Status
Please wait ...
Gene information
General information
TC014741 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14314239..14329707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014741
    iBeetle screen iB_05685

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014741 RA
      MREDMMDEV AVTSASEFD LANIHEEEF ELHTTYIVP 
      DLPVEPGTP NRAEATLPR NLVLKTSQA MSDAQGVWS 
      TGYIPRGTR FGPLVGEIY AKDAVPSSA NRKYFWRVQ 
      MREGFYRNS SNDSKIYKD NELYYYIDG FDTSKANWM 
      RYVNPAYSS ESQNLIACQ YKMNIYFYT IKPILPNQE 
      LLVWYCKEF AERLNYPLT GEQMLQRIR QQVQQSSEV 
      ITVSTTEPP AKDGPYEHQ LTPTDGSVR SDEGYHSNG 
      YHDDAFTPP EDSSDSDSE NNYVLDFSK KPNSKETAV 
      IKPVSPETQ KNEYRKVKI KISKAYHYK SKGVESDHE 
      MDKESHTDA AVPEVTTLT PRIITPPST VIVIDSPPP 
      EVPNNKPFY EPEVKPQNV ISRYTPPTS SILENILLR 
      NRIDTNNND NNQRQPNAT PPPSSPTEM AYSYKKSHR 
      YGTVPCSPD SSSNVQVQN MLPPSPAML KNHPPSPVQ 
      PLYVPHDNY PNHMQPSNY YNIYPSNGI PHSTITSTP 
      TTTYSPPAT HHHNGSNLM IPASHINLT HHLLTPLPP 
      LQTSGRSSP CSRSPEGGS PGSPNTNQN RGYRSLPYP 
      LKKKDGKMH YECNVCCKT FGQLSNLKV HLRTHSGER 
      PFKCNVCTK SFTQLAHLQ KHHLVHTGE RPHECGICK 
      KRFSSTSNL KTHLRLHSG QKPYACDFC PAKFTQFVH 
      LKLHKRLHT NERPYICQE CGKNYISAS GLRTHWKTT 
      SCRPNNIDE ELNGEGSPN GYYEYAGSD GSLGSIDMN 
      KDMHEDSKE FLEVQQAPI GHAVLHPGL SRPLPPQQR 
      QPPQPPIHP GKETRPSVI ESSQPHIIE CT
    CDS sequence
    • >TC014741 RA
      atgagagag gacatgatg gatgaggta gctgtaacg 
      tccgccagc gaattcgac ttggccaat atccatgag 
      gaagagttc gaattgcat accacctac atcgttccg 
      gatcttccg gttgagccc ggaacgccg aaccgggcg 
      gaagctacc cttccccgc aacctagtg ctgaagaca 
      tcgcaggca atgtctgat gctcagggc gtttggagt 
      acggggtac atcccacga gggacgcgc tttgggcct 
      ctcgtggga gaaatctat gccaaagat gcggtacca 
      agctcggcg aaccggaag tacttttgg agggttcag 
      atgagagag ggcttttat agaaacagt tctaacgac 
      agcaagata tacaaagat aacgaatta tattattat 
      attgatgga tttgataca tctaaagct aactggatg 
      cgatacgtg aatcctgct tattccagc gaatcgcaa 
      aatttaatc gcctgtcaa tacaaaatg aatatctat 
      ttttacact atcaaacca attcttccc aatcaggaa 
      cttcttgtc tggtactgc aaagaattc gccgaaaga 
      cttaattat cccttgact ggtgaacaa atgctacaa 
      agaattaga caacaagta cagcaatct tctgaagtg 
      ataactgtt agtacgact gaacctcct gcaaaagac 
      ggtccctac gaacaccaa ctaacacca actgatggt 
      agtgtgaga tcagacgaa ggatatcat agtaatggc 
      tatcacgac gatgctttc acaccacct gaagattcg 
      agtgattca gatagtgag aataattac gttcttgat 
      tttagtaaa aaaccaaat tcgaaagaa accgcggta 
      ataaaaccg gtttctccc gaaacccaa aagaacgaa 
      tacaggaaa gtgaaaata aaaatctcg aaagcttac 
      cactacaag tcgaaagga gtggaaagc gaccacgaa 
      atggacaag gaatcccac actgacgct gcagtacct 
      gaagtaaca acactaacc ccgagaata atcacgccc 
      ccaagtaca gtaattgtg attgattct cctccacct 
      gaagttcct aacaataaa cctttctac gaacctgaa 
      gtcaagccc cagaatgtt atttcgcgg tatacgccg 
      cccacttct agcatcctt gagaatatt ttgctaagg 
      aatcgaatt gataccaat aataacgac aacaatcag 
      agacaacca aacgcaacc cctcctcca agctccccc 
      accgaaatg gcttattca tacaagaaa agtcaccgg 
      tatggcact gtcccctgc agtccagac tcaagttca 
      aatgtccaa gttcaaaac atgctccct cctagtccc 
      gcaatgctt aagaaccac cccccaagt cccgtccag 
      cccttgtac gtcccccac gacaactac cctaaccac 
      atgcaaccc tccaactac tacaacatt tacccttca 
      aacggcatc ccccatagt actatcacc tccaccccc 
      acaaccacc tactcgccc ccagccacc caccaccac 
      aacggttcg aatctaatg atcccagcg tcccacatc 
      aacctcacc caccacctc ctcacaccc ctaccccca 
      ctgcaaacc agcggcagg tcctccccc tgctcgcgg 
      agccccgag ggcgggtcc cccggtagc cccaacacc 
      aaccagaac cgcggctat cgcagcctg ccctacccc 
      ctgaagaag aaggacggg aaaatgcac tacgagtgc 
      aacgtttgt tgcaaaacc ttcggccag ttgtccaac 
      ttgaaggtc catttaagg acacacagt ggcgaacgc 
      cccttcaag tgcaacgtc tgcaccaag tccttcacg 
      cagttggcc cacctccag aagcaccac ctggtgcac 
      actggagaa aggccacac gagtgtggc atttgtaag 
      aagcgattc tcctccacg tccaacctt aaaacccac 
      ttgaggcta catagtggc cagaaaccc tacgcgtgc 
      gatttctgc cccgccaag ttcacgcag ttcgtccac 
      ttgaagctc cacaagagg ctgcatacg aacgaacgg 
      ccgtatatt tgtcaggag tgcgggaaa aactacatc 
      agtgcgtct ggcttacgg acgcattgg aagacgacg 
      agttgtagg ccgaacaat attgatgag gagttgaac 
      ggcgaaggc tcgcctaat gggtactac gagtacgct 
      gggtcggat ggaagtcta ggatcgata gatatgaac 
      aaggacatg catgaggat tcgaaggag tttctggag 
      gtgcagcag gcgccgata gggcacgcg gtgctgcac 
      cccgggctg tcgaggcct ctgccgccg cagcagcgg 
      cagcccccg cagcccccc atccacccc gggaaggag 
      acgcggccg agcgtgatc gagtcgtcc caaccccac 
      ataattgaa tgtacctaa
    mRNA sequence
    • >TC014741 RA
      caaaaggac tgtcacagt tttcaaata taatacaaa 
      ttaccggtt gacctagag aaaatttgt ttagacttg 
      cagttacgg tggtgaaat ttgttaaga agtcgtaaa 
      ttaaaaccg cagggactc acttagagt cgtttggcc 
      gtaacagaa gccatttct taatactgc cacaggggg 
      caggggttt aagcaaatt tttcgcatt tgttgtaat 
      taggtgggc tggccggcc tattttcag tagagagtg 
      agccggtgg tggagttgg tgcccgttt gtgcgctat 
      taactgaaa ttcttcgga gacttcaat agtcgatca 
      ggcgcgaaa acttgaaat tcgatggcg ctttctttc 
      gttttgtaa taattttgc gtggaaatt gagtcatat 
      atcacctgt cgaagttct gtagaatcc tctggtaaa 
      gccgcaaag agaaaggca tttcactga atacctaaa 
      ttcgtcagt tgaacttgc gtgaaataa ggttatatc 
      atttaccat aaacaaaat gacgcggat gagtcaaag 
      acctcctct gcataagtg gaactttta ctcgattta 
      atagtcatc ccgttctga ttccggaga attcgagcg 
      aaagaaagg tgatggccc tccggagcc ggctccggg 
      tcgggtttg gtgagtgag cgtctgatg agagcgctt 
      ggtccatga accctaaaa taacgacaa aaagaactt 
      cctcaaacg tgagctgca gcagtgaag ttgctggca 
      acgtcttga ggccgctca gaatttgga ggttgttga 
      ccggagttc ccgttagtt agagcttcc aactatcac 
      cacttggcg ctgtgtgaa aaccatcac ggtcaagtt 
      taactcgga tcggtgttt agtttctaa aagactgcg 
      aaggttgaa gaacgtaat ggtgtgttt gacattgcc 
      ctagacatg cggcagtga cgactcttc agccacccc 
      cggagtccg gattaagtg cttcgagag gagacgcgt 
      caaaatttt cgttggaca tggtgatac gaaattccg 
      ttcggacca cttttttcg ataaagact gatccaagt 
      tttgaactt gaaaaaaaa gtgtgtgtg tgtgacttt 
      tcgtggata tagggttta gtgacgctg ccaaaattt 
      tctggatac cttggatat tatatattc ctccgaaag 
      actgccacg aaggactta aatgccttg aagaacagc 
      tgcagtcct caaaaaagg ccaaaatga gagaggaca 
      tgatggatg aggtagctg taacgtccg ccagcgaat 
      tcgacttgg ccaatatcc atgaggaag agttcgaat 
      tgcatacca cctacatcg ttccggatc ttccggttg 
      agcccggaa cgccgaacc gggcggaag ctacccttc 
      cccgcaacc tagtgctga agacatcgc aggcaatgt 
      ctgatgctc agggcgttt ggagtacgg ggtacatcc 
      cacgaggga cgcgctttg ggcctctcg tgggagaaa 
      tctatgcca aagatgcgg taccaagct cggcgaacc 
      ggaagtact tttggaggg ttcagatga gagagggct 
      tttatagaa acagttcta acgacagca agatataca 
      aagataacg aattatatt attatattg atggatttg 
      atacatcta aagctaact ggatgcgat acgtgaatc 
      ctgcttatt ccagcgaat cgcaaaatt taatcgcct 
      gtcaataca aaatgaata tctattttt acactatca 
      aaccaattc ttcccaatc aggaacttc ttgtctggt 
      actgcaaag aattcgccg aaagactta attatccct 
      tgactggtg aacaaatgc tacaaagaa ttagacaac 
      aagtacagc aatcttctg aagtgataa ctgttagta 
      cgactgaac ctcctgcaa aagacggtc cctacgaac 
      accaactaa caccaactg atggtagtg tgagatcag 
      acgaaggat atcatagta atggctatc acgacgatg 
      ctttcacac cacctgaag attcgagtg attcagata 
      gtgagaata attacgttc ttgatttta gtaaaaaac 
      caaattcga aagaaaccg cggtaataa aaccggttt 
      ctcccgaaa cccaaaaga acgaataca ggaaagtga 
      aaataaaaa tctcgaaag cttaccact acaagtcga 
      aaggagtgg aaagcgacc acgaaatgg acaaggaat 
      cccacactg acgctgcag tacctgaag taacaacac 
      taaccccga gaataatca cgcccccaa gtacagtaa 
      ttgtgattg attctcctc cacctgaag ttcctaaca 
      ataaacctt tctacgaac ctgaagtca agccccaga 
      atgttattt cgcggtata cgccgccca cttctagca 
      tccttgaga atattttgc taaggaatc gaattgata 
      ccaataata acgacaaca atcagagac aaccaaacg 
      caacccctc ctccaagct cccccaccg aaatggctt 
      attcataca agaaaagtc accggtatg gcactgtcc 
      cctgcagtc cagactcaa gttcaaatg tccaagttc 
      aaaacatgc tccctccta gtcccgcaa tgcttaaga 
      accaccccc caagtcccg tccagccct tgtacgtcc 
      cccacgaca actacccta accacatgc aaccctcca 
      actactaca acatttacc cttcaaacg gcatccccc 
      atagtacta tcacctcca cccccacaa ccacctact 
      cgcccccag ccacccacc accacaacg gttcgaatc 
      taatgatcc cagcgtccc acatcaacc tcacccacc 
      acctcctca cacccctac ccccactgc aaaccagcg 
      gcaggtcct ccccctgct cgcggagcc ccgagggcg 
      ggtcccccg gtagcccca acaccaacc agaaccgcg 
      gctatcgca gcctgccct accccctga agaagaagg 
      acgggaaaa tgcactacg agtgcaacg tttgttgca 
      aaaccttcg gccagttgt ccaacttga aggtccatt 
      taaggacac acagtggcg aacgcccct tcaagtgca 
      acgtctgca ccaagtcct tcacgcagt tggcccacc 
      tccagaagc accacctgg tgcacactg gagaaaggc 
      cacacgagt gtggcattt gtaagaagc gattctcct 
      ccacgtcca accttaaaa cccacttga ggctacata 
      gtggccaga aaccctacg cgtgcgatt tctgccccg 
      ccaagttca cgcagttcg tccacttga agctccaca 
      agaggctgc atacgaacg aacggccgt atatttgtc 
      aggagtgcg ggaaaaact acatcagtg cgtctggct 
      tacggacgc attggaaga cgacgagtt gtaggccga 
      acaatattg atgaggagt tgaacggcg aaggctcgc 
      ctaatgggt actacgagt acgctgggt cggatggaa 
      gtctaggat cgatagata tgaacaagg acatgcatg 
      aggattcga aggagtttc tggaggtgc agcaggcgc 
      cgatagggc acgcggtgc tgcaccccg ggctgtcga 
      ggcctctgc cgccgcagc agcggcagc ccccgcagc 
      cccccatcc accccggga aggagacgc ggccgagcg 
      tgatcgagt cgtcccaac cccacataa ttgaatgta 
      cctaagatg tattcctgc tcatgtagt gtagttttg 
      ttaagtttt ttaaggttt atttattac taggctagt 
      actgaggta ataaagtgt gttcacgaa ggactggac 
      acggcgtcg aataaataa gaagtgtaa ttttacttt 
      tctaaagat actcattgc gattttgtt gagataatt 
      tattgatct atttattga tagttgttt aatgaaagt 
      attttatgg aattaaccg aattttgac tttagactg 
      atcacgtat aaagcaata ctatttatg acttgcacg 
      aaattgtct agacttgtg tatttattt gtatttgaa 
      tgttttagg agaacgtag tcgttttta cgttgtaga 
      ttttgtatt gaatgtgtt tgcagttta tatacatta 
      gcgtattcg acttggtac ttgattgga tactcgtct 
      ttaactcca tattgagaa actacatcc cacgtttca 
      gttgacaaa ttcatgacg gttttttcg attttctgc 
      aagcgtagt tgcttgata tgctgttaa atccataac 
      ttacttatg ttagaatat atccaaata agtataaac 
      caaataaga tcatcctct tcatgtatt actactatt 
      tatacatgt aaagttcaa tgtatgtaa ttgcgtaaa 
      tctaaatgg tgcttgtga cttgtttaa tttaagtgt 
      gtatgaaat tcctgaagc ttagcagtg gaaattatt 
      tgtctgctt gcactaaaa caaaaaatc agtattaag 
      ttgattcga atcgtggat actgtacat catcgatca 
      ttgtttttg taaaaacat atttttgta tatttcgtc 
      tctcacatg tatataatt tcaatttta ggtacctac 
      ttgaaatta atgtaaatt tttgtaaat tcgattgtg 
      aatattttt aaaattagc gacaaactt gccttgtac 
      ataaaaatt atttattca tttttcaca aattgcaaa 
      accttcatt tacgttttt tccgacagt tttgttttt 
      taagttatt tgagataac acacaatag tataatacg 
      attattatt aatgtagta tgtactgtg tacaataag 
      atttgtttt ttattgtta tttagttgt actttgtaa 
      gttatttat ttcctgttt ttcatatca cttgtttgc 
      aaaatttcg aactgaata aatagcggt gtttgcatc 
      attgtcgag gaaaattag tttaggtgg gtaccttcg 
      tttttgtta ttacaaagg tatgatttt ataaacgtc 
      tcatcattt caaccctct ctcatttct cccacatta 
      tttaagtcg taacgtgtt agtcttaga aatgttcgc 
      tatttattt gtcgtagtt gtttaatta ttcatttgg 
      aactgttac attcaaaat attttggta tatctcgtg 
      catgtaaaa atgttcata tgagtatta aaacataaa 
      acttgacgg cggctttta ttagtcaac acttgttcc 
      ataaaaaca tttttaaaa acactttga aaagcaaaa 
      ttttattaa taaatttta tgattacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY