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Gene information
General information
TC014856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9655955..9681292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014856
      iBeetle screen iB_05707

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014856 RA
        MDHKSQWHE RLFGQQLLK CDTNSPKSG DFLCVATPE 
        VLQNVQITG VYFSFANIS QQSDEFTKK LKVLYERLN 
        QENCEVKKF EVVQVVLWA NNDVFSDFE NSHRDSLVG 
        LPWFAVPFS EIDLKTRLS RRYRIKSGV PTLVLLDRD 
        GGTISVSAQ DRLLEDPLG SSFPWRPRP VDQVLKDVV 
        LQKGGTFSK EHPCTKPEF RYSDLPDAV RGFYFSANW 
        CPPCRAFTP QLAEVYRLI RKKEPGFEI VFVSSDRSA 
        ESFEAYVEG MPWLVVPWQ QAGVRAELA QLYGIRGIP 
        TLLLLDRNG HIITMDART ELAEDPMAQ NFPWKPRAV 
        NILTERFAN KLHDYPAIV LFVDGEETE LQFAESVLS 
        PAADSYYKK HNINFNSAP EDFFSNCED DHYLQFLIG 
        LDSETSDIL RDLIGLDDV VPLLVGIDI PNRRFAVME 
        YGVEITVDS VSDFVERFQ KGEVKFIEL NERVGEEQS
      CDS sequence
      • >TC014856 RA
        atggaccac aaaagccaa tggcacgag cgtctcttc 
        ggccaacaa cttctcaaa tgcgataca aattcgccg 
        aaatctggc gatttcctg tgcgtcgcg acgccggaa 
        gtcctacaa aacgttcaa ataaccggt gtttacttc 
        tcctttgct aatataagc caacaaagc gatgaattt 
        accaaaaaa ttaaaagtt ttgtacgaa agactcaac 
        caggaaaat tgcgaagtg aaaaaattc gaagtggtg 
        caagtggtt ttgtgggca aacaatgac gtgtttagt 
        gattttgaa aacagccac agggacagt cttgtggga 
        ctgccgtgg tttgcggta cccttcagt gagattgat 
        ttaaagaca cgcctctcc cgtcgctac cgcatcaaa 
        tcaggggtc ccaaccctc gtcctcctg gaccgtgac 
        gggggcacc atcagcgtg tcagcccag gaccgcctc 
        ctggaggac cccctcggc agttccttc ccatggcgc 
        ccgcgcccc gtcgaccag gtcctcaaa gacgtggtc 
        ttgcaaaaa gggggcacc ttctccaag gagcacccc 
        tgcaccaag cccgagttc cgctacagc gaccttccc 
        gacgccgtg cgagggttt tacttttcg gcgaactgg 
        tgcccccct tgtcgggcc ttcaccccg cagttggcc 
        gaagtgtat cgacttatt cggaagaag gagccgggc 
        tttgagatt gtcttcgtg agcagtgac agaagcgcc 
        gagtcattt gaggcttat gtggaaggc atgccctgg 
        ttggtggtg ccatggcaa caggcaggg gtcagggcc 
        gaactggcc caactgtac ggaatcagg ggaatcccc 
        acgttgcta cttttggac cgtaacgga catattatc 
        acaatggac gccaggacc gaactggcc gaagaccca 
        atggcacag aattttcca tggaaaccg cgcgcggtg 
        aatatttta accgagcgc ttcgctaat aaattacac 
        gattatccg gccatcgtc ttattcgtg gacggcgaa 
        gagaccgaa ctccagttc gcagaatcg gtcctgtcc 
        ccagccgcc gactcctac tacaagaag cacaacatc 
        aacttcaac tcagcccct gaggacttc ttctccaac 
        tgcgaggac gaccactac ctccagttc ctcatcggg 
        ctcgactcc gaaaccagc gacattctc cgcgacttg 
        atcggtctc gatgacgtg gtgcccctc ctcgttggc 
        atcgacatc cccaaccga cgcttcgcc gtcatggag 
        tacggcgtg gaaatcacc gtcgactcc gtcagcgac 
        ttcgtcgag cgcttccaa aaaggcgaa gtgaaattc 
        atcgaattg aacgaacga gtgggagag gaacagagt 
        tag
      mRNA sequence
      • >TC014856 RA
        gctctcgtc agtcgatgc gtccccgcc gccgcgacg 
        ctatgccat ggaccacaa aagccaatg gcacgagcg 
        tctcttcgg ccaacaact tctcaaatg cgatacaaa 
        ttcgccgaa atctggcga tttcctgtg cgtcgcgac 
        gccggaagt cctacaaaa cgttcaaat aaccggtgt 
        ttacttctc ctttgctaa tataagcca acaaagcga 
        tgaatttac caaaaaatt aaaagtttt gtacgaaag 
        actcaacca ggaaaattg cgaagtgaa aaaattcga 
        agtggtgca agtggtttt gtgggcaaa caatgacgt 
        gtttagtga ttttgaaaa cagccacag ggacagtct 
        tgtgggact gccgtggtt tgcggtacc cttcagtga 
        gattgattt aaagacacg cctctcccg tcgctaccg 
        catcaaatc aggggtccc aaccctcgt cctcctgga 
        ccgtgacgg gggcaccat cagcgtgtc agcccagga 
        ccgcctcct ggaggaccc cctcggcag ttccttccc 
        atggcgccc gcgccccgt cgaccaggt cctcaaaga 
        cgtggtctt gcaaaaagg gggcacctt ctccaagga 
        gcacccctg caccaagcc cgagttccg ctacagcga 
        ccttcccga cgccgtgcg agggtttta cttttcggc 
        gaactggtg ccccccttg tcgggcctt caccccgca 
        gttggccga agtgtatcg acttattcg gaagaagga 
        gccgggctt tgagattgt cttcgtgag cagtgacag 
        aagcgccga gtcatttga ggcttatgt ggaaggcat 
        gccctggtt ggtggtgcc atggcaaca ggcaggggt 
        cagggccga actggccca actgtacgg aatcagggg 
        aatccccac gttgctact tttggaccg taacggaca 
        tattatcac aatggacgc caggaccga actggccga 
        agacccaat ggcacagaa ttttccatg gaaaccgcg 
        cgcggtgaa tattttaac cgagcgctt cgctaataa 
        attacacga ttatccggc catcgtctt attcgtgga 
        cggcgaaga gaccgaact ccagttcgc agaatcggt 
        cctgtcccc agccgccga ctcctacta caagaagca 
        caacatcaa cttcaactc agcccctga ggacttctt 
        ctccaactg cgaggacga ccactacct ccagttcct 
        catcgggct cgactccga aaccagcga cattctccg 
        cgacttgat cggtctcga tgacgtggt gcccctcct 
        cgttggcat cgacatccc caaccgacg cttcgccgt 
        catggagta cggcgtgga aatcaccgt cgactccgt 
        cagcgactt cgtcgagcg cttccaaaa aggcgaagt 
        gaaattcat cgaattgaa cgaacgagt gggagagga 
        acagagtta ggtggtgta aggtgtagc agtgtagat 
        gagttaatc aaaagtgta cagtttgtt gttttgtag 
        catttaatt acctataaa atagtgtca attcacaca 
        attatatac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY