Status
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Gene information
General information
TC014785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15078498..15081132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014785
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014785 RA
      MTTDTETSP QPKPKRKSR SKSPESRKK RRDDSPHKD 
      SRKRSRRSR SRDRRKSRK YHDLDRPLE EYPRYYGED 
      QEHKKNSDR YWTKYPRKD DHQVGSRYY GGEPDDKDK 
      ESEKETDKS VIAPRERKT VDLLTRTGG AYIPPAKLR 
      MMQAEITDK SSAAYQRIA WEALKKSIH GFINKVNTS 
      NIGIISREL LHENVVRGR GLLCKSIIQ AQAASPTFT 
      NVYAALVAV INSKFPNIG ELLLKRLVL QFKRGFKQN 
      NKIICISAT TFVAHLVNQ RVAHEILAL EILTLLVET 
      PTDDSVEVA ISFLKECGQ KLTEVSSKG INAIFEMLR 
      NILHEGKLE PRIQYMIEV MFQIRKDGF KDHAAVIEE 
      LDLVDEEHQ FTHLITLDD VKQSNAEDI LNVFKFDDN 
      YEENEGKYK TLSKEILDM DSESGSGSE EESGDEEDD 
      EDEDEEEEE GAQKNESNI IDNTETNLV ALRRTIYLT 
      IQSSLDFEE SAHKLMKME LKPGQEIEL CHMFLDCCA 
      EQRTYEKFY GLLAQRFCQ VNQIYVEPF QQIFKDTYA 
      TTHRLDTNK LRNVSKFFA HLLFTDAIG WEVLEIMKL 
      NEEDTNSSN RIFIKILFQ ELAEYMGLE KLNARLKDA 
      TLQPHFEGL FPRDDPKNT RFAINFFTS IGLGGLTAE 
      LREHLKTAP KVNPLTITE KSSDSSSSS TSSSSSSSS 
      DSSDSDSSS SEDEKPRKK KEEKKRKKP TKEASDDDK 
      KRKTDARKH RHNERRSRD RDDRRRRRS PERKHKSRR
    CDS sequence
    • >TC014785 RA
      atgaccacc gacaccgaa acctcccca cagcccaaa 
      cccaagcgt aaaagccgc tccaaatcc cctgaatcc 
      cgtaaaaaa cgtcgtgac gactcgccc cataaagac 
      tcccggaag cgttcccgc cgttcgcgt tcacgagac 
      cgtcgcaaa agtcgcaaa taccacgac ttggaccgc 
      ccccttgaa gaataccct cggtattac ggcgaggat 
      caagaacac aagaaaaac agcgataga tattggacc 
      aaatacccc cgcaaggat gaccatcag gtgggtagt 
      aggtattac gggggcgag cccgacgat aaagacaaa 
      gagtctgag aaggagacg gataagtcg gtgatagcc 
      ccccgtgag cgcaaaacc gtggattta ctgacacgg 
      acaggtggg gcttacatc cctccagcg aagctgcgg 
      atgatgcaa gcggagatt actgataag agctcggcg 
      gcgtatcaa agaattgcg tgggaggcg ttaaaaaaa 
      tccattcat gggtttatt aacaaggtg aatacgtcc 
      aacattggg attatttcg cgggaattg ctccatgag 
      aatgtggta agggggcgc ggactgctc tgcaagtcc 
      ataatacaa gcgcaagct gcgtcccca actttcaca 
      aacgtgtac gcagccctg gtcgctgtc atcaattcg 
      aaattccct aatattgga gaattgtta ctgaaacgt 
      ttggttttg caattcaag cgcggtttc aagcagaat 
      aataaaatt atctgcatt tcggccacg acttttgtg 
      gctcatttg gttaatcag agggttgcg catgaaatt 
      ctcgccttg gagattttg actcttttg gtggagacg 
      ccaactgat gattcggtt gaagttgcg atttcgttt 
      ctgaaggaa tgtggacag aaattgact gaggtttcg 
      agtaaaggc attaatgcc atctttgaa atgttgagg 
      aatatttta catgagggg aagttggaa ccgaggatt 
      cagtacatg attgaagtt atgttccaa atcaggaag 
      gacgggttt aaggatcat gccgctgtt attgaagag 
      ttggatttg gtggatgag gagcaccag tttacacat 
      ttgattacg ctggatgat gttaagcaa tcaaatgct 
      gaggatatt ttaaatgtg tttaaattc gacgacaac 
      tacgaggaa aacgagggg aagtacaag actttgagc 
      aaggaaatc ctggacatg gacagcgag tcgggctcc 
      ggctcggag gaagagtcc ggtgacgag gaagacgac 
      gaagacgag gacgaagag gaagaagaa ggcgcccaa 
      aagaacgaa tccaacata atcgacaat acggagacc 
      aacctcgtg gccctacgc agaacaatc tacttaacc 
      atccaatca agtttagat tttgaggaa agtgcccac 
      aaactaatg aaaatggaa ctgaaaccg ggccaagaa 
      atcgaatta tgccacatg ttcctcgac tgttgtgcc 
      gaacaacgg acttacgag aagttttac ggattattg 
      gcacagaga ttttgtcaa gtgaatcag atttatgtc 
      gagccattc caacaaatc ttcaaagac acgtacgcc 
      acaacacac agacttgac acaaataaa ttgcgaaat 
      gttagcaag tttttcgca catttgttg ttcaccgat 
      gcgattgga tgggaggtt ttagaaatt atgaaactg 
      aacgaagag gataccaat agttccaat cggattttc 
      atcaagatt ttgttccag gaattggcc gaatatatg 
      ggcttggag aagttaaat gccaggctt aaagatgca 
      acgcttcag ccacatttt gaggggctg tttccgcgc 
      gatgatccc aaaaataca agatttgcg attaatttt 
      ttcacgtcg attggctta ggggggctt acggccgag 
      ttgagggaa catttgaaa acagcccct aaagtcaat 
      cccttgacg attactgaa aaatcaagt gattctagc 
      agcagtagc actagcagt tcaagctca tccagttcc 
      gatagtagt gattcggat tcgtcaagt tccgaagat 
      gagaaacca cgtaagaag aaggaagaa aagaagagg 
      aaaaaaccg acaaaagaa gcatcagac gatgataaa 
      aaacgaaaa acagatgcg aggaaacac agacataat 
      gagagacgg tcacgcgat cgagacgat cggaggcgg 
      cgtaggagc cccgagagg aagcataag agtaggaga 
      taa
    mRNA sequence
    • >TC014785 RA
      cacataacc tcaacctct tgcgttgtt gtgtgttgt 
      caataatcg ccccaattt cttactgtg tttttgtga 
      gtgcttaaa aaatgacca ccgacaccg aaacctccc 
      cacagccca aacccaagc gtaaaagcc gctccaaat 
      cccctgaat cccgtaaaa aacgtcgtg acgactcgc 
      cccataaag actcccgga agcgttccc gccgttcgc 
      gttcacgag accgtcgca aaagtcgca aataccacg 
      acttggacc gcccccttg aagaatacc ctcggtatt 
      acggcgagg atcaagaac acaagaaaa acagcgata 
      gatattgga ccaaatacc cccgcaagg atgaccatc 
      aggtgggta gtaggtatt acgggggcg agcccgacg 
      ataaagaca aagagtctg agaaggaga cggataagt 
      cggtgatag ccccccgtg agcgcaaaa ccgtggatt 
      tactgacac ggacaggtg gggcttaca tccctccag 
      cgaagctgc ggatgatgc aagcggaga ttactgata 
      agagctcgg cggcgtatc aaagaattg cgtgggagg 
      cgttaaaaa aatccattc atgggttta ttaacaagg 
      tgaatacgt ccaacattg ggattattt cgcgggaat 
      tgctccatg agaatgtgg taagggggc gcggactgc 
      tctgcaagt ccataatac aagcgcaag ctgcgtccc 
      caactttca caaacgtgt acgcagccc tggtcgctg 
      tcatcaatt cgaaattcc ctaatattg gagaattgt 
      tactgaaac gtttggttt tgcaattca agcgcggtt 
      tcaagcaga ataataaaa ttatctgca tttcggcca 
      cgacttttg tggctcatt tggttaatc agagggttg 
      cgcatgaaa ttctcgcct tggagattt tgactcttt 
      tggtggaga cgccaactg atgattcgg ttgaagttg 
      cgatttcgt ttctgaagg aatgtggac agaaattga 
      ctgaggttt cgagtaaag gcattaatg ccatctttg 
      aaatgttga ggaatattt tacatgagg ggaagttgg 
      aaccgagga ttcagtaca tgattgaag ttatgttcc 
      aaatcagga aggacgggt ttaaggatc atgccgctg 
      ttattgaag agttggatt tggtggatg aggagcacc 
      agtttacac atttgatta cgctggatg atgttaagc 
      aatcaaatg ctgaggata ttttaaatg tgtttaaat 
      tcgacgaca actacgagg aaaacgagg ggaagtaca 
      agactttga gcaaggaaa tcctggaca tggacagcg 
      agtcgggct ccggctcgg aggaagagt ccggtgacg 
      aggaagacg acgaagacg aggacgaag aggaagaag 
      aaggcgccc aaaagaacg aatccaaca taatcgaca 
      atacggaga ccaacctcg tggccctac gcagaacaa 
      tctacttaa ccatccaat caagtttag attttgagg 
      aaagtgccc acaaactaa tgaaaatgg aactgaaac 
      cgggccaag aaatcgaat tatgccaca tgttcctcg 
      actgttgtg ccgaacaac ggacttacg agaagtttt 
      acggattat tggcacaga gattttgtc aagtgaatc 
      agatttatg tcgagccat tccaacaaa tcttcaaag 
      acacgtacg ccacaacac acagacttg acacaaata 
      aattgcgaa atgttagca agtttttcg cacatttgt 
      tgttcaccg atgcgattg gatgggagg ttttagaaa 
      ttatgaaac tgaacgaag aggatacca atagttcca 
      atcggattt tcatcaaga ttttgttcc aggaattgg 
      ccgaatata tgggcttgg agaagttaa atgccaggc 
      ttaaagatg caacgcttc agccacatt ttgaggggc 
      tgtttccgc gcgatgatc ccaaaaata caagatttg 
      cgattaatt ttttcacgt cgattggct taggggggc 
      ttacggccg agttgaggg aacatttga aaacagccc 
      ctaaagtca atcccttga cgattactg aaaaatcaa 
      gtgattcta gcagcagta gcactagca gttcaagct 
      catccagtt ccgatagta gtgattcgg attcgtcaa 
      gttccgaag atgagaaac cacgtaaga agaaggaag 
      aaaagaaga ggaaaaaac cgacaaaag aagcatcag 
      acgatgata aaaaacgaa aaacagatg cgaggaaac 
      acagacata atgagagac ggtcacgcg atcgagacg 
      atcggaggc ggcgtagga gccccgaga ggaagcata 
      agagtagga gataagtta ctttgtaca taaattgta 
      ttgaataaa tatttttat ttgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY