Status
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Gene information
General information
TC031850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451324.1: 4855..10379
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031850
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05704

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031850 RA
        MVVFVDFQG FNYGNCTET LTIKELAVF 
        NTNKSAPDI FLFKPPDDL STLPHRYQK 
        QADWLTNNF HGLSWNCGN YDYENMTRT 
        GRGADDGAA ARKRARDGT GWRRRGNGR 
        GTGWRRRGD GRDTHTHTH ILG
      CDS sequence
      • >TC031850 RA
        atggttgtg tttgtggat tttcaaggg ttcaattac 
        ggtaattgt accgaaact ttaaccata aaagaatta 
        gctgtattt aatacgaac aaaagcgca ccagacatc 
        tttttgttt aaaccaccc gatgatctt tcaacctta 
        ccgcatcgt tatcagaag caagccgat tggttgacg 
        aataacttt cacgggctt tcttggaac tgcggtaat 
        tacgactac gaaaacatg acgcggacg gggcgcggc 
        gcggacgac ggggcggcg gcgcggaaa cgggcgcgg 
        gacgggacg gggtggcgg cggcgcggg aacgggcgc 
        gggacgggg tggcggcgg cgcggggac gggcgcgac 
        acacacaca cacacacac attctgggt taa
      mRNA sequence
      • >TC031850 RA
        gtcttcgaa agaattcat gcgcgatct taaaatgaa 
        aacgatcac atttgaaca cgatgtaat tatgacgaa 
        atataagac taagcttga agctggtaa ctacgaaac 
        gatcgaatc tattgtgga tgctctaaa ctcggtaag 
        agttttagt aaaagataa tgttgagtt cagattaaa 
        tgatggttc aaaaagagt aactgtaac aagcagtag 
        caaatatct gacaagtct atcattttc accgacatt 
        aagtctaca attaggttt tcaaccgga tacaaatgt 
        actgagtaa aacttcaac acatccagc aaacattat 
        gttgggttt gccttcaca acttttcgt atacagtga 
        tataattga accacaatt ggtgggcga tataatggc 
        aaaagtaat tcgaatcgt tgttatcga taataaaca 
        ctacattta cggtacaca tcatacgca attattttc 
        gcatccgca ttacgtgcc tctgttgaa aagggaatt 
        tgaaaacat tgaaattga tataagaag tgcaactgg 
        tgagaaagt gccatttca gtttggaac agtgtgcgt 
        taaacttca ttttaaaaa aatttcgta atgccttca 
        tgtcattat cacgattac tacatcaat caagctggt 
        tcaggtgta ggtgctatt tacaaagga attagttac 
        caaaagggt tatggtatt ggtagtttt ttacgggga 
        ctttttcgt acggtaatg ccgttaata aagagtggt 
        gttaaaaca attggtaag gaaacacta cgcactggt 
        tcaaatttt cttggcgac attgttaat gaggtgcct 
        gtaaaggat gcattcaag acacgtgta aatgagtca 
        gttgaaaat ttgaaacga aaagctcaa gataaatta 
        gacaacttg gtaggatct ggatctata aaaagaagg 
        ctagtacaa aataaaagt cagaatagt tcttcacgt 
        cgttctgca aagaagtgt aaaacgagt tcagttatt 
        aaacatcaa gatatcttt agctagaat gtcgttttt 
        acatcctca tagttcatt taacggtga ttattcctt 
        aaatccatt taatttcga aaactttgg tttaaacta 
        tctatcctt gtacgtgga tggtaacca aattcctag 
        taaaccctt acaacctac atttatcgg tccaaataa 
        gaaatttgt ttcaatgta ccacacatt attttctgg 
        tactggtat acattttct aaacactgg aaatgggat 
        ttcacgtga taattatgc tgatggata ttcaatagc 
        tgtttttga tttgacacc tgacttatc atcgcataa 
        tgctctttc atggaattt gataaagaa cggaagttt 
        acgaataga agttggttt taacactgc tcttacaga 
        aactgtcaa ttgtttagt gtatggtga atttgatag 
        tgtaataga aattgataa aaaacgtaa tgtaattgt 
        agattatag tagttgata aatttatat aagtgataa 
        ataaattgc tattacaaa tgaaaaatt tgctacttt 
        cattttacc cgtcatccg aaatggttg tgtttgtgg 
        attttcaag ggttcaatt acggtaatt gtaccgaaa 
        ctttaacca taaaagaat tagctgtat ttaatacga 
        acaaaagcg caccagaca tctttttgt ttaaaccac 
        ccgatgatc tttcaacct taccgcatc gttatcaga 
        agcaagccg attggttga cgaataact ttcacgggc 
        tttcttgga actgcggta attacgact acgaaaaca 
        tgacgcgga cggggcgcg gcgcggacg acggggcgg 
        cggcgcgga aacgggcgc gggacggga cggggtggc 
        ggcggcgcg ggaacgggc gcgggacgg ggtggcggc 
        ggcgcgggg acgggcgcg acacacaca cacacacac 
        acattctgg gttaaatca aaataaaat cacagaaat 
        catatttat tagatttat ttatttatt aaaagatta 
        aataataca tcttatatt attatacat ctctatagc 
        catatggta aggtgtcaa aagaaccat catctaaac 
        gtcgtcgtt ttgtataat ttatacgat acgtcttag 
        ttgtttctt ttgtcataa catcataag tctcatttc 
        gaattattg tgcgatcgg tcaatttaa cagtttggt 
        cggtgttat tacacagca tgtctttta ttttgtcga 
        aattaacta attgagcat tttgataat gcaaactta 
        accccttga ctttacaca cagtttgaa ttttgtgct 
        cattttcat tccaaaatt tgtaggcat aatttttag 
        gacccccac tgacaaatt cagttatat aactacctt 
        cgccatatt caatcattt cgtcagtga ggtcaccta 
        ggaaatcgc cagtaggag gttcatatt ccccttctt 
        ttttaacga aaatgatac tatcggtat caaaataca 
        atacccttt tgccaaggg gttttaaat acttatata 
        attctaagc gtgcaccgg ctgttgtat agcatgcaa 
        gagctacat ttaccgttt tcatgggcg ctatatctt 
        cctctacac gttcccatg ttgccagaa taacgtctt 
        cgttaatat gtaatagac tatttacaa ttgcagatt 
        gatttgtta gcaacttaa acagctcat ggggatcgc 
        taattatat cagtttttt taagatttt ccttttggc 
        caaactttc cccaaaagc tgtttaaca ttattttag 
        cgagaaatc tgagaccgg aattttttg atacgttgg 
        ttggatcga gttttatac cttctcgtt cctcgtatt 
        gttgaatat atacatttt tgtcttcat cggtactta 
        cccaagatg gccaaccag aacactcga cctttaatt 
        ttacgaatg cattaatgt attctgaaa agagtccgc 
        ccgatttat taccccgat cgtaaattt ctacattgt 
        attcccaaa tttcaataa gttccacta tactgtacc 
        cttcttcaa tagctacac gtaattcat cggccacat 
        atgtcccag taaattgcc gatcgacat cctcgtgaa 
        aacaattgt cttgataat tagcttcag cgcaggtac 
        gacataatg gaaatgtca attttccat tacatcgaa 
        atggtaaca caggatggt aaagttgag caggaggta 
        atacagtgc actttacta aaccttcga cagaattta 
        ggtcgacat ttgcgcatt cctcttgcc caacataaa 
        tctttttcg gatgcccaa ttggatatt tcccgtatt 
        tattaatga atgggtata gggaacaaa catcctgat 
        aagctattt tctcgtttt cattacatt tataatata 
        atttacaag catttgttc ttcctccaa agaagccat 
        cgcgtgggt ttaaaggag tgtgtttat tcaaataat 
        ttcgattat caatgaaat tttttaatt ctgggttct 
        ctttaattt ggtctttaa atatacatt ccctcattg 
        taattaagt tatagccat atgttctaa tactctctt 
        ctttacttt gtgtatttt cacgacgca agaataacg 
        cgtcgtttt tatcggata atttctggg tttgattaa 
        aaaaacata gctcgcacc cgtgccacc agcacccat 
        ggaattcat aaacggtat tcgtctctt cacaatacc 
        cgtcaacta gcataccct cttttaacc ggacttctc 
        gcgcatttc cgctatgga taatatgaa cgttacttt 
        ttttcgatt cccaaagta gccaattta tagcaatga 
        cactttgtt tgtcagcgg ttctgtacc cagatactg 
        gtatgatac cgattgaat tttcgcgta aatagttgc 
        gtcggaata ctaaattgc atgctgagg c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY