General information | |||
TC014793 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 15136278..15138950 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014793 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05695 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion - Number of animals showing the phenotype: 3 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC014793 RA
MWPVLLDMT KEESMQSLR NLELEAYAH LVSALRAQG PLNPDKRKL LKDTSQVLN IPQDRHKAE IRRAVNDEK LNTIAYHVT GLTESLEDW AQEGRRSVP LLPRMAPQT AYSAIADEA AENANQTNK QLPYPAATE RKRPPMQPP VPTAPETSK GAQFRVPET PKIEDSKKR KLPICTENS SLAQHLLGP PKMSRTQQI YRQRTKGAI LKEQMKLKP QSKIPFNPI MQSSNGPKV VLPPSGEET IEQIQNEIN NYPQKSDAP SCSNENQAP RAKLFTPTK PTQSLTFKQ ITTAGTSDV KLCPKKTIT KPGTPLGQK LIVVSNAQT IHTSSILQK TLSIPLVKN ISVKNFEKF KIVTTSSPQ LVTAANNIN SLKHKVVTV KTNPTTKKV IPLSLLNSK GGIKVLPIG GKIVGKTTT STSSPPLYI VNTVAPKTT TPAVVTEAP PSVSKENGK SSVLGDILK ASGVTTDDF DTQIEEFRI PNNHVAEEQ VEQVEEEEQ VTSSEKGDF FILPEGEVV EMKSDSTKT NEEQFSSAD VVTDDSETV ILQVQSSEL TSVGDAAN
- >TC014793 RA
atgtggccc gtgctgttg gatatgaca aaagaagaa tccatgcaa agtttgcgg aatttagaa ctggaagcc tatgcccat ctcgtgtcg gcgctgcga gcccaaggc ccccttaac cccgacaaa cgcaaactc ctgaaggac accagccag gtgcttaat atcccacag gaccgccac aaagccgaa attcggaga gccgtcaac gacgaaaaa ctcaacacg atcgcatat catgttaca ggcttgact gaatcgctg gaagattgg gcgcaagag gggcgcagg tccgtgccg ctgctaccc agaatggcc ccccagacc gcctactcc gccatcgcc gatgaggcg gccgaaaac gccaaccag accaacaaa caactgccc tatcctgcc gccaccgag cgaaaacgg cccccaatg cagcccccc gtgccaacg gcgccagaa acgagcaag ggggcgcag ttccgagtc ccagaaaca cccaaaatt gaagacagc aagaagcgg aaactaccc atttgtacg gaaaatagc agtctcgcg cagcacctc ctaggcccc ccgaagatg agtcggact caacaaata tacaggcaa cggacgaaa ggggcaata ctgaaagaa cagatgaag ctgaagccg cagtcaaaa atcccattt aatccgatt atgcagtcg tcaaacggc cccaaagta gtgttgccc ccaagtggc gaagaaaca atcgagcag attcaaaac gagataaat aattacccc caaaaatct gacgcccca agttgttcc aatgagaat caagcacct cgtgctaaa ctcttcact ccaactaaa cccacccag agtttaact ttcaaacaa atcacaaca gcaggcact agtgacgtg aaattgtgc ccaaaaaag accatcact aaaccgggg actcccctg ggacagaaa ctaatcgtt gtgtcgaac gctcaaacg attcatacc tctagtatt ctacaaaag actttgtcg ataccgttg gttaaaaat atctctgtg aaaaatttc gaaaagttt aagattgtc acgacaagt agcccacag ctcgtcact gccgccaat aatatcaat tcgttgaaa cataaagtt gtgactgtt aaaacaaat ccgacgact aagaaagtg attccgctc tcgttgctt aattcaaaa ggggggatt aaagtgttg ccaattggg ggtaaaata gtggggaag acaacaacg agcacctcc tcgccccca ttgtacatc gtgaataca gtggccccc aaaaccacg acgcccgca gtggtgaca gaggcgccg cccagtgtg agtaaagaa aatgggaaa tcgtcggtt ttgggcgat attttgaag gcgtctggt gttacaact gacgatttt gatacgcaa attgaggag tttagaatt ccgaataat catgtggct gaggaacaa gtggagcaa gttgaagag gaggaacaa gttacgtcg agtgaaaag ggcgatttt tttattctg cctgaaggt gaagttgtg gaaatgaaa agtgacagt acaaaaaca aacgaagaa caattttca agcgctgat gtcgtgaca gatgatagt gaaacagtg attttgcaa gttcagagt tcggaatta acaagtgtt ggtgatgct gctaactga
- >TC014793 RA
ccaatttaa aaacgtaaa gttggcgcc gttcctcga tcaggttgt caaactttc gcaaagaat ttttccaaa ataaaaaca ataaatttg gaaaatagg gaaaactca gcgacggca tttagcaat tttggaaat cacttcaaa tgatgtgaa aattctggt acaggcgag acaaccctt gcgcaaaaa gagtcgcta gtgaacata accacaaaa aataagacg ctgtcatta ctgtaaaaa ttgattttt ttcgtctat tttccactg ggtattgtt aaatatgcc tttgatttg agcaagcaa caatgtggc ccgtgctgt tggatatga caaaagaag aatccatgc aaagtttgc ggaatttag aactggaag cctatgccc atctcgtgt cggcgctgc gagcccaag gccccctta accccgaca aacgcaaac tcctgaagg acaccagcc aggtgctta atatcccac aggaccgcc acaaagccg aaattcgga gagccgtca acgacgaaa aactcaaca cgatcgcat atcatgtta caggcttga ctgaatcgc tggaagatt gggcgcaag aggggcgca ggtccgtgc cgctgctac ccagaatgg ccccccaga ccgcctact ccgccatcg ccgatgagg cggccgaaa acgccaacc agaccaaca aacaactgc cctatcctg ccgccaccg agcgaaaac ggcccccaa tgcagcccc ccgtgccaa cggcgccag aaacgagca agggggcgc agttccgag tcccagaaa cacccaaaa ttgaagaca gcaagaagc ggaaactac ccatttgta cggaaaata gcagtctcg cgcagcacc tcctaggcc ccccgaaga tgagtcgga ctcaacaaa tatacaggc aacggacga aaggggcaa tactgaaag aacagatga agctgaagc cgcagtcaa aaatcccat ttaatccga ttatgcagt cgtcaaacg gccccaaag tagtgttgc ccccaagtg gcgaagaaa caatcgagc agattcaaa acgagataa ataattacc cccaaaaat ctgacgccc caagttgtt ccaatgaga atcaagcac ctcgtgcta aactcttca ctccaacta aacccaccc agagtttaa ctttcaaac aaatcacaa cagcaggca ctagtgacg tgaaattgt gcccaaaaa agaccatca ctaaaccgg ggactcccc tgggacaga aactaatcg ttgtgtcga acgctcaaa cgattcata cctctagta ttctacaaa agactttgt cgataccgt tggttaaaa atatctctg tgaaaaatt tcgaaaagt ttaagattg tcacgacaa gtagcccac agctcgtca ctgccgcca ataatatca attcgttga aacataaag ttgtgactg ttaaaacaa atccgacga ctaagaaag tgattccgc tctcgttgc ttaattcaa aagggggga ttaaagtgt tgccaattg ggggtaaaa tagtgggga agacaacaa cgagcacct cctcgcccc cattgtaca tcgtgaata cagtggccc ccaaaacca cgacgcccg cagtggtga cagaggcgc cgcccagtg tgagtaaag aaaatggga aatcgtcgg ttttgggcg atattttga aggcgtctg gtgttacaa ctgacgatt ttgatacgc aaattgagg agtttagaa ttccgaata atcatgtgg ctgaggaac aagtggagc aagttgaag aggaggaac aagttacgt cgagtgaaa agggcgatt tttttattc tgcctgaag gtgaagttg tggaaatga aaagtgaca gtacaaaaa caaacgaag aacaatttt caagcgctg atgtcgtga cagatgata gtgaaacag tgattttgc aagttcaga gttcggaat taacaagtg ttggtgatg ctgctaact gatatttat ttattttct aatttatta ttattaaat taatccgtt gactttttt taagatgca ttagttcaa ctttttgta cattatcgc tatatttta tcagacgtt ttgctcaat aaatgttca atcactttg tgttgccaa tcaaaattc atgtagcag ttttgcgta ttttagcat tttactgaa aattttgta catatgtaa tagaaaatg attgtagtt aaacaaatt cattgaata tattctgta cgtttaaat aaaccattt ttatttta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
