Status
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Gene information
General information
TC014793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15136278..15138950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014793
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014793 RA
      MWPVLLDMT KEESMQSLR NLELEAYAH LVSALRAQG 
PLNPDKRKL LKDTSQVLN IPQDRHKAE IRRAVNDEK 
LNTIAYHVT GLTESLEDW AQEGRRSVP LLPRMAPQT 
AYSAIADEA AENANQTNK QLPYPAATE RKRPPMQPP 
VPTAPETSK GAQFRVPET PKIEDSKKR KLPICTENS 
SLAQHLLGP PKMSRTQQI YRQRTKGAI LKEQMKLKP 
QSKIPFNPI MQSSNGPKV VLPPSGEET IEQIQNEIN 
NYPQKSDAP SCSNENQAP RAKLFTPTK PTQSLTFKQ 
ITTAGTSDV KLCPKKTIT KPGTPLGQK LIVVSNAQT 
IHTSSILQK TLSIPLVKN ISVKNFEKF KIVTTSSPQ 
LVTAANNIN SLKHKVVTV KTNPTTKKV IPLSLLNSK 
GGIKVLPIG GKIVGKTTT STSSPPLYI VNTVAPKTT 
TPAVVTEAP PSVSKENGK SSVLGDILK ASGVTTDDF 
DTQIEEFRI PNNHVAEEQ VEQVEEEEQ VTSSEKGDF 
FILPEGEVV EMKSDSTKT NEEQFSSAD VVTDDSETV 
ILQVQSSEL TSVGDAAN
    CDS sequence
    • >TC014793 RA
      atgtggccc gtgctgttg gatatgaca aaagaagaa 
tccatgcaa agtttgcgg aatttagaa ctggaagcc 
tatgcccat ctcgtgtcg gcgctgcga gcccaaggc 
ccccttaac cccgacaaa cgcaaactc ctgaaggac 
accagccag gtgcttaat atcccacag gaccgccac 
aaagccgaa attcggaga gccgtcaac gacgaaaaa 
ctcaacacg atcgcatat catgttaca ggcttgact 
gaatcgctg gaagattgg gcgcaagag gggcgcagg 
tccgtgccg ctgctaccc agaatggcc ccccagacc 
gcctactcc gccatcgcc gatgaggcg gccgaaaac 
gccaaccag accaacaaa caactgccc tatcctgcc 
gccaccgag cgaaaacgg cccccaatg cagcccccc 
gtgccaacg gcgccagaa acgagcaag ggggcgcag 
ttccgagtc ccagaaaca cccaaaatt gaagacagc 
aagaagcgg aaactaccc atttgtacg gaaaatagc 
agtctcgcg cagcacctc ctaggcccc ccgaagatg 
agtcggact caacaaata tacaggcaa cggacgaaa 
ggggcaata ctgaaagaa cagatgaag ctgaagccg 
cagtcaaaa atcccattt aatccgatt atgcagtcg 
tcaaacggc cccaaagta gtgttgccc ccaagtggc 
gaagaaaca atcgagcag attcaaaac gagataaat 
aattacccc caaaaatct gacgcccca agttgttcc 
aatgagaat caagcacct cgtgctaaa ctcttcact 
ccaactaaa cccacccag agtttaact ttcaaacaa 
atcacaaca gcaggcact agtgacgtg aaattgtgc 
ccaaaaaag accatcact aaaccgggg actcccctg 
ggacagaaa ctaatcgtt gtgtcgaac gctcaaacg 
attcatacc tctagtatt ctacaaaag actttgtcg 
ataccgttg gttaaaaat atctctgtg aaaaatttc 
gaaaagttt aagattgtc acgacaagt agcccacag 
ctcgtcact gccgccaat aatatcaat tcgttgaaa 
cataaagtt gtgactgtt aaaacaaat ccgacgact 
aagaaagtg attccgctc tcgttgctt aattcaaaa 
ggggggatt aaagtgttg ccaattggg ggtaaaata 
gtggggaag acaacaacg agcacctcc tcgccccca 
ttgtacatc gtgaataca gtggccccc aaaaccacg 
acgcccgca gtggtgaca gaggcgccg cccagtgtg 
agtaaagaa aatgggaaa tcgtcggtt ttgggcgat 
attttgaag gcgtctggt gttacaact gacgatttt 
gatacgcaa attgaggag tttagaatt ccgaataat 
catgtggct gaggaacaa gtggagcaa gttgaagag 
gaggaacaa gttacgtcg agtgaaaag ggcgatttt 
tttattctg cctgaaggt gaagttgtg gaaatgaaa 
agtgacagt acaaaaaca aacgaagaa caattttca 
agcgctgat gtcgtgaca gatgatagt gaaacagtg 
attttgcaa gttcagagt tcggaatta acaagtgtt 
ggtgatgct gctaactga
    mRNA sequence
    • >TC014793 RA
      ccaatttaa aaacgtaaa gttggcgcc gttcctcga 
tcaggttgt caaactttc gcaaagaat ttttccaaa 
ataaaaaca ataaatttg gaaaatagg gaaaactca 
gcgacggca tttagcaat tttggaaat cacttcaaa 
tgatgtgaa aattctggt acaggcgag acaaccctt 
gcgcaaaaa gagtcgcta gtgaacata accacaaaa 
aataagacg ctgtcatta ctgtaaaaa ttgattttt 
ttcgtctat tttccactg ggtattgtt aaatatgcc 
tttgatttg agcaagcaa caatgtggc ccgtgctgt 
tggatatga caaaagaag aatccatgc aaagtttgc 
ggaatttag aactggaag cctatgccc atctcgtgt 
cggcgctgc gagcccaag gccccctta accccgaca 
aacgcaaac tcctgaagg acaccagcc aggtgctta 
atatcccac aggaccgcc acaaagccg aaattcgga 
gagccgtca acgacgaaa aactcaaca cgatcgcat 
atcatgtta caggcttga ctgaatcgc tggaagatt 
gggcgcaag aggggcgca ggtccgtgc cgctgctac 
ccagaatgg ccccccaga ccgcctact ccgccatcg 
ccgatgagg cggccgaaa acgccaacc agaccaaca 
aacaactgc cctatcctg ccgccaccg agcgaaaac 
ggcccccaa tgcagcccc ccgtgccaa cggcgccag 
aaacgagca agggggcgc agttccgag tcccagaaa 
cacccaaaa ttgaagaca gcaagaagc ggaaactac 
ccatttgta cggaaaata gcagtctcg cgcagcacc 
tcctaggcc ccccgaaga tgagtcgga ctcaacaaa 
tatacaggc aacggacga aaggggcaa tactgaaag 
aacagatga agctgaagc cgcagtcaa aaatcccat 
ttaatccga ttatgcagt cgtcaaacg gccccaaag 
tagtgttgc ccccaagtg gcgaagaaa caatcgagc 
agattcaaa acgagataa ataattacc cccaaaaat 
ctgacgccc caagttgtt ccaatgaga atcaagcac 
ctcgtgcta aactcttca ctccaacta aacccaccc 
agagtttaa ctttcaaac aaatcacaa cagcaggca 
ctagtgacg tgaaattgt gcccaaaaa agaccatca 
ctaaaccgg ggactcccc tgggacaga aactaatcg 
ttgtgtcga acgctcaaa cgattcata cctctagta 
ttctacaaa agactttgt cgataccgt tggttaaaa 
atatctctg tgaaaaatt tcgaaaagt ttaagattg 
tcacgacaa gtagcccac agctcgtca ctgccgcca 
ataatatca attcgttga aacataaag ttgtgactg 
ttaaaacaa atccgacga ctaagaaag tgattccgc 
tctcgttgc ttaattcaa aagggggga ttaaagtgt 
tgccaattg ggggtaaaa tagtgggga agacaacaa 
cgagcacct cctcgcccc cattgtaca tcgtgaata 
cagtggccc ccaaaacca cgacgcccg cagtggtga 
cagaggcgc cgcccagtg tgagtaaag aaaatggga 
aatcgtcgg ttttgggcg atattttga aggcgtctg 
gtgttacaa ctgacgatt ttgatacgc aaattgagg 
agtttagaa ttccgaata atcatgtgg ctgaggaac 
aagtggagc aagttgaag aggaggaac aagttacgt 
cgagtgaaa agggcgatt tttttattc tgcctgaag 
gtgaagttg tggaaatga aaagtgaca gtacaaaaa 
caaacgaag aacaatttt caagcgctg atgtcgtga 
cagatgata gtgaaacag tgattttgc aagttcaga 
gttcggaat taacaagtg ttggtgatg ctgctaact 
gatatttat ttattttct aatttatta ttattaaat 
taatccgtt gactttttt taagatgca ttagttcaa 
ctttttgta cattatcgc tatatttta tcagacgtt 
ttgctcaat aaatgttca atcactttg tgttgccaa 
tcaaaattc atgtagcag ttttgcgta ttttagcat 
tttactgaa aattttgta catatgtaa tagaaaatg 
attgtagtt aaacaaatt cattgaata tattctgta 
cgtttaaat aaaccattt ttatttta
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