Status
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Gene information
General information
TC014819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450245.1: 4755..35871
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014819
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05701

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014819 RA
      MTEGSDECS KGACSTAAK ETSRRLNAL TKTEIPENK 
      LKKEEFRPR IRWPDLTVQ VFIHVGCLY GLYLCLVSA 
      RLYTTLFAF LTIYLSGFG ITAGAHRLW SHRAYKAKW 
      PLKILLLIL FTITGQRHV YVWALDHRV HHKYSETDA 
      DPHNAKRGF LFSHVGWLV LTPHPDVVE KRKAVDMSD 
      LEADPLIMW HKKLYPPLF FLFVIFLPV FTPVYFWNE 
      TIWNSFWVN FNARFCITL NIAFFVNSV AHMWGQKPY 
      DRYISPVEN LAVSLAALG EGWHNYHHV FPWDYRTGE 
      LGNSYNPST TFIDFFAKI GWAYDLKFA SNSMIARRA 
      KKSGDGSHI WGYGDADIE KEDLEELNK MEDFN
    CDS sequence
    • >TC014819 RA
      atgacggaa ggcagcgat gaatgttca aaaggtgct 
      tgttcaact gctgctaaa gagacatcg cgacgtctg 
      aacgcttta accaaaacc gaaattcca gaaaacaaa 
      ttgaaaaaa gaagaattc cggccaaga attcgatgg 
      cctgattta acagtacaa gtatttatt catgtagga 
      tgtttatat ggactgtat ttgtgcctt gtgtctgcc 
      agattatac acaacactt tttgcattc ctaacgata 
      tacctctca gggtttggc atcaccgcg ggtgctcat 
      agattatgg tcacatagg gcttacaag gccaaatgg 
      ccacttaag atacttcta ctcatctta tttacgatt 
      acgggtcag aggcatgtc tacgtctgg gcattggac 
      catcgagtt caccacaaa tacagcgaa accgacgcc 
      gatccacat aatgcgaaa cgcggtttt ctgttttca 
      cacgtcggt tggcttgtt ctaacccct catcctgat 
      gttgtcgaa aaacgaaaa gccgtcgat atgagcgac 
      ttggaagcc gatccgctc ataatgtgg cacaaaaaa 
      ttatatcca ccactattt ttcctgttc gttattttt 
      ctaccagtt tttaccccc gtgtatttt tggaacgaa 
      acaatttgg aattcgttt tgggtgaat tttaacgcc 
      agattttgt atcacttta aacatcgca tttttcgtg 
      aacagcgtt gcgcatatg tggggccag aaaccttac 
      gacagatat ataagtcca gtggaaaat ttagcagtg 
      tctttggca gcgttgggt gaaggttgg cataactac 
      catcacgtc ttcccatgg gattacaga actggagaa 
      cttggaaat agctacaac ccatctacc acgtttatt 
      gatttcttc gcaaaaatt ggatgggct tacgacttg 
      aaattcgct tcgaattcg atgattgca agaagagcg 
      aagaaaagt ggcgatgga agtcacatt tggggttac 
      ggagatgca gatatagaa aaagaagat ttagaagaa 
      ctgaataaa atggaggat tttaactga
    mRNA sequence
    • >TC014819 RA
      tgatatttt aatattttt attttttat tttaattat 
      ttttttatt gtttttatt tttaatatg atatttttt 
      acaattttt ctagaagct gcaaaccgt cttgatttt 
      caatccatc gaaacgaaa tgctttgaa tcgcagaaa 
      acaaacgct ccacttcct gcttcactt gaacttgga 
      accactcct tccggttct tccataggc aactttcaa 
      caattacat catatatct acaggttgt tgtcgaagg 
      cgtggtggc gcacgtctt tccccgcaa gcaccaaaa 
      catttcaaa cgcaggcga gcagtagat cgcagttgg 
      cgttgaatg tgtttgtgt tacgtttgc gtaaagttc 
      agtgattac cacgtggcg gcaggcgag ccttcggat 
      ctgctgtca acaggagat acggcgacc gatcgcagg 
      aaaattttg ccgttatct ctgaagcga gatttagtg 
      aatgaacga acgttgaat gtgagatgt gcaccactt 
      ttagtaagt gaagtggtc ttgcaattt tgactgttg 
      caaaacaaa aatgacgga aggcagcga tgaatgttc 
      aaaaggtgc ttgttcaac tgctgctaa agagacatc 
      gcgacgtct gaacgcttt aaccaaaac cgaaattcc 
      agaaaacaa attgaaaaa agaagaatt ccggccaag 
      aattcgatg gcctgattt aacagtaca agtatttat 
      tcatgtagg atgtttata tggactgta tttgtgcct 
      tgtgtctgc cagattata cacaacact ttttgcatt 
      cctaacgat atacctctc agggtttgg catcaccgc 
      gggtgctca tagattatg gtcacatag ggcttacaa 
      ggccaaatg gccacttaa gatacttct actcatctt 
      atttacgat tacgggtca gaggcatgt ctacgtctg 
      ggcattgga ccatcgagt tcaccacaa atacagcga 
      aaccgacgc cgatccaca taatgcgaa acgcggttt 
      tctgttttc acacgtcgg ttggcttgt tctaacccc 
      tcatcctga tgttgtcga aaaacgaaa agccgtcga 
      tatgagcga cttggaagc cgatccgct cataatgtg 
      gcacaaaaa attatatcc accactatt tttcctgtt 
      cgttatttt tctaccagt ttttacccc cgtgtattt 
      ttggaacga aacaatttg gaattcgtt ttgggtgaa 
      ttttaacgc cagattttg tatcacttt aaacatcgc 
      atttttcgt gaacagcgt tgcgcatat gtggggcca 
      gaaacctta cgacagata tataagtcc agtggaaaa 
      tttagcagt gtctttggc agcgttggg tgaaggttg 
      gcataacta ccatcacgt cttcccatg ggattacag 
      aactggaga acttggaaa tagctacaa cccatctac 
      cacgtttat tgatttctt cgcaaaaat tggatgggc 
      ttacgactt gaaattcgc ttcgaattc gatgattgc 
      aagaagagc gaagaaaag tggcgatgg aagtcacat 
      ttggggtta cggagatgc agatataga aaaagaaga 
      tttagaaga actgaataa aatggagga ttttaactg 
      attaaagtc aatgccagt aaacagtat tagccagta 
      taaaataat ccctagagt ttttgtcag ttgcttttt 
      tagctgaat ttttagtgc tgttctttt ttgaaaaat 
      ttacaaagc caaacggtg ccatttagg cagcaatgc 
      ttttgaaag ttttagttt tgagtcaca aagccaaca 
      ataaagttt acttactta cttaaacat tcttgaacc 
      ggtttttta aatccagtt caacttaaa atgttttgt 
      gactttatt ttacttaca gccacagtt gtatggtgg 
      ggttgattc aaggaaatc ttgcaatga aatcggaat 
      tagaataag tgtttcctt gaatgcgcc agtgactta 
      cataatttg tttttgttt tcttattag atttatggt 
      gttttttgt taagtctga acataccta tataatata 
      ataaaaaag tttgaaaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY