Status
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Gene information
General information
TC014687 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13464026..13466060
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014687
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05674

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014687 RA
      MKFILLVLF NLVSTFANQ DLNPEISTP LGKIQGSTL 
      VSRLNETIF AFRGVRYAQ PPIGDLRFK PPVPVNKWS 
      GVYNATSDG PVCPQPTDD PVSEDCLLL NVYTTELPD 
      NDNKPKRPV IVYLHPGGF YSVTGRSDW AGPQYFTDH 
      DIVLVTFNY RLGSLGFIS TGEDAPGNN GLKDQVLAL 
      KWVKNYIEY FGGDPDMVT LFGYSAGSW SITLHLVSP 
      MSSGLFHKA IIGSGSVLG QWKLPRNQL ELAKKQARL 
      VGCPVLNPK DMIACLKSI PAEVLGNSL DGFDEFSYD 
      PVIVWYPVI EDGFGQERF LTEDPIKMF ERGDFQKVP 
      IVIGVTKDE FGNRAFPIL LNATLTNEL SNDFETYAP 
      ICFLYERET NKSKEISAA LREFYLGND PLTNSSLQG 
      IADAFADSI IGFAANRAA RLISLKNSE PVYYYEFTY 
      QGRFSHFYL PDSNNTIPY GVVHHDDLI YLFYISAMF 
      PFFDDSYPE SEMVDKLTL MWSTFAKTG YPIPETGTI 
      LAGINWEPF NISSNKYLE IGKEIVLKE GLYQERYNF 
      WNELFPIRK LCF
    CDS sequence
    • >TC014687 RA
      atgaagttc atacttctt gtattattt aatttagtt 
      tcaactttt gcaaatcag gacctgaat cctgaaata 
      tctacacca ctcggcaag atacaaggt tccaccctt 
      gtctcaaga ttaaacgaa actattttt gcctttaga 
      ggagtgcgt tatgcacaa ccacctatc ggagatctt 
      cgttttaaa cccccagtc ccggtaaac aaatggtca 
      ggtgtttac aatgccact agtgacggt cctgtttgt 
      cctcaacca accgatgat cctgtttct gaagactgt 
      cttcttcta aatgtctac accactgaa ctccctgat 
      aatgacaat aagccaaaa cgccccgtc attgtctac 
      ttacaccca ggtggtttt tacagtgtg acaggtcgt 
      agtgactgg gctgggcca caatatttc acggaccat 
      gatattgtt ttggtcact tttaattat cgactgggg 
      tctttaggt ttcattagc accggtgag gatgctcca 
      ggaaacaac ggattaaaa gaccaagtt ctggcctta 
      aaatgggtg aaaaattat attgaatat ttcggggga 
      gacccagat atggttact ttgtttggc tacagcgct 
      ggttcctgg agcatcact ctgcatctg gtatcaccc 
      atgtcaagt ggtttgttc cataaagcc ataattgga 
      agtggctca gttttgggg caatggaag ttacctcga 
      aatcagcta gaattagcc aaaaagcaa gcgagatta 
      gtgggttgt cctgtgctt aaccctaag gatatgatt 
      gcttgtttg aaatcaatt ccagcggaa gtgttagga 
      aattctttg gatgggttt gatgagttt tcgtacgat 
      cctgtaatt gtgtggtat ccggttatt gaggacggt 
      ttcgggcaa gaaaggttc ttaaccgaa gacccaatt 
      aaaatgttt gaaagagga gactttcaa aaagtccca 
      attgtaatt ggggtgaca aaggacgag tttggcaac 
      agggccttt cctattctt cttaatgct acactaaca 
      aatgagtta agcaatgat tttgaaact tatgccccg 
      atttgtttt ctgtacgaa cgtgaaaca aacaaatcc 
      aaagaaata agtgcagct ctgagagaa ttttatcta 
      ggaaatgat cctttaact aactcctct cttcagggc 
      attgcagac gcatttgct gattcaatt ataggtttt 
      gctgcaaac agagctgcc aggctaata tcgctaaaa 
      aattctgaa cccgtttac tattacgaa tttacttac 
      caaggccgt ttcagtcat ttttatctt cctgacagt 
      aataatacc attccgtat ggtgtggtt catcacgat 
      gatttgatt tatctgttc tatatctcg gcaatgttt 
      ccgtttttt gacgatagt tatcccgag agtgaaatg 
      gtggataaa ctgacttta atgtggtca acttttgcc 
      aaaactggt tatccgatt cctgagacg ggcaccatt 
      ctcgcaggg ataaattgg gaacccttt aatatatcc 
      agtaacaaa tatctggaa attggcaag gagattgtt 
      cttaaagag ggattgtat caggagagg tacaatttc 
      tggaatgaa ttatttccg ataagaaaa ttatgtttt 
      tga
    mRNA sequence
    • >TC014687 RA
      acaaatcta accatgaag ttcatactt cttgtatta 
      tttaattta gtttcaact tttgcaaat caggacctg 
      aatcctgaa atatctaca ccactcggc aagatacaa 
      ggttccacc cttgtctca agattaaac gaaactatt 
      tttgccttt agaggagtg cgttatgca caaccacct 
      atcggagat cttcgtttt aaaccccca gtcccggta 
      aacaaatgg tcaggtgtt tacaatgcc actagtgac 
      ggtcctgtt tgtcctcaa ccaaccgat gatcctgtt 
      tctgaagac tgtcttctt ctaaatgtc tacaccact 
      gaactccct gataatgac aataagcca aaacgcccc 
      gtcattgtc tacttacac ccaggtggt ttttacagt 
      gtgacaggt cgtagtgac tgggctggg ccacaatat 
      ttcacggac catgatatt gttttggtc acttttaat 
      tatcgactg gggtcttta ggtttcatt agcaccggt 
      gaggatgct ccaggaaac aacggatta aaagaccaa 
      gttctggcc ttaaaatgg gtgaaaaat tatattgaa 
      tatttcggg ggagaccca gatatggtt actttgttt 
      ggctacagc gctggttcc tggagcatc actctgcat 
      ctggtatca cccatgtca agtggtttg ttccataaa 
      gccataatt ggaagtggc tcagttttg gggcaatgg 
      aagttacct cgaaatcag ctagaatta gccaaaaag 
      caagcgaga ttagtgggt tgtcctgtg cttaaccct 
      aaggatatg attgcttgt ttgaaatca attccagcg 
      gaagtgtta ggaaattct ttggatggg tttgatgag 
      ttttcgtac gatcctgta attgtgtgg tatccggtt 
      attgaggac ggtttcggg caagaaagg ttcttaacc 
      gaagaccca attaaaatg tttgaaaga ggagacttt 
      caaaaagtc ccaattgta attggggtg acaaaggac 
      gagtttggc aacagggcc tttcctatt cttcttaat 
      gctacacta acaaatgag ttaagcaat gattttgaa 
      acttatgcc ccgatttgt tttctgtac gaacgtgaa 
      acaaacaaa tccaaagaa ataagtgca gctctgaga 
      gaattttat ctaggaaat gatccttta actaactcc 
      tctcttcag ggcattgca gacgcattt gctgattca 
      attataggt tttgctgca aacagagct gccaggcta 
      atatcgcta aaaaattct gaacccgtt tactattac 
      gaatttact taccaaggc cgtttcagt catttttat 
      cttcctgac agtaataat accattccg tatggtgtg 
      gttcatcac gatgatttg atttatctg ttctatatc 
      tcggcaatg tttccgttt tttgacgat agttatccc 
      gagagtgaa atggtggat aaactgact ttaatgtgg 
      tcaactttt gccaaaact ggttatccg attcctgag 
      acgggcacc attctcgca gggataaat tgggaaccc 
      tttaatata tccagtaac aaatatctg gaaattggc 
      aaggagatt gttcttaaa gagggattg tatcaggag 
      aggtacaat ttctggaat gaattattt ccgataaga 
      aaattatgt ttttgaaat tttgactgg agaattgta 
      ttgtaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY