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Gene information
General information
TC014793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15136278..15138950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014793
    iBeetle screen iB_05695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014793 RA
      MWPVLLDMT KEESMQSLR NLELEAYAH LVSALRAQG 
      PLNPDKRKL LKDTSQVLN IPQDRHKAE IRRAVNDEK 
      LNTIAYHVT GLTESLEDW AQEGRRSVP LLPRMAPQT 
      AYSAIADEA AENANQTNK QLPYPAATE RKRPPMQPP 
      VPTAPETSK GAQFRVPET PKIEDSKKR KLPICTENS 
      SLAQHLLGP PKMSRTQQI YRQRTKGAI LKEQMKLKP 
      QSKIPFNPI MQSSNGPKV VLPPSGEET IEQIQNEIN 
      NYPQKSDAP SCSNENQAP RAKLFTPTK PTQSLTFKQ 
      ITTAGTSDV KLCPKKTIT KPGTPLGQK LIVVSNAQT 
      IHTSSILQK TLSIPLVKN ISVKNFEKF KIVTTSSPQ 
      LVTAANNIN SLKHKVVTV KTNPTTKKV IPLSLLNSK 
      GGIKVLPIG GKIVGKTTT STSSPPLYI VNTVAPKTT 
      TPAVVTEAP PSVSKENGK SSVLGDILK ASGVTTDDF 
      DTQIEEFRI PNNHVAEEQ VEQVEEEEQ VTSSEKGDF 
      FILPEGEVV EMKSDSTKT NEEQFSSAD VVTDDSETV 
      ILQVQSSEL TSVGDAAN
    CDS sequence
    • >TC014793 RA
      atgtggccc gtgctgttg gatatgaca aaagaagaa 
      tccatgcaa agtttgcgg aatttagaa ctggaagcc 
      tatgcccat ctcgtgtcg gcgctgcga gcccaaggc 
      ccccttaac cccgacaaa cgcaaactc ctgaaggac 
      accagccag gtgcttaat atcccacag gaccgccac 
      aaagccgaa attcggaga gccgtcaac gacgaaaaa 
      ctcaacacg atcgcatat catgttaca ggcttgact 
      gaatcgctg gaagattgg gcgcaagag gggcgcagg 
      tccgtgccg ctgctaccc agaatggcc ccccagacc 
      gcctactcc gccatcgcc gatgaggcg gccgaaaac 
      gccaaccag accaacaaa caactgccc tatcctgcc 
      gccaccgag cgaaaacgg cccccaatg cagcccccc 
      gtgccaacg gcgccagaa acgagcaag ggggcgcag 
      ttccgagtc ccagaaaca cccaaaatt gaagacagc 
      aagaagcgg aaactaccc atttgtacg gaaaatagc 
      agtctcgcg cagcacctc ctaggcccc ccgaagatg 
      agtcggact caacaaata tacaggcaa cggacgaaa 
      ggggcaata ctgaaagaa cagatgaag ctgaagccg 
      cagtcaaaa atcccattt aatccgatt atgcagtcg 
      tcaaacggc cccaaagta gtgttgccc ccaagtggc 
      gaagaaaca atcgagcag attcaaaac gagataaat 
      aattacccc caaaaatct gacgcccca agttgttcc 
      aatgagaat caagcacct cgtgctaaa ctcttcact 
      ccaactaaa cccacccag agtttaact ttcaaacaa 
      atcacaaca gcaggcact agtgacgtg aaattgtgc 
      ccaaaaaag accatcact aaaccgggg actcccctg 
      ggacagaaa ctaatcgtt gtgtcgaac gctcaaacg 
      attcatacc tctagtatt ctacaaaag actttgtcg 
      ataccgttg gttaaaaat atctctgtg aaaaatttc 
      gaaaagttt aagattgtc acgacaagt agcccacag 
      ctcgtcact gccgccaat aatatcaat tcgttgaaa 
      cataaagtt gtgactgtt aaaacaaat ccgacgact 
      aagaaagtg attccgctc tcgttgctt aattcaaaa 
      ggggggatt aaagtgttg ccaattggg ggtaaaata 
      gtggggaag acaacaacg agcacctcc tcgccccca 
      ttgtacatc gtgaataca gtggccccc aaaaccacg 
      acgcccgca gtggtgaca gaggcgccg cccagtgtg 
      agtaaagaa aatgggaaa tcgtcggtt ttgggcgat 
      attttgaag gcgtctggt gttacaact gacgatttt 
      gatacgcaa attgaggag tttagaatt ccgaataat 
      catgtggct gaggaacaa gtggagcaa gttgaagag 
      gaggaacaa gttacgtcg agtgaaaag ggcgatttt 
      tttattctg cctgaaggt gaagttgtg gaaatgaaa 
      agtgacagt acaaaaaca aacgaagaa caattttca 
      agcgctgat gtcgtgaca gatgatagt gaaacagtg 
      attttgcaa gttcagagt tcggaatta acaagtgtt 
      ggtgatgct gctaactga
    mRNA sequence
    • >TC014793 RA
      ccaatttaa aaacgtaaa gttggcgcc gttcctcga 
      tcaggttgt caaactttc gcaaagaat ttttccaaa 
      ataaaaaca ataaatttg gaaaatagg gaaaactca 
      gcgacggca tttagcaat tttggaaat cacttcaaa 
      tgatgtgaa aattctggt acaggcgag acaaccctt 
      gcgcaaaaa gagtcgcta gtgaacata accacaaaa 
      aataagacg ctgtcatta ctgtaaaaa ttgattttt 
      ttcgtctat tttccactg ggtattgtt aaatatgcc 
      tttgatttg agcaagcaa caatgtggc ccgtgctgt 
      tggatatga caaaagaag aatccatgc aaagtttgc 
      ggaatttag aactggaag cctatgccc atctcgtgt 
      cggcgctgc gagcccaag gccccctta accccgaca 
      aacgcaaac tcctgaagg acaccagcc aggtgctta 
      atatcccac aggaccgcc acaaagccg aaattcgga 
      gagccgtca acgacgaaa aactcaaca cgatcgcat 
      atcatgtta caggcttga ctgaatcgc tggaagatt 
      gggcgcaag aggggcgca ggtccgtgc cgctgctac 
      ccagaatgg ccccccaga ccgcctact ccgccatcg 
      ccgatgagg cggccgaaa acgccaacc agaccaaca 
      aacaactgc cctatcctg ccgccaccg agcgaaaac 
      ggcccccaa tgcagcccc ccgtgccaa cggcgccag 
      aaacgagca agggggcgc agttccgag tcccagaaa 
      cacccaaaa ttgaagaca gcaagaagc ggaaactac 
      ccatttgta cggaaaata gcagtctcg cgcagcacc 
      tcctaggcc ccccgaaga tgagtcgga ctcaacaaa 
      tatacaggc aacggacga aaggggcaa tactgaaag 
      aacagatga agctgaagc cgcagtcaa aaatcccat 
      ttaatccga ttatgcagt cgtcaaacg gccccaaag 
      tagtgttgc ccccaagtg gcgaagaaa caatcgagc 
      agattcaaa acgagataa ataattacc cccaaaaat 
      ctgacgccc caagttgtt ccaatgaga atcaagcac 
      ctcgtgcta aactcttca ctccaacta aacccaccc 
      agagtttaa ctttcaaac aaatcacaa cagcaggca 
      ctagtgacg tgaaattgt gcccaaaaa agaccatca 
      ctaaaccgg ggactcccc tgggacaga aactaatcg 
      ttgtgtcga acgctcaaa cgattcata cctctagta 
      ttctacaaa agactttgt cgataccgt tggttaaaa 
      atatctctg tgaaaaatt tcgaaaagt ttaagattg 
      tcacgacaa gtagcccac agctcgtca ctgccgcca 
      ataatatca attcgttga aacataaag ttgtgactg 
      ttaaaacaa atccgacga ctaagaaag tgattccgc 
      tctcgttgc ttaattcaa aagggggga ttaaagtgt 
      tgccaattg ggggtaaaa tagtgggga agacaacaa 
      cgagcacct cctcgcccc cattgtaca tcgtgaata 
      cagtggccc ccaaaacca cgacgcccg cagtggtga 
      cagaggcgc cgcccagtg tgagtaaag aaaatggga 
      aatcgtcgg ttttgggcg atattttga aggcgtctg 
      gtgttacaa ctgacgatt ttgatacgc aaattgagg 
      agtttagaa ttccgaata atcatgtgg ctgaggaac 
      aagtggagc aagttgaag aggaggaac aagttacgt 
      cgagtgaaa agggcgatt tttttattc tgcctgaag 
      gtgaagttg tggaaatga aaagtgaca gtacaaaaa 
      caaacgaag aacaatttt caagcgctg atgtcgtga 
      cagatgata gtgaaacag tgattttgc aagttcaga 
      gttcggaat taacaagtg ttggtgatg ctgctaact 
      gatatttat ttattttct aatttatta ttattaaat 
      taatccgtt gactttttt taagatgca ttagttcaa 
      ctttttgta cattatcgc tatatttta tcagacgtt 
      ttgctcaat aaatgttca atcactttg tgttgccaa 
      tcaaaattc atgtagcag ttttgcgta ttttagcat 
      tttactgaa aattttgta catatgtaa tagaaaatg 
      attgtagtt aaacaaatt cattgaata tattctgta 
      cgtttaaat aaaccattt ttatttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY