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Gene information
General information
TC014780 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15013135..15014244
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014780
    iBeetle screen iB_05690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014780 RA
      MKVLAVFDF DHTILDDNS DTAVIALTD KDNLPVEIR 
      SLHSTEGWT AFMQAIFKL LYHEGRTID EINNLITNL 
      KPVGGMVPL IEEFHKNPA FELIIISDS NAHFIKIWL 
      EKNNVSKCF TKVFTNPSK IENGLLTIS PYHNQNACK 
      LSTNNLCKG TVLDEFVQE QRDKGVVYD RIVYIGDGV 
      NDFCPVLRL HKSDLACVR KGFKLQDLV TKAQKGTSF 
      DNSGKPHVI LAEIFVWET GDDILKHLQ LK
    CDS sequence
    • >TC014780 RA
      atgaaagtc ctggcagtg tttgatttt gaccacacg 
      atcctcgac gacaactcc gacacagct gtcatagcc 
      ctaactgac aaagacaac cttcctgtt gaaatccga 
      agtctccac tccactgaa ggctggacg gcttttatg 
      caagcaatt ttcaaactt ttgtaccac gaaggtcgg 
      acaatcgat gaaataaac aatctaata acgaacttg 
      aagcctgtg gggggcatg gtgcccctc atcgaggag 
      tttcacaaa aacccagct tttgaactg attatcatt 
      agtgattct aacgcccat tttataaag atttggtta 
      gaaaaaaac aacgttagc aagtgtttc actaaagtg 
      ttcacaaac ccatcgaaa atcgaaaat ggattactg 
      accatttcc ccttaccat aatcaaaat gcgtgcaaa 
      ctcagcacg aataatttg tgtaaaggg acagtgctg 
      gatgagttt gtccaggaa caacgtgac aagggggtt 
      gtctacgac aggattgtg tacataggg gatggggtg 
      aacgacttt tgccccgtt ttgcgcctc cacaagtcc 
      gaccttgcg tgtgtgaga aaaggcttc aagttgcaa 
      gacctcgtc accaaggcg caaaaagga acgtcgttt 
      gataattca gggaagccc cacgttatc ctcgctgag 
      attttcgtt tgggagacg ggggacgat attttgaag 
      cacctccag ttgaaatag
    mRNA sequence
    • >TC014780 RA
      aatgtcatc aataatccg gactgtaac aaatgcttg 
      tagaataaa tatttcgca aaaacatct aacagtttc 
      acacaaaaa tcgtcaaaa agccacatt ccaactttt 
      ccgtagcac tgttcgccc aattttccc tccttcaca 
      ccaacttaa aatgaaagt cctggcagt gtttgattt 
      tgaccacac gatcctcga cgacaactc cgacacagc 
      tgtcatagc cctaactga caaagacaa ccttcctgt 
      tgaaatccg aagtctcca ctccactga aggctggac 
      ggcttttat gcaagcaat tttcaaact tttgtacca 
      cgaaggtcg gacaatcga tgaaataaa caatctaat 
      aacgaactt gaagcctgt ggggggcat ggtgcccct 
      catcgagga gtttcacaa aaacccagc ttttgaact 
      gattatcat tagtgattc taacgccca ttttataaa 
      gatttggtt agaaaaaaa caacgttag caagtgttt 
      cactaaagt gttcacaaa cccatcgaa aatcgaaaa 
      tggattact gaccatttc cccttacca taatcaaaa 
      tgcgtgcaa actcagcac gaataattt gtgtaaagg 
      gacagtgct ggatgagtt tgtccagga acaacgtga 
      caagggggt tgtctacga caggattgt gtacatagg 
      ggatggggt gaacgactt ttgccccgt tttgcgcct 
      ccacaagtc cgaccttgc gtgtgtgag aaaaggctt 
      caagttgca agacctcgt caccaaggc gcaaaaagg 
      aacgtcgtt tgataattc agggaagcc ccacgttat 
      cctcgctga gattttcgt ttgggagac gggggacga 
      tattttgaa gcacctcca gttgaaata gcggactcg 
      catcggtat taattactt tattcagtt acaacttta 
      attacattg gcacctaat gcttagcag ccaccacgt 
      ttcattcaa caaccaaaa ttatttaca tttaaatag 
      aaatttgct taattctat tgtgactat gttgcgaat 
      aaattatca aaagatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY