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Gene information
General information
TC014791 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15130338..15131751
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014791
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05694

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    eclosion - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs sometimes irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen partially irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: 50% are affected.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014791 RA
      MGDGEPFPS FFFSRAPLP ERVDLITFK EGKKDKFYW 
      MLSIDNDSR NNEPMRHST LAPYDDNDA EMLDCCDLE 
      ENYEINPTK SGKFITSFY VPNALHSVI VGPRGAKLR 
      ELQDNTSTL IKVPKPGED PIVKITGHS ERSVASART 
      QIALLLLSR REKLAITHF VSIPIYSDN IRDNFRKFK 
      DDILKGPAT RGVDETIFQ KVEKFHLTI VTLALLDEK 
      EIDEAKQML NKCQSNVQK IFAGRRPKI VLKGVEIMN 
      DDPHEVDVL YGKVCLDSR EDVDCLQEA ADEILDVFY 
      RNGLVRKQY DNVKLHVTL MNSIFRKNE DNRRESFDA 
      SFILDKYKD YYFGKTALE QIHLSVRFT SAQNGYYQS 
      AAVITL
    CDS sequence
    • >TC014791 RA
      atgggtgac ggcgagcct ttccccagt tttttcttt 
      tcgagggcc cctttgcct gaacgtgtc gacctaatt 
      acctttaaa gagggcaaa aaggataag ttttattgg 
      atgttatca atcgataac gattctaga aataacgag 
      ccgatgagg cactcgaca ctagcccct tatgacgac 
      aatgacgcc gaaatgttg gactgttgc gatttggag 
      gaaaattat gaaatcaac ccgaccaaa tcgggcaaa 
      tttattacg tccttctac gtcccaaat gcgttacat 
      tccgtaatt gtgggcccc cgtggtgct aaattacga 
      gaattgcaa gacaacact agtactttg attaaagta 
      ccaaaacct ggagaggac cccattgtt aaaataacg 
      ggtcatagt gagcgtagc gttgcgtcc gcaaggaca 
      caaattgct ctgctgtta ttatcaaga cgggaaaaa 
      ttggcaatt actcatttc gtctcaatt ccgatttat 
      tccgataat attagagat aatttccgc aagtttaaa 
      gacgacatt ttgaaagga ccagccacg agaggtgtt 
      gatgaaacg attttccaa aaagtggaa aagtttcac 
      ctcacaata gtcacgctg gcgctcctg gatgagaaa 
      gaaattgac gaagctaaa caaatgttg aacaagtgt 
      caaagcaat gttcagaaa attttcgca ggccgaagg 
      cccaaaatt gtgttaaaa ggagttgaa ataatgaat 
      gatgatcct catgaagtt gatgttttg tacggtaaa 
      gtgtgtctg gacagtaga gaggacgtt gattgtctc 
      caagaagct gcagatgaa attttggat gttttttac 
      agaaatggt ttggtgagg aaacaatac gataacgtt 
      aaattgcac gtaactttg atgaactca attttccga 
      aaaaacgaa gacaatcgg agggaatca tttgatgca 
      agttttatc ttagacaag tacaaagac tactatttt 
      gggaaaaca gcactggag caaatacat ctatcagtc 
      aggtttact tcggctcag aatggatat tatcagtca 
      gcggcagtt atcacactg tga
    mRNA sequence
    • >TC014791 RA
      ccggttgaa atcaatcaa agattttaa taacatctt 
      aattgggat cacgaacgg tgttaaaaa ttgacacaa 
      ccagggtta ttcgattcg tagtttaaa attttggcg 
      cacataacc acacatgtc agaatgggt gacggcgag 
      cctttcccc agttttttc ttttcgagg gcccctttg 
      cctgaacgt gtcgaccta attaccttt aaagagggc 
      aaaaaggat aagttttat tggatgtta tcaatcgat 
      aacgattct agaaataac gagccgatg aggcactcg 
      acactagcc ccttatgac gacaatgac gccgaaatg 
      ttggactgt tgcgatttg gaggaaaat tatgaaatc 
      aacccgacc aaatcgggc aaatttatt acgtccttc 
      tacgtccca aatgcgtta cattccgta attgtgggc 
      ccccgtggt gctaaatta cgagaattg caagacaac 
      actagtact ttgattaaa gtaccaaaa cctggagag 
      gaccccatt gttaaaata acgggtcat agtgagcgt 
      agcgttgcg tccgcaagg acacaaatt gctctgctg 
      ttattatca agacgggaa aaattggca attactcat 
      ttcgtctca attccgatt tattccgat aatattaga 
      gataatttc cgcaagttt aaagacgac attttgaaa 
      ggaccagcc acgagaggt gttgatgaa acgattttc 
      caaaaagtg gaaaagttt cacctcaca atagtcacg 
      ctggcgctc ctggatgag aaagaaatt gacgaagct 
      aaacaaatg ttgaacaag tgtcaaagc aatgttcag 
      aaaattttc gcaggccga aggcccaaa attgtgtta 
      aaaggagtt gaaataatg aatgatgat cctcatgaa 
      gttgatgtt ttgtacggt aaagtgtgt ctggacagt 
      agagaggac gttgattgt ctccaagaa gctgcagat 
      gaaattttg gatgttttt tacagaaat ggtttggtg 
      aggaaacaa tacgataac gttaaattg cacgtaact 
      ttgatgaac tcaattttc cgaaaaaac gaagacaat 
      cggagggaa tcatttgat gcaagtttt atcttagac 
      aagtacaaa gactactat tttgggaaa acagcactg 
      gagcaaata catctatca gtcaggttt acttcggct 
      cagaatgga tattatcag tcagcggca gttatcaca 
      ctgtgataa tttttgtat attttattt aaaaataaa 
      ccatttatt ttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY