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Gene information
General information
TC014860 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9628144..9635252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014860
    iBeetle screen iB_05708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Comment: all partially cannibalized, no exact examination possible

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014860 RA
      MSPFRKKKS EKSFPVKVC TLDAELEFN LQWRATGRD 
      LFELVCRTI GLRETWYFG LQYEDSKGF INWLKLDKK 
      VQDQSIQKD PHSTVSFMF FAKFYPEEV AEELVQEVT 
      KHYFFLQVK QAILSMDVY CPPEASVLL ASYAVQAKF 
      GDFDLDTYK PGMLANEDL LPQRVIDQY QMTLDMWEE 
      RIRVWYSDH RGMSRDEAE MEYLKIAQD LDMYGVNYF 
      PIMNKKETE LWLGVTPLG LNIYEKENK LQPKTTFTW 
      AEIRHISFD DKKFIIKPV DKNSPNFVF FSQKVRMNK 
      LILDLCMGN HDLFMRRRK PDSMELQQM KAAAKEEKQ 
      RRQIERNRL AREKQLREE AERERANME QRLLQYQEE 
      IRLANEALK RSEESADLL AEKSRVAEE EAILLSQKA 
      AEAEQEITR LRLSAMRKE EEKVTLERK TREAELLTA 
      RLVEDSERR AAEANRLKE ELLRARAAE KQAKEKLLD 
      FLSRGSYSS SATSVSTLY SASLSPLGP DLSLLDGDV 
      GTSPELNLT LNGYDLTNA DTDQLSLEI EKERGLCLE 
      KQKHLQHQL RELRTEIAV LKVAEKQTE FDQLHSEQV 
      KLGENKYST LKKSKSGST KSRVAFFEE L
    CDS sequence
    • >TC014860 RA
      atgtctcca tttcgcaaa aagaaatcc gagaagagt 
      ttccccgtg aaagtgtgc acattggac gccgaattg 
      gagttcaac ttacaatgg cgagccacc ggcagggat 
      ttattcgag ctagtgtgc cgcacaatc gggttacgc 
      gagacttgg tatttcggt ttgcaatac gaagattca 
      aaagggttt atcaattgg ttaaaattg gacaagaaa 
      gtccaagac cagagcatc cagaaggac ccccacagt 
      acggtgtcg ttcatgttt ttcgcgaaa ttctacccc 
      gaagaagtg gccgaagag ctggttcaa gaggtaaca 
      aaacactat ttttttctc caagttaag caagctatt 
      ctctctatg gatgtgtac tgcccccct gaggcgtcc 
      gtcctgcta gccagttac gcggtccaa gccaagttt 
      ggagacttt gacctagat acgtacaaa ccgggaatg 
      ttagcgaac gaggatttg ttgccccaa agagtcatc 
      gaccagtat cagatgact ctagacatg tgggaggag 
      cggataaga gtgtggtac tcagatcac aggggtatg 
      tcgcgagac gaagccgag atggagtat ttaaaaatc 
      gcacaagac ttggacatg tacggtgtt aattatttt 
      ccgataatg aataaaaaa gagactgag ttgtggtta 
      ggtgtaaca cctctagga ttaaacatc tacgaaaag 
      gaaaataaa ctacaacca aaaacgacg ttcacatgg 
      gccgaaata cgacacatt agttttgac gataaaaaa 
      tttattatc aaaccggtc gataagaat tccccaaat 
      tttgtcttc tttagccaa aaagttaga atgaataaa 
      ttgattctt gatttgtgc atgggtaac catgattta 
      ttcatgcgg aggcgtaaa cccgattct atggaactc 
      caacaaatg aaagccgcc gccaaagaa gagaaacaa 
      cgtcggcag attgaacga aatcgtctg gcacgtgaa 
      aaacaattg agagaggag gcggagcgt gaaagggcc 
      aatatggaa cagcgcctg ctccagtac caggaggaa 
      atccgattg gctaatgaa gctctgaaa cgttccgaa 
      gaaagcgcc gatttgctg gccgaaaaa agccgagtt 
      gctgaagaa gaagcaatt cttctgagc cagaaagcg 
      gccgaagcg gaacaagaa atcacccgt ttgcgcctc 
      agcgcgatg cgaaaggaa gaagaaaaa gtcacattg 
      gagcgaaaa actcgtgaa gcagaatta ttaacagct 
      cgattagtt gaagattcg gaacgaaga gcggctgaa 
      gcgaaccga ctgaaagaa gaattgttg cgggcgcga 
      gctgctgaa aaacaagcc aaagaaaaa ttgttggat 
      tttttgagt cgcggctct tattcgtcg tctgcaacg 
      tcagtttcg accctatat tcggcctcg ttgagtcct 
      ctggggccg gatttgtcg cttttggat ggcgatgtc 
      gggacttcc ccggaactt aacctcacg ctgaatggt 
      tacgatttg acgaacgcg gacaccgat caattgtcg 
      ctcgaaatt gagaaggag agaggcttg tgtttggaa 
      aaacagaag catttgcaa caccagcta cgcgagctt 
      cgcaccgaa atcgctgtt ttgaaggtg gcggaaaaa 
      cagaccgag tttgaccaa ctgcacagc gagcaagtg 
      aaattaggc gaaaataaa tactcgacg ttgaaaaag 
      agcaaatct gggtcgacg aagtcgcgg gtggccttc 
      ttcgaagaa ctgtaa
    mRNA sequence
    • >TC014860 RA
      ctggggccc catcgcccg aaagtccta gtaatttcc 
      cgccattgt gttggtatt ccgaattga catctgtca 
      atgccataa ccgagatgt ttgtgcctt atttgttgt 
      tgttatttt acggttgaa taatgcgat aggttttac 
      aaaagcgag gattatgtt agaaaaaat gtctccatt 
      tcgcaaaaa gaaatccga gaagagttt ccccgtgaa 
      agtgtgcac attggacgc cgaattgga gttcaactt 
      acaatggcg agccaccgg cagggattt attcgagct 
      agtgtgccg cacaatcgg gttacgcga gacttggta 
      tttcggttt gcaatacga agattcaaa agggtttat 
      caattggtt aaaattgga caagaaagt ccaagacca 
      gagcatcca gaaggaccc ccacagtac ggtgtcgtt 
      catgttttt cgcgaaatt ctaccccga agaagtggc 
      cgaagagct ggttcaaga ggtaacaaa acactattt 
      ttttctcca agttaagca agctattct ctctatgga 
      tgtgtactg cccccctga ggcgtccgt cctgctagc 
      cagttacgc ggtccaagc caagtttgg agactttga 
      cctagatac gtacaaacc gggaatgtt agcgaacga 
      ggatttgtt gccccaaag agtcatcga ccagtatca 
      gatgactct agacatgtg ggaggagcg gataagagt 
      gtggtactc agatcacag gggtatgtc gcgagacga 
      agccgagat ggagtattt aaaaatcgc acaagactt 
      ggacatgta cggtgttaa ttattttcc gataatgaa 
      taaaaaaga gactgagtt gtggttagg tgtaacacc 
      tctaggatt aaacatcta cgaaaagga aaataaact 
      acaaccaaa aacgacgtt cacatgggc cgaaatacg 
      acacattag ttttgacga taaaaaatt tattatcaa 
      accggtcga taagaattc cccaaattt tgtcttctt 
      tagccaaaa agttagaat gaataaatt gattcttga 
      tttgtgcat gggtaacca tgatttatt catgcggag 
      gcgtaaacc cgattctat ggaactcca acaaatgaa 
      agccgccgc caaagaaga gaaacaacg tcggcagat 
      tgaacgaaa tcgtctggc acgtgaaaa acaattgag 
      agaggaggc ggagcgtga aagggccaa tatggaaca 
      gcgcctgct ccagtacca ggaggaaat ccgattggc 
      taatgaagc tctgaaacg ttccgaaga aagcgccga 
      tttgctggc cgaaaaaag ccgagttgc tgaagaaga 
      agcaattct tctgagcca gaaagcggc cgaagcgga 
      acaagaaat cacccgttt gcgcctcag cgcgatgcg 
      aaaggaaga agaaaaagt cacattgga gcgaaaaac 
      tcgtgaagc agaattatt aacagctcg attagttga 
      agattcgga acgaagagc ggctgaagc gaaccgact 
      gaaagaaga attgttgcg ggcgcgagc tgctgaaaa 
      acaagccaa agaaaaatt gttggattt tttgagtcg 
      cggctctta ttcgtcgtc tgcaacgtc agtttcgac 
      cctatattc ggcctcgtt gagtcctct ggggccgga 
      tttgtcgct tttggatgg cgatgtcgg gacttcccc 
      ggaacttaa cctcacgct gaatggtta cgatttgac 
      gaacgcgga caccgatca attgtcgct cgaaattga 
      gaaggagag aggcttgtg tttggaaaa acagaagca 
      tttgcaaca ccagctacg cgagcttcg caccgaaat 
      cgctgtttt gaaggtggc ggaaaaaca gaccgagtt 
      tgaccaact gcacagcga gcaagtgaa attaggcga 
      aaataaata ctcgacgtt gaaaaagag caaatctgg 
      gtcgacgaa gtcgcgggt ggccttctt cgaagaact 
      gtaatttta ttattttta ataaaaatc tattatttt 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY