Status
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Gene information
General information
TC014712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13759101..13760810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014712
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05679

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014712 RA
      MKSFECESS SEGDEPTIH PISRTIAQP PQWLRGNSA 
PGWGWQTGC GSSTGGPSS SSGALDQTN FAPFTSTEE 
GQIPGRRTP LGAVGLGGF YFQGCQPNI ASHGPPSDE 
NTPDPTDST QPNRPQHSR ASQNANPSK AKNRQGKSV 
RLNINARER RRMHDLNDA LDELRAVIP YAHSPSVRK 
LSKIATLLL AKNYILMQA NALEEMKRL IAYLQGTAG 
GAAIVAPPG FDLQGFPAA AKFLQQQQA LQAENARNS 
EGNNGSSGS AM
    CDS sequence
    • >TC014712 RA
      atgaaatca ttcgaatgt gaatcgtct tcagaaggc 
gacgaaccc accatccac cccatctca cggacgata 
gcgcagcct ccgcagtgg ctgcgcggg aactctgcc 
cccggctgg gggtggcaa actggctgc ggctcctca 
accggtggt ccgagctcc tcatccggc gccttagat 
caaacgaat ttcgctccc tttacctca accgaagag 
ggtcaaatc cccggcaga cgcacccct ttaggagcc 
gtgggatta ggcggcttc tacttccaa ggctgtcaa 
cccaacatc gctagccac gggccaccc tccgacgag 
aacaccccc gacccgacc gactccacc caacccaac 
cggccgcaa cattcccga gcgtcccaa aacgccaat 
ccctccaaa gcgaagaac agacaggga aaatccgtc 
cgattgaac ataaacgca agagaaagg cggcgaatg 
cacgacttg aacgacgct ttggacgaa cttagagct 
gtgatcccg tacgcacat tcgccctca gtcaggaaa 
ctgtccaag attgccaca ctgctcctg gccaagaac 
tacattcta atgcaagcc aacgctctg gaagagatg 
aagaggctg attgcctac ttgcagggg actgcagga 
ggagcggct attgtggcg ccgccaggg ttcgacctg 
cagggcttt cctgccgcc gccaagttc ctgcaacag 
caacaggcc ctccaagcc gaaaatgcc aggaacagt 
gagggcaac aacggaagc tcgggctcc gctatgtga
    mRNA sequence
    • >TC014712 RA
      gtctagttc aacaactag tgttgtagt ggtttagta 
tacaatgac aaacacacc aaacattta aaccaaaat 
aataaaaat ctcagtgac gaaactaaa atcatttta 
atacgaaaa attaccaaa aggcagtga tagtgattt 
aaggcaaca aattatgtg tgtttactt gtgattcgt 
ttacaactt aagtataca gtgcccagc tacgaaata 
atccaacaa caaatagta tgagaaagt tttatttaa 
accattgaa ttacataat tagtctata atacaaaaa 
atgttaaaa tgaaagtga gctgaaaga aatttccga 
aattaaacc aaattaaat cacgtaaaa atagtcccc 
ggaattgtg aatttaaaa accatgaaa tcattcgaa 
tgtgaatcg tcttcagaa ggcgacgaa cccaccatc 
caccccatc tcacggacg atagcgcag cctccgcag 
tggctgcgc gggaactct gcccccggc tgggggtgg 
caaactggc tgcggctcc tcaaccggt ggtccgagc 
tcctcatcc ggcgcctta gatcaaacg aatttcgct 
ccctttacc tcaaccgaa gagggtcaa atccccggc 
agacgcacc cctttagga gccgtggga ttaggcggc 
ttctacttc caaggctgt caacccaac atcgctagc 
cacgggcca ccctccgac gagaacacc cccgacccg 
accgactcc acccaaccc aaccggccg caacattcc 
cgagcgtcc caaaacgcc aatccctcc aaagcgaag 
aacagacag ggaaaatcc gtccgattg aacataaac 
gcaagagaa aggcggcga atgcacgac ttgaacgac 
gctttggac gaacttaga gctgtgatc ccgtacgca 
cattcgccc tcagtcagg aaactgtcc aagattgcc 
acactgctc ctggccaag aactacatt ctaatgcaa 
gccaacgct ctggaagag atgaagagg ctgattgcc 
tacttgcag gggactgca ggaggagcg gctattgtg 
gcgccgcca gggttcgac ctgcagggc tttcctgcc 
gccgccaag ttcctgcaa cagcaacag gccctccaa 
gccgaaaat gccaggaac agtgagggc aacaacgga 
agctcgggc tccgctatg tgaatgaaa gatgctcaa 
gttttataa tttcggcaa aaactccaa aaattacta 
gagaatagg cgatagatt ttaaaaatc gtgaaagtc 
tagctttta ttagtagaa cctagtcgt aacttttaa 
gatttttat ttaacggta gacgcgaac aaagaaact 
aaagcaata ttaaagtaa acgttgtag caataatac 
tgataaaga ttttttaac gttaacaat catagcact 
caactgcta aatgtccag aaattctag tcaattgta 
attagtgat gatgtgtaa tgtaaaata aaccgatag 
atacgtttg taaaatgaa atagagaaa taaagttta 
tgtccacag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY