Status
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Gene information
General information
TC014731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14225069..14246394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05684

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014731 RA
      MNEFDGVDN FTEDTMSTA SIYLEASND SDQHNGTDF 
      AMEYEEYIY DKVYIRIIF ISLYTVVFC LCFFGNLLV 
      ILVVTLSRR LRSITNFFL ANLAVADFC VAVFCVYQN 
      LFIYFVDSW MLGEFLCKM YMFIQSLSN TASIFILVI 
      ICIERYFAI IYPITCKQI LTPRRLRLI ILGVWITCI 
      LYSAPKFYW GNTVTVPTK NMTETMCVL NRTKYNSEL 
      FDVTHFVLF YNIPLIIMS LLYSRIAIC LWNSSQQLK 
      KQLNASTNP NQYQGLCQR QSSRYRSDK KVANGDAKL 
      LQNSSSNMS TSNSSQNVL AARRGVIKM LIIVVCAFA 
      VCNLPFHAR KMWQYRSSN YHGDTNFSA LFTPLTFLA 
      TYFNSGVNP LLYAFLSKN FRRGMREIL LCSCRRRRQ 
      PGKSNHLSL HVGIQRHAS TRSTVKANA TTVTIVNND 
      HSSECSHDV
    CDS sequence
    • >TC014731 RA
      atgaatgag tttgacggc gttgataat ttcactgaa 
      gacacaatg tcaacagcc agcatctat ctggaggca 
      tctaacgac tccgaccag cacaatggc accgacttt 
      gccatggaa tacgaggag tacatctat gataaagtc 
      tacattagg attatattc atctcgctc tacaccgtc 
      gtcttctgt ctgtgcttt ttcgggaat ttgctggtt 
      attttggtc gtgacgtta agtcgacgc cttcgatca 
      atcaccaac ttcttccta gctaatctg gcggtggcc 
      gacttctgc gtggctgtt ttttgcgtc taccaaaat 
      ctgtttata tacttcgtc gacagctgg atgcttggc 
      gagttcctg tgcaagatg tacatgttc atccagtca 
      ctcagtaac acagcaagc atttttatc ctcgttatc 
      atctgcatc gagaggtat tttgccata atttacccc 
      attacctgc aaacagatc ctcacgccg aggcgactt 
      aggttaatc attttagga gtctggata acgtgcatc 
      ctctacagc gccccgaaa ttttactgg ggcaacact 
      gtgaccgtc ccaaccaag aacatgaca gagactatg 
      tgcgtcctg aacaggacc aagtacaat tcggaattg 
      ttcgacgtg actcacttc gttttgttt tacaacatc 
      ccgctaatt attatgtca ttgctctat tcgaggatt 
      gcgatttgt ctctggaat agctcgcaa cagttgaaa 
      aaacaactc aacgcttcc acgaatccc aatcagtac 
      caggggctg tgtcaacgg caatccagt cggtatcgg 
      agcgataaa aaggtggcc aacggcgac gcgaaactc 
      ctgcagaac tcctcctca aacatgtcc acatccaac 
      tccagccag aacgtcctt gcagctcgc agaggcgtc 
      atcaagatg ctcataatc gtggtgtgt gctttcgcc 
      gtgtgcaac ttgcccttc catgctcga aaaatgtgg 
      caatatcgg tcatccaat tatcacggg gacaccaac 
      tttagcgcc ctcttcacc ccattgact ttcctcgcc 
      acatatttc aactcggga gtcaaccct ttgctgtac 
      gccttcctg tcgaaaaac ttcaggcgg ggcatgagg 
      gagatcctc ctgtgctcc tgccgcagg aggcgccag 
      ccggggaag agcaaccat ctgagcctg catgtgggc 
      atccagagg cacgcgtcc accaggtcc accgtcaaa 
      gccaacgcc acaacggtc acgattgtg aacaatgac 
      cacagctcg gaatgctcg cacgatgta tga
    mRNA sequence
    • >TC014731 RA
      tgctggcta aagacctcc tgtgcatca actcgtgtt 
      cgttgtgcg ttcggtaat ttaaaaaaa cgctagtat 
      tcagtcggc gctcacgca caatttcaa gtggtttcg 
      tgttaaaag ctctcggag tattaaaaa atgaagtac 
      aaatcgaag tagctaaat caacgaaat gaagcgctt 
      gcatccttc gtctgggag ataaactcg aaagccggc 
      tttttactt ccatccgga cgatcatga atgagtttg 
      acggcgttg ataatttca ctgaagaca caatgtcaa 
      cagccagca tctatctgg aggcatcta acgactccg 
      accagcaca atggcaccg actttgcca tggaatacg 
      aggagtaca tctatgata aagtctaca ttaggatta 
      tattcatct cgctctaca ccgtcgtct tctgtctgt 
      gctttttcg ggaatttgc tggttattt tggtcgtga 
      cgttaagtc gacgccttc gatcaatca ccaacttct 
      tcctagcta atctggcgg tggccgact tctgcgtgg 
      ctgtttttt gcgtctacc aaaatctgt ttatatact 
      tcgtcgaca gctggatgc ttggcgagt tcctgtgca 
      agatgtaca tgttcatcc agtcactca gtaacacag 
      caagcattt ttatcctcg ttatcatct gcatcgaga 
      ggtattttg ccataattt accccatta cctgcaaac 
      agatcctca cgccgaggc gacttaggt taatcattt 
      taggagtct ggataacgt gcatcctct acagcgccc 
      cgaaatttt actggggca acactgtga ccgtcccaa 
      ccaagaaca tgacagaga ctatgtgcg tcctgaaca 
      ggaccaagt acaattcgg aattgttcg acgtgactc 
      acttcgttt tgttttaca acatcccgc taattatta 
      tgtcattgc tctattcga ggattgcga tttgtctct 
      ggaatagct cgcaacagt tgaaaaaac aactcaacg 
      cttccacga atcccaatc agtaccagg ggctgtgtc 
      aacggcaat ccagtcggt atcggagcg ataaaaagg 
      tggccaacg gcgacgcga aactcctgc agaactcct 
      cctcaaaca tgtccacat ccaactcca gccagaacg 
      tccttgcag ctcgcagag gcgtcatca agatgctca 
      taatcgtgg tgtgtgctt tcgccgtgt gcaacttgc 
      ccttccatg ctcgaaaaa tgtggcaat atcggtcat 
      ccaattatc acggggaca ccaacttta gcgccctct 
      tcaccccat tgactttcc tcgccacat atttcaact 
      cgggagtca accctttgc tgtacgcct tcctgtcga 
      aaaacttca ggcggggca tgagggaga tcctcctgt 
      gctcctgcc gcaggaggc gccagccgg ggaagagca 
      accatctga gcctgcatg tgggcatcc agaggcacg 
      cgtccacca ggtccaccg tcaaagcca acgccacaa 
      cggtcacga ttgtgaaca atgaccaca gctcggaat 
      gctcgcacg atgtatgag gctgcgctt cctgcctct 
      actgttcca aatacagtt aaaggggca gaggccatt 
      gtaaatagc aaccgatta aagtgttac aaatttgtg 
      aatttgatg tgatgtttg cttgtaaat acttaatga 
      ttgtaaatt taatatgta ttttcctac ttgtttcaa 
      aagactgta aacgaatgt attttataa taatcaaac 
      agtacaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY