| General information | |||
| TC014769 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 14836757..14845318 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC014769 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05687 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC014769 RA
MKKNFAIGP RSSSEPSLA QILLHSAEL AKPETLTSV QSNDNQNLK SRVSDLETT LAQKEQTVE ILREKLDLA MKDKQILKD SIVKITKNC VCALKNKIM DEATADLTT KLEEKERQI HDLQEERTV LSREVQDCE NYISNQVDQ MKKLASYID RLTQNSEKL QVERKSKTS TVSNLNGEN CSCDSKRIK ELEARINRY KARLNSKDE SIKSLQEQL EFERISREK LEEAFLRTA KIYEDLVHQ NQGLGNMKY EYEACKAQC EQFRATCED LRFKARKVE ELEREINHC NCYMVNQDN RITQLLEDI EVMAQRENE MKITILNME TTLEEYNEK FEKCQNEKA SLKDRIEAL ETQIEDYDN IVALKEETN KTLADNLEK EKAAKAKLE SSLDTIVKT DKHLKEMLT QARQAMKTI TQRLEECDI ERLETQRDN QMKISELEN ELILANATI KKLKENLDQ MKGVMDKNV ALRTLSAQL HESANLQVA QLKSANEDL SRRSQKMVE MYDYLKQVL YEKDKTFED LRNHLVSLR QRLDNEQKL REDTENILA DVAATGDSE LSELRNVIS VLKNEICCL HCELEDLMN TTTAGRLKT RVKELEVEV EVLKCERDK YRKYLEKVL PGYVDPEEL ERLHNELNQ MTEETARLN EELQRQKKL RIDTVTGLQ MKIEKQEKE NKMIKEQIR ESIEQKKRR DPTLPNVVF VEKKDKEDG GVEYRYLYG IAEEKKKSI QPPQQDYYK YECYTVENG IEKRVFLGP LHLTYIDGD GEVQYFSID PKTATPRKE FYVKERGQN KKILWNLKT GPFKLNIEE EVSLKQKYE DATREINRL QSRLEFVKT ETSGLGEKL IKDARDIND IDSKITSAI SEAVTLQKQ LDMPRSRSQ ESFSQERET LTREIEKQK REIADLLRK IQDSSSVGH DALKELESK KEENEKLKT MVRDLERQL QNAQFKVEI PKSSPQVDT LVENLKHHL EKEQKRVNE LEDLLHTCQ KNEKEVKKE NANLKSEIT TLKRDKRMS TASHLFVNQ LQQVDDNLK DWQNQINNN VSHVQKQVN DIIDKLEGA PGNTDVVQK LLEGIKHFE ADLVDNVVN KKRVALTND LQEKQINEL EKQLSDLRQ IIHNLEEEK ENAKKLAPT QQLEEQLRE YQRKNADLN RELEQLRKS QRDARRSID SDFRHKRIS EVISKDSLT DKDARVKEL EDTLHQWQR DYDLEVGKL KKEIEKLMM RGNLPQGCE AHETLIREL KNDLEKSQR KNENYERKI AHLEGENEN LLTMMDKYE GSIDSAADD KKVRDLVEH LRELQRKNE ALKTRTIQL ELEHGRMKN DLDRLVGQE NKSYEKEMQ RLRDLERQS ARSQDKYQN LKKRLMKLE IENKNLKKE YIDTLIAKE KISLKSREQ EQRLRETNK LLRKCQDKK QTLTMRMLK AQVDHDDLK KQLEIDSQN IENENCMKI QNINKELII RNAQLEEEN NYLKNLIPE EERAKVAQI ESQMVDVDE ELKKFNEQA EFKARIAHL EMENALMKK ELEAVKAKD VSSFEYETE KRSADNLQI RAVELEIEN EFLRSELTD REKISYDNG INERKINEL EEEIQRLNE ANEKLQMKN VDLELDHQY MKDELDRIS KVRQKLESE IPSKDVEKQ NHSLKLKND HLELEIKKL KVQLENMGK EQISLQSHI QEKRLKEME ALINEYETM RHTPVERTL KRENLDNVE EVEEKMQEW QEQFRPQLL DMKKDLEKL MGKSRSGTN LKQIEEQLR EAKTKVAHL ETENRNLKV ENEIIRKGI PANEAKLVR DVEQILKNL PKPEFAPKS VDQKKLKDL EARLRECQQ VNDHLRNEK IALDKHVQS KRIQDLETQ IREHRFRDS SSKTDLRLR GQKSIMLDF QDMRVKQLE DELGACQNR NEALKAELE TLRRESVAT NSDASTKFQ ECQKQNLLL KDELERLRK INDLQQKLA KDLDKQLRE CKMNLEECQ RDVKSSKDR KVSFHNIHV NVNDLTTEE LVAELERLS NECEQQRRM IHELEESLR KCRENNREF KQENDELKI ERQFMRQEY GVPKDERLR QLRDEQKRK TGGTSDDVS RATDDISTL QAKLELSEN DNKKLKDEL NTLTQERDN LLSEKCELQ QKIQRMSGD YEAVLEEKE KLNLVISDL EKKIEDLTK LQLEVGKGD TDDKRSRKL EETLQKYHD NNENLKIRI VHLEEEKTN LKKELSARI EKEKKTPGS EQQARQIRK LEQTLQKYH DNNENLKRK IAFLEADYQ EKRVVELEE QVQKCQINN DGLKQVTVQ LESEIKYLK EELGTFLQK EKKVTRKRQ TTASEKNKP KQSLFMQPP VYAMPVFIQ PPFVSYGNY LAPQVASTG TGGDIEQLS HLVDKIQSS LDQRRKKAH SEKSDSGDE ALSEEVETF DFNQTLGTS PAKDSSLTI ERLNKQLIL QQQTKEKMD MLQESIRKT LSEFEQEFG EKPPVGQTV SVSEGSSAV EPPTESTVD KNSFEKKK
- >TC014769 RA
atgaaaaaa aactttgct ataggtcct agaagctcg tcagaacct tcccttgcc caaatactg ctccattca gccgagttg gcaaagccc gaaaccttg acatccgtt caatcgaat gacaaccaa aatttgaaa agtcgtgtc agcgacctc gaaaccact ttagcccaa aaagaacaa accgttgaa atcctccgt gaaaaactc gacctcgcc atgaaagac aaacagatc ctgaaggac tccatagtg aagatcacc aaaaattgc gtgtgcgca ctgaagaac aagataatg gacgaagca acggcggat ttaacaacc aaacttgaa gaaaaagaa cgccaaatc cacgacctg caagaagaa cgaaccgtt ctaagcaga gaagtgcaa gactgtgaa aactacatc agcaatcag gtggaccaa atgaagaaa cttgcttcg tacattgac cgcttgacc caaaattcg gaaaaactg caagtggaa cgcaaatcc aaaacatca actgtgtct aatctcaat ggggagaac tgtagctgt gactctaaa cggattaag gagctggaa gcgagaatc aatcgctac aaagcgagg ctgaactcg aaagacgaa agtatcaaa tctctgcag gaacagctc gaatttgag cggatttct cgtgagaag cttgaggaa gctttcctt cggacggca aaaatttac gaggatttg gtccaccag aaccaaggt cttggaaat atgaagtac gaatacgag gcttgtaaa gcacaatgt gagcagttt agagccaca tgtgaagat ctccgtttc aaagcacga aaagtagaa gagttggaa cgagagatt aaccattgc aactgctac atggtgaat caagataac cgaatcacc caacttctg gaagatata gaagtgatg gcgcaacga gaaaacgaa atgaaaatc accatctta aacatggaa accactttg gaggaatac aacgagaaa tttgaaaaa tgccaaaac gagaaagcg tccttgaag gatagaata gaagcgctt gaaacacaa attgaggat tacgacaat atcgtagcc ctgaaggaa gaaaccaat aaaacactt gcggacaat ttagaaaag gaaaaagca gcaaaagcc aaacttgaa tcttcgctt gatacaatt gtcaagacg gacaaacac ttgaaagaa atgttgaca caagccagg caagcaatg aagacaatc acccaaaga ctggaagag tgtgacatc gaacggctg gaaacacaa agagacaac caaatgaaa atatccgag ctggaaaac gagctcatc cttgctaac gccaccatc aaaaaactc aaggaaaat ctcgaccaa atgaaaggt gtgatggac aaaaacgtc gccttgagg acactctca gcccaactg cacgaatct gccaacctc caagtggca caactcaaa tcagcgaac gaagatttg tccagacgt agccaaaaa atggtggaa atgtacgat tatctcaag caggtgttg tacgaaaaa gacaagact tttgaggac ttgaggaac cacttggtg tctttgaga caacgcctg gataacgaa cagaaactg agggaagat acggaaaat attttggct gatgtggca gctaccggg gattcggaa ctaagcgaa cttagaaac gtgataagt gttcttaaa aacgagatt tgttgtttg cactgcgaa ttggaggat ctgatgaat acgacaacg gcaggtcgc ttgaaaaca cgagtgaaa gaacttgaa gtcgaggtt gaagtttta aagtgcgaa agggacaag tatcgtaag tatttggaa aaggttttg cctggttat gttgacccc gaagagttg gaacgtctc cacaacgaa ctgaatcaa atgactgag gaaacagcc cgtttaaat gaagaactc caaaggcag aagaaacta cgaattgat accgtgacg ggattgcaa atgaaaatc gaaaaacag gaaaaggaa aataaaatg attaaagaa caaatcagg gaaagcata gaacagaag aaacgccgc gatccgact ttaccaaac gttgttttc gtcgaaaag aaagacaaa gaggatggc ggtgttgaa tatcgatac ttgtacggt attgccgaa gagaagaaa aagtcaata cagccgccc caacaagac tattacaaa tacgagtgt tatactgtg gaaaatgga atcgagaag agagtgttt ctaggacct ttgcactta acatacatt gatggtgac ggtgaagtg caatatttc tcaattgat cctaaaaca gcaactccc cgaaaggaa ttctacgtg aaagaacgt ggccaaaac aagaaaatt ttatggaat ttgaagacc ggtcccttc aaacttaac atagaagaa gaggtcagt ttgaagcaa aaatacgag gacgctact agagaaata aatcggttg caaagtaga ctcgagttt gtcaaaact gagactagc ggtttgggc gaaaaacta ataaaggac gctcgagac ataaacgat attgattca aagataaca tctgccatt tcggaagct gtaactctg caaaaacag ttggatatg ccaagaagc agaagccag gagtcgttt tcacaagaa cgtgaaact ctcactcga gaaatcgaa aagcaaaag agagagatt gccgatttg ttgcgcaaa attcaagac tcctcttcg gtcggtcat gacgctctg aaggagctt gaaagtaag aaagaggag aatgaaaag ttaaaaaca atggttcgg gatttggag agacagctt cagaatgcc cagtttaag gttgagata ccgaaaagt tcgccacag gttgatact ttagttgag aatctcaaa catcatctc gagaaggaa cagaaacga gtcaatgag ttggaagac ttgttgcac acgtgtcag aagaacgag aaagaagtc aagaaggaa aatgcaaat ctcaagtcg gaaattacg actcttaaa agggacaaa aggatgtcc acagctagt catttgttt gtgaatcag ttgcaacaa gtcgatgat aatttaaag gattggcaa aatcagatt aataataac gtctctcac gttcagaaa caagtcaat gatatcata gataaattg gaaggagcg ccgggaaat accgacgtc gttcaaaaa cttttggag ggtattaaa cacttcgaa gctgatttg gtggacaat gttgttaat aaaaaacga gtggcactg actaatgat ttgcaagag aaacaaatc aatgagttg gaaaaacag ttaagcgac ctacgccaa attatacac aatttggaa gaagagaaa gaaaatgct aaaaagttg gcaccaacg caacaattg gaggagcag ttgagagaa tatcaacgg aaaaatgct gatttaaac agggagctt gaacagctc agaaagagt caaagagac gctcggaga agtatcgat tcagatttt aggcataag agaatttcg gaagtaata agtaaagac agtctgact gataaagac gcacgtgtc aaagaatta gaggacaca ctgcaccag tggcaaaga gattacgat ctagaagtg gggaaactt aaaaaggag attgaaaaa ctgatgatg agaggaaat ttgccacaa ggttgtgaa gcccacgaa acgctaata agagaattg aagaatgat cttgaaaaa tcacaacga aaaaacgaa aattacgaa cggaaaatt gcacacctg gagggcgaa aacgaaaat ctgctcaca atgatggac aaatatgaa ggatcgatt gacagcgct gcggatgat aaaaaagta cgagatctg gtcgaacat ttgcgagag cttcagaga aaaaatgag gctctcaaa acacggaca attcaactc gaactggaa cacggacga atgaaaaac gatctcgat agacttgtc ggtcaggag aataaatca tacgagaag gaaatgcaa cgtttgaga gacttggaa agacagtcg gcaagaagt caggacaaa tatcaaaac ttgaaaaaa agattaatg aaattggag attgagaat aagaatttg aagaaggaa tacatcgat acgttaata gccaaagag aaaatttcg ctcaaaagt cgcgaacaa gaacagagg ttgagagaa acgaacaag ttgttgcgc aaatgccaa gacaagaag caaactttg accatgagg atgctcaag gcacaggtg gatcatgat gatttgaag aaacagcta gagattgat tcacaaaat atcgaaaat gaaaattgt atgaagatt caaaatata aataaagaa ttgattatt aggaacgca caactcgag gaagaaaat aattatttg aagaatctc attccggaa gaagaacgg gcaaaagtg gctcagatt gaatcgcaa atggttgat gtggacgaa gagttgaaa aaattcaat gaacaggca gagttcaag gcgagaatt gcccacttg gagatggaa aatgctcta atgaagaag gaacttgaa gctgttaaa gcgaaagac gtttcgtcg tttgaatat gagacagag aaacgttcg gctgataat ttacaaatt cgagccgta gaactggaa attgaaaat gaatttttg cggagcgaa cttacagac cgagaaaaa atttcatac gataatgga ataaacgaa aggaaaatc aacgaactg gaagaagag atacaaagg ctgaacgaa gccaacgaa aaattacag atgaaaaat gttgacttg gaactcgac catcaatac atgaaggat gaactagat cgtatctca aaagttcgg caaaaactt gaaagcgaa atcccgagc aaagatgta gaaaaacaa aaccacagc ttgaagctc aagaacgac catttggag cttgaaatc aaaaaattg aaggttcag ctcgaaaat atgggcaag gaacagata tctttgcaa tcacacatc caagaaaaa cgtttgaaa gaaatggaa gcgttgatt aacgaatac gaaacaatg agacacaca ccagtggaa cgcactttg aaaagagaa aatcttgac aatgtggag gaagttgag gagaaaatg caagagtgg caagaacaa ttccggcca cagttgtta gacatgaag aaagatctt gagaaactt atgggcaaa agtcgaagt gggaccaat ttaaaacag attgaggaa caattgcgc gaagctaaa accaaagtg gcacatctt gaaacagaa aacaggaat ttgaaagtc gaaaatgaa ataatacgc aaagggatt ccagcaaat gaggctaaa ttggtcaga gacgtggaa cagattttg aaaaatttg ccaaaacct gaattcgca cctaaaagt gtcgaccag aagaagctt aaagacctg gaggcacga ctgagagaa tgtcagcaa gtgaacgac catttacgc aatgaaaaa attgcgctt gataaacac gtccaatct aaacgaata caagacctg gaaacgcaa atacgtgaa caccgattt agagattct tcttcgaaa actgatctt agattacgt gggcaaaaa tcaatcatg cttgacttc caagatatg agagttaaa caattggag gatgaacta ggggcttgt cagaacaga aacgaagca ttgaaagcc gaattggag actttacgt cgtgaaagt gtggcgaca aatagcgac gcaagcacg aaattccaa gagtgtcaa aaacagaat ctactattg aaggacgaa ctggaaagg cttcgtaaa atcaacgat ttgcaacaa aaattagca aaagatttg gacaaacaa ctgcgtgag tgtaagatg aatttggag gagtgtcag agagatgtg aaaagttca aaagaccga aaagtctcg tttcataat attcatgtc aatgttaat gatcttact accgaagaa ttggtcgca gaactggaa cgattgagt aacgaatgc gaacaacaa agacgcatg attcacgag ttggaagaa agtttgagg aaatgtcga gaaaataac agggagttt aagcaagaa aacgacgag ttgaagatt gagcgtcaa tttatgaga caagagtat ggagttcca aaagatgaa aggttgaga caacttcga gacgaacaa aaacgcaaa acaggagga acatctgac gatgtttcg agagcaaca gatgacata agtaccctt caagccaag ttggaactg agtgagaac gataataag aagctcaaa gatgagtta aatactttg acacaagaa cgagacaat cttttaagt gagaaatgc gagttgcaa caaaaaatt caacgtatg agcggtgat tatgaagct gttctcgaa gaaaaggaa aaattgaat ttggtcatt tctgatttg gaaaagaaa attgaagat ttgaccaag ctacagttg gaggttggt aaaggggat actgatgat aagcgctcc cggaaactg gaagaaacc ttgcaaaaa taccacgac aataacgaa aatcttaaa atacgaatt gtgcatctc gaggaggag aaaaccaat ttgaagaag gaactgagt gctcgtatc gaaaaagaa aagaaaacg cctgggagt gaacaacaa gctcgacaa atcagaaaa ttggaacaa accttgcaa aaataccac gataataac gagaatttg aagcgtaaa attgcgttt ttggaggcc gattaccag gaaaagcga gtggtggaa ctggaggaa caagttcaa aagtgtcag ataaataac gatgggttg aaacaagta acagtgcaa ctggaatct gagataaag tatttaaaa gaagagctt ggaacattt cttcaaaaa gaaaagaaa gtgacccgg aaacggcaa actacagcg tccgaaaaa aacaagccc aaacaatcg ctttttatg cagccccca gtctatgcc atgcctgtt tttatacaa ccacctttt gtgtcctac gggaattat ttagcgcca caagttgca agtactgga actggtggt gatattgaa caattgtcc cacttggtg gataagatt cagagtagt ctcgatcag cgtagaaaa aaggcacac agtgaaaaa agtgatagt ggggatgag gcactttca gaagaagtt gaaacgttc gatttcaac caaacattg ggcacaagt ccagcaaag gactcaagt ctcacaatt gagagattg aacaaacaa ctgattttg caacaacaa accaaagaa aaaatggat atgttacaa gagagcata aggaaaacg ttgagcgag tttgagcaa gaattcggc gaaaagcca ccagtcgga caaacagta agtgttagt gaaggttcg agtgctgtg gaacccccc acagagtcg actgtggac aaaaattcg tttgagaag aaaaaatag
- >TC014769 RA
taaaaacta tttattagg tattgaaaa tgaaaaaaa actttgcta taggtccta gaagctcgt cagaacctt cccttgccc aaatactgc tccattcag ccgagttgg caaagcccg aaaccttga catccgttc aatcgaatg acaaccaaa atttgaaaa gtcgtgtca gcgacctcg aaaccactt tagcccaaa aagaacaaa ccgttgaaa tcctccgtg aaaaactcg acctcgcca tgaaagaca aacagatcc tgaaggact ccatagtga agatcacca aaaattgcg tgtgcgcac tgaagaaca agataatgg acgaagcaa cggcggatt taacaacca aacttgaag aaaaagaac gccaaatcc acgacctgc aagaagaac gaaccgttc taagcagag aagtgcaag actgtgaaa actacatca gcaatcagg tggaccaaa tgaagaaac ttgcttcgt acattgacc gcttgaccc aaaattcgg aaaaactgc aagtggaac gcaaatcca aaacatcaa ctgtgtcta atctcaatg gggagaact gtagctgtg actctaaac ggattaagg agctggaag cgagaatca atcgctaca aagcgaggc tgaactcga aagacgaaa gtatcaaat ctctgcagg aacagctcg aatttgagc ggatttctc gtgagaagc ttgaggaag ctttccttc ggacggcaa aaatttacg aggatttgg tccaccaga accaaggtc ttggaaata tgaagtacg aatacgagg cttgtaaag cacaatgtg agcagttta gagccacat gtgaagatc tccgtttca aagcacgaa aagtagaag agttggaac gagagatta accattgca actgctaca tggtgaatc aagataacc gaatcaccc aacttctgg aagatatag aagtgatgg cgcaacgag aaaacgaaa tgaaaatca ccatcttaa acatggaaa ccactttgg aggaataca acgagaaat ttgaaaaat gccaaaacg agaaagcgt ccttgaagg atagaatag aagcgcttg aaacacaaa ttgaggatt acgacaata tcgtagccc tgaaggaag aaaccaata aaacacttg cggacaatt tagaaaagg aaaaagcag caaaagcca aacttgaat cttcgcttg atacaattg tcaagacgg acaaacact tgaaagaaa tgttgacac aagccaggc aagcaatga agacaatca cccaaagac tggaagagt gtgacatcg aacggctgg aaacacaaa gagacaacc aaatgaaaa tatccgagc tggaaaacg agctcatcc ttgctaacg ccaccatca aaaaactca aggaaaatc tcgaccaaa tgaaaggtg tgatggaca aaaacgtcg ccttgagga cactctcag cccaactgc acgaatctg ccaacctcc aagtggcac aactcaaat cagcgaacg aagatttgt ccagacgta gccaaaaaa tggtggaaa tgtacgatt atctcaagc aggtgttgt acgaaaaag acaagactt ttgaggact tgaggaacc acttggtgt ctttgagac aacgcctgg ataacgaac agaaactga gggaagata cggaaaata ttttggctg atgtggcag ctaccgggg attcggaac taagcgaac ttagaaacg tgataagtg ttcttaaaa acgagattt gttgtttgc actgcgaat tggaggatc tgatgaata cgacaacgg caggtcgct tgaaaacac gagtgaaag aacttgaag tcgaggttg aagttttaa agtgcgaaa gggacaagt atcgtaagt atttggaaa aggttttgc ctggttatg ttgaccccg aagagttgg aacgtctcc acaacgaac tgaatcaaa tgactgagg aaacagccc gtttaaatg aagaactcc aaaggcaga agaaactac gaattgata ccgtgacgg gattgcaaa tgaaaatcg aaaaacagg aaaaggaaa ataaaatga ttaaagaac aaatcaggg aaagcatag aacagaaga aacgccgcg atccgactt taccaaacg ttgttttcg tcgaaaaga aagacaaag aggatggcg gtgttgaat atcgatact tgtacggta ttgccgaag agaagaaaa agtcaatac agccgcccc aacaagact attacaaat acgagtgtt atactgtgg aaaatggaa tcgagaaga gagtgtttc taggacctt tgcacttaa catacattg atggtgacg gtgaagtgc aatatttct caattgatc ctaaaacag caactcccc gaaaggaat tctacgtga aagaacgtg gccaaaaca agaaaattt tatggaatt tgaagaccg gtcccttca aacttaaca tagaagaag aggtcagtt tgaagcaaa aatacgagg acgctacta gagaaataa atcggttgc aaagtagac tcgagtttg tcaaaactg agactagcg gtttgggcg aaaaactaa taaaggacg ctcgagaca taaacgata ttgattcaa agataacat ctgccattt cggaagctg taactctgc aaaaacagt tggatatgc caagaagca gaagccagg agtcgtttt cacaagaac gtgaaactc tcactcgag aaatcgaaa agcaaaaga gagagattg ccgatttgt tgcgcaaaa ttcaagact cctcttcgg tcggtcatg acgctctga aggagcttg aaagtaaga aagaggaga atgaaaagt taaaaacaa tggttcggg atttggaga gacagcttc agaatgccc agtttaagg ttgagatac cgaaaagtt cgccacagg ttgatactt tagttgaga atctcaaac atcatctcg agaaggaac agaaacgag tcaatgagt tggaagact tgttgcaca cgtgtcaga agaacgaga aagaagtca agaaggaaa atgcaaatc tcaagtcgg aaattacga ctcttaaaa gggacaaaa ggatgtcca cagctagtc atttgtttg tgaatcagt tgcaacaag tcgatgata atttaaagg attggcaaa atcagatta ataataacg tctctcacg ttcagaaac aagtcaatg atatcatag ataaattgg aaggagcgc cgggaaata ccgacgtcg ttcaaaaac ttttggagg gtattaaac acttcgaag ctgatttgg tggacaatg ttgttaata aaaaacgag tggcactga ctaatgatt tgcaagaga aacaaatca atgagttgg aaaaacagt taagcgacc tacgccaaa ttatacaca atttggaag aagagaaag aaaatgcta aaaagttgg caccaacgc aacaattgg aggagcagt tgagagaat atcaacgga aaaatgctg atttaaaca gggagcttg aacagctca gaaagagtc aaagagacg ctcggagaa gtatcgatt cagatttta ggcataaga gaatttcgg aagtaataa gtaaagaca gtctgactg ataaagacg cacgtgtca aagaattag aggacacac tgcaccagt ggcaaagag attacgatc tagaagtgg ggaaactta aaaaggaga ttgaaaaac tgatgatga gaggaaatt tgccacaag gttgtgaag cccacgaaa cgctaataa gagaattga agaatgatc ttgaaaaat cacaacgaa aaaacgaaa attacgaac ggaaaattg cacacctgg agggcgaaa acgaaaatc tgctcacaa tgatggaca aatatgaag gatcgattg acagcgctg cggatgata aaaaagtac gagatctgg tcgaacatt tgcgagagc ttcagagaa aaaatgagg ctctcaaaa cacggacaa ttcaactcg aactggaac acggacgaa tgaaaaacg atctcgata gacttgtcg gtcaggaga ataaatcat acgagaagg aaatgcaac gtttgagag acttggaaa gacagtcgg caagaagtc aggacaaat atcaaaact tgaaaaaaa gattaatga aattggaga ttgagaata agaatttga agaaggaat acatcgata cgttaatag ccaaagaga aaatttcgc tcaaaagtc gcgaacaag aacagaggt tgagagaaa cgaacaagt tgttgcgca aatgccaag acaagaagc aaactttga ccatgagga tgctcaagg cacaggtgg atcatgatg atttgaaga aacagctag agattgatt cacaaaata tcgaaaatg aaaattgta tgaagattc aaaatataa ataaagaat tgattatta ggaacgcac aactcgagg aagaaaata attatttga agaatctca ttccggaag aagaacggg caaaagtgg ctcagattg aatcgcaaa tggttgatg tggacgaag agttgaaaa aattcaatg aacaggcag agttcaagg cgagaattg cccacttgg agatggaaa atgctctaa tgaagaagg aacttgaag ctgttaaag cgaaagacg tttcgtcgt ttgaatatg agacagaga aacgttcgg ctgataatt tacaaattc gagccgtag aactggaaa ttgaaaatg aatttttgc ggagcgaac ttacagacc gagaaaaaa tttcatacg ataatggaa taaacgaaa ggaaaatca acgaactgg aagaagaga tacaaaggc tgaacgaag ccaacgaaa aattacaga tgaaaaatg ttgacttgg aactcgacc atcaataca tgaaggatg aactagatc gtatctcaa aagttcggc aaaaacttg aaagcgaaa tcccgagca aagatgtag aaaaacaaa accacagct tgaagctca agaacgacc atttggagc ttgaaatca aaaaattga aggttcagc tcgaaaata tgggcaagg aacagatat ctttgcaat cacacatcc aagaaaaac gtttgaaag aaatggaag cgttgatta acgaatacg aaacaatga gacacacac cagtggaac gcactttga aaagagaaa atcttgaca atgtggagg aagttgagg agaaaatgc aagagtggc aagaacaat tccggccac agttgttag acatgaaga aagatcttg agaaactta tgggcaaaa gtcgaagtg ggaccaatt taaaacaga ttgaggaac aattgcgcg aagctaaaa ccaaagtgg cacatcttg aaacagaaa acaggaatt tgaaagtcg aaaatgaaa taatacgca aagggattc cagcaaatg aggctaaat tggtcagag acgtggaac agattttga aaaatttgc caaaacctg aattcgcac ctaaaagtg tcgaccaga agaagctta aagacctgg aggcacgac tgagagaat gtcagcaag tgaacgacc atttacgca atgaaaaaa ttgcgcttg ataaacacg tccaatcta aacgaatac aagacctgg aaacgcaaa tacgtgaac accgattta gagattctt cttcgaaaa ctgatctta gattacgtg ggcaaaaat caatcatgc ttgacttcc aagatatga gagttaaac aattggagg atgaactag gggcttgtc agaacagaa acgaagcat tgaaagccg aattggaga ctttacgtc gtgaaagtg tggcgacaa atagcgacg caagcacga aattccaag agtgtcaaa aacagaatc tactattga aggacgaac tggaaaggc ttcgtaaaa tcaacgatt tgcaacaaa aattagcaa aagatttgg acaaacaac tgcgtgagt gtaagatga atttggagg agtgtcaga gagatgtga aaagttcaa aagaccgaa aagtctcgt ttcataata ttcatgtca atgttaatg atcttacta ccgaagaat tggtcgcag aactggaac gattgagta acgaatgcg aacaacaaa gacgcatga ttcacgagt tggaagaaa gtttgagga aatgtcgag aaaataaca gggagttta agcaagaaa acgacgagt tgaagattg agcgtcaat ttatgagac aagagtatg gagttccaa aagatgaaa ggttgagac aacttcgag acgaacaaa aacgcaaaa caggaggaa catctgacg atgtttcga gagcaacag atgacataa gtacccttc aagccaagt tggaactga gtgagaacg ataataaga agctcaaag atgagttaa atactttga cacaagaac gagacaatc ttttaagtg agaaatgcg agttgcaac aaaaaattc aacgtatga gcggtgatt atgaagctg ttctcgaag aaaaggaaa aattgaatt tggtcattt ctgatttgg aaaagaaaa ttgaagatt tgaccaagc tacagttgg aggttggta aaggggata ctgatgata agcgctccc ggaaactgg aagaaacct tgcaaaaat accacgaca ataacgaaa atcttaaaa tacgaattg tgcatctcg aggaggaga aaaccaatt tgaagaagg aactgagtg ctcgtatcg aaaaagaaa agaaaacgc ctgggagtg aacaacaag ctcgacaaa tcagaaaat tggaacaaa ccttgcaaa aataccacg ataataacg agaatttga agcgtaaaa ttgcgtttt tggaggccg attaccagg aaaagcgag tggtggaac tggaggaac aagttcaaa agtgtcaga taaataacg atgggttga aacaagtaa cagtgcaac tggaatctg agataaagt atttaaaag aagagcttg gaacatttc ttcaaaaag aaaagaaag tgacccgga aacggcaaa ctacagcgt ccgaaaaaa acaagccca aacaatcgc tttttatgc agcccccag tctatgcca tgcctgttt ttatacaac caccttttg tgtcctacg ggaattatt tagcgccac aagttgcaa gtactggaa ctggtggtg atattgaac aattgtccc acttggtgg ataagattc agagtagtc tcgatcagc gtagaaaaa aggcacaca gtgaaaaaa gtgatagtg gggatgagg cactttcag aagaagttg aaacgttcg atttcaacc aaacattgg gcacaagtc cagcaaagg actcaagtc tcacaattg agagattga acaaacaac tgattttgc aacaacaaa ccaaagaaa aaatggata tgttacaag agagcataa ggaaaacgt tgagcgagt ttgagcaag aattcggcg aaaagccac cagtcggac aaacagtaa gtgttagtg aaggttcga gtgctgtgg aacccccca cagagtcga ctgtggaca aaaattcgt ttgagaaga aaaaatagt cggttttgt gccgttttt gtttgtgat ctgtcttgt gccgtcttg tgaaataaa gtataattt tttac
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