| General information | |||
| TC033225
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13394366..13416135 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033225 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05673 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
Pupal morphology
antenna unilateral orientation irregular -
Number of animals showing the phenotype: 2
wings shortened - Number of animals showing the phenotype: 3
wings shortened - Number of animals showing the phenotype: 3
- >TC033225 RA
MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT QAPKNLVTQ TSLQRKKSL PDFQDLPRA TGAMSREEV SYLGSARRE EVRRQAEEK ERLRANPLL YLVSPQVKD WLSRQQLVI VVLFINISL AIMFFKLLT
- >TC033225 RB
MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT QAPKDLPRA TGAMSREEV SYLGSARRE EVRRQAEEK ERLRANPLL YLVSPQVKD WLSRQQLVI VVLFINISL AIMFFKLLT
- >TC033225 RC
MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT QAPKQCSRR LWRTSCTML WYQDDDEEE NLMFSPALM ARRASESWI DTPPVENLV TQTSLQRKK SLPDFQDLP RATGAMSRE EVSYLGSAR REEVRRQAE EKERLRANP LLYLVSPQV KDWLSRQQL VIVVLFINI SLAIMFFKL LT
- >TC033225 RA
atgcaacag gcgaaccag gtgggggac aacgggggc tcctccccc gcggccacc ggggccacg ggcacggcc accacccgc agccacgtg aacggcggc cgcttcgac ttcgacgac ggcggcacc tactgcggc ggctgggag gacggcaag gcccacgga cacggcgtc tgcaccggc cccaagggc cagggggcc tactccggc tcctggcac tacggcttc gaagtctcc ggggtctat acttggccg agtgggagt tgcttcgaa gggcaatgg caaaatgga aaaagacac ggcttcggg gtggaaacc cgaggccgg tggatttat cggggagag tggacgcag ggctttaaa ggacgatat ggagtgcgg cagtccaac acctccaca gccaaatac gaaggaact tgggcgaat ggcctacaa gatggttac ggatccgag acttacgca gacgatggc acttaccaa ggacaatgg ctgagaggc atgagacat ggctacggc gtaaggaac agtgcgcca ttcggcaaa gcgtccaaa tacagagct aacaagaca ttaagggcg tctctaacg tcgctacgc agtaatgaa ggcaatacg ggagcaaat ccagaagcg gcggaaaaa agagaccgg cgagttgat gattccaga ggaggcttc gtccttaaa atgaactcg gccgaaccc acgaaaagg agacaatcc ctcggctcc ggaaatatt aagaaagga ttgcttatg gggttgaag ttgaagaag caacgaagc actggcgat ttagaaaaa aggggtggt ggaggatct ggcagtatc agatcgaca ggatcatca gcctcttgg gtcagcact gattcggca cagtcaggc atgaccaac gcttctata ccgtccgat tccaatgcg agcttcgtt gttgaggac gagcaaatg gatcctaaa gtaaccgaa acttacatg ggtgagtgg aagaacgac aaaagatca ggcttcggc attagcgag agatccgat ggtctcaaa tacgaaggc gaatggtat gcgaacaag aaatacggt tacggtgta accacgttc agtaacggg gagaaagag gaaggcaaa tacaagaac aacgtcctt ataacgagc caaaagaag aaactgttt ttaatgaga tcagctaaa tttagggaa agaatcgac gccgcagtg aacgccgct caaagagca tcgaaaata gcgttacag aaagcagat attgcgata tcgagaacg gcaacggcc agaggcaaa gccgagcaa gcggaccta gcggctgct caggcgagg gaagactcg gacgtggcc acaactgtt gccaaacaa tacgcgccc gacttcaag cagccgggt ctggaaagg ctgaaagcg cgacaaaac aagtaccaa atcaacaca gacacagtc accctcaac cagaaggag aacccacac aaccaaatt ccaaaccaa gtaccgaac caaattccc aaccaaatt cccaatcaa acgccaaac caagtcaac aaaccactc gcgaaccaa ctcaaccag ataccgaac caaataccc aacaaacca cacaaccaa accaactac ccgccccag caaaccgta ggtttcaac ctccaacaa aaccaaaac gcttataac aacactaac aatgtgtcc aacgagttc ggtccgcag caaaacttc tcaccggac aataaaatc accaacgaa ttccccgga aacaaaccc cggaattcc ctcccagcc gacgacatc tccccgcaa gtgagctcg ctccggagg ggctcgagg cgggtttcc ggcgatagg ccgtactta gggaactta ggtcaacag tcttcgata gatcacttc gatcactac aaaaggccg cccagtcgg gactcgagt gtggatagg tactcccga gccacaggc aggatgagc agcacagtc gggtcccga cagccctca gtggaacgg tcgtctcgt ccttcggag acccccgac aggtccttg cgggcgccg tcggtcgca agggggacg actccggtt ccgacgatt cccaacacg aagaacggg tccgtgttg accggaagt gcggcggga agcaacgcc aagttggct gggaataat ccaggacag ccgccgccg ttcgaagaa atcatcatt cggaagcgg ggactgggc caggatatc attccttct cctaaccaa ccgaaacgg actgaaagc ctattcatt cctggagcg ccaacgacg caagcgcct aagaatctg gtgacgcag acttcgttg caacgcaag aaatcactg cccgatttt caggatctg ccgagggcg accggtgcc atgtcgagg gaggaggtg tcctatctt gggtcagct cgcagagag gaggtccgc cgtcaagcc gaagagaaa gaacgactt cgtgccaat ccgctcctc tacctagtc agtccgcaa gtaaaggat tggctctcg cgccaacag ctggtgatt gtagtgctg ttcatcaat atatcgctg gccatcatg ttcttcaag ctgctgaca tag
- >TC033225 RB
atgcaacag gcgaaccag gtgggggac aacgggggc tcctccccc gcggccacc ggggccacg ggcacggcc accacccgc agccacgtg aacggcggc cgcttcgac ttcgacgac ggcggcacc tactgcggc ggctgggag gacggcaag gcccacgga cacggcgtc tgcaccggc cccaagggc cagggggcc tactccggc tcctggcac tacggcttc gaagtctcc ggggtctat acttggccg agtgggagt tgcttcgaa gggcaatgg caaaatgga aaaagacac ggcttcggg gtggaaacc cgaggccgg tggatttat cggggagag tggacgcag ggctttaaa ggacgatat ggagtgcgg cagtccaac acctccaca gccaaatac gaaggaact tgggcgaat ggcctacaa gatggttac ggatccgag acttacgca gacgatggc acttaccaa ggacaatgg ctgagaggc atgagacat ggctacggc gtaaggaac agtgcgcca ttcggcaaa gcgtccaaa tacagagct aacaagaca ttaagggcg tctctaacg tcgctacgc agtaatgaa ggcaatacg ggagcaaat ccagaagcg gcggaaaaa agagaccgg cgagttgat gattccaga ggaggcttc gtccttaaa atgaactcg gccgaaccc acgaaaagg agacaatcc ctcggctcc ggaaatatt aagaaagga ttgcttatg gggttgaag ttgaagaag caacgaagc actggcgat ttagaaaaa aggggtggt ggaggatct ggcagtatc agatcgaca ggatcatca gcctcttgg gtcagcact gattcggca cagtcaggc atgaccaac gcttctata ccgtccgat tccaatgcg agcttcgtt gttgaggac gagcaaatg gatcctaaa gtaaccgaa acttacatg ggtgagtgg aagaacgac aaaagatca ggcttcggc attagcgag agatccgat ggtctcaaa tacgaaggc gaatggtat gcgaacaag aaatacggt tacggtgta accacgttc agtaacggg gagaaagag gaaggcaaa tacaagaac aacgtcctt ataacgagc caaaagaag aaactgttt ttaatgaga tcagctaaa tttagggaa agaatcgac gccgcagtg aacgccgct caaagagca tcgaaaata gcgttacag aaagcagat attgcgata tcgagaacg gcaacggcc agaggcaaa gccgagcaa gcggaccta gcggctgct caggcgagg gaagactcg gacgtggcc acaactgtt gccaaacaa tacgcgccc gacttcaag cagccgggt ctggaaagg ctgaaagcg cgacaaaac aagtaccaa atcaacaca gacacagtc accctcaac cagaaggag aacccacac aaccaaatt ccaaaccaa gtaccgaac caaattccc aaccaaatt cccaatcaa acgccaaac caagtcaac aaaccactc gcgaaccaa ctcaaccag ataccgaac caaataccc aacaaacca cacaaccaa accaactac ccgccccag caaaccgta ggtttcaac ctccaacaa aaccaaaac gcttataac aacactaac aatgtgtcc aacgagttc ggtccgcag caaaacttc tcaccggac aataaaatc accaacgaa ttccccgga aacaaaccc cggaattcc ctcccagcc gacgacatc tccccgcaa gtgagctcg ctccggagg ggctcgagg cgggtttcc ggcgatagg ccgtactta gggaactta ggtcaacag tcttcgata gatcacttc gatcactac aaaaggccg cccagtcgg gactcgagt gtggatagg tactcccga gccacaggc aggatgagc agcacagtc gggtcccga cagccctca gtggaacgg tcgtctcgt ccttcggag acccccgac aggtccttg cgggcgccg tcggtcgca agggggacg actccggtt ccgacgatt cccaacacg aagaacggg tccgtgttg accggaagt gcggcggga agcaacgcc aagttggct gggaataat ccaggacag ccgccgccg ttcgaagaa atcatcatt cggaagcgg ggactgggc caggatatc attccttct cctaaccaa ccgaaacgg actgaaagc ctattcatt cctggagcg ccaacgacg caagcgcct aaggatctg ccgagggcg accggtgcc atgtcgagg gaggaggtg tcctatctt gggtcagct cgcagagag gaggtccgc cgtcaagcc gaagagaaa gaacgactt cgtgccaat ccgctcctc tacctagtc agtccgcaa gtaaaggat tggctctcg cgccaacag ctggtgatt gtagtgctg ttcatcaat atatcgctg gccatcatg ttcttcaag ctgctgaca tag
- >TC033225 RC
atgcaacag gcgaaccag gtgggggac aacgggggc tcctccccc gcggccacc ggggccacg ggcacggcc accacccgc agccacgtg aacggcggc cgcttcgac ttcgacgac ggcggcacc tactgcggc ggctgggag gacggcaag gcccacgga cacggcgtc tgcaccggc cccaagggc cagggggcc tactccggc tcctggcac tacggcttc gaagtctcc ggggtctat acttggccg agtgggagt tgcttcgaa gggcaatgg caaaatgga aaaagacac ggcttcggg gtggaaacc cgaggccgg tggatttat cggggagag tggacgcag ggctttaaa ggacgatat ggagtgcgg cagtccaac acctccaca gccaaatac gaaggaact tgggcgaat ggcctacaa gatggttac ggatccgag acttacgca gacgatggc acttaccaa ggacaatgg ctgagaggc atgagacat ggctacggc gtaaggaac agtgcgcca ttcggcaaa gcgtccaaa tacagagct aacaagaca ttaagggcg tctctaacg tcgctacgc agtaatgaa ggcaatacg ggagcaaat ccagaagcg gcggaaaaa agagaccgg cgagttgat gattccaga ggaggcttc gtccttaaa atgaactcg gccgaaccc acgaaaagg agacaatcc ctcggctcc ggaaatatt aagaaagga ttgcttatg gggttgaag ttgaagaag caacgaagc actggcgat ttagaaaaa aggggtggt ggaggatct ggcagtatc agatcgaca ggatcatca gcctcttgg gtcagcact gattcggca cagtcaggc atgaccaac gcttctata ccgtccgat tccaatgcg agcttcgtt gttgaggac gagcaaatg gatcctaaa gtaaccgaa acttacatg ggtgagtgg aagaacgac aaaagatca ggcttcggc attagcgag agatccgat ggtctcaaa tacgaaggc gaatggtat gcgaacaag aaatacggt tacggtgta accacgttc agtaacggg gagaaagag gaaggcaaa tacaagaac aacgtcctt ataacgagc caaaagaag aaactgttt ttaatgaga tcagctaaa tttagggaa agaatcgac gccgcagtg aacgccgct caaagagca tcgaaaata gcgttacag aaagcagat attgcgata tcgagaacg gcaacggcc agaggcaaa gccgagcaa gcggaccta gcggctgct caggcgagg gaagactcg gacgtggcc acaactgtt gccaaacaa tacgcgccc gacttcaag cagccgggt ctggaaagg ctgaaagcg cgacaaaac aagtaccaa atcaacaca gacacagtc accctcaac cagaaggag aacccacac aaccaaatt ccaaaccaa gtaccgaac caaattccc aaccaaatt cccaatcaa acgccaaac caagtcaac aaaccactc gcgaaccaa ctcaaccag ataccgaac caaataccc aacaaacca cacaaccaa accaactac ccgccccag caaaccgta ggtttcaac ctccaacaa aaccaaaac gcttataac aacactaac aatgtgtcc aacgagttc ggtccgcag caaaacttc tcaccggac aataaaatc accaacgaa ttccccgga aacaaaccc cggaattcc ctcccagcc gacgacatc tccccgcaa gtgagctcg ctccggagg ggctcgagg cgggtttcc ggcgatagg ccgtactta gggaactta ggtcaacag tcttcgata gatcacttc gatcactac aaaaggccg cccagtcgg gactcgagt gtggatagg tactcccga gccacaggc aggatgagc agcacagtc gggtcccga cagccctca gtggaacgg tcgtctcgt ccttcggag acccccgac aggtccttg cgggcgccg tcggtcgca agggggacg actccggtt ccgacgatt cccaacacg aagaacggg tccgtgttg accggaagt gcggcggga agcaacgcc aagttggct gggaataat ccaggacag ccgccgccg ttcgaagaa atcatcatt cggaagcgg ggactgggc caggatatc attccttct cctaaccaa ccgaaacgg actgaaagc ctattcatt cctggagcg ccaacgacg caagcgcct aagcagtgc tcgcgacgt ttgtggcga acctcgtgc accatgttg tggtaccaa gacgatgac gaggaggag aacctcatg ttttccccc gccctcatg gcacgcaga gcctccgaa agctggatt gatacacca cctgtagag aatctggtg acgcagact tcgttgcaa cgcaagaaa tcactgccc gattttcag gatctgccg agggcgacc ggtgccatg tcgagggag gaggtgtcc tatcttggg tcagctcgc agagaggag gtccgccgt caagccgaa gagaaagaa cgacttcgt gccaatccg ctcctctac ctagtcagt ccgcaagta aaggattgg ctctcgcgc caacagctg gtgattgta gtgctgttc atcaatata tcgctggcc atcatgttc ttcaagctg ctgacatag
- >TC033225 RA
gccgcgccg actaccaca cgaccgatc gaaatgccg agctgatat ttattaata tccgaagcc aagagcgag ctctagcgt gcaagacgc acaacgaca ttgtgagtg gtgatgacg agggtgagt gacggtgcg tggtgagag catgcaaca ggcgaacca ggtggggga caacggggg ctcctcccc cgcggccac cggggccac gggcacggc caccacccg cagccacgt gaacggcgg ccgcttcga cttcgacga cggcggcac ctactgcgg cggctggga ggacggcaa ggcccacgg acacggcgt ctgcaccgg ccccaaggg ccagggggc ctactccgg ctcctggca ctacggctt cgaagtctc cggggtcta tacttggcc gagtgggag ttgcttcga agggcaatg gcaaaatgg aaaaagaca cggcttcgg ggtggaaac ccgaggccg gtggattta tcggggaga gtggacgca gggctttaa aggacgata tggagtgcg gcagtccaa cacctccac agccaaata cgaaggaac ttgggcgaa tggcctaca agatggtta cggatccga gacttacgc agacgatgg cacttacca aggacaatg gctgagagg catgagaca tggctacgg cgtaaggaa cagtgcgcc attcggcaa agcgtccaa atacagagc taacaagac attaagggc gtctctaac gtcgctacg cagtaatga aggcaatac gggagcaaa tccagaagc ggcggaaaa aagagaccg gcgagttga tgattccag aggaggctt cgtccttaa aatgaactc ggccgaacc cacgaaaag gagacaatc cctcggctc cggaaatat taagaaagg attgcttat ggggttgaa gttgaagaa gcaacgaag cactggcga tttagaaaa aaggggtgg tggaggatc tggcagtat cagatcgac aggatcatc agcctcttg ggtcagcac tgattcggc acagtcagg catgaccaa cgcttctat accgtccga ttccaatgc gagcttcgt tgttgagga cgagcaaat ggatcctaa agtaaccga aacttacat gggtgagtg gaagaacga caaaagatc aggcttcgg cattagcga gagatccga tggtctcaa atacgaagg cgaatggta tgcgaacaa gaaatacgg ttacggtgt aaccacgtt cagtaacgg ggagaaaga ggaaggcaa atacaagaa caacgtcct tataacgag ccaaaagaa gaaactgtt tttaatgag atcagctaa atttaggga aagaatcga cgccgcagt gaacgccgc tcaaagagc atcgaaaat agcgttaca gaaagcaga tattgcgat atcgagaac ggcaacggc cagaggcaa agccgagca agcggacct agcggctgc tcaggcgag ggaagactc ggacgtggc cacaactgt tgccaaaca atacgcgcc cgacttcaa gcagccggg tctggaaag gctgaaagc gcgacaaaa caagtacca aatcaacac agacacagt caccctcaa ccagaagga gaacccaca caaccaaat tccaaacca agtaccgaa ccaaattcc caaccaaat tcccaatca aacgccaaa ccaagtcaa caaaccact cgcgaacca actcaacca gataccgaa ccaaatacc caacaaacc acacaacca aaccaacta cccgcccca gcaaaccgt aggtttcaa cctccaaca aaaccaaaa cgcttataa caacactaa caatgtgtc caacgagtt cggtccgca gcaaaactt ctcaccgga caataaaat caccaacga attccccgg aaacaaacc ccggaattc cctcccagc cgacgacat ctccccgca agtgagctc gctccggag gggctcgag gcgggtttc cggcgatag gccgtactt agggaactt aggtcaaca gtcttcgat agatcactt cgatcacta caaaaggcc gcccagtcg ggactcgag tgtggatag gtactcccg agccacagg caggatgag cagcacagt cgggtcccg acagccctc agtggaacg gtcgtctcg tccttcgga gacccccga caggtcctt gcgggcgcc gtcggtcgc aagggggac gactccggt tccgacgat tcccaacac gaagaacgg gtccgtgtt gaccggaag tgcggcggg aagcaacgc caagttggc tgggaataa tccaggaca gccgccgcc gttcgaaga aatcatcat tcggaagcg gggactggg ccaggatat cattccttc tcctaacca accgaaacg gactgaaag cctattcat tcctggagc gccaacgac gcaagcgcc taagaatct ggtgacgca gacttcgtt gcaacgcaa gaaatcact gcccgattt tcaggatct gccgagggc gaccggtgc catgtcgag ggaggaggt gtcctatct tgggtcagc tcgcagaga ggaggtccg ccgtcaagc cgaagagaa agaacgact tcgtgccaa tccgctcct ctacctagt cagtccgca agtaaagga ttggctctc gcgccaaca gctggtgat tgtagtgct gttcatcaa tatatcgct ggccatcat gttcttcaa gctgctgac atagtacag gctgggcgt caaaggacg cgtgtttca gccgattac ccaccaact agacaattg atgaggatt gtatattag gacttattt atataacac agatgggtc atgcctgac taagtgtcg actattata ttctaacgc gtgacttgt tgggaagct gtgtttggg aacattttc gctgttgga gtttttctc agattttat ctacacttg tttgtttat ttcatcttg ttgttggcc accccagct cttcggaaa tttgttatg tttacttct ctcccattt accaaatgg cggtgtaaa taattgtta cgttaggtt ttagactaa atattttat atcgcgttt agtggggca aacacagac gaggtgaag caaaccaac tgaataatt tatcattat atttgactt gttgtaagc gtttagagg caaagtata tttagttgt agcttatac ctatatcgt tctcggact gagcccact gtatcagcg gaacggcgc aaactggcg tcttggggc tccatcatc cttggccag gcctcggtg cccgatcgt ttcgtttaa tcaaaattt cacggacac tgacgcctg ctcacgatg acgcactaa tacttaatt tttgtaact ctcttgcag gcaggagac attggttgt agatatgga aacggaagt gtttgttta aattcaaat tttaaatgt ttagctctt ttacatatg aatttataa aacgttttc taaactatt tttgcgtgc cttgttcaa cttaagtga ttcgtacat actatttgt aattatgca gacatttgt atgagcatt aaacaaaat ttctagatt tatatggaa tcgaatgtg taacgatgt gtctatcac tttatattt tcaattaaa taaacaaaa agggctgac
- >TC033225 RB
gccgcgccg actaccaca cgaccgatc gaaatgccg agctgatat ttattaata tccgaagcc aagagcgag ctctagcgt gcaagacgc acaacgaca ttgtgagtg gtgatgacg agggtgagt gacggtgcg tggtgagag catgcaaca ggcgaacca ggtggggga caacggggg ctcctcccc cgcggccac cggggccac gggcacggc caccacccg cagccacgt gaacggcgg ccgcttcga cttcgacga cggcggcac ctactgcgg cggctggga ggacggcaa ggcccacgg acacggcgt ctgcaccgg ccccaaggg ccagggggc ctactccgg ctcctggca ctacggctt cgaagtctc cggggtcta tacttggcc gagtgggag ttgcttcga agggcaatg gcaaaatgg aaaaagaca cggcttcgg ggtggaaac ccgaggccg gtggattta tcggggaga gtggacgca gggctttaa aggacgata tggagtgcg gcagtccaa cacctccac agccaaata cgaaggaac ttgggcgaa tggcctaca agatggtta cggatccga gacttacgc agacgatgg cacttacca aggacaatg gctgagagg catgagaca tggctacgg cgtaaggaa cagtgcgcc attcggcaa agcgtccaa atacagagc taacaagac attaagggc gtctctaac gtcgctacg cagtaatga aggcaatac gggagcaaa tccagaagc ggcggaaaa aagagaccg gcgagttga tgattccag aggaggctt cgtccttaa aatgaactc ggccgaacc cacgaaaag gagacaatc cctcggctc cggaaatat taagaaagg attgcttat ggggttgaa gttgaagaa gcaacgaag cactggcga tttagaaaa aaggggtgg tggaggatc tggcagtat cagatcgac aggatcatc agcctcttg ggtcagcac tgattcggc acagtcagg catgaccaa cgcttctat accgtccga ttccaatgc gagcttcgt tgttgagga cgagcaaat ggatcctaa agtaaccga aacttacat gggtgagtg gaagaacga caaaagatc aggcttcgg cattagcga gagatccga tggtctcaa atacgaagg cgaatggta tgcgaacaa gaaatacgg ttacggtgt aaccacgtt cagtaacgg ggagaaaga ggaaggcaa atacaagaa caacgtcct tataacgag ccaaaagaa gaaactgtt tttaatgag atcagctaa atttaggga aagaatcga cgccgcagt gaacgccgc tcaaagagc atcgaaaat agcgttaca gaaagcaga tattgcgat atcgagaac ggcaacggc cagaggcaa agccgagca agcggacct agcggctgc tcaggcgag ggaagactc ggacgtggc cacaactgt tgccaaaca atacgcgcc cgacttcaa gcagccggg tctggaaag gctgaaagc gcgacaaaa caagtacca aatcaacac agacacagt caccctcaa ccagaagga gaacccaca caaccaaat tccaaacca agtaccgaa ccaaattcc caaccaaat tcccaatca aacgccaaa ccaagtcaa caaaccact cgcgaacca actcaacca gataccgaa ccaaatacc caacaaacc acacaacca aaccaacta cccgcccca gcaaaccgt aggtttcaa cctccaaca aaaccaaaa cgcttataa caacactaa caatgtgtc caacgagtt cggtccgca gcaaaactt ctcaccgga caataaaat caccaacga attccccgg aaacaaacc ccggaattc cctcccagc cgacgacat ctccccgca agtgagctc gctccggag gggctcgag gcgggtttc cggcgatag gccgtactt agggaactt aggtcaaca gtcttcgat agatcactt cgatcacta caaaaggcc gcccagtcg ggactcgag tgtggatag gtactcccg agccacagg caggatgag cagcacagt cgggtcccg acagccctc agtggaacg gtcgtctcg tccttcgga gacccccga caggtcctt gcgggcgcc gtcggtcgc aagggggac gactccggt tccgacgat tcccaacac gaagaacgg gtccgtgtt gaccggaag tgcggcggg aagcaacgc caagttggc tgggaataa tccaggaca gccgccgcc gttcgaaga aatcatcat tcggaagcg gggactggg ccaggatat cattccttc tcctaacca accgaaacg gactgaaag cctattcat tcctggagc gccaacgac gcaagcgcc taaggatct gccgagggc gaccggtgc catgtcgag ggaggaggt gtcctatct tgggtcagc tcgcagaga ggaggtccg ccgtcaagc cgaagagaa agaacgact tcgtgccaa tccgctcct ctacctagt cagtccgca agtaaagga ttggctctc gcgccaaca gctggtgat tgtagtgct gttcatcaa tatatcgct ggccatcat gttcttcaa gctgctgac atagtacag gctgggcgt caaaggacg cgtgtttca gccgattac ccaccaact agacaattg atgaggatt gtatattag gacttattt atataacac agatgggtc atgcctgac taagtgtcg actattata ttctaacgc gtgacttgt tgggaagct gtgtttggg aacattttc gctgttgga gtttttctc agattttat ctacacttg tttgtttat ttcatcttg ttgttggcc accccagct cttcggaaa tttgttatg tttacttct ctcccattt accaaatgg cggtgtaaa taattgtta cgttaggtt ttagactaa atattttat atcgcgttt agtggggca aacacagac gaggtgaag caaaccaac tgaataatt tatcattat atttgactt gttgtaagc gtttagagg caaagtata tttagttgt agcttatac ctatatcgt tctcggact gagcccact gtatcagcg gaacggcgc aaactggcg tcttggggc tccatcatc cttggccag gcctcggtg cccgatcgt ttcgtttaa tcaaaattt cacggacac tgacgcctg ctcacgatg acgcactaa tacttaatt tttgtaact ctcttgcag gcaggagac attggttgt agatatgga aacggaagt gtttgttta aattcaaat tttaaatgt ttagctctt ttacatatg aatttataa aacgttttc taaactatt tttgcgtgc cttgttcaa cttaagtga ttcgtacat actatttgt aattatgca gacatttgt atgagcatt aaacaaaat ttctagatt tatatggaa tcgaatgtg taacgatgt gtctatcac tttatattt tcaattaaa taaacaaaa agggctgac
- >TC033225 RC
gccgcgccg actaccaca cgaccgatc gaaatgccg agctgatat ttattaata tccgaagcc aagagcgag ctctagcgt gcaagacgc acaacgaca ttgtgagtg gtgatgacg agggtgagt gacggtgcg tggtgagag catgcaaca ggcgaacca ggtggggga caacggggg ctcctcccc cgcggccac cggggccac gggcacggc caccacccg cagccacgt gaacggcgg ccgcttcga cttcgacga cggcggcac ctactgcgg cggctggga ggacggcaa ggcccacgg acacggcgt ctgcaccgg ccccaaggg ccagggggc ctactccgg ctcctggca ctacggctt cgaagtctc cggggtcta tacttggcc gagtgggag ttgcttcga agggcaatg gcaaaatgg aaaaagaca cggcttcgg ggtggaaac ccgaggccg gtggattta tcggggaga gtggacgca gggctttaa aggacgata tggagtgcg gcagtccaa cacctccac agccaaata cgaaggaac ttgggcgaa tggcctaca agatggtta cggatccga gacttacgc agacgatgg cacttacca aggacaatg gctgagagg catgagaca tggctacgg cgtaaggaa cagtgcgcc attcggcaa agcgtccaa atacagagc taacaagac attaagggc gtctctaac gtcgctacg cagtaatga aggcaatac gggagcaaa tccagaagc ggcggaaaa aagagaccg gcgagttga tgattccag aggaggctt cgtccttaa aatgaactc ggccgaacc cacgaaaag gagacaatc cctcggctc cggaaatat taagaaagg attgcttat ggggttgaa gttgaagaa gcaacgaag cactggcga tttagaaaa aaggggtgg tggaggatc tggcagtat cagatcgac aggatcatc agcctcttg ggtcagcac tgattcggc acagtcagg catgaccaa cgcttctat accgtccga ttccaatgc gagcttcgt tgttgagga cgagcaaat ggatcctaa agtaaccga aacttacat gggtgagtg gaagaacga caaaagatc aggcttcgg cattagcga gagatccga tggtctcaa atacgaagg cgaatggta tgcgaacaa gaaatacgg ttacggtgt aaccacgtt cagtaacgg ggagaaaga ggaaggcaa atacaagaa caacgtcct tataacgag ccaaaagaa gaaactgtt tttaatgag atcagctaa atttaggga aagaatcga cgccgcagt gaacgccgc tcaaagagc atcgaaaat agcgttaca gaaagcaga tattgcgat atcgagaac ggcaacggc cagaggcaa agccgagca agcggacct agcggctgc tcaggcgag ggaagactc ggacgtggc cacaactgt tgccaaaca atacgcgcc cgacttcaa gcagccggg tctggaaag gctgaaagc gcgacaaaa caagtacca aatcaacac agacacagt caccctcaa ccagaagga gaacccaca caaccaaat tccaaacca agtaccgaa ccaaattcc caaccaaat tcccaatca aacgccaaa ccaagtcaa caaaccact cgcgaacca actcaacca gataccgaa ccaaatacc caacaaacc acacaacca aaccaacta cccgcccca gcaaaccgt aggtttcaa cctccaaca aaaccaaaa cgcttataa caacactaa caatgtgtc caacgagtt cggtccgca gcaaaactt ctcaccgga caataaaat caccaacga attccccgg aaacaaacc ccggaattc cctcccagc cgacgacat ctccccgca agtgagctc gctccggag gggctcgag gcgggtttc cggcgatag gccgtactt agggaactt aggtcaaca gtcttcgat agatcactt cgatcacta caaaaggcc gcccagtcg ggactcgag tgtggatag gtactcccg agccacagg caggatgag cagcacagt cgggtcccg acagccctc agtggaacg gtcgtctcg tccttcgga gacccccga caggtcctt gcgggcgcc gtcggtcgc aagggggac gactccggt tccgacgat tcccaacac gaagaacgg gtccgtgtt gaccggaag tgcggcggg aagcaacgc caagttggc tgggaataa tccaggaca gccgccgcc gttcgaaga aatcatcat tcggaagcg gggactggg ccaggatat cattccttc tcctaacca accgaaacg gactgaaag cctattcat tcctggagc gccaacgac gcaagcgcc taagcagtg ctcgcgacg tttgtggcg aacctcgtg caccatgtt gtggtacca agacgatga cgaggagga gaacctcat gttttcccc cgccctcat ggcacgcag agcctccga aagctggat tgatacacc acctgtaga gaatctggt gacgcagac ttcgttgca acgcaagaa atcactgcc cgattttca ggatctgcc gagggcgac cggtgccat gtcgaggga ggaggtgtc ctatcttgg gtcagctcg cagagagga ggtccgccg tcaagccga agagaaaga acgacttcg tgccaatcc gctcctcta cctagtcag tccgcaagt aaaggattg gctctcgcg ccaacagct ggtgattgt agtgctgtt catcaatat atcgctggc catcatgtt cttcaagct gctgacata gtacaggct gggcgtcaa aggacgcgt gtttcagcc gattaccca ccaactaga caattgatg aggattgta tattaggac ttatttata taacacaga tgggtcatg cctgactaa gtgtcgact attatattc taacgcgtg acttgttgg gaagctgtg tttgggaac attttcgct gttggagtt tttctcaga ttttatcta cacttgttt gtttatttc atcttgttg ttggccacc ccagctctt cggaaattt gttatgttt acttctctc ccatttacc aaatggcgg tgtaaataa ttgttacgt taggtttta gactaaata ttttatatc gcgtttagt ggggcaaac acagacgag gtgaagcaa accaactga ataatttat cattatatt tgacttgtt gtaagcgtt tagaggcaa agtatattt agttgtagc ttataccta tatcgttct cggactgag cccactgta tcagcggaa cggcgcaaa ctggcgtct tggggctcc atcatcctt ggccaggcc tcggtgccc gatcgtttc gtttaatca aaatttcac ggacactga cgcctgctc acgatgacg cactaatac ttaattttt gtaactctc ttgcaggca ggagacatt ggttgtaga tatggaaac ggaagtgtt tgtttaaat tcaaatttt aaatgttta gctctttta catatgaat ttataaaac gttttctaa actattttt gcgtgcctt gttcaactt aagtgattc gtacatact atttgtaat tatgcagac atttgtatg agcattaaa caaaatttc tagatttat atggaatcg aatgtgtaa cgatgtgtc tatcacttt atattttca attaaataa acaaaaagg gctgac
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