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Gene information
General information
TC014775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14912327..14922261
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014775
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05689

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014775 RA
      MDITKEYYN PNPREKANF LSVMFFTYT IDMFKKGYS 
      KTLDVDDLY SPIESDRST LLGDRLESK WNKHMDSIK 
      KSKKKRKPS LLKVLVATF WPEYLLLGV ILVIMDLCV 
      RLSQPIMLG RLLDHFKPN SDVDKTAAL WYAGAIVGL 
      NAMSALLIN QYIMRAFHY GMKVRAACC ALIYRKALR 
      LSKTALADT AAGKVVNLL SNDVSRFDV VSVLIHHMW 
      VAPTSAIIV AYLLYDAAG YAGLIGIAP VFLVVPLQS 
      YTGKLSAIY RKKTAQKTD ERVRLMDEI ISGVQVIKM 
      YAWEIPFAK LIRFARKAE LRIVMKSSY VRGLYMTFN 
      LFTTRAALF CTLLCLALT KQDITASRV FVFMSYYNI 
      LAQTMSSMF VRGISEMAE VLVAITRLQ SFMTNEEFV 
      KVKVGDNND NKINSDKTR LALRNVCAK WDVSSSDNT 
      LSNVNFSIN QRKLLAVIG PVGSGKSSL LQAILGELE 
      VTSGDLHIN GSISYASQD PWVFAATVR QNITFGLDF 
      NKKRYNEVV HACALEKDF KQFPDGDLT IVGDRGASL 
      SGGQKARIN LARAVYRDA DIYLLDDPL SAVDIHVSK 
      HLYDECING YLANKTRIL VTHQVHHLK NADEIIILN 
      NGAIENKGT FENLANSDT IYARLLTTE PEHTAEEKQ 
      KMFEAAKLT RQISTRSKT SSLASAVSD LSIPESILQ 
      EEAQEPEIK VKSLQEESS KGKVHGSLF WQYLLAGGN 
      FLFVSVVLI LYVLAQAAA SGVDFFVSF WVNIEEARN 
      STSNITAIG EAPDWSTET CLYIYGGFI AAVFIIAFT 
      RSMLFYKLA MLSSQKLHD TLFNCVINA SMKFFDTNP 
      SGRILNRFS KDIGAIDEL LPKAILDAG QIILNMAGA 
      LILVAIVNP YFLIIVGIT GIFFMLLRV VFLRSSKNI 
      KRLEGMMRS PVFTHLNAT LNGLTTIRA FQAETILRN 
      EFDKHQDYH TSAWFMYIA ASSAFGFYL DFLCFIFVA 
      LVTFSFLTF GDSIGLKGG EVGLAITQS AALTGLVQW 
      GMRQSAEVA NQLMSVERV LEYKELPKE KQPQQPKTP 
      PKSWPANGK IAFTDMGLK YDEKAPLVL KDLNIVINP 
      KEKVGIVGR TGAGKSSLI AALFRLANV IGEIDIDDI 
      DTKHLQLQV LRSKISIIP QDPVLFSGT LRYNLDPFE 
      DYPDEVLYK ALNEVELKD PANIINRLE NRVMDRGSN 
      YSVGQRQLI CLARAIIRN NKILMLDEA TANVDPQTD 
      ALIQKTIRQ KFADCTVLT VAHRLNTIM DSDKVLVME 
      SGTMVEFDH PHVLLQNPS SKFSKMVAE TGKSTAEQL 
      RKIARSSYL KKHAVPE
    CDS sequence
    • >TC014775 RA
      atggatatc accaaagag tattacaac ccgaatccc 
      cgggagaaa gcgaatttt ctctcggtg atgtttttc 
      acttacacg attgacatg ttcaaaaaa ggctacagt 
      aagaccctt gacgttgat gacctttac agccccatc 
      gaaagtgac agaagtacc ctattgggc gacagactc 
      gagagcaaa tggaacaag cacatggac agcataaaa 
      aaatcaaag aagaagcgc aaacccagc ttgctcaaa 
      gtgctcgtg gccaccttc tggcccgag tatctcctc 
      ctaggtgtt attttagtc ataatggat ttatgcgtg 
      agattaagc cagcctatc atgttggga cgactcctg 
      gaccatttc aaacccaac agcgatgtt gataagacc 
      gctgctctg tggtacgct ggagctatt gtgggatta 
      aatgccatg agtgcgctg ctcattaat cagtacatc 
      atgagggca ttccattac gggatgaag gttcgagcc 
      gcttgctgc gctcttatt tacagaaag gcccttcgc 
      ctgagcaag acggcgtta gctgacacc gctgccggc 
      aaagtagta aatctcctc tcgaacgat gtaagccgt 
      ttcgacgtc gtttccgtc ctgatccac cacatgtgg 
      gtggcacca acgtcggct ataatcgta gcttacctt 
      ctttacgac gcagctgga tatgctggc cttattgga 
      atagctcca gtcttcctc gtagtgcct ttgcaaagt 
      tacactggc aaactctcc gcaatttac cgaaagaaa 
      acggcgcaa aaaaccgac gaaagggtg cgccttatg 
      gacgaaatc atctccgga gtccaagtg atcaaaatg 
      tacgcctgg gagatacct ttcgccaaa ctgatccgt 
      tttgcccga aaggccgag cttagaatc gtgatgaaa 
      tcgtcctac gtgcgggga ctttacatg acgtttaac 
      cttttcaca acccgagct gcgcttttt tgtacctta 
      ctttgcctg gctttgacc aaacaggac ataaccgca 
      agtcgtgtt ttcgtcttc atgtcgtac tacaatatt 
      ttggcccaa actatgtcc agtatgttc gtacgtggc 
      atctccgag atggccgaa gtcctggtc gccatcacg 
      cgtctccag agcttcatg acgaacgaa gagttcgtc 
      aaagttaaa gtcggggac aataatgat aataaaatc 
      aactctgac aagacccgc ctcgctttg cgcaacgtt 
      tgtgccaaa tgggacgtt tcctcctct gataacacc 
      ctgtccaac gtcaatttc agcataaat cagaggaaa 
      ttactggcg gtgattggg cctgttggc agcggcaaa 
      agctcgctg ctgcaggcg attctgggc gagttggag 
      gtcacctca ggcgatctg cacatcaac ggatcgatt 
      tcctacgcg tcgcaagac ccttgggtg tttgccgcc 
      accgtccgg caaaacatc accttcggg ctcgatttt 
      aacaagaag aggtacaac gaagtcgtg catgcttgt 
      gccttggag aaggacttt aagcagttt cccgacggc 
      gatttgacc attgttggg gatcgcggc gcctcgttg 
      agtggcgga cagaaggcg aggattaat ttggcacgt 
      gctgtttac cgagatgct gacatttac ttactcgac 
      gatccttta tcggctgtt gatatccat gtgtctaag 
      catctttac gatgaatgc atcaatggc tatctggcg 
      aacaagacg cggatcctc gtcacacat caagttcac 
      cacttgaaa aatgccgac gagatcatc atcctaaat 
      aatggcgcc atcgagaac aaaggcacc tttgaaaac 
      ctagccaac agtgacacc atatacgcg agactgctg 
      acaacagaa cccgaacac acggctgaa gaaaaacag 
      aaaatgttc gaagcggcg aaattaacc cgccaaatc 
      tcgactcga agcaaaacc tcctcgttg gcaagcgcc 
      gttagcgac ttaagcatc cccgaaagc atcctccag 
      gaggaggcg caagaaccc gaaatcaaa gtcaagagt 
      ttgcaggag gaatcctcc aaaggcaaa gtgcacggt 
      tccctcttc tggcagtat ttactagcc ggaggcaac 
      ttcctcttc gtctcagtt gtcctgatt ctgtacgtc 
      ctggcccaa gctgctgcc agtggtgtc gatttcttc 
      gtcagtttt tgggtcaat atcgaagaa gcgaggaat 
      agtacgagt aacataact gcgattggc gaagccccg 
      gattggtca accgagact tgcctctac atctacggt 
      ggatttatc gcagctgtg tttattatt gccttcacc 
      aggtctatg ctgttctac aagctggca atgctaagt 
      tcccagaaa ctgcacgac actttgttc aattgtgtg 
      ataaatgca tcgatgaaa ttctttgat actaacccc 
      agtggtcgc attttgaac cgcttttcc aaagatata 
      ggggccata gatgaactg ctgccaaag gcaatacta 
      gatgccggc caaattatc ctaaacatg gccggtgcc 
      ttaattttg gtagctata gtaaacccg tattttttg 
      ataatagtt ggcatcaca ggaatcttc ttcatgtta 
      ctaagagtc gtgtttctg cgatcgtcg aaaaatatc 
      aaacgactc gaaggaatg atgcgaagt cccgtcttc 
      acgcacttg aacgcaacc ctaaacggt ttgaccaca 
      attcgggca ttccaagcc gaaaccatc cttcgaaac 
      gaattcgac aaacaccaa gactaccac actagtgct 
      tggttcatg tatattgcg gcaagttcg gctttcggc 
      ttctatttg gacttcctc tgtttcatt tttgtggca 
      ttggtcact ttcagcttc ctcacattt ggcgatagt 
      attggacta aaagggggt gaagtagga ctagccata 
      acccagtca gctgccctc acaggtcta gtccaatgg 
      ggcatgcgc caatcagcc gaagtcgcc aatcagtta 
      atgtcagtg gaacgagtc ctcgaatac aaagaactg 
      cccaaagaa aaacaaccc caacagccc aaaacaccc 
      ccgaaatca tggccagct aacgggaaa atcgccttc 
      acagacatg ggcttgaaa tatgacgag aaggcgccc 
      ttggtgctc aaagacctg aacattgtt attaacccc 
      aaggaaaaa gtcgggatt gttgggcgc acaggtgcc 
      gggaaatcg tccctgatt gcggccctt tttcgccta 
      gccaacgtc attggggag attgacatt gacgatatc 
      gacaccaaa cacttgcaa ctgcaagtt ttgaggtcc 
      aagatttcg attatcccc caggatccc gtcctgttt 
      tcggggact ttgcgctac aacctggac ccctttgag 
      gattatccc gacgaagtc ctgtacaaa gctttgaac 
      gaagtggag ctcaaagac ccggcaaac attattaac 
      cggttggag aaccgtgtc atggatcga ggatcgaac 
      tacagtgtg ggccagcga cagttgatt tgtcttgcc 
      agggccata attaggaat aataagatt ttaatgctg 
      gatgaggca actgccaat gtggaccca caaactgac 
      gcactcatc cagaagaca atccggcaa aagttcgcc 
      gattgtaca gttttgacg gtcgcccat cgtctcaat 
      accatcatg gactccgac aaggttcta gtgatggag 
      agcggtacc atggtcgag ttcgaccac ccccacgtc 
      ctcctccag aacccgtca agcaaattt agcaaaatg 
      gtcgcagag acgggaaaa tcaacggcg gaacaactc 
      cggaaaatt gcgagaagt agttatttg aagaagcac 
      gccgtccct gaatga
    mRNA sequence
    • >TC014775 RA
      gtcttcacg gaaggacgt ggggaatgc cgcggtgaa 
      gtcgcgtgt gttagtttt ttaaaaaat ttcattacg 
      aattaaagt acagtattc ccggtgcca tccatgcga 
      aagttctaa tttcgacat aaagcagtt aaaataaat 
      aaacaccgt gtatgtgct accagaaaa ataaaccaa 
      gtgtgcagt gttacaaac accggtcat ggatatcac 
      caaagagta ttacaaccc gaatccccg ggagaaagc 
      gaattttct ctcggtgat gtttttcac ttacacgat 
      tgacatgtt caaaaaagg ctacagtaa gacccttga 
      cgttgatga cctttacag ccccatcga aagtgacag 
      aagtaccct attgggcga cagactcga gagcaaatg 
      gaacaagca catggacag cataaaaaa atcaaagaa 
      gaagcgcaa acccagctt gctcaaagt gctcgtggc 
      caccttctg gcccgagta tctcctcct aggtgttat 
      tttagtcat aatggattt atgcgtgag attaagcca 
      gcctatcat gttgggacg actcctgga ccatttcaa 
      acccaacag cgatgttga taagaccgc tgctctgtg 
      gtacgctgg agctattgt gggattaaa tgccatgag 
      tgcgctgct cattaatca gtacatcat gagggcatt 
      ccattacgg gatgaaggt tcgagccgc ttgctgcgc 
      tcttattta cagaaaggc ccttcgcct gagcaagac 
      ggcgttagc tgacaccgc tgccggcaa agtagtaaa 
      tctcctctc gaacgatgt aagccgttt cgacgtcgt 
      ttccgtcct gatccacca catgtgggt ggcaccaac 
      gtcggctat aatcgtagc ttaccttct ttacgacgc 
      agctggata tgctggcct tattggaat agctccagt 
      cttcctcgt agtgccttt gcaaagtta cactggcaa 
      actctccgc aatttaccg aaagaaaac ggcgcaaaa 
      aaccgacga aagggtgcg ccttatgga cgaaatcat 
      ctccggagt ccaagtgat caaaatgta cgcctggga 
      gataccttt cgccaaact gatccgttt tgcccgaaa 
      ggccgagct tagaatcgt gatgaaatc gtcctacgt 
      gcggggact ttacatgac gtttaacct tttcacaac 
      ccgagctgc gcttttttg taccttact ttgcctggc 
      tttgaccaa acaggacat aaccgcaag tcgtgtttt 
      cgtcttcat gtcgtacta caatatttt ggcccaaac 
      tatgtccag tatgttcgt acgtggcat ctccgagat 
      ggccgaagt cctggtcgc catcacgcg tctccagag 
      cttcatgac gaacgaaga gttcgtcaa agttaaagt 
      cggggacaa taatgataa taaaatcaa ctctgacaa 
      gacccgcct cgctttgcg caacgtttg tgccaaatg 
      ggacgtttc ctcctctga taacaccct gtccaacgt 
      caatttcag cataaatca gaggaaatt actggcggt 
      gattgggcc tgttggcag cggcaaaag ctcgctgct 
      gcaggcgat tctgggcga gttggaggt cacctcagg 
      cgatctgca catcaacgg atcgatttc ctacgcgtc 
      gcaagaccc ttgggtgtt tgccgccac cgtccggca 
      aaacatcac cttcgggct cgattttaa caagaagag 
      gtacaacga agtcgtgca tgcttgtgc cttggagaa 
      ggactttaa gcagtttcc cgacggcga tttgaccat 
      tgttgggga tcgcggcgc ctcgttgag tggcggaca 
      gaaggcgag gattaattt ggcacgtgc tgtttaccg 
      agatgctga catttactt actcgacga tcctttatc 
      ggctgttga tatccatgt gtctaagca tctttacga 
      tgaatgcat caatggcta tctggcgaa caagacgcg 
      gatcctcgt cacacatca agttcacca cttgaaaaa 
      tgccgacga gatcatcat cctaaataa tggcgccat 
      cgagaacaa aggcacctt tgaaaacct agccaacag 
      tgacaccat atacgcgag actgctgac aacagaacc 
      cgaacacac ggctgaaga aaaacagaa aatgttcga 
      agcggcgaa attaacccg ccaaatctc gactcgaag 
      caaaacctc ctcgttggc aagcgccgt tagcgactt 
      aagcatccc cgaaagcat cctccagga ggaggcgca 
      agaacccga aatcaaagt caagagttt gcaggagga 
      atcctccaa aggcaaagt gcacggttc cctcttctg 
      gcagtattt actagccgg aggcaactt cctcttcgt 
      ctcagttgt cctgattct gtacgtcct ggcccaagc 
      tgctgccag tggtgtcga tttcttcgt cagtttttg 
      ggtcaatat cgaagaagc gaggaatag tacgagtaa 
      cataactgc gattggcga agccccgga ttggtcaac 
      cgagacttg cctctacat ctacggtgg atttatcgc 
      agctgtgtt tattattgc cttcaccag gtctatgct 
      gttctacaa gctggcaat gctaagttc ccagaaact 
      gcacgacac tttgttcaa ttgtgtgat aaatgcatc 
      gatgaaatt ctttgatac taaccccag tggtcgcat 
      tttgaaccg cttttccaa agatatagg ggccataga 
      tgaactgct gccaaaggc aatactaga tgccggcca 
      aattatcct aaacatggc cggtgcctt aattttggt 
      agctatagt aaacccgta ttttttgat aatagttgg 
      catcacagg aatcttctt catgttact aagagtcgt 
      gtttctgcg atcgtcgaa aaatatcaa acgactcga 
      aggaatgat gcgaagtcc cgtcttcac gcacttgaa 
      cgcaaccct aaacggttt gaccacaat tcgggcatt 
      ccaagccga aaccatcct tcgaaacga attcgacaa 
      acaccaaga ctaccacac tagtgcttg gttcatgta 
      tattgcggc aagttcggc tttcggctt ctatttgga 
      cttcctctg tttcatttt tgtggcatt ggtcacttt 
      cagcttcct cacatttgg cgatagtat tggactaaa 
      agggggtga agtaggact agccataac ccagtcagc 
      tgccctcac aggtctagt ccaatgggg catgcgcca 
      atcagccga agtcgccaa tcagttaat gtcagtgga 
      acgagtcct cgaatacaa agaactgcc caaagaaaa 
      acaacccca acagcccaa aacaccccc gaaatcatg 
      gccagctaa cgggaaaat cgccttcac agacatggg 
      cttgaaata tgacgagaa ggcgccctt ggtgctcaa 
      agacctgaa cattgttat taaccccaa ggaaaaagt 
      cgggattgt tgggcgcac aggtgccgg gaaatcgtc 
      cctgattgc ggccctttt tcgcctagc caacgtcat 
      tggggagat tgacattga cgatatcga caccaaaca 
      cttgcaact gcaagtttt gaggtccaa gatttcgat 
      tatccccca ggatcccgt cctgttttc ggggacttt 
      gcgctacaa cctggaccc ctttgagga ttatcccga 
      cgaagtcct gtacaaagc tttgaacga agtggagct 
      caaagaccc ggcaaacat tattaaccg gttggagaa 
      ccgtgtcat ggatcgagg atcgaacta cagtgtggg 
      ccagcgaca gttgatttg tcttgccag ggccataat 
      taggaataa taagatttt aatgctgga tgaggcaac 
      tgccaatgt ggacccaca aactgacgc actcatcca 
      gaagacaat ccggcaaaa gttcgccga ttgtacagt 
      tttgacggt cgcccatcg tctcaatac catcatgga 
      ctccgacaa ggttctagt gatggagag cggtaccat 
      ggtcgagtt cgaccaccc ccacgtcct cctccagaa 
      cccgtcaag caaatttag caaaatggt cgcagagac 
      gggaaaatc aacggcgga acaactccg gaaaattgc 
      gagaagtag ttatttgaa gaagcacgc cgtccctga 
      atgatctgt tgcattttt acttaatcg ctagtattt 
      tatgtgttc ctgtactgt attacttaa ggcccagtt 
      gatatttta agttactta aattagggt aataaggct 
      gtgttcctt gccttgtgt aaaccaaag cggcttcaa 
      atcggtgcc aaacgtggt gctcttatc aaaatgtaa 
      ataaatatt ttaaatata t
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