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Gene information
General information
TC014813 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15394508..15406241
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014813
    iBeetle screen iB_05699

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014813 RA
      MFWLRLVCS VLFFLAEGS WSLKSVRIR VPEVVKSGE 
      TVTLSCEYD LEEVALYTI KWYWKDVEF YRFVPKESP 
      PFRAFTMKY INVDISRSG PTQVTLRGV RRELTGDYK 
      CEVSADGPL FHTDIKVAH MIVAELPNG NPMMNIEQT 
      KVEIGKKIK ADCYSPGSD PAANLTWFI NDEQVSEET 
      DSVKLHPIE IETDNALGL HSAKSRIEI TTNRAHFIL 
      GFMVVKCDA RIYSLWHRV VEDHVRDDT PQLAPVLGS 
      TSSQSQVDE IIDGNGSGH LGFSALCVV NFLLVMVVL 
      R
    CDS sequence
    • >TC014813 RA
      atgttctgg ctgcgattg gtttgttcg gtcttgttc 
      ttcttagct gaaggatcc tggtcgttg aaatcggtg 
      aggatccga gtgccggaa gtagtcaaa tctggtgaa 
      acggtgacg ttatcgtgc gaatacgat ctcgaagag 
      gtagctctg tacacaatc aaatggtat tggaaagat 
      gtggagttc taccgcttc gtccctaaa gagtctcca 
      ccgtttcga gcgttcaca atgaaatac attaacgtc 
      gatatatcc cgctccggc ccgacacag gtaaccctc 
      cgtggggtt cgtcgggag ctaaccggc gactacaag 
      tgcgaagtc tcagccgat gggccccta tttcacacc 
      gatatcaaa gtggcacac atgatagtc gcagagctg 
      cccaacggt aatcccatg atgaatatc gaacagacc 
      aaggtggag attggcaag aagataaag gcagattgc 
      tactcgcct gggtccgat ccggccgcc aatctcacc 
      tggtttatc aacgacgaa caggttagt gaagaaacc 
      gacagtgtg aaactccac cccatcgaa atcgaaact 
      gacaatgct ctaggtctg cattcagcc aaatctcga 
      atcgagata acgacaaat agggcacat tttattttg 
      gggtttatg gttgtaaaa tgcgacgcg cgaatttat 
      tcactctgg catagggtt gttgaagat catgtcaga 
      gacgatacg ccccaatta gcaccggtt ttgggatcg 
      acgtcgtcg cagagtcaa gttgacgag ataatagat 
      gggaatgga agtggacat ctgggtttc agtgctctg 
      tgtgtggta aactttctt ttagtaatg gtagtgtta 
      agataa
    mRNA sequence
    • >TC014813 RA
      acttaaaac ttagctgtg tgtattttt attgtgctc 
      gacgatgtt ctggctgcg attggtttg ttcggtctt 
      gttcttctt agctgaagg atcctggtc gttgaaatc 
      ggtgaggat ccgagtgcc ggaagtagt caaatctgg 
      tgaaacggt gacgttatc gtgcgaata cgatctcga 
      agaggtagc tctgtacac aatcaaatg gtattggaa 
      agatgtgga gttctaccg cttcgtccc taaagagtc 
      tccaccgtt tcgagcgtt cacaatgaa atacattaa 
      cgtcgatat atcccgctc cggcccgac acaggtaac 
      cctccgtgg ggttcgtcg ggagctaac cggcgacta 
      caagtgcga agtctcagc cgatgggcc cctatttca 
      caccgatat caaagtggc acacatgat agtcgcaga 
      gctgcccaa cggtaatcc catgatgaa tatcgaaca 
      gaccaaggt ggagattgg caagaagat aaaggcaga 
      ttgctactc gcctgggtc cgatccggc cgccaatct 
      cacctggtt tatcaacga cgaacaggt tagtgaaga 
      aaccgacag tgtgaaact ccaccccat cgaaatcga 
      aactgacaa tgctctagg tctgcattc agccaaatc 
      tcgaatcga gataacgac aaatagggc acattttat 
      tttggggtt tatggttgt aaaatgcga cgcgcgaat 
      ttattcact ctggcatag ggttgttga agatcatgt 
      cagagacga tacgcccca attagcacc ggttttggg 
      atcgacgtc gtcgcagag tcaagttga cgagataat 
      agatgggaa tggaagtgg acatctggg tttcagtgc 
      tctgtgtgt ggtaaactt tcttttagt aatggtagt 
      gttaagata atgaaatct tgattggta aaaagtgaa 
      gcgtgccta aattttgtt tattctttt acgatttta 
      tacaaattt tcagtctga gctggggtt agattggaa 
      atttgtgac tcgttgatt attaaatta ttgtggttc 
      cttttatga ttgttgttt tatacccat gatggcaat 
      aaaatgacg gaggaatag aactgtaat atattctgt 
      atacatacg agtaaatct gatactttt agtatgtac 
      atgtacaaa atgtgatac tatttagtc agtaaggcc 
      atgttattg ttatagtgg tagagtttt taatttatg 
      aacgaaaga ctaactaaa acttttata gcaattttc 
      cgatggtcg tgcaactaa gtgtttaca tgaagcgct 
      tcaaagtca taatttcgt ttgtgtaat aagtgtaga 
      tgttgagtt tgttctagg taataaagt tttgaaccg 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY