc 
      ccaacaaaa aatcgacca aaaatactc acaaatttc 
      aggcagcgt tatcacact cagcaaata tgaacaacg 
      caacttcaa gaatctcac accacaatt actagccgc 
      tgttttagt cacttccta ccatgtctc gattggaat 
      ctcgttggc attctccgc catagtcct cccccagct 
      caaatctca ccccgaatt taaagtcag cgaatgcga 
      ggcgtcatg gattgcaag cattgtcgc cttgagcac 
      ccccctggg aagcctcat cgtgggctt cctcatgga 
      ccaatacgg ccgcctcaa gaccctcgc catggcctc 
      cattcctgc gatcagtgg ctgggtcct gatcgctct 
      ttctgacaa cgtcctttt actgataac aggacgcgc 
      gttggttgg tttcgcctc aagcatcgg ctcgagtcc 
      tgcggtggt ttatttgac cgaaatcgc ccgaaagga 
      catgcgagg atcgttgat ttgtttcgc ccaagcttt 
      aacctccct cgggatggt gctagcctt catcatggg 
      ctatttcct ggactggaa acaagtcgc ctggttcac 
      gaacatctt catcgtaat cccgtgcat cctcgtgtt 
      cttcatccc ggaaagtcc cgcctggct cgtgtcgaa 
      aaaccggat agaagaagc gaaaaaatc actgctttg 
      gatcaacaa ataccaaac ggtgcagtt gtcactcgt 
      tcagctctc gcttctcca aagggaaca cgagttgaa 
      ggagagcga gacgagcaa aatggacac gatcaagga 
      gttgggcaa accaacagg gtataagcc gcttctgat 
      cctgactgg acttttcct gttccagca attttccgg 
      gattttcac cttcctgtt ttactcgat cacgttttt 
      tcaagaggt gggttcaac gatgaatcc ctatctcac 
      gtcgatttt catcggaat cgtacgctt tgtcatgtg 
      catggtcaa cacctacgt cctgcgaac gtacggcag 
      gagaccgtt ggtcatctt gagttgttt cgggatgtc 
      tgtcagcat tttcctctc cgggttttt cacccattg 
      ggtcaaaac cggtgtcac tacactcac gtggcttcc 
      tgtcctctt cctcctact tttcgtgtt cacttcaat 
      gatcggttt ggtcccaat tccctacac aatgaccgc 
      ggaattgtt ccccctgga gataagagg ggtagcgca 
      cagtatttc cacgtgtct tgccagtat tttcacctt 
      tgcgtctct acagctgta tccagtgat gtatcaagg 
      ctttggggg tattcatgg agtccagta tttcttcgc 
      aggggtcac tttaatagc cgcgttcta cgtctatgt 
      gtttttgcc cgaaactca ccaaaagaa gttgtccga 
      aattgagga ctatttcaa caaacctcc aaaggctga 
      aaagcaaca aaaacaaat cgtgcaaga ggtaaatga 
      gacgagcaa aatggacac gatcaagga gttgggcaa 
      accaacagg ttataagcc gctcttgat cctgactgg 
      acttttcct gttccagca attttccgg gattttcac 
      cttcctgtt ttactcgat cacgttctt tcaagaggt 
      gggttcaac gatgaatcc ctatctcac gtcgatttt 
      catcggaat cgtacgctt tgtcatgtg catggtcaa 
      cacctacgt cctgcgaac gtacggcag gagaccgtt 
      ggtcatttt gagttgttt cgggatgtc tgtcagcat 
      tttcctctc cgggttttt cacccattg ggtcaaaac 
      cggtgtcac tacactcac gtggcttcc tgtcctctt 
      cctcctact tttcgtgtt cacttcaat gatcggttt 
      ggtcccaat tccctacac aatgaccgc ggaattgtt 
      ccccctgga gataagagg ggtagcgca cagtatttc 
      cacgtgtct tgccagtat tttcacctt tgcgtctct 
      acaactgta tccagtgat gtatcaagg ctttggggg 
      tattcatgg agtccagta tttcttcgc aggggtcac 
      tttattagc cgcggttta cgtctatgt gtttttgcc 
      cgaaactca ccaaaagaa gttgtccga aattgagga 
      ctatttcaa caaacctac aaagggcat tgattcaaa 
      tcttgctgc ggcagttta tttgaccgg aatcgatgt 
      aattttttt tgcccaagc tttaacatc cctcgggat 
      ggtgctagc cttcatcat gggctattt cctggactg 
      gaaacaagt cgcctgctt cacaaacat cttcatcgt 
      aatcccgtg catcctcgt gttcttcat cccggaaag 
      tcccgcctg gctcgtgtc gaaaaaccg gatagaaga 
      agcgaaaaa atcactgct ttggatcaa caaatacca 
      aacggtgca gttatcact cgttcagct ctcgcttct 
      ccaaaggga acacgagtt gaaggagag cgagacgag 
      caaaatgga cacgattaa ggagttggg caaaccaac 
      agggtataa gccgctctt gatcctgac tggactttt 
      cctgttcca gcaattttc cgggatttt caccttcct 
      gttttactc gatcacgtt ctttcaaga ggtgggttc 
      cacgatgaa tccctatct cacgtcgat tttcatcgg 
      aatcgtacg ctttgtcat gtgcatggt caacaccta 
      tgtcctgcg aacgtacgg caggagacc gttggtcat 
      tttgagttg tttcgggat gtctgtcag cattttcct 
      ctccgggtt tttcaccca ttgggtcaa aaccggtgt 
      cactacact cacgtggct tcctgtcct tttcctcct 
      acttttcgt gttcacttc aatgatcgg tttggtccc 
      aattcccta cacaatgac cgcggaatt gttccccct 
      ggagataag aggggtagc gcacagtat ttccacgtg 
      tcttgccag tattttcac ctttgcgtc tctacaact 
      gtatccagt gatgtatca aggctttgg gggtattca 
      tggagtcca gtatttctt cgcaggggt cactttatt 
      agccgcggt ttacgtcta tgtgttttt gcccgaaac 
      tcaccaaaa gaagttgtc cgaaattga ggactattt 
      caacaaacc tccaaaggc tgaaaagca acagaaaca 
      aatcgtgca agaggtttt gatttattt tattttact 
      attatatcc agtgatcaa acagttggg caaaccaac 
      agggtataa gccgctctt gatcctgac tggactctt 
      cctgttcca gcaattttc cgggatttt caccttcct 
      gttttactc gatcacgtt ctttcaaga ggtgggttc 
      aacgatgaa tccctatct cacgtcgat tttcatcgg 
      aatcgtacg ctttgtcat gtgcatggt caacaccta 
      cgtcctgcg aacgtacgg caggagacc gttggtcat 
      tttgagttg tttcgggat gtctgtcag cattttcct 
      ctccgggtt tttcaccca ttgggtcaa aaccggtgt 
      cactacact cacgtggct tcctgtcct cttcctcct 
      acttttcgt gttcacttc aatgatcgg tttggtccc 
      aattcccta cacaatgac cgcggaatt gttccccct 
      ggagataag aggggtagc gcacagtat ttccacgtg 
      tcttgccag tattttcac ctttgcgtc tctacagct 
      gtatccagt gatgtatca aggctttgg gggtattca 
      tggagttca gtatttctt cgcaggggt cactttaat 
      agccgcggt ttacgtcta tgtgttttt gcccgaaac 
      ccaccaaaa aaagttgtc cgaaattga ggactattt 
      caacaaacc tccaaaggg tgaaaagca acaaaaaca 
      aatcgtgca agaggtata aaatgtaaa gactctatt 
      gtgtgcaat ttttaataa aataaagca ttgtgataa 
      ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY