| General information | |||
| TC031850 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451324.1: 4855..10379 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031850 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05704 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC031850 RA
MVVFVDFQG FNYGNCTET LTIKELAVF NTNKSAPDI FLFKPPDDL STLPHRYQK QADWLTNNF HGLSWNCGN YDYENMTRT GRGADDGAA ARKRARDGT GWRRRGNGR GTGWRRRGD GRDTHTHTH ILG
- >TC031850 RA
atggttgtg tttgtggat tttcaaggg ttcaattac ggtaattgt accgaaact ttaaccata aaagaatta gctgtattt aatacgaac aaaagcgca ccagacatc tttttgttt aaaccaccc gatgatctt tcaacctta ccgcatcgt tatcagaag caagccgat tggttgacg aataacttt cacgggctt tcttggaac tgcggtaat tacgactac gaaaacatg acgcggacg gggcgcggc gcggacgac ggggcggcg gcgcggaaa cgggcgcgg gacgggacg gggtggcgg cggcgcggg aacgggcgc gggacgggg tggcggcgg cgcggggac gggcgcgac acacacaca cacacacac attctgggt taa
- >TC031850 RA
gtcttcgaa agaattcat gcgcgatct taaaatgaa aacgatcac atttgaaca cgatgtaat tatgacgaa atataagac taagcttga agctggtaa ctacgaaac gatcgaatc tattgtgga tgctctaaa ctcggtaag agttttagt aaaagataa tgttgagtt cagattaaa tgatggttc aaaaagagt aactgtaac aagcagtag caaatatct gacaagtct atcattttc accgacatt aagtctaca attaggttt tcaaccgga tacaaatgt actgagtaa aacttcaac acatccagc aaacattat gttgggttt gccttcaca acttttcgt atacagtga tataattga accacaatt ggtgggcga tataatggc aaaagtaat tcgaatcgt tgttatcga taataaaca ctacattta cggtacaca tcatacgca attattttc gcatccgca ttacgtgcc tctgttgaa aagggaatt tgaaaacat tgaaattga tataagaag tgcaactgg tgagaaagt gccatttca gtttggaac agtgtgcgt taaacttca ttttaaaaa aatttcgta atgccttca tgtcattat cacgattac tacatcaat caagctggt tcaggtgta ggtgctatt tacaaagga attagttac caaaagggt tatggtatt ggtagtttt ttacgggga ctttttcgt acggtaatg ccgttaata aagagtggt gttaaaaca attggtaag gaaacacta cgcactggt tcaaatttt cttggcgac attgttaat gaggtgcct gtaaaggat gcattcaag acacgtgta aatgagtca gttgaaaat ttgaaacga aaagctcaa gataaatta gacaacttg gtaggatct ggatctata aaaagaagg ctagtacaa aataaaagt cagaatagt tcttcacgt cgttctgca aagaagtgt aaaacgagt tcagttatt aaacatcaa gatatcttt agctagaat gtcgttttt acatcctca tagttcatt taacggtga ttattcctt aaatccatt taatttcga aaactttgg tttaaacta tctatcctt gtacgtgga tggtaacca aattcctag taaaccctt acaacctac atttatcgg tccaaataa gaaatttgt ttcaatgta ccacacatt attttctgg tactggtat acattttct aaacactgg aaatgggat ttcacgtga taattatgc tgatggata ttcaatagc tgtttttga tttgacacc tgacttatc atcgcataa tgctctttc atggaattt gataaagaa cggaagttt acgaataga agttggttt taacactgc tcttacaga aactgtcaa ttgtttagt gtatggtga atttgatag tgtaataga aattgataa aaaacgtaa tgtaattgt agattatag tagttgata aatttatat aagtgataa ataaattgc tattacaaa tgaaaaatt tgctacttt cattttacc cgtcatccg aaatggttg tgtttgtgg attttcaag ggttcaatt acggtaatt gtaccgaaa ctttaacca taaaagaat tagctgtat ttaatacga acaaaagcg caccagaca tctttttgt ttaaaccac ccgatgatc tttcaacct taccgcatc gttatcaga agcaagccg attggttga cgaataact ttcacgggc tttcttgga actgcggta attacgact acgaaaaca tgacgcgga cggggcgcg gcgcggacg acggggcgg cggcgcgga aacgggcgc gggacggga cggggtggc ggcggcgcg ggaacgggc gcgggacgg ggtggcggc ggcgcgggg acgggcgcg acacacaca cacacacac acattctgg gttaaatca aaataaaat cacagaaat catatttat tagatttat ttatttatt aaaagatta aataataca tcttatatt attatacat ctctatagc catatggta aggtgtcaa aagaaccat catctaaac gtcgtcgtt ttgtataat ttatacgat acgtcttag ttgtttctt ttgtcataa catcataag tctcatttc gaattattg tgcgatcgg tcaatttaa cagtttggt cggtgttat tacacagca tgtctttta ttttgtcga aattaacta attgagcat tttgataat gcaaactta accccttga ctttacaca cagtttgaa ttttgtgct cattttcat tccaaaatt tgtaggcat aatttttag gacccccac tgacaaatt cagttatat aactacctt cgccatatt caatcattt cgtcagtga ggtcaccta ggaaatcgc cagtaggag gttcatatt ccccttctt ttttaacga aaatgatac tatcggtat caaaataca atacccttt tgccaaggg gttttaaat acttatata attctaagc gtgcaccgg ctgttgtat agcatgcaa gagctacat ttaccgttt tcatgggcg ctatatctt cctctacac gttcccatg ttgccagaa taacgtctt cgttaatat gtaatagac tatttacaa ttgcagatt gatttgtta gcaacttaa acagctcat ggggatcgc taattatat cagtttttt taagatttt ccttttggc caaactttc cccaaaagc tgtttaaca ttattttag cgagaaatc tgagaccgg aattttttg atacgttgg ttggatcga gttttatac cttctcgtt cctcgtatt gttgaatat atacatttt tgtcttcat cggtactta cccaagatg gccaaccag aacactcga cctttaatt ttacgaatg cattaatgt attctgaaa agagtccgc ccgatttat taccccgat cgtaaattt ctacattgt attcccaaa tttcaataa gttccacta tactgtacc cttcttcaa tagctacac gtaattcat cggccacat atgtcccag taaattgcc gatcgacat cctcgtgaa aacaattgt cttgataat tagcttcag cgcaggtac gacataatg gaaatgtca attttccat tacatcgaa atggtaaca caggatggt aaagttgag caggaggta atacagtgc actttacta aaccttcga cagaattta ggtcgacat ttgcgcatt cctcttgcc caacataaa tctttttcg gatgcccaa ttggatatt tcccgtatt tattaatga atgggtata gggaacaaa catcctgat aagctattt tctcgtttt cattacatt tataatata atttacaag catttgttc ttcctccaa agaagccat cgcgtgggt ttaaaggag tgtgtttat tcaaataat ttcgattat caatgaaat tttttaatt ctgggttct ctttaattt ggtctttaa atatacatt ccctcattg taattaagt tatagccat atgttctaa tactctctt ctttacttt gtgtatttt cacgacgca agaataacg cgtcgtttt tatcggata atttctggg tttgattaa aaaaacata gctcgcacc cgtgccacc agcacccat ggaattcat aaacggtat tcgtctctt cacaatacc cgtcaacta gcataccct cttttaacc ggacttctc gcgcatttc cgctatgga taatatgaa cgttacttt ttttcgatt cccaaagta gccaattta tagcaatga cactttgtt tgtcagcgg ttctgtacc cagatactg gtatgatac cgattgaat tttcgcgta aatagttgc gtcggaata ctaaattgc atgctgagg c
