Status
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Gene information
General information
TC014730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14194799..14202426
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014730
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05683

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014730 RA
      MSCRVYSSS SSDWDINDS AIPRINEFD SFSEWADGH 
      CRLVYRADN EDAKRHSSG WAMRNTNNH NVHILKKSC 
      LGVLVCSLR CTLANGERV HLRPAICDK ARKKQQGKP 
      CPNRQCTGR LEILPCRGH CGYPVTHFW RHTDHAIFF 
      QAKGVHDHP RPEAKSTSE ARRSLGSGR RVRGLAVLL 
      AKEAALGNK LMSLREPKR QCRDLTSAR ALNPPPLIA 
      DQDKGYCSC PPFECICNY QPVLPTTLQ QYSPTYQQP 
      QVLDSYWTQ EPAQAQSYP AEVTLNQQY DFTNIPADL 
      FQPEEIFQL DQPIKPDFV SNAQSELNR SPSTLLDLG 
      SGTIHREFK SEEYWNQSL NTMINDDSN NSGSSRFNL 
      SQSPDNTLS LNNNLVPSI NQNSYVESA KMDELSYSV 
      QKATNYYQQ TMLDGEYNK TQNFEIITD NKVYFDENM 
      QHFETFPKT YKSNYQEKY IDLGQYTDY NNFLSAYDG 
      SKINDSAMF GDLDFRINN CVPGASNSH YSHENFDLI 
      THQ
    CDS sequence
    • >TC014730 RA
      atgtcgtgc cgggtctac tcctcatca tcctccgac 
      tgggacatc aacgacagc gcaatcccc cgcatcaac 
      gaattcgac tccttctcc gaatgggcc gatggccac 
      tgccgttta gtctaccgt gccgacaac gaagacgcc 
      aaacggcac tcctccggc tgggccatg cggaacacc 
      aacaaccac aacgtgcac atcctgaag aagagctgt 
      ctgggagtc ctcgtctgc tccctgcgc tgcaccctc 
      gccaacggc gagcgcgtg catctgcgg cccgccatc 
      tgcgacaag gcccggaag aagcaacaa ggaaagccg 
      tgtccgaat cggcagtgc accgggagg ctggagatc 
      ctcccctgt cggggacac tgcgggtat cccgttacg 
      catttctgg agacacacc gatcatgcg atcttcttc 
      caagcgaag ggagtgcac gatcatccc agaccggaa 
      gccaagagc acgtctgag gcgaggagg agtctgggg 
      agcggaaga agggtcaga ggattggcg gttttattg 
      gccaaggag gcagcgctc ggtaataag ctgatgtcg 
      ctgcgagag ccgaaacgg cagtgtcgc gatctgacc 
      tcagcccgc gccctcaac cctcctcca ctgatcgcg 
      gaccaagac aaaggttat tgttcttgc ccgcctttt 
      gagtgtatt tgtaattat cagccggtg cttcccaca 
      actttgcaa cagtactcc ccgacctac caacagccg 
      caggtcctg gactcctac tggacgcag gagccggcc 
      caggcccag tcctatcct gccgaagtg accctcaac 
      cagcagtac gacttcaca aacattccg gcagatctg 
      ttccaaccg gaagagatt ttccagctg gaccagccc 
      atcaagccg gactttgtg agcaacgcc cagagcgag 
      ctcaacagg tcgccttcc acgctcctg gatctgggc 
      agcgggacc atacaccgg gagttcaag tcggaggag 
      tattggaac caaagtctc aacaccatg atcaacgac 
      gacagcaac aacagcggc agcagccgg ttcaatcta 
      agtcaatcc cctgataac actctaagt cttaataac 
      aacctagtt ccttcaata aaccaaaac agttacgtg 
      gaaagtgcg aaaatggac gaactttcg tatagtgta 
      cagaaagct acgaactac taccagcag acgatgctc 
      gacggtgaa tacaacaaa acgcaaaac tttgaaatc 
      atcactgac aacaaggtc tattttgac gaaaacatg 
      caacacttt gaaactttt cccaagacg tacaagtcc 
      aactaccag gagaaatac atcgatttg gggcaatac 
      accgactat aataatttt ctgtcggct tatgatggc 
      agcaaaatc aatgacagt gccatgttt ggtgacctc 
      gatttccga attaataac tgtgtacca ggtgcgagt 
      aattcacac tacagtcac gaaaatttc gatctaatt 
      acacaccag taa
    mRNA sequence
    • >TC014730 RA
      aggtgttag ataccgaaa accgggagg tgtttcctg 
      aaatagacg cggctccta ttggccggc aaagcgtta 
      gattcccgg ggaagggat agagggagt ggcgttcta 
      ttgccagga atgaacatg cgttttcag gatcagtcg 
      tccttgcac ctttctaac gacagggtg tttcatgca 
      aacgcaccg gaactgtga cgtactgaa tgtgtaaca 
      agtgacacg gcgtgacca ctttgggat cttaatcgg 
      acatttccg gagatatgg taattctgc ataccccgt 
      cgtcccctg gcatgtcgt gccgggtct actcctcat 
      catcctccg actgggaca tcaacgaca gcgcaatcc 
      cccgcatca acgaattcg actccttct ccgaatggg 
      ccgatggcc actgccgtt tagtctacc gtgccgaca 
      acgaagacg ccaaacggc actcctccg gctgggcca 
      tgcggaaca ccaacaacc acaacgtgc acatcctga 
      agaagagct gtctgggag tcctcgtct gctccctgc 
      gctgcaccc tcgccaacg gcgagcgcg tgcatctgc 
      ggcccgcca tctgcgaca aggcccgga agaagcaac 
      aaggaaagc cgtgtccga atcggcagt gcaccggga 
      ggctggaga tcctcccct gtcggggac actgcgggt 
      atcccgtta cgcatttct ggagacaca ccgatcatg 
      cgatcttct tccaagcga agggagtgc acgatcatc 
      ccagaccgg aagccaaga gcacgtctg aggcgagga 
      ggagtctgg ggagcggaa gaagggtca gaggattgg 
      cggttttat tggccaagg aggcagcgc tcggtaata 
      agctgatgt cgctgcgag agccgaaac ggcagtgtc 
      gcgatctga cctcagccc gcgccctca accctcctc 
      cactgatcg cggaccaag acaaaggtt attgttctt 
      gcccgcctt ttgagtgta tttgtaatt atcagccgg 
      tgcttccca caactttgc aacagtact ccccgacct 
      accaacagc cgcaggtcc tggactcct actggacgc 
      aggagccgg cccaggccc agtcctatc ctgccgaag 
      tgaccctca accagcagt acgacttca caaacattc 
      cggcagatc tgttccaac cggaagaga ttttccagc 
      tggaccagc ccatcaagc cggactttg tgagcaacg 
      cccagagcg agctcaaca ggtcgcctt ccacgctcc 
      tggatctgg gcagcggga ccatacacc gggagttca 
      agtcggagg agtattgga accaaagtc tcaacacca 
      tgatcaacg acgacagca acaacagcg gcagcagcc 
      ggttcaatc taagtcaat cccctgata acactctaa 
      gtcttaata acaacctag ttccttcaa taaaccaaa 
      acagttacg tggaaagtg cgaaaatgg acgaacttt 
      cgtatagtg tacagaaag ctacgaact actaccagc 
      agacgatgc tcgacggtg aatacaaca aaacgcaaa 
      actttgaaa tcatcactg acaacaagg tctattttg 
      acgaaaaca tgcaacact ttgaaactt ttcccaaga 
      cgtacaagt ccaactacc aggagaaat acatcgatt 
      tggggcaat acaccgact ataataatt ttctgtcgg 
      cttatgatg gcagcaaaa tcaatgaca gtgccatgt 
      ttggtgacc tcgatttcc gaattaata actgtgtac 
      caggtgcga gtaattcac actacagtc acgaaaatt 
      tcgatctaa ttacacacc agtaactag gcagtcttt 
      tcacgtttt cgagtgcct tgaacactt atgctttca 
      atgatcttc attatgttt gagtattat tgttgtaca 
      cattgtata tactatgta taaataaat agagttgtg 
      atac
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