Status
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Gene information
General information
TC014862 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9596165..9617566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014862
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05709

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014862 RA
      MALFKTDYS RFSWRRKRD KTIQEQHAG LLKGTMQTP 
      GYLMYINKG QDDEMEICC FKTSKIKIV FTYLATVLT 
      GGILRLIFH WIPHWYVFA TCNKCAITQ AEKILIKEI 
      FGGKHKIYH VKNLKVLTP ESVQKLKDD DENLLNSHH 
      VTVKENNPV LSVHFENGE FRDLDKLLM FTCKKVTYI 
      WDSRKFEFV KLRGLDQGV SSDVLHRNK GLTNGEQFT 
      RRLVYGPNK ITVKELSIV TLLFLEVLN PFYIFQIGS 
      FILWFLDDY YYYAAAIIA MSVFGICMT VRQTRKNQR 
      NLKSTVHSS DVCTVLRKI PKNCDGSGD VSYQTESIS 
      TELLVPGDV LVIPSHGCV MHCDAILLT GNCILNESM 
      LTGESVPVT KTALPNLPD LIYDPKEHA RHTLFCGTQ 
      VIQTRYFGN EKVLAVVVR TGFSTAKGS LVRSILYPP 
      PVDFRFEKD SYRFVILLA GVALIGFIY TVVTKVMRG 
      VSSRDLVIE AFDLITIVV PPALPAAMT VGRFYAQIR 
      LKKRKIFCI SPRTINVSG SIDCVCFDK TGTLTEDGL 
      DLLCVVPIK AKNFHMPVK KVSCLPYDT FVCGLVSCH 
      SLTIIDKQI VGDPLDLKM FESTKWVME EHDIADNNK 
      FNMIFPTVL KPPKNRENQ NLDDLQIGI IREFPFSSS 
      SQRMGVIIR KLGAQHFEY YSKGSPEMI LNFVRSDSV 
      PDDFHDVLE SYTQEGYRV IALAHKELK LSYAKVNKV 
      QRDAIEKDM TLLGLIVLE NRLKPETTP CIQALNEAS 
      IRVIMVTGD NILTALSVA KDCDIVTQG QSVITVNSD 
      NSTPPQLYY TLTNTKNKT SNEISVLSN SASVVSLDT 
      IESQIQSIT TNSTVKKME KPHLFNNYR FAMTGKVWG 
      VVKEFYPEL LPRLVTRGT VFARMSPEQ KQQLVQELQ 
      NLGYCVAMC GDGANDCGA LKAAHTGIS LSEAESSVA 
      SPFTSRNPN ISCVLNVIK EGRAALVTS FGIFKYMAA 
      YSLCQFVSV LILYSIDSN LTDLEFLYI DLFIISIFA 
      FFFGKTEAY SGKLVKETP LSSLMSVSP ILSLLLQML 
      LVIGIQVGA FEHLKLQSW YKEFHHNDA HKDEVGCTE 
      NFVIFTVSS FQYIILAVV FSKGKPYRK SIFSNYGFL 
      ITAILMAAF SVYLALWPA QFFIDQFEL VLPEFNFRV 
      YLLGYASVN FILSIFIEQ VLIDYVVFK KLRFKFHNI 
      DKSKRKFLA IERDINLDT KWPVLTSDF KSAASPLNP 
      LPTCTAEIV IEKEKFAKN HVLNKLYDL PNGQLHDDL 
      PFSSLPSES SPSLSDTYK SFPSGNTSY DLANSQWNI 
      AELPYDSAN DSAQNLSSF NSFGTSPKN NKECVKALE 
      MNYLENR
    CDS sequence
    • >TC014862 RA
      atggcgctc ttcaagacg gactattcc cgcttctct 
      tggcgaagg aagcgagac aagaccatt caggagcaa 
      cacgctgga ttgttaaaa ggtaccatg cagacacct 
      ggctacttg atgtacatc aacaaggga caggatgac 
      gaaatggaa atttgctgc ttcaagacg agcaaaatc 
      aaaatcgtt tttacgtac ttagctacg gtacttaca 
      ggtggaatt ttgaggctg atttttcat tggataccg 
      cattggtac gtcttcgcg acgtgcaat aaatgtgca 
      atcactcaa gcggagaaa atcctaatc aaggagata 
      tttggaggt aagcacaag atataccac gtcaaaaac 
      ctgaaagtt ttgacgcct gaaagtgtc caaaaactc 
      aaagacgac gacgagaat ctacttaat tcccatcac 
      gtgaccgtg aaagagaat aaccctgta ttgtctgtg 
      cattttgag aatggggaa tttcgcgat ttggacaag 
      cttctcatg ttcacttgt aaaaaggtg acgtacatc 
      tgggattcc cgcaaattc gagtttgtg aaattgcgc 
      ggtttggat caaggtgtt tcgagtgat gttttgcat 
      agaaacaaa ggattaaca aatggggaa caattcacc 
      cgccgattg gtttacggc ccgaataaa atcacagta 
      aaggagctc tcaattgtg acgcttctt tttttggaa 
      gttcttaac ccgttctat atttttcaa attgggagt 
      tttatttta tggtttttg gatgattat tattactac 
      gccgctgcg attattgca atgtccgtt tttgggatt 
      tgtatgact gtgagacag acgcgaaaa aatcaaagg 
      aatttgaaa tcaacggtt cacagttca gatgtttgt 
      actgttttg aggaaaata ccgaagaat tgtgatggg 
      tctggagat gtgtcgtat cagactgag tccatttcg 
      accgaactt ttagtacca ggggatgtt ctagtgata 
      ccttcacat ggctgtgtt atgcactgt gatgctatt 
      ttactcaca gggaattgt attttgaac gagtccatg 
      ctaacgggt gaatcggtc cccgtgacc aaaaccgcc 
      ctacctaac ctccctgat ctcatctac gaccctaaa 
      gaacacgca cgacatacc ctattttgt ggcactcaa 
      gtcatccaa actcgttac tttggtaat gaaaaagtc 
      cttgcagtg gtggtacgc acgggattt tcgacagcc 
      aaaggaagt ctcgtcagg agtatatta tacccacct 
      ccggtggat ttccgcttc gaaaaggat tcttacaga 
      tttgttatt ttattggcc ggagtggct ttgatcggt 
      tttatatac acagtggtc accaaagtt atgagaggg 
      gtgtcgtca cgtgattta gtaatcgaa gcttttgat 
      ttgataaca attgtggtc ccgcctgca cttcccgcc 
      gccatgaca gttggacgt ttctatgct caaattcgg 
      ttgaaaaag cggaaaata ttttgtatt tccccgagg 
      actatcaac gtttctggc tccatagat tgtgtgtgt 
      ttcgacaag actggtact ttaaccgaa gacggcttg 
      gatctcctc tgtgtggtt ccaatcaaa gcgaagaat 
      tttcacatg cctgtgaag aaagtcagt tgtttgcct 
      tacgacact tttgtttgt ggtcttgtt tcgtgtcac 
      tccttgaca ataattgat aaacaaata gttggtgac 
      ccgctcgat ttgaaaatg tttgaaagt acaaaatgg 
      gtgatggaa gaacacgac attgccgac aacaacaag 
      tttaatatg atttttcca accgtgtta aaaccgcca 
      aaaaatcgt gaaaaccaa aatctggac gaccttcaa 
      atcggaatc attcgagag tttccgttt tcgagcagt 
      tcgcaacga atgggtgta ataatacgg aagttgggg 
      gcccaacat tttgaatac tattcgaaa ggatcgcct 
      gaaatgatc ttgaatttt gtgcgaagt gatagcgtc 
      cctgacgac ttccatgat gtgcttgaa agctacact 
      caggaaggc tatcgggtt atagctctg gcgcacaaa 
      gaattgaaa ctgtcttat gccaaagtg aataaagtc 
      caaagagac gcaatcgag aaagatatg actttattg 
      gggttaatt gtcctcgaa aatcggcta aaacccgaa 
      actacaccc tgtatccag gctttgaat gaggccagt 
      attcgagtt attatggtg acaggtgac aatattctg 
      acagcgttg agcgttgcc aaagattgt gatattgtg 
      acacaaggt cagagtgtc ataactgtg aattctgat 
      aacagtaca ccaccgcaa ctttactac acgttgacc 
      aacacgaaa aataaaaca tcgaatgag attagtgtt 
      ttgtcaaat tctgcgagt gttgtcagt ttggatacg 
      attgagagt caaattcag agcattaca acaaatagt 
      accgtgaaa aaaatggag aaaccgcat ttgtttaac 
      aattatcgg tttgcaatg actgggaaa gtttgggga 
      gtggttaag gagttttat ccggagttg ttgcctcgt 
      ttggtcact agagggaca gtgtttgcg agaatgtcg 
      cccgagcag aagcaacaa ttggtccag gagttgcag 
      aatttggga tattgtgta gcgatgtgt ggcgacggt 
      gcgaacgac tgtggcgcc cttaaagca gcccatacc 
      ggaatttct ctctccgaa gccgaatcg tccgttgca 
      agtccgttt acgagtcga aatccgaac atttcgtgt 
      gtcttgaac gtgatcaaa gaaggccga gcggcactg 
      gtaactagt ttcggtatt tttaaatat atggcagcg 
      tacagtcta tgtcaattc gtctcggtt ttgattttg 
      tacagtatc gattcgaac ctaaccgac ttggagttc 
      ctctacatc gatttgttt atcatttcg atttttgcc 
      tttttcttc ggaaaaacc gaagcgtac agcggaaaa 
      ttggtgaaa gaaacaccc ttaagtagt ctaatgagt 
      gtcagtccg attctttca ctcctgtta caaatgctg 
      ctcgtgatt gggatccaa gttggcgcg tttgaacat 
      ttaaaattg caaagttgg tacaaggag tttcaccat 
      aacgacgcg cataaggac gaagtcggg tgtactgaa 
      aattttgtc atttttaca gtttccagt tttcagtat 
      ataatacta gcggtggtt ttttccaaa ggtaagccg 
      taccgtaag tcgattttt tcgaattat ggttttttg 
      ataactgcg attttaatg gcggcgttt tcggtttat 
      ttggctttg tggcctgcg caatttttc atcgatcag 
      tttgagttg gtactgcct gaatttaac ttccgggtt 
      tatttgctc ggttacgcc tcggttaac tttattctg 
      agcattttt atcgagcaa gtgcttatt gattacgtg 
      gtttttaag aagttgcgc ttcaagttc cacaatatt 
      gataagtcg aagcgaaag tttttagcg attgaacgc 
      gatattaat ttggatacg aagtggcca gttttgacg 
      tccgatttt aaatcggcc gccagtccg ttgaatccg 
      ctgccaact tgcacggct gagattgta attgagaag 
      gaaaagttt gcgaagaat cacgttttg aataagttg 
      tatgattta ccgaatggt caattacat gatgatctt 
      ccgttttcg agtttgcct tccgagtct agtcctagt 
      ttaagcgac acttataag agttttccg agcggaaat 
      actagttac gatttggcc aattcgcag tggaatatt 
      gctgagctt ccgtatgat agtgcgaac gattcggca 
      caaaattta tcgagtttt aactcgttc ggaacaagt 
      ccgaaaaat aataaagag tgtgttaag gcattagaa 
      atgaattat ttagaaaat aggtga
    mRNA sequence
    • >TC014862 RA
      attcaaggc tactggaaa acactgata actgcataa 
      aaagtgttc aacgcactc acttatcaa ttctaagta 
      gaaatttaa tctttagaa taaagataa aaacgataa 
      aagaaaaag caagtgttt gaagtggga ttaacattt 
      tttactaat tgtattaat ttgaggtgc ctgcctaat 
      accaagata acacccatt atcaaaaaa aaatcggat 
      cattgccct aatttgatt attactgaa gataaacgg 
      tgagtgtta atgaattat cttccttat cttttgatt 
      tgaaaataa gcccttcgt gacgttttc tcctaccac 
      acactaatt acgccaatg ttgcctaaa aattttcaa 
      ccgtgatag gacccctcc tcattgaaa acctcacac 
      ttttccgta ttttctcca ggtaatagt gcaacgcca 
      catccattt tgtcacact ttcacgtcc cacattccg 
      cattacatg gacaggtga gataaatcg aattaaacc 
      gcaattcag gctcttaca aattagtca caactgtta 
      gtttttgta caaagtggt aattaagcg tgtaattag 
      ggaaagagg gggcgagat ttgaatttg gcgggcgtt 
      gtggttccg gtcacgtga ctctcccat ctgtcattg 
      gtgtcattt cgtgtggaa tcagaaata tccgtcgtt 
      ttttacggt tgtagtgtt gaaaattaa tccagtgat 
      taaaaatat cgacgtccg taaataatt ccatgtgtc 
      cttcacgca aacgcttaa aatagccag tgcaaagtg 
      cgattatta agtgtgcgg tgtcaagtt gctccaatt 
      ggctttttc ggcgtgttt catgatgaa aatgaagat 
      ttataaggt tttaatatt aattgaagt cattagaag 
      ccccttgac tgtgtttcg tagtgagaa ttcggtcta 
      gataatctc gtgtggaaa agtgaggtt aggtgactc 
      agagaccgt ttttggtgt tttttcgtg gttgatgtt 
      cgataagtt gattattat gtgtaagag tgatctaaa 
      tagatacaa ttatttgag caagtgctt ggataaagt 
      cagcgggat tgttacaaa taagatgca caatgtaga 
      gtcgagctt tgtcacgtg cagcattgg atacagggc 
      gaggaacga tcctgggga ctgacaaaa gttgttcgg 
      aggctgcca ggatggcta tttgacatt gacttgatg 
      ctttccccc tgattgatt cgatgtttg atgtccaga 
      ttttcgata attgtctta accgtgaag attagcttg 
      tggcagttg gcggaagaa tcgaaagag aacgaaacg 
      aacattaaa gtgatggaa tgcgggaaa agttagcag 
      tgttgaaaa agtacttag tttttcgtt gacttgaac 
      tgtacttta ttacttaaa aaaaataca aaaaattaa 
      tcaaataaa atagtgatt taattgtgc aatatggcg 
      ctcttcaag acggactat tcccgcttc tcttggcga 
      aggaagcga gacaagacc attcaggag caacacgct 
      ggattgtta aaaggtacc atgcagaca cctggctac 
      ttgatgtac atcaacaag ggacaggat gacgaaatg 
      gaaatttgc tgcttcaag acgagcaaa atcaaaatc 
      gtttttacg tacttagct acggtactt acaggtgga 
      attttgagg ctgattttt cattggata ccgcattgg 
      tacgtcttc gcgacgtgc aataaatgt gcaatcact 
      caagcggag aaaatccta atcaaggag atatttgga 
      ggtaagcac aagatatac cacgtcaaa aacctgaaa 
      gttttgacg cctgaaagt gtccaaaaa ctcaaagac 
      gacgacgag aatctactt aattcccat cacgtgacc 
      gtgaaagag aataaccct gtattgtct gtgcatttt 
      gagaatggg gaatttcgc gatttggac aagcttctc 
      atgttcact tgtaaaaag gtgacgtac atctgggat 
      tcccgcaaa ttcgagttt gtgaaattg cgcggtttg 
      gatcaaggt gtttcgagt gatgttttg catagaaac 
      aaaggatta acaaatggg gaacaattc acccgccga 
      ttggtttac ggcccgaat aaaatcaca gtaaaggag 
      ctctcaatt gtgacgctt ctttttttg gaagttctt 
      aacccgttc tatattttt caaattggg agttttatt 
      ttatggttt ttggatgat tattattac tacgccgct 
      gcgattatt gcaatgtcc gtttttggg atttgtatg 
      actgtgaga cagacgcga aaaaatcaa aggaatttg 
      aaatcaacg gttcacagt tcagatgtt tgtactgtt 
      ttgaggaaa ataccgaag aattgtgat gggtctgga 
      gatgtgtcg tatcagact gagtccatt tcgaccgaa 
      cttttagta ccaggggat gttctagtg ataccttca 
      catggctgt gttatgcac tgtgatgct attttactc 
      acagggaat tgtattttg aacgagtcc atgctaacg 
      ggtgaatcg gtccccgtg accaaaacc gccctacct 
      aacctccct gatctcatc tacgaccct aaagaacac 
      gcacgacat accctattt tgtggcact caagtcatc 
      caaactcgt tactttggt aatgaaaaa gtccttgca 
      gtggtggta cgcacggga ttttcgaca gccaaagga 
      agtctcgtc aggagtata ttataccca cctccggtg 
      gatttccgc ttcgaaaag gattcttac agatttgtt 
      attttattg gccggagtg gctttgatc ggttttata 
      tacacagtg gtcaccaaa gttatgaga ggggtgtcg 
      tcacgtgat ttagtaatc gaagctttt gatttgata 
      acaattgtg gtcccgcct gcacttccc gccgccatg 
      acagttgga cgtttctat gctcaaatt cggttgaaa 
      aagcggaaa atattttgt atttccccg aggactatc 
      aacgtttct ggctccata gattgtgtg tgtttcgac 
      aagactggt actttaacc gaagacggc ttggatctc 
      ctctgtgtg gttccaatc aaagcgaag aattttcac 
      atgcctgtg aagaaagtc agttgtttg ccttacgac 
      acttttgtt tgtggtctt gtttcgtgt cactccttg 
      acaataatt gataaacaa atagttggt gacccgctc 
      gatttgaaa atgtttgaa agtacaaaa tgggtgatg 
      gaagaacac gacattgcc gacaacaac aagtttaat 
      atgattttt ccaaccgtg ttaaaaccg ccaaaaaat 
      cgtgaaaac caaaatctg gacgacctt caaatcgga 
      atcattcga gagtttccg ttttcgagc agttcgcaa 
      cgaatgggt gtaataata cggaagttg ggggcccaa 
      cattttgaa tactattcg aaaggatcg cctgaaatg 
      atcttgaat tttgtgcga agtgatagc gtccctgac 
      gacttccat gatgtgctt gaaagctac actcaggaa 
      ggctatcgg gttatagct ctggcgcac aaagaattg 
      aaactgtct tatgccaaa gtgaataaa gtccaaaga 
      gacgcaatc gagaaagat atgacttta ttggggtta 
      attgtcctc gaaaatcgg ctaaaaccc gaaactaca 
      ccctgtatc caggctttg aatgaggcc agtattcga 
      gttattatg gtgacaggt gacaatatt ctgacagcg 
      ttgagcgtt gccaaagat tgtgatatt gtgacacaa 
      ggtcagagt gtcataact gtgaattct gataacagt 
      acaccaccg caactttac tacacgttg accaacacg 
      aaaaataaa acatcgaat gagattagt gttttgtca 
      aattctgcg agtgttgtc agtttggat acgattgag 
      agtcaaatt cagagcatt acaacaaat agtaccgtg 
      aaaaaaatg gagaaaccg catttgttt aacaattat 
      cggtttgca atgactggg aaagtttgg ggagtggtt 
      aaggagttt tatccggag ttgttgcct cgtttggtc 
      actagaggg acagtgttt gcgagaatg tcgcccgag 
      cagaagcaa caattggtc caggagttg cagaatttg 
      ggatattgt gtagcgatg tgtggcgac ggtgcgaac 
      gactgtggc gcccttaaa gcagcccat accggaatt 
      tctctctcc gaagccgaa tcgtccgtt gcaagtccg 
      tttacgagt cgaaatccg aacatttcg tgtgtcttg 
      aacgtgatc aaagaaggc cgagcggca ctggtaact 
      agtttcggt atttttaaa tatatggca gcgtacagt 
      ctatgtcaa ttcgtctcg gttttgatt ttgtacagt 
      atcgattcg aacctaacc gacttggag ttcctctac 
      atcgatttg tttatcatt tcgattttt gcctttttc 
      ttcggaaaa accgaagcg tacagcgga aaattggtg 
      aaagaaaca cccttaagt agtctaatg agtgtcagt 
      ccgattctt tcactcctg ttacaaatg ctgctcgtg 
      attgggatc caagttggc gcgtttgaa catttaaaa 
      ttgcaaagt tggtacaag gagtttcac cataacgac 
      gcgcataag gacgaagtc gggtgtact gaaaatttt 
      gtcattttt acagtttcc agttttcag tatataata 
      ctagcggtg gttttttcc aaaggtaag ccgtaccgt 
      aagtcgatt ttttcgaat tatggtttt ttgataact 
      gcgatttta atggcggcg ttttcggtt tatttggct 
      ttgtggcct gcgcaattt ttcatcgat cagtttgag 
      ttggtactg cctgaattt aacttccgg gtttatttg 
      ctcggttac gcctcggtt aactttatt ctgagcatt 
      tttatcgag caagtgctt attgattac gtggttttt 
      aagaagttg cgcttcaag ttccacaat attgataag 
      tcgaagcga aagttttta gcgattgaa cgcgatatt 
      aatttggat acgaagtgg ccagttttg acgtccgat 
      tttaaatcg gccgccagt ccgttgaat ccgctgcca 
      acttgcacg gctgagatt gtaattgag aaggaaaag 
      tttgcgaag aatcacgtt ttgaataag ttgtatgat 
      ttaccgaat ggtcaatta catgatgat cttccgttt 
      tcgagtttg ccttccgag tctagtcct agtttaagc 
      gacacttat aagagtttt ccgagcgga aatactagt 
      tacgatttg gccaattcg cagtggaat attgctgag 
      cttccgtat gatagtgcg aacgattcg gcacaaaat 
      ttatcgagt tttaactcg ttcggaaca agtccgaaa 
      aataataaa gagtgtgtt aaggcatta gaaatgaat 
      tatttagaa aataggtga ggtatagtt ttaggttta 
      gcgaacggg cattttgac ccacaagta ttaaatgta 
      acagtgtat ctcttagtg ttctttata gaagaattt 
      atttatcta cacttaatt catatcatt gagggtttt 
      acggcttta ctaaaccta agttccttt atttttaca 
      gttttataa attgtaatt tttagactt aattgatgt 
      gtacttagc ggagaataa atagttatt tgtttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY