Status
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Gene information
General information
TC014717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13807922..13813020
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014717
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05682

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014717 RA
      MSSSEVGVE KNENAETKP PTPKKRGRK RKSEITVRQ 
      FEEEIDTSP KEETVSRRG RLRKKVNYY ELANPDNLE 
      YTLPVQTEI NYFSTSGNG EIKIKKKKV RHQEPDFSE 
      TSADSKESA KQKDEDNVL SNNEPQKDL FKSTNGKED 
      DSLLQDLVL EDSTTDFEA TTSTPNSKD KRYNYTGET 
      VKMMNKLFS LFANTSDDN STNNTSNGN NLPNSGKRS 
      GKRQRKDNM KEEDLDDGT GNVTCAICN STFDKNIWT 
      HHKQRYHNN LAWRVGDPP LDLNDQSFV MHTLNSLYK 
      KKKPLYCDK CGEVKKSVV GFLSHRSQC LKSGEQLES 
      VKISCDLCG RRMLPVSLT THMKMIHQN PDKEEVPEK 
      TTNNAAKPL PSKRSAAKK AMRIIENVT KVDNKDQYF 
      SANLDLGSQ QFAKDLMQK ELDETQFIE CKFKCNCIT 
      TSMEDALSH LNECPEKFS EGFVCKQCL LISSKLEDI 
      IYHIEFAHE INVTVDGDP SYVDKESNK MPSFLQSTV 
      RKTEKCLFP YAFDWTLKF CEENFSDLL FEHLKPNWP 
      LLKPETAAQ YLPKLEESC DVARIHVTC FTNTLKDSY 
      TYEKYKLFE SQIHDDHKI TLFCGGPIS AMSWLPTPY 
      TCLETSQVL AVSVLNSPD EKYETYQSY EVRSAIQFW 
      NFGQLRNTE ASLQKPELV FSLAHDYGP VWHMEWCPS 
      GCYNLEGGR MGLLAVTGS NSVVYIYSI PFFNQELGL 
      FYKPRPVIK LLLQRPGNS LLGGLKYYP CKISWSKSS 
      GHNFIAVGY TNGFVALFN LKTNSVILK YCDDDNVPC 
      ILPQKCFQA HSHAITALA LYHLNGGCR WLLSASVDR 
      AVNLWDLDD HSYPICTVK KNIITDGFW FNNWLCHVT 
      APDEGSTTA HVTTMLKQS RDYMFDPNV TLTCSPSTI 
      SAISGSDWL NGAVHANAV GEIVGIFPH QMLYTPDQK 
      ALKSKRMLF GYTTLIEKN TTGEERVAN DKIRKKTLQ 
      NLSTKKLTA GIKLSNIKS DFDHSSLFM NEPVLYEEA 
      EQKYALLFC DNKMESYDD FPKNVTKQL TALSKDYGI 
      TPPNQYPLN TINKIVFNP NRQASLYYA TGYQAGFVR 
      LSWLQFLKS DQQIK
    CDS sequence
    • >TC014717 RA
      atgtcgagt agcgaagtt ggtgttgaa aaaaatgaa 
      aacgctgaa accaagccc ccaacaccc aaaaaacga 
      ggaaggaaa agaaaatca gaaattact gtaagacag 
      tttgaagag gaaattgat acaagtcca aaggaagaa 
      actgtatca agaagggga cgcctacgt aaaaaagtg 
      aactattac gaattagct aatccagat aatctggaa 
      tatacacta cctgtccaa acggaaatc aactatttt 
      agtaccagc ggaaacggg gaaataaaa attaagaaa 
      aaaaaagtt aggcaccaa gaaccagat ttttccgaa 
      acttctgct gacagtaaa gaatcagcg aagcagaaa 
      gatgaagac aatgtttta agtaataat gagccgcaa 
      aaggattta tttaaatca acgaatggc aaagaagat 
      gatagtttg cttcaagac ttggttctt gaggactct 
      accacagat tttgaggca accacaagc actccgaac 
      tctaaggac aaaagatac aattacaca ggcgagact 
      gtgaaaatg atgaataaa ctgttcagt ttatttgca 
      aacactagt gatgataac agtacaaat aataccagc 
      aatggtaac aatttacca aacagtggt aaaaggtca 
      ggcaagaga caacgaaaa gataacatg aaagaggaa 
      gacttggat gacggcact ggcaacgtc acgtgtgca 
      atttgcaac agcacattt gacaaaaat atatggacc 
      caccataag cagagatat cataataat ttagcgtgg 
      agggttgga gacccacct ttagactta aacgaccaa 
      tcttttgta atgcacact ctcaactcg ttgtacaag 
      aaaaagaaa cctttatat tgtgataaa tgcggggaa 
      gtaaaaaaa tcagtagtt gggttttta tctcaccgc 
      tcccaatgt ttaaaatcg ggagaacag ctcgaatct 
      gttaaaatt tcttgcgac ttgtgcggg aggagaatg 
      ctaccagtg agtctaaca actcacatg aaaatgata 
      caccagaat ccggacaag gaggaggtt ccggaaaaa 
      acgacaaat aacgcggca aaaccgctt ccttccaaa 
      agaagtgcg gctaaaaaa gcaatgaga attattgaa 
      aacgttacg aaagttgat aataaggac cagtatttt 
      tcagcaaat ttagatcta ggttcacaa caatttgca 
      aaagacttg atgcagaaa gagctagac gagacacaa 
      tttatagaa tgtaaattc aagtgtaat tgtattaca 
      acaagcatg gaagatgcc ttgagtcat ttgaacgaa 
      tgtccggaa aaatttagt gaaggtttt gtttgcaag 
      caatgtttg ctgatttcg tccaaattg gaagacatt 
      atttatcac attgaattt gctcatgaa attaatgta 
      acagttgat ggcgacccc agttatgtg gacaaagaa 
      tccaacaaa atgccctca tttttgcaa agcacggtg 
      cgaaaaacc gaaaaatgc ctgttcccg tatgctttc 
      gactggacc ttaaaattt tgtgaggaa aatttctcc 
      gatttactt tttgagcat ttaaaacca aactggccg 
      ctccttaaa cccgagacg gccgctcaa tatctgcca 
      aaacttgag gagtcttgt gacgtggct cgaatccac 
      gtaacgtgt ttcacaaac acgctaaaa gactcgtac 
      acgtacgaa aaatacaaa ttatttgaa tcgcaaatt 
      cacgatgac cacaaaata accctgttt tgtgggggc 
      cctatttcg gcaatgtcc tggttgcca acgccttac 
      acgtgtttg gaaaccagt caggttttg gccgtttcg 
      gtacttaac tctcctgat gaaaaatac gagacttat 
      cagagttat gaagtaagg tctgcgatt cagttttgg 
      aatttcggt caacttcga aatacggaa gcttcgttg 
      caaaaacct gaattagtg tttagttta gcgcatgat 
      tacggccct gtgtggcac atggagtgg tgtccttca 
      gggtgttac aacttggaa ggggggcgg atggggctt 
      ttagcggtc acagggtca aactcagtg gtttatatt 
      tattcaatc ccatttttt aatcaagaa ttaggtttg 
      ttttacaaa cctagacct gtaataaag ttattatta 
      caaaggcca ggtaattcg ttactaggg ggactcaag 
      tattatcct tgtaaaatt tcatggagt aaatcttct 
      ggtcataat tttatagct gtgggttac acaaatggt 
      tttgtggcc ctttttaac ttgaaaaca aattccgta 
      atattgaaa tattgcgat gatgataat gttccatgt 
      attttgccc cagaagtgc ttccaggcg cattcgcac 
      gccatcaca gctcttgct ctctaccac ctcaacggg 
      ggctgccga tggctgctt tcagcttcc gtagaccgc 
      gctgtaaac ctctgggac ttggacgac cacagttat 
      cccatctgt acggtcaaa aagaacata attactgat 
      ggtttttgg ttcaataat tggctatgt catgtgaca 
      gcgcccgac gagggctca actacagcg catgttaca 
      acaatgctg aaacaatca cgcgattac atgttcgat 
      cctaatgtg acactaaca tgctcgccg tctacgatt 
      tcggcaatc tcgggtagt gattggctg aatggtgcc 
      gtccatgcc aacgcagta ggcgagatt gtggggatt 
      ttcccccac cagatgcta tacacacct gatcagaaa 
      gcgctgaaa tcgaaacga atgttgttt ggttacacc 
      accttgatt gagaaaaat acaacgggg gaggagcga 
      gtcgccaat gacaaaatc cgcaaaaag acgttgcaa 
      aatctgtcg acgaaaaaa ttgacggct gggataaaa 
      ttatccaat atcaagtcg gattttgac catagtagt 
      ctattcatg aacgagccg gtcttgtac gaagaggcc 
      gagcagaaa tacgctttg ttgttttgt gataataaa 
      atggaatct tacgatgat tttcctaaa aatgtgact 
      aaacagtta actgcgttg tcgaaagat tacgggata 
      acgccccca aatcagtac cctctaaat acaataaac 
      aaaattgtg ttcaatcct aaccgacaa gcgagtctg 
      tattacgca acgggatat caagccggg tttgtgaga 
      cttagttgg ttgcagttt ttaaaaagt gaccaacaa 
      attaagtag
    mRNA sequence
    • >TC014717 RA
      taacgctga aaaacactc attattaca gtcttggta 
      atgtagtcc ctgtaaata attaagtaa taaaaataa 
      ttattaaat aaatggaaa aaaaacctt taaaactaa 
      gcgccaact aagtgccaa cgtaacatg tttgccatg 
      ttttgatct tggcaacac ttttttcat tgaaatatc 
      cccaagata atgtcattt tctaataga aaaaaagtt 
      atttaattt ccatttagc acgtaaccg cagaacaat 
      ttttatatt taatacaaa aatagtttt tttgataag 
      caaactaaa gatacaaaa aggcgcaaa gctgccgta 
      cattgaggt tatgtgaat gaactcgta atgttgaaa 
      aaaaagtaa ataagcaat aaataatct ttgaaaaaa 
      gtgcacgat gtcgagtag cgaagttgg tgttgaaaa 
      aaatgaaaa cgctgaaac caagccccc aacacccaa 
      aaaacgagg aaggaaaag aaaatcaga aattactgt 
      aagacagtt tgaagagga aattgatac aagtccaaa 
      ggaagaaac tgtatcaag aaggggacg cctacgtaa 
      aaaagtgaa ctattacga attagctaa tccagataa 
      tctggaata tacactacc tgtccaaac ggaaatcaa 
      ctattttag taccagcgg aaacgggga aataaaaat 
      taagaaaaa aaaagttag gcaccaaga accagattt 
      ttccgaaac ttctgctga cagtaaaga atcagcgaa 
      gcagaaaga tgaagacaa tgttttaag taataatga 
      gccgcaaaa ggatttatt taaatcaac gaatggcaa 
      agaagatga tagtttgct tcaagactt ggttcttga 
      ggactctac cacagattt tgaggcaac cacaagcac 
      tccgaactc taaggacaa aagatacaa ttacacagg 
      cgagactgt gaaaatgat gaataaact gttcagttt 
      atttgcaaa cactagtga tgataacag tacaaataa 
      taccagcaa tggtaacaa tttaccaaa cagtggtaa 
      aaggtcagg caagagaca acgaaaaga taacatgaa 
      agaggaaga cttggatga cggcactgg caacgtcac 
      gtgtgcaat ttgcaacag cacatttga caaaaatat 
      atggaccca ccataagca gagatatca taataattt 
      agcgtggag ggttggaga cccaccttt agacttaaa 
      cgaccaatc ttttgtaat gcacactct caactcgtt 
      gtacaagaa aaagaaacc tttatattg tgataaatg 
      cggggaagt aaaaaaatc agtagttgg gtttttatc 
      tcaccgctc ccaatgttt aaaatcggg agaacagct 
      cgaatctgt taaaatttc ttgcgactt gtgcgggag 
      gagaatgct accagtgag tctaacaac tcacatgaa 
      aatgataca ccagaatcc ggacaagga ggaggttcc 
      ggaaaaaac gacaaataa cgcggcaaa accgcttcc 
      ttccaaaag aagtgcggc taaaaaagc aatgagaat 
      tattgaaaa cgttacgaa agttgataa taaggacca 
      gtatttttc agcaaattt agatctagg ttcacaaca 
      atttgcaaa agacttgat gcagaaaga gctagacga 
      gacacaatt tatagaatg taaattcaa gtgtaattg 
      tattacaac aagcatgga agatgcctt gagtcattt 
      gaacgaatg tccggaaaa atttagtga aggttttgt 
      ttgcaagca atgtttgct gatttcgtc caaattgga 
      agacattat ttatcacat tgaatttgc tcatgaaat 
      taatgtaac agttgatgg cgaccccag ttatgtgga 
      caaagaatc caacaaaat gccctcatt tttgcaaag 
      cacggtgcg aaaaaccga aaaatgcct gttcccgta 
      tgctttcga ctggacctt aaaattttg tgaggaaaa 
      tttctccga tttactttt tgagcattt aaaaccaaa 
      ctggccgct ccttaaacc cgagacggc cgctcaata 
      tctgccaaa acttgagga gtcttgtga cgtggctcg 
      aatccacgt aacgtgttt cacaaacac gctaaaaga 
      ctcgtacac gtacgaaaa atacaaatt atttgaatc 
      gcaaattca cgatgacca caaaataac cctgttttg 
      tgggggccc tatttcggc aatgtcctg gttgccaac 
      gccttacac gtgtttgga aaccagtca ggttttggc 
      cgtttcggt acttaactc tcctgatga aaaatacga 
      gacttatca gagttatga agtaaggtc tgcgattca 
      gttttggaa tttcggtca acttcgaaa tacggaagc 
      ttcgttgca aaaacctga attagtgtt tagtttagc 
      gcatgatta cggccctgt gtggcacat ggagtggtg 
      tccttcagg gtgttacaa cttggaagg ggggcggat 
      ggggctttt agcggtcac agggtcaaa ctcagtggt 
      ttatattta ttcaatccc attttttaa tcaagaatt 
      aggtttgtt ttacaaacc tagacctgt aataaagtt 
      attattaca aaggccagg taattcgtt actaggggg 
      actcaagta ttatccttg taaaatttc atggagtaa 
      atcttctgg tcataattt tatagctgt gggttacac 
      aaatggttt tgtggccct ttttaactt gaaaacaaa 
      ttccgtaat attgaaata ttgcgatga tgataatgt 
      tccatgtat tttgcccca gaagtgctt ccaggcgca 
      ttcgcacgc catcacagc tcttgctct ctaccacct 
      caacggggg ctgccgatg gctgctttc agcttccgt 
      agaccgcgc tgtaaacct ctgggactt ggacgacca 
      cagttatcc catctgtac ggtcaaaaa gaacataat 
      tactgatgg tttttggtt caataattg gctatgtca 
      tgtgacagc gcccgacga gggctcaac tacagcgca 
      tgttacaac aatgctgaa acaatcacg cgattacat 
      gttcgatcc taatgtgac actaacatg ctcgccgtc 
      tacgatttc ggcaatctc gggtagtga ttggctgaa 
      tggtgccgt ccatgccaa cgcagtagg cgagattgt 
      ggggatttt cccccacca gatgctata cacacctga 
      tcagaaagc gctgaaatc gaaacgaat gttgtttgg 
      ttacaccac cttgattga gaaaaatac aacggggga 
      ggagcgagt cgccaatga caaaatccg caaaaagac 
      gttgcaaaa tctgtcgac gaaaaaatt gacggctgg 
      gataaaatt atccaatat caagtcgga ttttgacca 
      tagtagtct attcatgaa cgagccggt cttgtacga 
      agaggccga gcagaaata cgctttgtt gttttgtga 
      taataaaat ggaatctta cgatgattt tcctaaaaa 
      tgtgactaa acagttaac tgcgttgtc gaaagatta 
      cgggataac gcccccaaa tcagtaccc tctaaatac 
      aataaacaa aattgtgtt caatcctaa ccgacaagc 
      gagtctgta ttacgcaac gggatatca agccgggtt 
      tgtgagact tagttggtt gcagttttt aaaaagtga 
      ccaacaaat taagtaggt tttgttgta atttatttt 
      gtaaataaa tttttggaa ctgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY