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Gene information
General information
TC014695 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13514460..13517749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014695
    iBeetle screen iB_05676

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014695 RA
      MSWIFGYGR RDQQPPDMS QFGAPPAGG SGGAGGDIP 
      NDPKLTKAE RKQMEAYRF DSTALERAA QAAKELERS 
      SHAKEALEL SKLQETTRQ VEQQAKIKE YEAHIAQMQ 
      IEAKRAEAE EKRKLLVEE TKQHQARAQ YQDQLARKR 
      YDDQLAQQQ RMNEENLKR QEESVAKQE AMRKATIEH 
      EMELRHKNE MKRVEAELK AKAKVDREN RDLTLEQIR 
      LKATENRVT VLESIKTAG SVLGTGVHA LLTDWDKVL 
      TAAGGLSLL ALGVYSAKG ATSVTARYI EARLGKPSL 
      VRETSRFSF LDTIKHPIE AIKKIRTKQ QDALSGVVL 
      APQLEERLR DIAIATKNT KQNRGMYRN ILMHGPPGT 
      GKTMFAKRL AKHSGMDYA ILTGGDVAP MGRDGVTAI 
      HKVFDWAHS TRKGLLLFV DEADAFLRK RSSEHISED 
      LRATLNAFL YRSGEQSQK FMLVLASNT PEQFDWAVN 
      DRLDEMVQF GLPGLEERE RLIRLYFDK FVLEPATEG 
      KRRLKVDNF DYGALCSQM ARMTKGMSG REIAKLGVA 
      WQAAAYASE DGVLTEKMV LDRCRDAVK QHRQKVEWQ 
      SEQEKKESK SIYHSEKED SYSPVVPKQ KASQAEEEE 
      VDTAKVR
    CDS sequence
    • >TC014695 RA
      atgtcgtgg attttcggt tacggtaga cgcgaccag 
      cagccccct gacatgtcc caattcggg gcgcccccg 
      gccggtggg agtggaggt gccggtggt gacatcccc 
      aacgatcca aaactgacc aaagctgaa agaaagcaa 
      atggaagct tacagattc gactcgacg gctttggaa 
      cgtgcggcc caagccgca aaagagctc gagagatcg 
      tcacatgcg aaggaagcg ctcgaattg tccaagttg 
      caagaaacc accagacag gtcgagcag caggcgaaa 
      atcaaggag tatgaggcg cacattgct cagatgcaa 
      attgaggcc aaaagggcc gaagccgag gagaagagg 
      aagctgctg gtggaggag acgaagcag caccaagcg 
      agggcgcag taccaggac cagttggct aggaagcga 
      tatgacgac cagctcgcc caacaacaa aggatgaac 
      gaagagaat ttgaagaga caggaggag tccgttgcc 
      aaacaagag gccatgagg aaggccacc attgagcac 
      gagatggag ctgagacac aagaacgaa atgaaaagg 
      gttgaggct gaactgaag gccaaagct aaagttgac 
      agggaaaat cgcgacttg actcttgaa caaatcagg 
      ctcaaagcc accgaaaat cgcgtcacg gtgctcgag 
      agtataaaa acggcaggg tctgttttg gggacaggt 
      gtccacgcc ttgctcaca gactgggac aaggtttta 
      acagctgcg ggtgggttg tccctcctg gctttgggt 
      gtttattca gccaaaggg gcaactagt gtcacagca 
      cgttacata gaagcacgg ctaggaaaa ccatcgttg 
      gttagagaa acatcaaga ttttcgttt ctcgacaca 
      ataaagcat cccatcgaa gcaataaaa aaaattcga 
      acaaaacaa caagacgct ctgtctggt gtagtttta 
      gccccgcag ttggaggaa cgtctcaga gacattgct 
      attgctaca aaaaatacc aaacaaaat aggggcatg 
      tacaggaat attctaatg catggccct cctggtaca 
      ggaaaaacc atgtttgca aaacgatta gctaaacat 
      tcagggatg gactatgcc attttgaca gggggcgat 
      gtggccccc atgggtcgg gatggggtg acagctatc 
      cataaagtc tttgattgg gctcacagt accaggaaa 
      ggtttgttg ctgttcgtg gacgaggcg gacgcattt 
      ttgcgcaaa cgttcatcg gagcacatt tctgaagat 
      ctgcgcgcc acacttaac gcatttctg taccgatct 
      ggcgaacaa agtcaaaaa tttatgtta gttcttgcc 
      tcaaacact ccggagcag tttgattgg gcggtgaat 
      gaccgtctt gatgaaatg gtacagttt ggtttgccc 
      ggtttggag gaacgcgag cgtttaatt cggctctat 
      tttgataaa ttcgttttg gagcctgcg actgagggc 
      aaacggagg ttgaaagtt gataatttc gattacggt 
      gcgttgtgt agccaaatg gcacggatg accaaaggc 
      atgtctggc cgagaaatc gcaaaattg ggggtggca 
      tggcaggcg gcggcctac gcgtctgag gatggtgtt 
      ctgaccgag aaaatggtc ttggatagg tgtagagac 
      gcagtgaaa caacacaga caaaaggtc gagtggcaa 
      tccgaacag gaaaagaag gagtcgaag agtatatat 
      cattctgaa aaggaagat tcctattca cctgtagtg 
      ccaaagcag aaagctagc caagcggag gaagaggag 
      gtggataca gcgaaagtt agatag
    mRNA sequence
    • >TC014695 RA
      ccggtcgtt aaagactag ggatttttt atgcttttt 
      tggttattg caatagttg aggcaaaaa tcaacaatg 
      atgtaatgt ttgcggaag aaataacac aaataaacg 
      taacaatta tcactttat ttcgaaaat aaaaaatca 
      aaaaaaata ttttcaaaa tttttttgg cacaaaaga 
      ccacatgag cacaagcta taaaaaaga aaagtgttc 
      aaagatgca aaaaattgt aagtggaca caatctcag 
      cgataagca tttggtttg gaggacaat aaattatta 
      actcgtagt gattaagaa aaggttaaa ttaataaaa 
      aaaacgttt ttgtccaca tgcgatgtt atgcaactt 
      tggcatcta gaaaatacg tgcgcatgg tcgtgccct 
      cgttccttt gacagccgt ctttaacct aagaattgt 
      gtcacgttt tttctttga tgaaaattt gatagaaaa 
      taattgcgc aaaatgtcg tggattttc ggttacggt 
      agacgcgac cagcagccc cctgacatg tcccaattc 
      ggggcgccc ccggccggt gggagtgga ggtgccggt 
      ggtgacatc cccaacgat ccaaaactg accaaagct 
      gaaagaaag caaatggaa gcttacaga ttcgactcg 
      acggctttg gaacgtgcg gcccaagcc gcaaaagag 
      ctcgagaga tcgtcacat gcgaaggaa gcgctcgaa 
      ttgtccaag ttgcaagaa accaccaga caggtcgag 
      cagcaggcg aaaatcaag gagtatgag gcgcacatt 
      gctcagatg caaattgag gccaaaagg gccgaagcc 
      gaggagaag aggaagctg ctggtggag gagacgaag 
      cagcaccaa gcgagggcg cagtaccag gaccagttg 
      gctaggaag cgatatgac gaccagctc gcccaacaa 
      caaaggatg aacgaagag aatttgaag agacaggag 
      gagtccgtt gccaaacaa gaggccatg aggaaggcc 
      accattgag cacgagatg gagctgaga cacaagaac 
      gaaatgaaa agggttgag gctgaactg aaggccaaa 
      gctaaagtt gacagggaa aatcgcgac ttgactctt 
      gaacaaatc aggctcaaa gccaccgaa aatcgcgtc 
      acggtgctc gagagtata aaaacggca gggtctgtt 
      ttggggaca ggtgtccac gccttgctc acagactgg 
      gacaaggtt ttaacagct gcgggtggg ttgtccctc 
      ctggctttg ggtgtttat tcagccaaa ggggcaact 
      agtgtcaca gcacgttac atagaagca cggctagga 
      aaaccatcg ttggttaga gaaacatca agattttcg 
      tttctcgac acaataaag catcccatc gaagcaata 
      aaaaaaatt cgaacaaaa caacaagac gctctgtct 
      ggtgtagtt ttagccccg cagttggag gaacgtctc 
      agagacatt gctattgct acaaaaaat accaaacaa 
      aataggggc atgtacagg aatattcta atgcatggc 
      cctcctggt acaggaaaa accatgttt gcaaaacga 
      ttagctaaa cattcaggg atggactat gccattttg 
      acagggggc gatgtggcc cccatgggt cgggatggg 
      gtgacagct atccataaa gtctttgat tgggctcac 
      agtaccagg aaaggtttg ttgctgttc gtggacgag 
      gcggacgca tttttgcgc aaacgttca tcggagcac 
      atttctgaa gatctgcgc gccacactt aacgcattt 
      ctgtaccga tctggcgaa caaagtcaa aaatttatg 
      ttagttctt gcctcaaac actccggag cagtttgat 
      tgggcggtg aatgaccgt cttgatgaa atggtacag 
      tttggtttg cccggtttg gaggaacgc gagcgttta 
      attcggctc tattttgat aaattcgtt ttggagcct 
      gcgactgag ggcaaacgg aggttgaaa gttgataat 
      ttcgattac ggtgcgttg tgtagccaa atggcacgg 
      atgaccaaa ggcatgtct ggccgagaa atcgcaaaa 
      ttgggggtg gcatggcag gcggcggcc tacgcgtct 
      gaggatggt gttctgacc gagaaaatg gtcttggat 
      aggtgtaga gacgcagtg aaacaacac agacaaaag 
      gtcgagtgg caatccgaa caggaaaag aaggagtcg 
      aagagtata tatcattct gaaaaggaa gattcctat 
      tcacctgta gtgccaaag cagaaagct agccaagcg 
      gaggaagag gaggtggat acagcgaaa gttagatag 
      attatagcg accaaatag ggtaacaca atcgtactc 
      ttgtagttt taaattggc aatcccgca aacacagaa 
      ctgacccag tcactgccg tttatcagt tctgtgttt 
      gccgcccag taaactaaa ttaaacggt ttaagttta 
      ctgactgtt gtttatatt tatacaagg aaagtgagt 
      tcaccacca actggtgtg catttaatt cgccaaatt 
      tcggttgtt gttaaatta ttgcattgg ttttttata 
      tttatatgc aatttttta agggtctac ttctcgtgg 
      ttttgttca attgtgata ttgttgagt gcgtcttag 
      ggaagttta tattaaagt tctccgcat tcaaggtca 
      agattttgt tgctgtttt taaaattgt ccaatgtaa 
      aatactgta gataattgg acttccttt aataaacac 
      tttttacaa cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY