| General information | |||
| TC014862 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 9596165..9617566 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014862 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05709 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC014862 RA
MALFKTDYS RFSWRRKRD KTIQEQHAG LLKGTMQTP GYLMYINKG QDDEMEICC FKTSKIKIV FTYLATVLT GGILRLIFH WIPHWYVFA TCNKCAITQ AEKILIKEI FGGKHKIYH VKNLKVLTP ESVQKLKDD DENLLNSHH VTVKENNPV LSVHFENGE FRDLDKLLM FTCKKVTYI WDSRKFEFV KLRGLDQGV SSDVLHRNK GLTNGEQFT RRLVYGPNK ITVKELSIV TLLFLEVLN PFYIFQIGS FILWFLDDY YYYAAAIIA MSVFGICMT VRQTRKNQR NLKSTVHSS DVCTVLRKI PKNCDGSGD VSYQTESIS TELLVPGDV LVIPSHGCV MHCDAILLT GNCILNESM LTGESVPVT KTALPNLPD LIYDPKEHA RHTLFCGTQ VIQTRYFGN EKVLAVVVR TGFSTAKGS LVRSILYPP PVDFRFEKD SYRFVILLA GVALIGFIY TVVTKVMRG VSSRDLVIE AFDLITIVV PPALPAAMT VGRFYAQIR LKKRKIFCI SPRTINVSG SIDCVCFDK TGTLTEDGL DLLCVVPIK AKNFHMPVK KVSCLPYDT FVCGLVSCH SLTIIDKQI VGDPLDLKM FESTKWVME EHDIADNNK FNMIFPTVL KPPKNRENQ NLDDLQIGI IREFPFSSS SQRMGVIIR KLGAQHFEY YSKGSPEMI LNFVRSDSV PDDFHDVLE SYTQEGYRV IALAHKELK LSYAKVNKV QRDAIEKDM TLLGLIVLE NRLKPETTP CIQALNEAS IRVIMVTGD NILTALSVA KDCDIVTQG QSVITVNSD NSTPPQLYY TLTNTKNKT SNEISVLSN SASVVSLDT IESQIQSIT TNSTVKKME KPHLFNNYR FAMTGKVWG VVKEFYPEL LPRLVTRGT VFARMSPEQ KQQLVQELQ NLGYCVAMC GDGANDCGA LKAAHTGIS LSEAESSVA SPFTSRNPN ISCVLNVIK EGRAALVTS FGIFKYMAA YSLCQFVSV LILYSIDSN LTDLEFLYI DLFIISIFA FFFGKTEAY SGKLVKETP LSSLMSVSP ILSLLLQML LVIGIQVGA FEHLKLQSW YKEFHHNDA HKDEVGCTE NFVIFTVSS FQYIILAVV FSKGKPYRK SIFSNYGFL ITAILMAAF SVYLALWPA QFFIDQFEL VLPEFNFRV YLLGYASVN FILSIFIEQ VLIDYVVFK KLRFKFHNI DKSKRKFLA IERDINLDT KWPVLTSDF KSAASPLNP LPTCTAEIV IEKEKFAKN HVLNKLYDL PNGQLHDDL PFSSLPSES SPSLSDTYK SFPSGNTSY DLANSQWNI AELPYDSAN DSAQNLSSF NSFGTSPKN NKECVKALE MNYLENR
- >TC014862 RA
atggcgctc ttcaagacg gactattcc cgcttctct tggcgaagg aagcgagac aagaccatt caggagcaa cacgctgga ttgttaaaa ggtaccatg cagacacct ggctacttg atgtacatc aacaaggga caggatgac gaaatggaa atttgctgc ttcaagacg agcaaaatc aaaatcgtt tttacgtac ttagctacg gtacttaca ggtggaatt ttgaggctg atttttcat tggataccg cattggtac gtcttcgcg acgtgcaat aaatgtgca atcactcaa gcggagaaa atcctaatc aaggagata tttggaggt aagcacaag atataccac gtcaaaaac ctgaaagtt ttgacgcct gaaagtgtc caaaaactc aaagacgac gacgagaat ctacttaat tcccatcac gtgaccgtg aaagagaat aaccctgta ttgtctgtg cattttgag aatggggaa tttcgcgat ttggacaag cttctcatg ttcacttgt aaaaaggtg acgtacatc tgggattcc cgcaaattc gagtttgtg aaattgcgc ggtttggat caaggtgtt tcgagtgat gttttgcat agaaacaaa ggattaaca aatggggaa caattcacc cgccgattg gtttacggc ccgaataaa atcacagta aaggagctc tcaattgtg acgcttctt tttttggaa gttcttaac ccgttctat atttttcaa attgggagt tttatttta tggtttttg gatgattat tattactac gccgctgcg attattgca atgtccgtt tttgggatt tgtatgact gtgagacag acgcgaaaa aatcaaagg aatttgaaa tcaacggtt cacagttca gatgtttgt actgttttg aggaaaata ccgaagaat tgtgatggg tctggagat gtgtcgtat cagactgag tccatttcg accgaactt ttagtacca ggggatgtt ctagtgata ccttcacat ggctgtgtt atgcactgt gatgctatt ttactcaca gggaattgt attttgaac gagtccatg ctaacgggt gaatcggtc cccgtgacc aaaaccgcc ctacctaac ctccctgat ctcatctac gaccctaaa gaacacgca cgacatacc ctattttgt ggcactcaa gtcatccaa actcgttac tttggtaat gaaaaagtc cttgcagtg gtggtacgc acgggattt tcgacagcc aaaggaagt ctcgtcagg agtatatta tacccacct ccggtggat ttccgcttc gaaaaggat tcttacaga tttgttatt ttattggcc ggagtggct ttgatcggt tttatatac acagtggtc accaaagtt atgagaggg gtgtcgtca cgtgattta gtaatcgaa gcttttgat ttgataaca attgtggtc ccgcctgca cttcccgcc gccatgaca gttggacgt ttctatgct caaattcgg ttgaaaaag cggaaaata ttttgtatt tccccgagg actatcaac gtttctggc tccatagat tgtgtgtgt ttcgacaag actggtact ttaaccgaa gacggcttg gatctcctc tgtgtggtt ccaatcaaa gcgaagaat tttcacatg cctgtgaag aaagtcagt tgtttgcct tacgacact tttgtttgt ggtcttgtt tcgtgtcac tccttgaca ataattgat aaacaaata gttggtgac ccgctcgat ttgaaaatg tttgaaagt acaaaatgg gtgatggaa gaacacgac attgccgac aacaacaag tttaatatg atttttcca accgtgtta aaaccgcca aaaaatcgt gaaaaccaa aatctggac gaccttcaa atcggaatc attcgagag tttccgttt tcgagcagt tcgcaacga atgggtgta ataatacgg aagttgggg gcccaacat tttgaatac tattcgaaa ggatcgcct gaaatgatc ttgaatttt gtgcgaagt gatagcgtc cctgacgac ttccatgat gtgcttgaa agctacact caggaaggc tatcgggtt atagctctg gcgcacaaa gaattgaaa ctgtcttat gccaaagtg aataaagtc caaagagac gcaatcgag aaagatatg actttattg gggttaatt gtcctcgaa aatcggcta aaacccgaa actacaccc tgtatccag gctttgaat gaggccagt attcgagtt attatggtg acaggtgac aatattctg acagcgttg agcgttgcc aaagattgt gatattgtg acacaaggt cagagtgtc ataactgtg aattctgat aacagtaca ccaccgcaa ctttactac acgttgacc aacacgaaa aataaaaca tcgaatgag attagtgtt ttgtcaaat tctgcgagt gttgtcagt ttggatacg attgagagt caaattcag agcattaca acaaatagt accgtgaaa aaaatggag aaaccgcat ttgtttaac aattatcgg tttgcaatg actgggaaa gtttgggga gtggttaag gagttttat ccggagttg ttgcctcgt ttggtcact agagggaca gtgtttgcg agaatgtcg cccgagcag aagcaacaa ttggtccag gagttgcag aatttggga tattgtgta gcgatgtgt ggcgacggt gcgaacgac tgtggcgcc cttaaagca gcccatacc ggaatttct ctctccgaa gccgaatcg tccgttgca agtccgttt acgagtcga aatccgaac atttcgtgt gtcttgaac gtgatcaaa gaaggccga gcggcactg gtaactagt ttcggtatt tttaaatat atggcagcg tacagtcta tgtcaattc gtctcggtt ttgattttg tacagtatc gattcgaac ctaaccgac ttggagttc ctctacatc gatttgttt atcatttcg atttttgcc tttttcttc ggaaaaacc gaagcgtac agcggaaaa ttggtgaaa gaaacaccc ttaagtagt ctaatgagt gtcagtccg attctttca ctcctgtta caaatgctg ctcgtgatt gggatccaa gttggcgcg tttgaacat ttaaaattg caaagttgg tacaaggag tttcaccat aacgacgcg cataaggac gaagtcggg tgtactgaa aattttgtc atttttaca gtttccagt tttcagtat ataatacta gcggtggtt ttttccaaa ggtaagccg taccgtaag tcgattttt tcgaattat ggttttttg ataactgcg attttaatg gcggcgttt tcggtttat ttggctttg tggcctgcg caatttttc atcgatcag tttgagttg gtactgcct gaatttaac ttccgggtt tatttgctc ggttacgcc tcggttaac tttattctg agcattttt atcgagcaa gtgcttatt gattacgtg gtttttaag aagttgcgc ttcaagttc cacaatatt gataagtcg aagcgaaag tttttagcg attgaacgc gatattaat ttggatacg aagtggcca gttttgacg tccgatttt aaatcggcc gccagtccg ttgaatccg ctgccaact tgcacggct gagattgta attgagaag gaaaagttt gcgaagaat cacgttttg aataagttg tatgattta ccgaatggt caattacat gatgatctt ccgttttcg agtttgcct tccgagtct agtcctagt ttaagcgac acttataag agttttccg agcggaaat actagttac gatttggcc aattcgcag tggaatatt gctgagctt ccgtatgat agtgcgaac gattcggca caaaattta tcgagtttt aactcgttc ggaacaagt ccgaaaaat aataaagag tgtgttaag gcattagaa atgaattat ttagaaaat aggtga
- >TC014862 RA
attcaaggc tactggaaa acactgata actgcataa aaagtgttc aacgcactc acttatcaa ttctaagta gaaatttaa tctttagaa taaagataa aaacgataa aagaaaaag caagtgttt gaagtggga ttaacattt tttactaat tgtattaat ttgaggtgc ctgcctaat accaagata acacccatt atcaaaaaa aaatcggat cattgccct aatttgatt attactgaa gataaacgg tgagtgtta atgaattat cttccttat cttttgatt tgaaaataa gcccttcgt gacgttttc tcctaccac acactaatt acgccaatg ttgcctaaa aattttcaa ccgtgatag gacccctcc tcattgaaa acctcacac ttttccgta ttttctcca ggtaatagt gcaacgcca catccattt tgtcacact ttcacgtcc cacattccg cattacatg gacaggtga gataaatcg aattaaacc gcaattcag gctcttaca aattagtca caactgtta gtttttgta caaagtggt aattaagcg tgtaattag ggaaagagg gggcgagat ttgaatttg gcgggcgtt gtggttccg gtcacgtga ctctcccat ctgtcattg gtgtcattt cgtgtggaa tcagaaata tccgtcgtt ttttacggt tgtagtgtt gaaaattaa tccagtgat taaaaatat cgacgtccg taaataatt ccatgtgtc cttcacgca aacgcttaa aatagccag tgcaaagtg cgattatta agtgtgcgg tgtcaagtt gctccaatt ggctttttc ggcgtgttt catgatgaa aatgaagat ttataaggt tttaatatt aattgaagt cattagaag ccccttgac tgtgtttcg tagtgagaa ttcggtcta gataatctc gtgtggaaa agtgaggtt aggtgactc agagaccgt ttttggtgt tttttcgtg gttgatgtt cgataagtt gattattat gtgtaagag tgatctaaa tagatacaa ttatttgag caagtgctt ggataaagt cagcgggat tgttacaaa taagatgca caatgtaga gtcgagctt tgtcacgtg cagcattgg atacagggc gaggaacga tcctgggga ctgacaaaa gttgttcgg aggctgcca ggatggcta tttgacatt gacttgatg ctttccccc tgattgatt cgatgtttg atgtccaga ttttcgata attgtctta accgtgaag attagcttg tggcagttg gcggaagaa tcgaaagag aacgaaacg aacattaaa gtgatggaa tgcgggaaa agttagcag tgttgaaaa agtacttag tttttcgtt gacttgaac tgtacttta ttacttaaa aaaaataca aaaaattaa tcaaataaa atagtgatt taattgtgc aatatggcg ctcttcaag acggactat tcccgcttc tcttggcga aggaagcga gacaagacc attcaggag caacacgct ggattgtta aaaggtacc atgcagaca cctggctac ttgatgtac atcaacaag ggacaggat gacgaaatg gaaatttgc tgcttcaag acgagcaaa atcaaaatc gtttttacg tacttagct acggtactt acaggtgga attttgagg ctgattttt cattggata ccgcattgg tacgtcttc gcgacgtgc aataaatgt gcaatcact caagcggag aaaatccta atcaaggag atatttgga ggtaagcac aagatatac cacgtcaaa aacctgaaa gttttgacg cctgaaagt gtccaaaaa ctcaaagac gacgacgag aatctactt aattcccat cacgtgacc gtgaaagag aataaccct gtattgtct gtgcatttt gagaatggg gaatttcgc gatttggac aagcttctc atgttcact tgtaaaaag gtgacgtac atctgggat tcccgcaaa ttcgagttt gtgaaattg cgcggtttg gatcaaggt gtttcgagt gatgttttg catagaaac aaaggatta acaaatggg gaacaattc acccgccga ttggtttac ggcccgaat aaaatcaca gtaaaggag ctctcaatt gtgacgctt ctttttttg gaagttctt aacccgttc tatattttt caaattggg agttttatt ttatggttt ttggatgat tattattac tacgccgct gcgattatt gcaatgtcc gtttttggg atttgtatg actgtgaga cagacgcga aaaaatcaa aggaatttg aaatcaacg gttcacagt tcagatgtt tgtactgtt ttgaggaaa ataccgaag aattgtgat gggtctgga gatgtgtcg tatcagact gagtccatt tcgaccgaa cttttagta ccaggggat gttctagtg ataccttca catggctgt gttatgcac tgtgatgct attttactc acagggaat tgtattttg aacgagtcc atgctaacg ggtgaatcg gtccccgtg accaaaacc gccctacct aacctccct gatctcatc tacgaccct aaagaacac gcacgacat accctattt tgtggcact caagtcatc caaactcgt tactttggt aatgaaaaa gtccttgca gtggtggta cgcacggga ttttcgaca gccaaagga agtctcgtc aggagtata ttataccca cctccggtg gatttccgc ttcgaaaag gattcttac agatttgtt attttattg gccggagtg gctttgatc ggttttata tacacagtg gtcaccaaa gttatgaga ggggtgtcg tcacgtgat ttagtaatc gaagctttt gatttgata acaattgtg gtcccgcct gcacttccc gccgccatg acagttgga cgtttctat gctcaaatt cggttgaaa aagcggaaa atattttgt atttccccg aggactatc aacgtttct ggctccata gattgtgtg tgtttcgac aagactggt actttaacc gaagacggc ttggatctc ctctgtgtg gttccaatc aaagcgaag aattttcac atgcctgtg aagaaagtc agttgtttg ccttacgac acttttgtt tgtggtctt gtttcgtgt cactccttg acaataatt gataaacaa atagttggt gacccgctc gatttgaaa atgtttgaa agtacaaaa tgggtgatg gaagaacac gacattgcc gacaacaac aagtttaat atgattttt ccaaccgtg ttaaaaccg ccaaaaaat cgtgaaaac caaaatctg gacgacctt caaatcgga atcattcga gagtttccg ttttcgagc agttcgcaa cgaatgggt gtaataata cggaagttg ggggcccaa cattttgaa tactattcg aaaggatcg cctgaaatg atcttgaat tttgtgcga agtgatagc gtccctgac gacttccat gatgtgctt gaaagctac actcaggaa ggctatcgg gttatagct ctggcgcac aaagaattg aaactgtct tatgccaaa gtgaataaa gtccaaaga gacgcaatc gagaaagat atgacttta ttggggtta attgtcctc gaaaatcgg ctaaaaccc gaaactaca ccctgtatc caggctttg aatgaggcc agtattcga gttattatg gtgacaggt gacaatatt ctgacagcg ttgagcgtt gccaaagat tgtgatatt gtgacacaa ggtcagagt gtcataact gtgaattct gataacagt acaccaccg caactttac tacacgttg accaacacg aaaaataaa acatcgaat gagattagt gttttgtca aattctgcg agtgttgtc agtttggat acgattgag agtcaaatt cagagcatt acaacaaat agtaccgtg aaaaaaatg gagaaaccg catttgttt aacaattat cggtttgca atgactggg aaagtttgg ggagtggtt aaggagttt tatccggag ttgttgcct cgtttggtc actagaggg acagtgttt gcgagaatg tcgcccgag cagaagcaa caattggtc caggagttg cagaatttg ggatattgt gtagcgatg tgtggcgac ggtgcgaac gactgtggc gcccttaaa gcagcccat accggaatt tctctctcc gaagccgaa tcgtccgtt gcaagtccg tttacgagt cgaaatccg aacatttcg tgtgtcttg aacgtgatc aaagaaggc cgagcggca ctggtaact agtttcggt atttttaaa tatatggca gcgtacagt ctatgtcaa ttcgtctcg gttttgatt ttgtacagt atcgattcg aacctaacc gacttggag ttcctctac atcgatttg tttatcatt tcgattttt gcctttttc ttcggaaaa accgaagcg tacagcgga aaattggtg aaagaaaca cccttaagt agtctaatg agtgtcagt ccgattctt tcactcctg ttacaaatg ctgctcgtg attgggatc caagttggc gcgtttgaa catttaaaa ttgcaaagt tggtacaag gagtttcac cataacgac gcgcataag gacgaagtc gggtgtact gaaaatttt gtcattttt acagtttcc agttttcag tatataata ctagcggtg gttttttcc aaaggtaag ccgtaccgt aagtcgatt ttttcgaat tatggtttt ttgataact gcgatttta atggcggcg ttttcggtt tatttggct ttgtggcct gcgcaattt ttcatcgat cagtttgag ttggtactg cctgaattt aacttccgg gtttatttg ctcggttac gcctcggtt aactttatt ctgagcatt tttatcgag caagtgctt attgattac gtggttttt aagaagttg cgcttcaag ttccacaat attgataag tcgaagcga aagttttta gcgattgaa cgcgatatt aatttggat acgaagtgg ccagttttg acgtccgat tttaaatcg gccgccagt ccgttgaat ccgctgcca acttgcacg gctgagatt gtaattgag aaggaaaag tttgcgaag aatcacgtt ttgaataag ttgtatgat ttaccgaat ggtcaatta catgatgat cttccgttt tcgagtttg ccttccgag tctagtcct agtttaagc gacacttat aagagtttt ccgagcgga aatactagt tacgatttg gccaattcg cagtggaat attgctgag cttccgtat gatagtgcg aacgattcg gcacaaaat ttatcgagt tttaactcg ttcggaaca agtccgaaa aataataaa gagtgtgtt aaggcatta gaaatgaat tatttagaa aataggtga ggtatagtt ttaggttta gcgaacggg cattttgac ccacaagta ttaaatgta acagtgtat ctcttagtg ttctttata gaagaattt atttatcta cacttaatt catatcatt gagggtttt acggcttta ctaaaccta agttccttt atttttaca gttttataa attgtaatt tttagactt aattgatgt gtacttagc ggagaataa atagttatt tgtttg
