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Gene information
General information
TC014860 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9628144..9635252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014860
    iBeetle screen iB_05708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection
    Comment: all partially cannibalized, no exact examination possible

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014860 RA
      MSPFRKKKS EKSFPVKVC TLDAELEFN LQWRATGRD 
LFELVCRTI GLRETWYFG LQYEDSKGF INWLKLDKK 
VQDQSIQKD PHSTVSFMF FAKFYPEEV AEELVQEVT 
KHYFFLQVK QAILSMDVY CPPEASVLL ASYAVQAKF 
GDFDLDTYK PGMLANEDL LPQRVIDQY QMTLDMWEE 
RIRVWYSDH RGMSRDEAE MEYLKIAQD LDMYGVNYF 
PIMNKKETE LWLGVTPLG LNIYEKENK LQPKTTFTW 
AEIRHISFD DKKFIIKPV DKNSPNFVF FSQKVRMNK 
LILDLCMGN HDLFMRRRK PDSMELQQM KAAAKEEKQ 
RRQIERNRL AREKQLREE AERERANME QRLLQYQEE 
IRLANEALK RSEESADLL AEKSRVAEE EAILLSQKA 
AEAEQEITR LRLSAMRKE EEKVTLERK TREAELLTA 
RLVEDSERR AAEANRLKE ELLRARAAE KQAKEKLLD 
FLSRGSYSS SATSVSTLY SASLSPLGP DLSLLDGDV 
GTSPELNLT LNGYDLTNA DTDQLSLEI EKERGLCLE 
KQKHLQHQL RELRTEIAV LKVAEKQTE FDQLHSEQV 
KLGENKYST LKKSKSGST KSRVAFFEE L
    CDS sequence
    • >TC014860 RA
      atgtctcca tttcgcaaa aagaaatcc gagaagagt 
ttccccgtg aaagtgtgc acattggac gccgaattg 
gagttcaac ttacaatgg cgagccacc ggcagggat 
ttattcgag ctagtgtgc cgcacaatc gggttacgc 
gagacttgg tatttcggt ttgcaatac gaagattca 
aaagggttt atcaattgg ttaaaattg gacaagaaa 
gtccaagac cagagcatc cagaaggac ccccacagt 
acggtgtcg ttcatgttt ttcgcgaaa ttctacccc 
gaagaagtg gccgaagag ctggttcaa gaggtaaca 
aaacactat ttttttctc caagttaag caagctatt 
ctctctatg gatgtgtac tgcccccct gaggcgtcc 
gtcctgcta gccagttac gcggtccaa gccaagttt 
ggagacttt gacctagat acgtacaaa ccgggaatg 
ttagcgaac gaggatttg ttgccccaa agagtcatc 
gaccagtat cagatgact ctagacatg tgggaggag 
cggataaga gtgtggtac tcagatcac aggggtatg 
tcgcgagac gaagccgag atggagtat ttaaaaatc 
gcacaagac ttggacatg tacggtgtt aattatttt 
ccgataatg aataaaaaa gagactgag ttgtggtta 
ggtgtaaca cctctagga ttaaacatc tacgaaaag 
gaaaataaa ctacaacca aaaacgacg ttcacatgg 
gccgaaata cgacacatt agttttgac gataaaaaa 
tttattatc aaaccggtc gataagaat tccccaaat 
tttgtcttc tttagccaa aaagttaga atgaataaa 
ttgattctt gatttgtgc atgggtaac catgattta 
ttcatgcgg aggcgtaaa cccgattct atggaactc 
caacaaatg aaagccgcc gccaaagaa gagaaacaa 
cgtcggcag attgaacga aatcgtctg gcacgtgaa 
aaacaattg agagaggag gcggagcgt gaaagggcc 
aatatggaa cagcgcctg ctccagtac caggaggaa 
atccgattg gctaatgaa gctctgaaa cgttccgaa 
gaaagcgcc gatttgctg gccgaaaaa agccgagtt 
gctgaagaa gaagcaatt cttctgagc cagaaagcg 
gccgaagcg gaacaagaa atcacccgt ttgcgcctc 
agcgcgatg cgaaaggaa gaagaaaaa gtcacattg 
gagcgaaaa actcgtgaa gcagaatta ttaacagct 
cgattagtt gaagattcg gaacgaaga gcggctgaa 
gcgaaccga ctgaaagaa gaattgttg cgggcgcga 
gctgctgaa aaacaagcc aaagaaaaa ttgttggat 
tttttgagt cgcggctct tattcgtcg tctgcaacg 
tcagtttcg accctatat tcggcctcg ttgagtcct 
ctggggccg gatttgtcg cttttggat ggcgatgtc 
gggacttcc ccggaactt aacctcacg ctgaatggt 
tacgatttg acgaacgcg gacaccgat caattgtcg 
ctcgaaatt gagaaggag agaggcttg tgtttggaa 
aaacagaag catttgcaa caccagcta cgcgagctt 
cgcaccgaa atcgctgtt ttgaaggtg gcggaaaaa 
cagaccgag tttgaccaa ctgcacagc gagcaagtg 
aaattaggc gaaaataaa tactcgacg ttgaaaaag 
agcaaatct gggtcgacg aagtcgcgg gtggccttc 
ttcgaagaa ctgtaa
    mRNA sequence
    • >TC014860 RA
      ctggggccc catcgcccg aaagtccta gtaatttcc 
cgccattgt gttggtatt ccgaattga catctgtca 
atgccataa ccgagatgt ttgtgcctt atttgttgt 
tgttatttt acggttgaa taatgcgat aggttttac 
aaaagcgag gattatgtt agaaaaaat gtctccatt 
tcgcaaaaa gaaatccga gaagagttt ccccgtgaa 
agtgtgcac attggacgc cgaattgga gttcaactt 
acaatggcg agccaccgg cagggattt attcgagct 
agtgtgccg cacaatcgg gttacgcga gacttggta 
tttcggttt gcaatacga agattcaaa agggtttat 
caattggtt aaaattgga caagaaagt ccaagacca 
gagcatcca gaaggaccc ccacagtac ggtgtcgtt 
catgttttt cgcgaaatt ctaccccga agaagtggc 
cgaagagct ggttcaaga ggtaacaaa acactattt 
ttttctcca agttaagca agctattct ctctatgga 
tgtgtactg cccccctga ggcgtccgt cctgctagc 
cagttacgc ggtccaagc caagtttgg agactttga 
cctagatac gtacaaacc gggaatgtt agcgaacga 
ggatttgtt gccccaaag agtcatcga ccagtatca 
gatgactct agacatgtg ggaggagcg gataagagt 
gtggtactc agatcacag gggtatgtc gcgagacga 
agccgagat ggagtattt aaaaatcgc acaagactt 
ggacatgta cggtgttaa ttattttcc gataatgaa 
taaaaaaga gactgagtt gtggttagg tgtaacacc 
tctaggatt aaacatcta cgaaaagga aaataaact 
acaaccaaa aacgacgtt cacatgggc cgaaatacg 
acacattag ttttgacga taaaaaatt tattatcaa 
accggtcga taagaattc cccaaattt tgtcttctt 
tagccaaaa agttagaat gaataaatt gattcttga 
tttgtgcat gggtaacca tgatttatt catgcggag 
gcgtaaacc cgattctat ggaactcca acaaatgaa 
agccgccgc caaagaaga gaaacaacg tcggcagat 
tgaacgaaa tcgtctggc acgtgaaaa acaattgag 
agaggaggc ggagcgtga aagggccaa tatggaaca 
gcgcctgct ccagtacca ggaggaaat ccgattggc 
taatgaagc tctgaaacg ttccgaaga aagcgccga 
tttgctggc cgaaaaaag ccgagttgc tgaagaaga 
agcaattct tctgagcca gaaagcggc cgaagcgga 
acaagaaat cacccgttt gcgcctcag cgcgatgcg 
aaaggaaga agaaaaagt cacattgga gcgaaaaac 
tcgtgaagc agaattatt aacagctcg attagttga 
agattcgga acgaagagc ggctgaagc gaaccgact 
gaaagaaga attgttgcg ggcgcgagc tgctgaaaa 
acaagccaa agaaaaatt gttggattt tttgagtcg 
cggctctta ttcgtcgtc tgcaacgtc agtttcgac 
cctatattc ggcctcgtt gagtcctct ggggccgga 
tttgtcgct tttggatgg cgatgtcgg gacttcccc 
ggaacttaa cctcacgct gaatggtta cgatttgac 
gaacgcgga caccgatca attgtcgct cgaaattga 
gaaggagag aggcttgtg tttggaaaa acagaagca 
tttgcaaca ccagctacg cgagcttcg caccgaaat 
cgctgtttt gaaggtggc ggaaaaaca gaccgagtt 
tgaccaact gcacagcga gcaagtgaa attaggcga 
aaataaata ctcgacgtt gaaaaagag caaatctgg 
gtcgacgaa gtcgcgggt ggccttctt cgaagaact 
gtaatttta ttattttta ataaaaatc tattatttt 
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    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY