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Gene information
General information
TC031849
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014830
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451324.1: 2..1641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031849
      iBeetle screen iB_05705

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031849 RA
        MPGKRVSSM PLKKSSPKR FKAPGDGGC VEEEEISAS 
        QVPSVPKSS LSAFPAASP PPPPPSQSI ELARVSEIG 
        LSQAASPKS PQPIHAEED IITPAEWSD PVLERSLME 
        MADNIEKNE AERSEKDEA ERTARMVIN EKFRLIHEV 
        VYPITKSGS KLLSIGVKP LHDFVPLLK IHNPELTSA 
        ISFSIETFE EFLKEMSKI FESREEEDE EVEEISVPE 
        GQMLCMLSG YVVVVCRYN TLQFIPSQP SVYKGIYLS 
        LNTIKTVVN MGEHLLNLL HARRIQYKF YPCYDSFIN 
        NVALDVMEN GCKNLSQKL YDIIKNRYE QNATLYEIV 
        LKLPMNVKT AVEGRIQYY RENNDYNCL
      CDS sequence
      • >TC031849 RA
        atgcctgga aaacgtgta tcttctatg ccattgaaa 
        aaatcatct ccgaagcgt tttaaagcc ccaggtgat 
        ggtggatgt gtagaagaa gaggaaatt agtgcttcg 
        caagttcca tcggttccg aagtcgtct ttgtcagca 
        tttccagct gcttcacca ccaccacca ccaccatcg 
        caatccata gaattagcg agagtatct gaaataggt 
        cttagccaa gctgcatcc cccaagtcg cctcagcct 
        atccacgct gaagaagac atcataaca ccagctgaa 
        tggtcggat cctgtgttg gagcgatcg ttgatggaa 
        atggccgac aacatcgag aaaaatgaa gctgagaga 
        agtgagaaa gatgaagct gagagaacg gctcggatg 
        gtgatcaat gagaagttt cgtttaatt catgaagtg 
        gtgtatcct ataactaag tctggtagc aaactttta 
        tcgattgga gtaaaacca ctccatgat tttgtgccg 
        ttgttgaag atacataat ccagaatta acatcggcg 
        ataagtttt tctattgaa acctttgaa gagttttta 
        aaagaaatg agcaagatc tttgaatca agagaggag 
        gaggacgaa gaagtagaa gaaattagt gttccagaa 
        ggtcagatg ttgtgtatg ctaagtggc tacgtcgtg 
        gtggtttgt agatataat actcttcaa tttatacct 
        agtcaaccc agcgtgtac aagggcatt tatttatca 
        ttgaacaca attaaaact gtcgtaaat atgggcgag 
        catttattg aatctgctt catgcaaga agaattcaa 
        tataaattc tatccatgt tacgatagt tttataaat 
        aatgttgcg cttgatgtt atggagaat ggatgtaaa 
        aatttatct caaaaattg tatgatatt attaagaat 
        cggtatgaa caaaatgca accttgtat gagatagtt 
        ttgaaactg cctatgaat gtaaaaact gctgtcgaa 
        ggtagaata caatattat agggaaaat aatgattat 
        aactgtctg tga
      mRNA sequence
      • >TC031849 RA
        tcggaatta aacgataat ttattcgca ccaaattga 
        tgcctggaa aacgtgtat cttctatgc cattgaaaa 
        aatcatctc cgaagcgtt ttaaagccc caggtgatg 
        gtggatgtg tagaagaag aggaaatta gtgcttcgc 
        aagttccat cggttccga agtcgtctt tgtcagcat 
        ttccagctg cttcaccac caccaccac caccatcgc 
        aatccatag aattagcga gagtatctg aaataggtc 
        ttagccaag ctgcatccc ccaagtcgc ctcagccta 
        tccacgctg aagaagaca tcataacac cagctgaat 
        ggtcggatc ctgtgttgg agcgatcgt tgatggaaa 
        tggccgaca acatcgaga aaaatgaag ctgagagaa 
        gtgagaaag atgaagctg agagaacgg ctcggatgg 
        tgatcaatg agaagtttc gtttaattc atgaagtgg 
        tgtatccta taactaagt ctggtagca aacttttat 
        cgattggag taaaaccac tccatgatt ttgtgccgt 
        tgttgaaga tacataatc cagaattaa catcggcga 
        taagttttt ctattgaaa cctttgaag agtttttaa 
        aagaaatga gcaagatct ttgaatcaa gagaggagg 
        aggacgaag aagtagaag aaattagtg ttccagaag 
        gtcagatgt tgtgtatgc taagtggct acgtcgtgg 
        tggtttgta gatataata ctcttcaat ttataccta 
        gtcaaccca gcgtgtaca agggcattt atttatcat 
        tgaacacaa ttaaaactg tcgtaaata tgggcgagc 
        atttattga atctgcttc atgcaagaa gaattcaat 
        ataaattct atccatgtt acgatagtt ttataaata 
        atgttgcgc ttgatgtta tggagaatg gatgtaaaa 
        atttatctc aaaaattgt atgatatta ttaagaatc 
        ggtatgaac aaaatgcaa ccttgtatg agatagttt 
        tgaaactgc ctatgaatg taaaaactg ctgtcgaag 
        gtagaatac aatattata gggaaaata atgattata 
        actgtctgt gataataaa taattaaat aaaatgtat 
        actctgtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY