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Gene information
General information
TC031850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451324.1: 4855..10379
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC031850
      iBeetle screen iB_05704

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031850 RA
        MVVFVDFQG FNYGNCTET LTIKELAVF 
NTNKSAPDI FLFKPPDDL STLPHRYQK 
QADWLTNNF HGLSWNCGN YDYENMTRT 
GRGADDGAA ARKRARDGT GWRRRGNGR 
GTGWRRRGD GRDTHTHTH ILG
      CDS sequence
      • >TC031850 RA
        atggttgtg tttgtggat tttcaaggg ttcaattac 
ggtaattgt accgaaact ttaaccata aaagaatta 
gctgtattt aatacgaac aaaagcgca ccagacatc 
tttttgttt aaaccaccc gatgatctt tcaacctta 
ccgcatcgt tatcagaag caagccgat tggttgacg 
aataacttt cacgggctt tcttggaac tgcggtaat 
tacgactac gaaaacatg acgcggacg gggcgcggc 
gcggacgac ggggcggcg gcgcggaaa cgggcgcgg 
gacgggacg gggtggcgg cggcgcggg aacgggcgc 
gggacgggg tggcggcgg cgcggggac gggcgcgac 
acacacaca cacacacac attctgggt taa
      mRNA sequence
      • >TC031850 RA
        gtcttcgaa agaattcat gcgcgatct taaaatgaa 
aacgatcac atttgaaca cgatgtaat tatgacgaa 
atataagac taagcttga agctggtaa ctacgaaac 
gatcgaatc tattgtgga tgctctaaa ctcggtaag 
agttttagt aaaagataa tgttgagtt cagattaaa 
tgatggttc aaaaagagt aactgtaac aagcagtag 
caaatatct gacaagtct atcattttc accgacatt 
aagtctaca attaggttt tcaaccgga tacaaatgt 
actgagtaa aacttcaac acatccagc aaacattat 
gttgggttt gccttcaca acttttcgt atacagtga 
tataattga accacaatt ggtgggcga tataatggc 
aaaagtaat tcgaatcgt tgttatcga taataaaca 
ctacattta cggtacaca tcatacgca attattttc 
gcatccgca ttacgtgcc tctgttgaa aagggaatt 
tgaaaacat tgaaattga tataagaag tgcaactgg 
tgagaaagt gccatttca gtttggaac agtgtgcgt 
taaacttca ttttaaaaa aatttcgta atgccttca 
tgtcattat cacgattac tacatcaat caagctggt 
tcaggtgta ggtgctatt tacaaagga attagttac 
caaaagggt tatggtatt ggtagtttt ttacgggga 
ctttttcgt acggtaatg ccgttaata aagagtggt 
gttaaaaca attggtaag gaaacacta cgcactggt 
tcaaatttt cttggcgac attgttaat gaggtgcct 
gtaaaggat gcattcaag acacgtgta aatgagtca 
gttgaaaat ttgaaacga aaagctcaa gataaatta 
gacaacttg gtaggatct ggatctata aaaagaagg 
ctagtacaa aataaaagt cagaatagt tcttcacgt 
cgttctgca aagaagtgt aaaacgagt tcagttatt 
aaacatcaa gatatcttt agctagaat gtcgttttt 
acatcctca tagttcatt taacggtga ttattcctt 
aaatccatt taatttcga aaactttgg tttaaacta 
tctatcctt gtacgtgga tggtaacca aattcctag 
taaaccctt acaacctac atttatcgg tccaaataa 
gaaatttgt ttcaatgta ccacacatt attttctgg 
tactggtat acattttct aaacactgg aaatgggat 
ttcacgtga taattatgc tgatggata ttcaatagc 
tgtttttga tttgacacc tgacttatc atcgcataa 
tgctctttc atggaattt gataaagaa cggaagttt 
acgaataga agttggttt taacactgc tcttacaga 
aactgtcaa ttgtttagt gtatggtga atttgatag 
tgtaataga aattgataa aaaacgtaa tgtaattgt 
agattatag tagttgata aatttatat aagtgataa 
ataaattgc tattacaaa tgaaaaatt tgctacttt 
cattttacc cgtcatccg aaatggttg tgtttgtgg 
attttcaag ggttcaatt acggtaatt gtaccgaaa 
ctttaacca taaaagaat tagctgtat ttaatacga 
acaaaagcg caccagaca tctttttgt ttaaaccac 
ccgatgatc tttcaacct taccgcatc gttatcaga 
agcaagccg attggttga cgaataact ttcacgggc 
tttcttgga actgcggta attacgact acgaaaaca 
tgacgcgga cggggcgcg gcgcggacg acggggcgg 
cggcgcgga aacgggcgc gggacggga cggggtggc 
ggcggcgcg ggaacgggc gcgggacgg ggtggcggc 
ggcgcgggg acgggcgcg acacacaca cacacacac 
acattctgg gttaaatca aaataaaat cacagaaat 
catatttat tagatttat ttatttatt aaaagatta 
aataataca tcttatatt attatacat ctctatagc 
catatggta aggtgtcaa aagaaccat catctaaac 
gtcgtcgtt ttgtataat ttatacgat acgtcttag 
ttgtttctt ttgtcataa catcataag tctcatttc 
gaattattg tgcgatcgg tcaatttaa cagtttggt 
cggtgttat tacacagca tgtctttta ttttgtcga 
aattaacta attgagcat tttgataat gcaaactta 
accccttga ctttacaca cagtttgaa ttttgtgct 
cattttcat tccaaaatt tgtaggcat aatttttag 
gacccccac tgacaaatt cagttatat aactacctt 
cgccatatt caatcattt cgtcagtga ggtcaccta 
ggaaatcgc cagtaggag gttcatatt ccccttctt 
ttttaacga aaatgatac tatcggtat caaaataca 
atacccttt tgccaaggg gttttaaat acttatata 
attctaagc gtgcaccgg ctgttgtat agcatgcaa 
gagctacat ttaccgttt tcatgggcg ctatatctt 
cctctacac gttcccatg ttgccagaa taacgtctt 
cgttaatat gtaatagac tatttacaa ttgcagatt 
gatttgtta gcaacttaa acagctcat ggggatcgc 
taattatat cagtttttt taagatttt ccttttggc 
caaactttc cccaaaagc tgtttaaca ttattttag 
cgagaaatc tgagaccgg aattttttg atacgttgg 
ttggatcga gttttatac cttctcgtt cctcgtatt 
gttgaatat atacatttt tgtcttcat cggtactta 
cccaagatg gccaaccag aacactcga cctttaatt 
ttacgaatg cattaatgt attctgaaa agagtccgc 
ccgatttat taccccgat cgtaaattt ctacattgt 
attcccaaa tttcaataa gttccacta tactgtacc 
cttcttcaa tagctacac gtaattcat cggccacat 
atgtcccag taaattgcc gatcgacat cctcgtgaa 
aacaattgt cttgataat tagcttcag cgcaggtac 
gacataatg gaaatgtca attttccat tacatcgaa 
atggtaaca caggatggt aaagttgag caggaggta 
atacagtgc actttacta aaccttcga cagaattta 
ggtcgacat ttgcgcatt cctcttgcc caacataaa 
tctttttcg gatgcccaa ttggatatt tcccgtatt 
tattaatga atgggtata gggaacaaa catcctgat 
aagctattt tctcgtttt cattacatt tataatata 
atttacaag catttgttc ttcctccaa agaagccat 
cgcgtgggt ttaaaggag tgtgtttat tcaaataat 
ttcgattat caatgaaat tttttaatt ctgggttct 
ctttaattt ggtctttaa atatacatt ccctcattg 
taattaagt tatagccat atgttctaa tactctctt 
ctttacttt gtgtatttt cacgacgca agaataacg 
cgtcgtttt tatcggata atttctggg tttgattaa 
aaaaacata gctcgcacc cgtgccacc agcacccat 
ggaattcat aaacggtat tcgtctctt cacaatacc 
cgtcaacta gcataccct cttttaacc ggacttctc 
gcgcatttc cgctatgga taatatgaa cgttacttt 
ttttcgatt cccaaagta gccaattta tagcaatga 
cactttgtt tgtcagcgg ttctgtacc cagatactg 
gtatgatac cgattgaat tttcgcgta aatagttgc 
gtcggaata ctaaattgc atgctgagg c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY