| General information | |||
| TC014806 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 15327983..15339943 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014806 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05698 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
| Comment: |
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
- >TC014806 RA
MSVPSMVHR SGEPLTTTA LFDDFDDEK DLPECKVKR NYSCASCDF FTQNPRHYL YHLRDVHEE KVRIYECPN CLYASKHFQ KLLRHTKMV HGDKDDDDR ADTPPPDDP ETVFKCSVC AFSARTRAQ LDRHERAEH IKTKFFRCT KCTYATHMK ARFTKHVKY HSMPMIKCD MCDFRTPYK WNLDRHCKN HNGKGAFRC SACNFTADI KQSLTVHEM NHHVPPVGQ AAGLGVGRR RNKVGASDA TAAEEAARQ EQQVPSEVE KKVKHSPEQ GSFGPDFIN PDDIIHHAN GNVYIKNKC KLCNFKTAW ASEMTRHEQ KVHNFNPEP KPKKPTRPI PNLIPIPSQ TSVLKLPKK NKEQEPEMS EQDINNICA KSANSALKD FASLFDSED VFENCENKI PDLIPTTKQ TTDEFKQKN ASFFDKLKE KLMSSNGTN SMTCQLCGY QSKCLSEQL KHQKKCGKE SNHPPSLTT FTSSSRCQY CRQRCKSSQ GLYTHQLTC PEYLKAAEE SENELRIDE SQSEDGENN NKPHPMENR VFIWNNIEF PMDVEVDDS KYEYKVDDN VSLDLSVRT QSPGGSERS ESSYVGAQE FATTHPVTD KIPTHGNDI TVAQHKRVF KCPHCTFWA STASRFHVH IVGHLNKKP FECSLCAYR SNWRWDITK HIKLKSVRD PAHESAKVL MTDETGRRN YTKYNKYLT EIHVSGNQS LDTSGGSGT RPKHHDRNT SPTQNKGDL PKLTRAPSG NDFSPLLRP PPPLKAADG SFFKVDKNK RNNMDNKRT FFKCKKCNF RDASREILL AHVKGHYPQ GLSTPSPGQ DLSMRGSSP EKDEASVST SAKGSQAPF RCGHCNQVS NWKHVIQRH CRLKHSGDI RVVSNKNGQ EKLEIEEQT DEPDHVDVP QDGAFKCNE CPFSSDSQE ELEEHLPGH TATNESVSK CQFCNYFVN QKESLFDHL RLHGIADPE EFLSKFYSS PEGDLGKRF KCMVCPYVT NSKSQYTYH KQFHKPRGG QYTCTQCSY NVSKRHLLH QHLKVHGIS ITPQKQNGE VIDLDDISD EIEEVSSGG FEGQNFPDI PLVWVSKND KFSKMFKCR YCPHVNLRK VNIQEHEKM HSVREKNPN SSRLNDVEH RCTECNYVC NNAGVLSSH SKVHQGLYG TVHCLVDQV KSDEEQIRE LSKFLSPRP LETINLDDY ENPPECSGF GGDLDEQDE ANFLYFCGE CPARFLKEN EYKIHTRLH GLRSYYKCD HCSYSARQK PHLLAHKNV HSQQYQDRT QVLQSMYKS NNQPPDLYT CLNNEGETI WLVSGCNSE VKDLEMDVE SLSAILSKP LKNSLNVPL SGTELFQQK QAQMLKEPS PPKASDPQF GSLMHGNPN FVYPTYLKN GKMKEKRYK CHKCPSAFE KREQYKVHL NLHGSKQRY NCEFCDYSV KYYANYVQH LKKHKMNSE ALAARDVEM DVEDEEVTI EASDEKKLS LGDQQTLNI LEQRLLVNS DNKEEEEKK VVTFSCPNC PYVNHRKDA VDNHQKRHG SKSNYTCEH CDYSVPQVH FLRDHAKLH FVPNNKGQV DGYMYCDSF KLVGRKVDE EKEDVIFDE GAEEVTDKF NNNDGEKQF VNVDTGEVA ERGSSDKMD ES
- >TC014806 RB
MSVPSMVHR SGEPLTTTA LFDDFDDEK DLPECKVKR NYSCASCDF FTQNPRHYL YHLRDVHEE KVRIYECPN CLYASKHFQ KLLRHTKMV HGDKDDDDR ADTPPPDDP ETVFKCSVC AFSARTRAQ LDRHERAEH IKTKFFRCT KCTYATHMK ARFTKHVKY HSMPMIKCD MCDFRTPYK WNLDRHCKN HNGKGAFRC SACNFTADI KQSLTVHEM NHHVPPVGQ AAGLGVGRR RNKVGASDA TAAEEAARQ EQQVPSEVE KKVKHSPEQ GSFGPDFIN PDDIIHHAN GNVYIKNKC KLCNFKTAW ASEMTRHEQ KVHNFNPEP KPKKPTRPI PNLIPIPSQ TSVLKLPKK NKEQEPEMS EQDINNICA KSANSALKD FASLFDSED VFENCENKI PDLIPTTKQ TTDEFKQKN ASFFDKLKE KLMSSNGTN SMTCQLCGY QSKCLSEQL KHQKKCGKE SNHPPSLTT FTSSSRCQY CRQRCKSSQ GLYTHQLTC PEYLKAAEE SENELRIDE SQSEDGENN NKPHPMENR VFIWNNIEF PMDVEVDDS KYEYKVDDN VSLDLSVRT QSPGGSERS ESSYVGAQE FATTHPVTD KIPTHGNDI TVAQHKRVF KCPHCTFWA STASRFHVH IVGHLNKKP FECSLCAYR SNWRWDITK HIKLKSVRD PAHESAKVL MTDETGRRN YTKYNKYLT EIHVSGNQS LDTSGGSGT RPKHHDRNT SPTQNKGDL PKLTRAPSG NDFSPLLRP PPPLKAADG SFFKVDKNK RNNMDNKRT FFKCKKCNF RDASREILL AHVKGHYPQ GLSTPSPGQ DLSMRGSSP EKDERHCRL KHSGDIRVV SNKNGQEKL EIEEQTDEP DHVDVPQDG AFKCNECPF SSDSQEELE EHLPGHTAT NESVSKCQF CNYFVNQKE SLFDHLRLH GIADPEEFL SKFYSSPEG DLGKRFKCM VCPYVTNSK SQYTYHKQF HKPRGGQYT CTQCSYNVS KRHLLHQHL KVHGISITP QKQNGEVID LDDISDEIE EVSSGGFEG QNFPDIPLV WVSKNDKFS KMFKCRYCP HVNLRKVNI QEHEKMHSV REKNPNSSR LNDVEHRCT ECNYVCNNA GVLSSHSKV HQGLYGTVH CLVDQVKSD EEQIRELSK FLSPRPLET INLDDYENP PECSGFGGD LDEQDEANF LYFCGECPA RFLKENEYK IHTRLHGLR SYYKCDHCS YSARQKPHL LAHKNVHSQ QYQDRTQVL QSMYKSNNQ PPDLYTCLN NEGETIWLV SGCNSEVKD LEMDVESLS AILSKPLKN SLNVPLSGT ELFQQKQAQ MLKEPSPPK ASDPQFGSL MHGNPNFVY PTYLKNGKM KEKRYKCHK CPSAFEKRE QYKVHLNLH GSKQRYNCE FCDYSVKYY ANYVQHLKK HKMNSEALA ARDVEMDVE DEEVTIEAS DEKKLSLGD QQTLNILEQ RLLVNSDNK EEEEKKVVT FSCPNCPYV NHRKDAVDN HQKRHGSKS NYTCEHCDY SVPQVHFLR DHAKLHFVP NNKGQVDGY MYCDSFKLV GRKVDEEKE DVIFDEGAE EVTDKFNNN DGEKQFVNV DTGEVAERG SSDKMDES
- >TC014806 RA
atgtccgtg ccgtcgatg gtacatcga tcaggagag cctctcacg acgactgcg cttttcgac gactttgac gacgaaaaa gacctcccc gagtgcaag gtgaagcga aactactcg tgcgccagc tgtgacttc ttcacgcaa aaccccagg cactacctc taccacctc cgcgatgtc cacgaggag aaagtgagg atctacgag tgccccaac tgtctctac gcctccaaa cacttccag aagctgcta aggcacacc aagatggtg cacggcgac aaggacgac gacgacagg gcggacacc ccgcccccg gacgacccc gaaacggtg ttcaagtgc tcggtgtgc gcgtttagc gcccgcacc cgcgcgcag ctcgaccgt cacgagcgc gccgaacac atcaagaca aagttcttc aggtgcacc aagtgcacg tacgccacc cacatgaag gcgcgtttc accaaacac gtgaaatac cactcgatg ccgatgatc aagtgcgac atgtgcgac ttccgcacc ccttacaag tggaacctg gaccgccac tgcaagaac cacaacggc aaaggtgcg ttccgatgc tcggcgtgc aacttcacc gccgacatc aagcaaagt ctcaccgtg cacgagatg aaccaccat gtgcctccc gtgggccag gcggccggt ttgggggtg ggacgccga cgcaacaag gtgggggcg agcgacgcc accgccgcc gaggaggcc gcacgccaa gaacaacag gtgccttct gaagtggaa aagaaagtg aagcacagc cctgagcaa gggtccttc gggccggat tttatcaac ccggatgat atcatccat catgccaat ggcaacgtt tatatcaaa aataagtgc aaactgtgc aatttcaag acggcttgg gcctcggaa atgacccgt cacgagcaa aaagtccac aactttaac cctgagccc aaaccgaaa aaaccgaca agacccata cccaacctc atccctata ccttcacaa acttcggtg ttaaaactt cccaagaag aataaggaa caagaaccg gaaatgagc gagcaagac attaacaat atttgtgcg aaatcggcc aatagtgcc ctcaaagac tttgcttcg ttgttcgac agtgaagat gttttcgaa aattgcgaa aacaaaatc cctgatctc atcccaacc actaaacaa accaccgat gaatttaaa caaaaaaac gcctctttc ttcgataaa ctcaaggag aaactgatg agcagcaat gggactaac tctatgacg tgtcaattg tgcggctat cagagcaaa tgcttgtct gaacaactc aaacatcag aaaaagtgc ggcaaggag agcaaccac cctccaagc ttaaccacg ttcaccagc tcctccagg tgccagtat tgccgtcaa aggtgcaag tcaagtcaa ggactttac acccaccaa ttgacctgt cccgagtat ctcaaagcg gccgaagaa tccgaaaac gagcttcga attgacgaa agtcagtcc gaagacggt gaaaacaac aacaaaccc caccctatg gaaaatcgc gtctttatt tggaacaac atcgagttt ccaatggat gtcgaagtc gacgattcc aagtatgag tacaaagtg gacgacaac gtctccctg gacctctcc gtgcgcacc caatcacca ggaggtagc gaacgcagc gagagcagc tacgtgggg gcccaagaa ttcgccaca acccatccc gttaccgac aagatcccc acccatggg aacgacatc accgtggcg caacacaaa cgcgtgttc aagtgccca cattgcacc ttttgggca tcaacagcg tcacgtttc cacgtgcat atcgtcggt catttgaat aaaaaaccc tttgaatgt tcactgtgt gcgtacaga tcgaattgg cgatgggac atcaccaaa catattaaa ttgaaatcg gtcagagac ccagcacat gaaagcgcc aaagtttta atgacggac gaaacaggc cgaagaaat tacaccaag tataataaa tacttgacg gagatacat gtgagtgga aatcaaagt ttagatacg agtggtggg tcgggaaca aggccgaaa catcacgat cgcaatact tcgccgact cagaataaa ggggattta ccgaaattg acgcgagcg ccgtctggg aatgatttt agtccgttg ttgaggcct ccgcctcca ttgaaagcc gccgatgga tcgttcttc aaggttgat aagaataaa aggaacaat atggataat aagaggacg tttttcaaa tgcaagaag tgcaatttt agggacgcc tctcgcgag atcctcttg gcgcacgtg aagggccac tacccgcag ggcctttcg acgcccagt ccaggtcag gacttgagt atgcgaggt tccagtcca gagaaagac gaggcaagt gttagcacg tcagcgaaa gggagccaa gcgcccttc cgctgcggt cactgcaac caggtatca aactggaag catgtcatt cagcgtcac tgtcgcctg aaacacagc ggtgatata cgcgtcgtt agcaacaaa aacggtcaa gaaaaactc gaaattgaa gagcaaacc gacgaacct gaccatgtt gatgtaccc caagatggg gctttcaaa tgcaacgag tgccccttt tctagtgat agtcaagag gagctcgaa gaacacctt cctggccat acggcgacg aatgaaagt gtctccaag tgtcaattt tgcaattat ttcgtcaat caaaaagag agtcttttt gatcatttg cgactgcac ggaatcgca gatcctgaa gagttcctc agcaagttt tattcgagt cctgagggg gaccttggg aaaaggttc aagtgcatg gtgtgcccc tacgtgacc aactccaag tcgcagtac acctatcac aaacagttt cacaaaccg agagggggc caatacact tgcacccaa tgtagttac aatgtgagt aagcggcac ttgttgcac caacatttg aaagtgcat gggattagt attacgcct cagaagcaa aacggcgaa gtgatcgat cttgacgat atttccgac gaaatagag gaggtgtca tctggtggt ttcgaggga caaaacttc ccagatatc ccgctagtt tgggtctcg aaaaatgat aaattctcc aagatgttt aagtgtcgc tactgtcca catgtcaat ttacggaaa gttaacatc caagaacac gagaaaatg cacagtgtc agggagaaa aacccgaat tcgagtcgt ttgaacgat gtggaacac cgatgtacg gagtgtaac tacgtttgt aacaatgcc ggggtttta tcatcgcat tcaaaggtc caccaaggg ttgtacggg acggttcat tgtctcgta gatcaggtc aagtcagac gaggagcaa atccgcgaa ttgagcaag tttttaagt ccacgacca ctggaaacg attaatttg gacgattac gaaaacccg ccggaatgt tcaggtttt ggtggcgat ttagatgaa caagacgaa gcaaacttt ttgtatttt tgtggcgaa tgtccggcc cgctttttg aaagaaaac gaatacaaa atccatact agattacac ggattgagg tcgtactac aagtgtgac cattgctcg tattcagca cgtcaaaaa ccccatttg ctcgcgcac aaaaacgtc cactcacaa cagtaccaa gatcggacg caagtgttg caaagtatg tacaaatcg aacaaccaa ccccctgat ttatacacc tgtttgaac aatgaagga gaaacgatt tggttggtg tctggttgt aatagcgaa gtcaaagat ttggaaatg gacgttgag agtctaagt gccatacta agtaaaccg ttgaagaat agtctaaac gttccgttg tcggggacc gagctcttc cagcagaag caagcgcag atgctcaag gagccttcg ccccctaaa gccagcgac ccccaattc gggagtcta atgcacggc aaccccaat ttcgtctac cccacgtac ctgaagaac ggcaaaatg aaagagaag cgttacaag tgccataaa tgccccagt gctttcgaa aagcgggag cagtacaag gtccacttg aacttgcac gggtcgaag cagcgctac aactgcgag ttttgcgac tattcggtc aagtattat gcgaattat gtgcaacac ttgaagaag cataaaatg aattcggag gctttggct gcgcgtgac gttgagatg gacgttgag gacgaagaa gttaccatt gaggctagt gatgagaag aagctttcg ctcggtgat cagcagacg ttgaatata ctcgagcaa cgattgcta gtcaatagt gataataag gaggaggag gagaagaag gttgtgacg tttagttgt ccgaattgt ccgtatgtg aaccatagg aaggacgcg gtggacaac caccagaag cgacacggg agtaaaagt aactacacg tgcgagcat tgtgattac tctgttccg caagtgcac tttttgagg gatcatgcc aagttgcac tttgtgccg aataataag ggacaagtg gatggctac atgtattgt gatagtttc aagctagtg gggaggaaa gtggacgaa gagaaggaa gatgtgatt tttgacgaa ggggcggag gaagttacc gacaagttt aataataac gacggggaa aaacagttt gttaatgta gacaccggc gaagtcgcc gaacggggg agtagtgat aaaatggat gagagttag
- >TC014806 RB
atgtccgtg ccgtcgatg gtacatcga tcaggagag cctctcacg acgactgcg cttttcgac gactttgac gacgaaaaa gacctcccc gagtgcaag gtgaagcga aactactcg tgcgccagc tgtgacttc ttcacgcaa aaccccagg cactacctc taccacctc cgcgatgtc cacgaggag aaagtgagg atctacgag tgccccaac tgtctctac gcctccaaa cacttccag aagctgcta aggcacacc aagatggtg cacggcgac aaggacgac gacgacagg gcggacacc ccgcccccg gacgacccc gaaacggtg ttcaagtgc tcggtgtgc gcgtttagc gcccgcacc cgcgcgcag ctcgaccgt cacgagcgc gccgaacac atcaagaca aagttcttc aggtgcacc aagtgcacg tacgccacc cacatgaag gcgcgtttc accaaacac gtgaaatac cactcgatg ccgatgatc aagtgcgac atgtgcgac ttccgcacc ccttacaag tggaacctg gaccgccac tgcaagaac cacaacggc aaaggtgcg ttccgatgc tcggcgtgc aacttcacc gccgacatc aagcaaagt ctcaccgtg cacgagatg aaccaccat gtgcctccc gtgggccag gcggccggt ttgggggtg ggacgccga cgcaacaag gtgggggcg agcgacgcc accgccgcc gaggaggcc gcacgccaa gaacaacag gtgccttct gaagtggaa aagaaagtg aagcacagc cctgagcaa gggtccttc gggccggat tttatcaac ccggatgat atcatccat catgccaat ggcaacgtt tatatcaaa aataagtgc aaactgtgc aatttcaag acggcttgg gcctcggaa atgacccgt cacgagcaa aaagtccac aactttaac cctgagccc aaaccgaaa aaaccgaca agacccata cccaacctc atccctata ccttcacaa acttcggtg ttaaaactt cccaagaag aataaggaa caagaaccg gaaatgagc gagcaagac attaacaat atttgtgcg aaatcggcc aatagtgcc ctcaaagac tttgcttcg ttgttcgac agtgaagat gttttcgaa aattgcgaa aacaaaatc cctgatctc atcccaacc actaaacaa accaccgat gaatttaaa caaaaaaac gcctctttc ttcgataaa ctcaaggag aaactgatg agcagcaat gggactaac tctatgacg tgtcaattg tgcggctat cagagcaaa tgcttgtct gaacaactc aaacatcag aaaaagtgc ggcaaggag agcaaccac cctccaagc ttaaccacg ttcaccagc tcctccagg tgccagtat tgccgtcaa aggtgcaag tcaagtcaa ggactttac acccaccaa ttgacctgt cccgagtat ctcaaagcg gccgaagaa tccgaaaac gagcttcga attgacgaa agtcagtcc gaagacggt gaaaacaac aacaaaccc caccctatg gaaaatcgc gtctttatt tggaacaac atcgagttt ccaatggat gtcgaagtc gacgattcc aagtatgag tacaaagtg gacgacaac gtctccctg gacctctcc gtgcgcacc caatcacca ggaggtagc gaacgcagc gagagcagc tacgtgggg gcccaagaa ttcgccaca acccatccc gttaccgac aagatcccc acccatggg aacgacatc accgtggcg caacacaaa cgcgtgttc aagtgccca cattgcacc ttttgggca tcaacagcg tcacgtttc cacgtgcat atcgtcggt catttgaat aaaaaaccc tttgaatgt tcactgtgt gcgtacaga tcgaattgg cgatgggac atcaccaaa catattaaa ttgaaatcg gtcagagac ccagcacat gaaagcgcc aaagtttta atgacggac gaaacaggc cgaagaaat tacaccaag tataataaa tacttgacg gagatacat gtgagtgga aatcaaagt ttagatacg agtggtggg tcgggaaca aggccgaaa catcacgat cgcaatact tcgccgact cagaataaa ggggattta ccgaaattg acgcgagcg ccgtctggg aatgatttt agtccgttg ttgaggcct ccgcctcca ttgaaagcc gccgatgga tcgttcttc aaggttgat aagaataaa aggaacaat atggataat aagaggacg tttttcaaa tgcaagaag tgcaatttt agggacgcc tctcgcgag atcctcttg gcgcacgtg aagggccac tacccgcag ggcctttcg acgcccagt ccaggtcag gacttgagt atgcgaggt tccagtcca gagaaagac gagcgtcac tgtcgcctg aaacacagc ggtgatata cgcgtcgtt agcaacaaa aacggtcaa gaaaaactc gaaattgaa gagcaaacc gacgaacct gaccatgtt gatgtaccc caagatggg gctttcaaa tgcaacgag tgccccttt tctagtgat agtcaagag gagctcgaa gaacacctt cctggccat acggcgacg aatgaaagt gtctccaag tgtcaattt tgcaattat ttcgtcaat caaaaagag agtcttttt gatcatttg cgactgcac ggaatcgca gatcctgaa gagttcctc agcaagttt tattcgagt cctgagggg gaccttggg aaaaggttc aagtgcatg gtgtgcccc tacgtgacc aactccaag tcgcagtac acctatcac aaacagttt cacaaaccg agagggggc caatacact tgcacccaa tgtagttac aatgtgagt aagcggcac ttgttgcac caacatttg aaagtgcat gggattagt attacgcct cagaagcaa aacggcgaa gtgatcgat cttgacgat atttccgac gaaatagag gaggtgtca tctggtggt ttcgaggga caaaacttc ccagatatc ccgctagtt tgggtctcg aaaaatgat aaattctcc aagatgttt aagtgtcgc tactgtcca catgtcaat ttacggaaa gttaacatc caagaacac gagaaaatg cacagtgtc agggagaaa aacccgaat tcgagtcgt ttgaacgat gtggaacac cgatgtacg gagtgtaac tacgtttgt aacaatgcc ggggtttta tcatcgcat tcaaaggtc caccaaggg ttgtacggg acggttcat tgtctcgta gatcaggtc aagtcagac gaggagcaa atccgcgaa ttgagcaag tttttaagt ccacgacca ctggaaacg attaatttg gacgattac gaaaacccg ccggaatgt tcaggtttt ggtggcgat ttagatgaa caagacgaa gcaaacttt ttgtatttt tgtggcgaa tgtccggcc cgctttttg aaagaaaac gaatacaaa atccatact agattacac ggattgagg tcgtactac aagtgtgac cattgctcg tattcagca cgtcaaaaa ccccatttg ctcgcgcac aaaaacgtc cactcacaa cagtaccaa gatcggacg caagtgttg caaagtatg tacaaatcg aacaaccaa ccccctgat ttatacacc tgtttgaac aatgaagga gaaacgatt tggttggtg tctggttgt aatagcgaa gtcaaagat ttggaaatg gacgttgag agtctaagt gccatacta agtaaaccg ttgaagaat agtctaaac gttccgttg tcggggacc gagctcttc cagcagaag caagcgcag atgctcaag gagccttcg ccccctaaa gccagcgac ccccaattc gggagtcta atgcacggc aaccccaat ttcgtctac cccacgtac ctgaagaac ggcaaaatg aaagagaag cgttacaag tgccataaa tgccccagt gctttcgaa aagcgggag cagtacaag gtccacttg aacttgcac gggtcgaag cagcgctac aactgcgag ttttgcgac tattcggtc aagtattat gcgaattat gtgcaacac ttgaagaag cataaaatg aattcggag gctttggct gcgcgtgac gttgagatg gacgttgag gacgaagaa gttaccatt gaggctagt gatgagaag aagctttcg ctcggtgat cagcagacg ttgaatata ctcgagcaa cgattgcta gtcaatagt gataataag gaggaggag gagaagaag gttgtgacg tttagttgt ccgaattgt ccgtatgtg aaccatagg aaggacgcg gtggacaac caccagaag cgacacggg agtaaaagt aactacacg tgcgagcat tgtgattac tctgttccg caagtgcac tttttgagg gatcatgcc aagttgcac tttgtgccg aataataag ggacaagtg gatggctac atgtattgt gatagtttc aagctagtg gggaggaaa gtggacgaa gagaaggaa gatgtgatt tttgacgaa ggggcggag gaagttacc gacaagttt aataataac gacggggaa aaacagttt gttaatgta gacaccggc gaagtcgcc gaacggggg agtagtgat aaaatggat gagagttag
- >TC014806 RA
ttcggtctg tgtatcatc aaggggtca gcaagtctc ggtttatct cttcgggca tcgcctctt tgtaatccc tctgatcga tgtccaact aactctgag gaggaatcc ataaagaac ccgcgccat ggcgtgcat catcgattg ccctccccg ttgctagcg acgctagtc gacaaagag aggcagagg gtgcggcac agcacagca cagcacagc acacgaggg tccgccggt ggccgcccc ggcagctga cgcagcagc agcagcgaa gaggaactc tcaccgtcg tgtaatacc gtcgtcgat ctgcagtgt tcctccggc cggggtctc gcgtgcatg tgcctcatc ctccgcgtg catgccaac tattgcgac atcttcgcc gaaggcttg tgcaactgc tggtggcga cgcccaccc aaccgacgc ttttagacc tcttgaagg tcgacaaaa ggtcgaacg acgccgccg tcgtccaag accgcaacg cacctccgc atacggtcg ctaatcgca ccacgtcct tggagcgct cttatcgat acgtaaaca gctccaagg tgaagacgt cgtcggatc cacacaggt gcacgggct agcatgtcc gtgccgtcg atggtacat cgatcagga gagcctctc acgacgact gcgcttttc gacgacttt gacgacgaa aaagacctc cccgagtgc aaggtgaag cgaaactac tcgtgcgcc agctgtgac ttcttcacg caaaacccc aggcactac ctctaccac ctccgcgat gtccacgag gagaaagtg aggatctac gagtgcccc aactgtctc tacgcctcc aaacacttc cagaagctg ctaaggcac accaagatg gtgcacggc gacaaggac gacgacgac agggcggac accccgccc ccggacgac cccgaaacg gtgttcaag tgctcggtg tgcgcgttt agcgcccgc acccgcgcg cagctcgac cgtcacgag cgcgccgaa cacatcaag acaaagttc ttcaggtgc accaagtgc acgtacgcc acccacatg aaggcgcgt ttcaccaaa cacgtgaaa taccactcg atgccgatg atcaagtgc gacatgtgc gacttccgc accccttac aagtggaac ctggaccgc cactgcaag aaccacaac ggcaaaggt gcgttccga tgctcggcg tgcaacttc accgccgac atcaagcaa agtctcacc gtgcacgag atgaaccac catgtgcct cccgtgggc caggcggcc ggtttgggg gtgggacgc cgacgcaac aaggtgggg gcgagcgac gccaccgcc gccgaggag gccgcacgc caagaacaa caggtgcct tctgaagtg gaaaagaaa gtgaagcac agccctgag caagggtcc ttcgggccg gattttatc aacccggat gatatcatc catcatgcc aatggcaac gtttatatc aaaaataag tgcaaactg tgcaatttc aagacggct tgggcctcg gaaatgacc cgtcacgag caaaaagtc cacaacttt aaccctgag cccaaaccg aaaaaaccg acaagaccc atacccaac ctcatccct ataccttca caaacttcg gtgttaaaa cttcccaag aagaataag gaacaagaa ccggaaatg agcgagcaa gacattaac aatatttgt gcgaaatcg gccaatagt gccctcaaa gactttgct tcgttgttc gacagtgaa gatgttttc gaaaattgc gaaaacaaa atccctgat ctcatccca accactaaa caaaccacc gatgaattt aaacaaaaa aacgcctct ttcttcgat aaactcaag gagaaactg atgagcagc aatgggact aactctatg acgtgtcaa ttgtgcggc tatcagagc aaatgcttg tctgaacaa ctcaaacat cagaaaaag tgcggcaag gagagcaac caccctcca agcttaacc acgttcacc agctcctcc aggtgccag tattgccgt caaaggtgc aagtcaagt caaggactt tacacccac caattgacc tgtcccgag tatctcaaa gcggccgaa gaatccgaa aacgagctt cgaattgac gaaagtcag tccgaagac ggtgaaaac aacaacaaa ccccaccct atggaaaat cgcgtcttt atttggaac aacatcgag tttccaatg gatgtcgaa gtcgacgat tccaagtat gagtacaaa gtggacgac aacgtctcc ctggacctc tccgtgcgc acccaatca ccaggaggt agcgaacgc agcgagagc agctacgtg ggggcccaa gaattcgcc acaacccat cccgttacc gacaagatc cccacccat gggaacgac atcaccgtg gcgcaacac aaacgcgtg ttcaagtgc ccacattgc accttttgg gcatcaaca gcgtcacgt ttccacgtg catatcgtc ggtcatttg aataaaaaa ccctttgaa tgttcactg tgtgcgtac agatcgaat tggcgatgg gacatcacc aaacatatt aaattgaaa tcggtcaga gacccagca catgaaagc gccaaagtt ttaatgacg gacgaaaca ggccgaaga aattacacc aagtataat aaatacttg acggagata catgtgagt ggaaatcaa agtttagat acgagtggt gggtcggga acaaggccg aaacatcac gatcgcaat acttcgccg actcagaat aaaggggat ttaccgaaa ttgacgcga gcgccgtct gggaatgat tttagtccg ttgttgagg cctccgcct ccattgaaa gccgccgat ggatcgttc ttcaaggtt gataagaat aaaaggaac aatatggat aataagagg acgtttttc aaatgcaag aagtgcaat tttagggac gcctctcgc gagatcctc ttggcgcac gtgaagggc cactacccg cagggcctt tcgacgccc agtccaggt caggacttg agtatgcga ggttccagt ccagagaaa gacgaggca agtgttagc acgtcagcg aaagggagc caagcgccc ttccgctgc ggtcactgc aaccaggta tcaaactgg aagcatgtc attcagcgt cactgtcgc ctgaaacac agcggtgat atacgcgtc gttagcaac aaaaacggt caagaaaaa ctcgaaatt gaagagcaa accgacgaa cctgaccat gttgatgta ccccaagat ggggctttc aaatgcaac gagtgcccc ttttctagt gatagtcaa gaggagctc gaagaacac cttcctggc catacggcg acgaatgaa agtgtctcc aagtgtcaa ttttgcaat tatttcgtc aatcaaaaa gagagtctt tttgatcat ttgcgactg cacggaatc gcagatcct gaagagttc ctcagcaag ttttattcg agtcctgag ggggacctt gggaaaagg ttcaagtgc atggtgtgc ccctacgtg accaactcc aagtcgcag tacacctat cacaaacag tttcacaaa ccgagaggg ggccaatac acttgcacc caatgtagt tacaatgtg agtaagcgg cacttgttg caccaacat ttgaaagtg catgggatt agtattacg cctcagaag caaaacggc gaagtgatc gatcttgac gatatttcc gacgaaata gaggaggtg tcatctggt ggtttcgag ggacaaaac ttcccagat atcccgcta gtttgggtc tcgaaaaat gataaattc tccaagatg tttaagtgt cgctactgt ccacatgtc aatttacgg aaagttaac atccaagaa cacgagaaa atgcacagt gtcagggag aaaaacccg aattcgagt cgtttgaac gatgtggaa caccgatgt acggagtgt aactacgtt tgtaacaat gccggggtt ttatcatcg cattcaaag gtccaccaa gggttgtac gggacggtt cattgtctc gtagatcag gtcaagtca gacgaggag caaatccgc gaattgagc aagttttta agtccacga ccactggaa acgattaat ttggacgat tacgaaaac ccgccggaa tgttcaggt tttggtggc gatttagat gaacaagac gaagcaaac tttttgtat ttttgtggc gaatgtccg gcccgcttt ttgaaagaa aacgaatac aaaatccat actagatta cacggattg aggtcgtac tacaagtgt gaccattgc tcgtattca gcacgtcaa aaaccccat ttgctcgcg cacaaaaac gtccactca caacagtac caagatcgg acgcaagtg ttgcaaagt atgtacaaa tcgaacaac caaccccct gatttatac acctgtttg aacaatgaa ggagaaacg atttggttg gtgtctggt tgtaatagc gaagtcaaa gatttggaa atggacgtt gagagtcta agtgccata ctaagtaaa ccgttgaag aatagtcta aacgttccg ttgtcgggg accgagctc ttccagcag aagcaagcg cagatgctc aaggagcct tcgccccct aaagccagc gacccccaa ttcgggagt ctaatgcac ggcaacccc aatttcgtc taccccacg tacctgaag aacggcaaa atgaaagag aagcgttac aagtgccat aaatgcccc agtgctttc gaaaagcgg gagcagtac aaggtccac ttgaacttg cacgggtcg aagcagcgc tacaactgc gagttttgc gactattcg gtcaagtat tatgcgaat tatgtgcaa cacttgaag aagcataaa atgaattcg gaggctttg gctgcgcgt gacgttgag atggacgtt gaggacgaa gaagttacc attgaggct agtgatgag aagaagctt tcgctcggt gatcagcag acgttgaat atactcgag caacgattg ctagtcaat agtgataat aaggaggag gaggagaag aaggttgtg acgtttagt tgtccgaat tgtccgtat gtgaaccat aggaaggac gcggtggac aaccaccag aagcgacac gggagtaaa agtaactac acgtgcgag cattgtgat tactctgtt ccgcaagtg cactttttg agggatcat gccaagttg cactttgtg ccgaataat aagggacaa gtggatggc tacatgtat tgtgatagt ttcaagcta gtggggagg aaagtggac gaagagaag gaagatgtg atttttgac gaaggggcg gaggaagtt accgacaag tttaataat aacgacggg gaaaaacag tttgttaat gtagacacc ggcgaagtc gccgaacgg gggagtagt gataaaatg gatgagagt tagtcagat ttctagtga taattcttc agaggacta acaggtttg ccttagtcg taatataga gtatttcta cagagtatt ttcacatat tgatagcaa attttcatc ttgtagtat taattttaa agaattttc ttacttgaa tagtgttgt atcttgtat ttattttat ctagttgat gttcgttcg tcatttcat gggtttatt aattaataa cgccaaagc atttaatgc caaattttg ccattattg ttgtattat attgtttac agagaatat atgagttat tgcatt
- >TC014806 RB
ttcggtctg tgtatcatc aaggggtca gcaagtctc ggtttatct cttcgggca tcgcctctt tgtaatccc tctgatcga tgtccaact aactctgag gaggaatcc ataaagaac ccgcgccat ggcgtgcat catcgattg ccctccccg ttgctagcg acgctagtc gacaaagag aggcagagg gtgcggcac agcacagca cagcacagc acacgaggg tccgccggt ggccgcccc ggcagctga cgcagcagc agcagcgaa gaggaactc tcaccgtcg tgtaatacc gtcgtcgat ctgcagtgt tcctccggc cggggtctc gcgtgcatg tgcctcatc ctccgcgtg catgccaac tattgcgac atcttcgcc gaaggcttg tgcaactgc tggtggcga cgcccaccc aaccgacgc ttttagacc tcttgaagg tcgacaaaa ggtcgaacg acgccgccg tcgtccaag accgcaacg cacctccgc atacggtcg ctaatcgca ccacgtcct tggagcgct cttatcgat acgtaaaca gctccaagg tgaagacgt cgtcggatc cacacaggt gcacgggct agcatgtcc gtgccgtcg atggtacat cgatcagga gagcctctc acgacgact gcgcttttc gacgacttt gacgacgaa aaagacctc cccgagtgc aaggtgaag cgaaactac tcgtgcgcc agctgtgac ttcttcacg caaaacccc aggcactac ctctaccac ctccgcgat gtccacgag gagaaagtg aggatctac gagtgcccc aactgtctc tacgcctcc aaacacttc cagaagctg ctaaggcac accaagatg gtgcacggc gacaaggac gacgacgac agggcggac accccgccc ccggacgac cccgaaacg gtgttcaag tgctcggtg tgcgcgttt agcgcccgc acccgcgcg cagctcgac cgtcacgag cgcgccgaa cacatcaag acaaagttc ttcaggtgc accaagtgc acgtacgcc acccacatg aaggcgcgt ttcaccaaa cacgtgaaa taccactcg atgccgatg atcaagtgc gacatgtgc gacttccgc accccttac aagtggaac ctggaccgc cactgcaag aaccacaac ggcaaaggt gcgttccga tgctcggcg tgcaacttc accgccgac atcaagcaa agtctcacc gtgcacgag atgaaccac catgtgcct cccgtgggc caggcggcc ggtttgggg gtgggacgc cgacgcaac aaggtgggg gcgagcgac gccaccgcc gccgaggag gccgcacgc caagaacaa caggtgcct tctgaagtg gaaaagaaa gtgaagcac agccctgag caagggtcc ttcgggccg gattttatc aacccggat gatatcatc catcatgcc aatggcaac gtttatatc aaaaataag tgcaaactg tgcaatttc aagacggct tgggcctcg gaaatgacc cgtcacgag caaaaagtc cacaacttt aaccctgag cccaaaccg aaaaaaccg acaagaccc atacccaac ctcatccct ataccttca caaacttcg gtgttaaaa cttcccaag aagaataag gaacaagaa ccggaaatg agcgagcaa gacattaac aatatttgt gcgaaatcg gccaatagt gccctcaaa gactttgct tcgttgttc gacagtgaa gatgttttc gaaaattgc gaaaacaaa atccctgat ctcatccca accactaaa caaaccacc gatgaattt aaacaaaaa aacgcctct ttcttcgat aaactcaag gagaaactg atgagcagc aatgggact aactctatg acgtgtcaa ttgtgcggc tatcagagc aaatgcttg tctgaacaa ctcaaacat cagaaaaag tgcggcaag gagagcaac caccctcca agcttaacc acgttcacc agctcctcc aggtgccag tattgccgt caaaggtgc aagtcaagt caaggactt tacacccac caattgacc tgtcccgag tatctcaaa gcggccgaa gaatccgaa aacgagctt cgaattgac gaaagtcag tccgaagac ggtgaaaac aacaacaaa ccccaccct atggaaaat cgcgtcttt atttggaac aacatcgag tttccaatg gatgtcgaa gtcgacgat tccaagtat gagtacaaa gtggacgac aacgtctcc ctggacctc tccgtgcgc acccaatca ccaggaggt agcgaacgc agcgagagc agctacgtg ggggcccaa gaattcgcc acaacccat cccgttacc gacaagatc cccacccat gggaacgac atcaccgtg gcgcaacac aaacgcgtg ttcaagtgc ccacattgc accttttgg gcatcaaca gcgtcacgt ttccacgtg catatcgtc ggtcatttg aataaaaaa ccctttgaa tgttcactg tgtgcgtac agatcgaat tggcgatgg gacatcacc aaacatatt aaattgaaa tcggtcaga gacccagca catgaaagc gccaaagtt ttaatgacg gacgaaaca ggccgaaga aattacacc aagtataat aaatacttg acggagata catgtgagt ggaaatcaa agtttagat acgagtggt gggtcggga acaaggccg aaacatcac gatcgcaat acttcgccg actcagaat aaaggggat ttaccgaaa ttgacgcga gcgccgtct gggaatgat tttagtccg ttgttgagg cctccgcct ccattgaaa gccgccgat ggatcgttc ttcaaggtt gataagaat aaaaggaac aatatggat aataagagg acgtttttc aaatgcaag aagtgcaat tttagggac gcctctcgc gagatcctc ttggcgcac gtgaagggc cactacccg cagggcctt tcgacgccc agtccaggt caggacttg agtatgcga ggttccagt ccagagaaa gacgagcgt cactgtcgc ctgaaacac agcggtgat atacgcgtc gttagcaac aaaaacggt caagaaaaa ctcgaaatt gaagagcaa accgacgaa cctgaccat gttgatgta ccccaagat ggggctttc aaatgcaac gagtgcccc ttttctagt gatagtcaa gaggagctc gaagaacac cttcctggc catacggcg acgaatgaa agtgtctcc aagtgtcaa ttttgcaat tatttcgtc aatcaaaaa gagagtctt tttgatcat ttgcgactg cacggaatc gcagatcct gaagagttc ctcagcaag ttttattcg agtcctgag ggggacctt gggaaaagg ttcaagtgc atggtgtgc ccctacgtg accaactcc aagtcgcag tacacctat cacaaacag tttcacaaa ccgagaggg ggccaatac acttgcacc caatgtagt tacaatgtg agtaagcgg cacttgttg caccaacat ttgaaagtg catgggatt agtattacg cctcagaag caaaacggc gaagtgatc gatcttgac gatatttcc gacgaaata gaggaggtg tcatctggt ggtttcgag ggacaaaac ttcccagat atcccgcta gtttgggtc tcgaaaaat gataaattc tccaagatg tttaagtgt cgctactgt ccacatgtc aatttacgg aaagttaac atccaagaa cacgagaaa atgcacagt gtcagggag aaaaacccg aattcgagt cgtttgaac gatgtggaa caccgatgt acggagtgt aactacgtt tgtaacaat gccggggtt ttatcatcg cattcaaag gtccaccaa gggttgtac gggacggtt cattgtctc gtagatcag gtcaagtca gacgaggag caaatccgc gaattgagc aagttttta agtccacga ccactggaa acgattaat ttggacgat tacgaaaac ccgccggaa tgttcaggt tttggtggc gatttagat gaacaagac gaagcaaac tttttgtat ttttgtggc gaatgtccg gcccgcttt ttgaaagaa aacgaatac aaaatccat actagatta cacggattg aggtcgtac tacaagtgt gaccattgc tcgtattca gcacgtcaa aaaccccat ttgctcgcg cacaaaaac gtccactca caacagtac caagatcgg acgcaagtg ttgcaaagt atgtacaaa tcgaacaac caaccccct gatttatac acctgtttg aacaatgaa ggagaaacg atttggttg gtgtctggt tgtaatagc gaagtcaaa gatttggaa atggacgtt gagagtcta agtgccata ctaagtaaa ccgttgaag aatagtcta aacgttccg ttgtcgggg accgagctc ttccagcag aagcaagcg cagatgctc aaggagcct tcgccccct aaagccagc gacccccaa ttcgggagt ctaatgcac ggcaacccc aatttcgtc taccccacg tacctgaag aacggcaaa atgaaagag aagcgttac aagtgccat aaatgcccc agtgctttc gaaaagcgg gagcagtac aaggtccac ttgaacttg cacgggtcg aagcagcgc tacaactgc gagttttgc gactattcg gtcaagtat tatgcgaat tatgtgcaa cacttgaag aagcataaa atgaattcg gaggctttg gctgcgcgt gacgttgag atggacgtt gaggacgaa gaagttacc attgaggct agtgatgag aagaagctt tcgctcggt gatcagcag acgttgaat atactcgag caacgattg ctagtcaat agtgataat aaggaggag gaggagaag aaggttgtg acgtttagt tgtccgaat tgtccgtat gtgaaccat aggaaggac gcggtggac aaccaccag aagcgacac gggagtaaa agtaactac acgtgcgag cattgtgat tactctgtt ccgcaagtg cactttttg agggatcat gccaagttg cactttgtg ccgaataat aagggacaa gtggatggc tacatgtat tgtgatagt ttcaagcta gtggggagg aaagtggac gaagagaag gaagatgtg atttttgac gaaggggcg gaggaagtt accgacaag tttaataat aacgacggg gaaaaacag tttgttaat gtagacacc ggcgaagtc gccgaacgg gggagtagt gataaaatg gatgagagt tagtcagat ttctagtga taattcttc agaggacta acaggtttg ccttagtcg taatataga gtatttcta cagagtatt ttcacatat tgatagcaa attttcatc ttgtagtat taattttaa agaattttc ttacttgaa tagtgttgt atcttgtat ttattttat ctagttgat gttcgttcg tcatttcat gggtttatt aattaataa cgccaaagc atttaatgc caaattttg ccattattg ttgtattat attgtttac agagaatat atgagttat tgcatt
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