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Gene information
General information
TC014795 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15144878..15148942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014795
    iBeetle screen iB_05696

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014795 RA
      MLTIVWLCL LVTLASGAP EADTTQGRL RGKYQTTKG 
      NRTFSAFEG IPFAKPPLG ALRFKAPVP GDKWDGVKE 
      ATSRHNVCP QRDIYRRAT LIEGDEDCL YLNVYTPQV 
      GQSATPLPV MVFFHGGGW LCGGGNSMW YGPEILLDR 
      DVVLVVPNY RLGALGFLS TGDSVCPGN NGMKDQNLA 
      LRWVKDNIG EFGGDASKI TIFGESAGG ASVQLHMVS 
      PLSKGLFHT AISQSGTAH CLWAVAPEN QGAKHAKML 
      AEEFGCDTG SSEALVECL RHIPAYDLV SKDQIFMKW 
      DTDPMIPFK PTIEPATEG AFLVEHPSE TMKKGTFSQ 
      VPWIVGLNT NDGALRAAG IFGNPYLID ELDDKFNEV 
      VPISLLYDE TSSRAKEVT NKIRKFYFG ASKITESSR 
      FDVVDMYTD GWFLNGADE AIRYYLKYG KKPVYYYLF 
      GHRGVASFT EIFGDKEKD YGVCHADEL QYLFPIGDG 
      LFPDKPPSP DDKKMAKIM TTLWTNFAK FGDPTPVTN 
      EDIPNKWTP VISKDMEYY NINLDSLEM RQGLFKSRA 
      EFWRSLPIH AKTQEIKDE L
    CDS sequence
    • >TC014795 RA
      atgttgact atagtctgg ttgtgcctc ttggtgacg 
      ctggcgtcg ggggcgccc gaggccgac accacccag 
      ggccggctc cgggggaag taccagacg acgaaaggc 
      aaccgcacc ttcagcgcc ttcgagggc attcccttc 
      gccaagccg cccttgggg gccctcaga tttaaggcg 
      ccggtccct ggggataag tgggatggg gtcaaggag 
      gccacctcc aggcacaac gtgtgccca cagagggat 
      atctacagg agggccacg ctgattgag ggggacgag 
      gactgcctc tacctcaat gtttatacg ccacaggtg 
      ggtcaatct gccacccct ctacctgtc atggtgttc 
      ttccacggg ggcggttgg ctttgtggc ggcggcaac 
      tccatgtgg tacggcccc gaaattctc cttgaccgt 
      gacgttgtc cttgttgtc cccaattac agattagga 
      gcgctggga ttcctaagc actggggac tccgtctgt 
      cccggaaac aacggcatg aaggaccag aacctggcc 
      ctgaggtgg gtcaaagac aacatcggg gagttcggg 
      ggcgatgcc tcaaaaatc accattttt ggcgaatcg 
      gcaggcggt gccagcgtc cagctgcac atggtgtcc 
      cctctcagc aaagggctt ttccacacg gcaatctca 
      cagagcggc acggcgcac tgtctctgg gccgtggct 
      cccgaaaat caaggggct aaacatgcc aaaatgttg 
      gctgaggaa tttgggtgc gataccggt tcaagtgag 
      gctttagtc gagtgtttg aggcacatt cctgcctat 
      gatctagtc tccaaagac caaattttt atgaaatgg 
      gacactgat cccatgatt cccttcaaa ccgaccatt 
      gaacccgcc actgaaggt gccttccta gtcgaacac 
      cccagcgag accatgaaa aaaggtact ttcagtcaa 
      gtaccatgg atcgtgggg ttgaatacc aacgatggg 
      gcgcttcgc gccgctggt atttttggt aacccgtat 
      ttaattgac gaacttgac gacaagttc aatgaagtt 
      gtgcccatt tcgctactt tacgacgag acaagtagt 
      agggctaag gaagtgacc aacaaaatt cgcaaattt 
      tactttggg gcgtcgaaa attaccgaa agttcgcgc 
      tttgatgtt gtggatatg tacactgat ggctggttc 
      ctgaacggt gccgacgag gccattcgg tactacctc 
      aaatatggc aaaaagccg gtctattat tatttattt 
      ggccatcgt ggcgttgcc agttttacc gaaattttc 
      ggagacaag gaaaaggac tatggagtg tgtcacgcg 
      gacgaactt cagtaccta ttccccata ggtgatggg 
      cttttccca gacaagccc ccaagtccg gacgacaaa 
      aaaatggcc aaaatcatg accacactt tggaccaat 
      tttgcaaaa ttcggcgat ccaactcca gttaccaac 
      gaagacatc ccaaataag tggactcct gtcatatct 
      aaagacatg gaatactac aacatcaac ctggacagc 
      ctggaaatg agacaagga ctgttcaaa tcacgcgcc 
      gaattttgg cgttcttta ccaattcat gcaaaaaca 
      caggaaata aaagatgaa ctgtga
    mRNA sequence
    • >TC014795 RA
      tctcatcgg aagacaaat gttgactat agtctggtt 
      gtgcctctt ggtgacgct ggcgtcggg ggcgcccga 
      ggccgacac cacccaggg ccggctccg ggggaagta 
      ccagacgac gaaaggcaa ccgcacctt cagcgcctt 
      cgagggcat tcccttcgc caagccgcc cttgggggc 
      cctcagatt taaggcgcc ggtccctgg ggataagtg 
      ggatggggt caaggaggc cacctccag gcacaacgt 
      gtgcccaca gagggatat ctacaggag ggccacgct 
      gattgaggg ggacgagga ctgcctcta cctcaatgt 
      ttatacgcc acaggtggg tcaatctgc cacccctct 
      acctgtcat ggtgttctt ccacggggg cggttggct 
      ttgtggcgg cggcaactc catgtggta cggccccga 
      aattctcct tgaccgtga cgttgtcct tgttgtccc 
      caattacag attaggagc gctgggatt cctaagcac 
      tggggactc cgtctgtcc cggaaacaa cggcatgaa 
      ggaccagaa cctggccct gaggtgggt caaagacaa 
      catcgggga gttcggggg cgatgcctc aaaaatcac 
      catttttgg cgaatcggc aggcggtgc cagcgtcca 
      gctgcacat ggtgtcccc tctcagcaa agggctttt 
      ccacacggc aatctcaca gagcggcac ggcgcactg 
      tctctgggc cgtggctcc cgaaaatca aggggctaa 
      acatgccaa aatgttggc tgaggaatt tgggtgcga 
      taccggttc aagtgaggc tttagtcga gtgtttgag 
      gcacattcc tgcctatga tctagtctc caaagacca 
      aatttttat gaaatggga cactgatcc catgattcc 
      cttcaaacc gaccattga acccgccac tgaaggtgc 
      cttcctagt cgaacaccc cagcgagac catgaaaaa 
      aggtacttt cagtcaagt accatggat cgtggggtt 
      gaataccaa cgatggggc gcttcgcgc cgctggtat 
      ttttggtaa cccgtattt aattgacga acttgacga 
      caagttcaa tgaagttgt gcccatttc gctacttta 
      cgacgagac aagtagtag ggctaagga agtgaccaa 
      caaaattcg caaatttta ctttggggc gtcgaaaat 
      taccgaaag ttcgcgctt tgatgttgt ggatatgta 
      cactgatgg ctggttcct gaacggtgc cgacgaggc 
      cattcggta ctacctcaa atatggcaa aaagccggt 
      ctattatta tttatttgg ccatcgtgg cgttgccag 
      ttttaccga aattttcgg agacaagga aaaggacta 
      tggagtgtg tcacgcgga cgaacttca gtacctatt 
      ccccatagg tgatgggct tttcccaga caagccccc 
      aagtccgga cgacaaaaa aatggccaa aatcatgac 
      cacactttg gaccaattt tgcaaaatt cggcgatcc 
      aactccagt taccaacga agacatccc aaataagtg 
      gactcctgt catatctaa agacatgga atactacaa 
      catcaacct ggacagcct ggaaatgag acaaggact 
      gttcaaatc acgcgccga attttggcg ttctttacc 
      aattcatgc aaaaacaca ggaaataaa agatgaact 
      gtgacaaaa tctttcatt tcgtccctt gcttcctct 
      tcttctttt ccttggctt tgcgtttct cctcccgcg 
      tgagcttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY