Status
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Gene information
General information
TC033225
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014682,TC014684,TC014681,TC014683
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13394366..13416135
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033225
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05673

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    Pupal morphology
    antenna unilateral orientation irregular - Number of animals showing the phenotype: 2
    wings shortened - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033225 RA
      MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD 
      FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH 
      YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR 
      WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ 
      DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK 
      ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR 
      RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM 
      GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST 
      DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM 
      GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV 
      TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE 
      RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ 
      ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA 
      RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI 
      PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ 
      QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI 
      TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR 
      PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG 
      RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT 
      TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP 
      FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT 
      QAPKNLVTQ TSLQRKKSL PDFQDLPRA TGAMSREEV 
      SYLGSARRE EVRRQAEEK ERLRANPLL YLVSPQVKD 
      WLSRQQLVI VVLFINISL AIMFFKLLT
    • >TC033225 RB
      MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD 
      FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH 
      YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR 
      WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ 
      DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK 
      ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR 
      RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM 
      GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST 
      DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM 
      GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV 
      TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE 
      RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ 
      ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA 
      RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI 
      PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ 
      QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI 
      TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR 
      PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG 
      RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT 
      TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP 
      FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT 
      QAPKDLPRA TGAMSREEV SYLGSARRE EVRRQAEEK 
      ERLRANPLL YLVSPQVKD WLSRQQLVI VVLFINISL 
      AIMFFKLLT
    • >TC033225 RC
      MQQANQVGD NGGSSPAAT GATGTATTR SHVNGGRFD 
      FDDGGTYCG GWEDGKAHG HGVCTGPKG QGAYSGSWH 
      YGFEVSGVY TWPSGSCFE GQWQNGKRH GFGVETRGR 
      WIYRGEWTQ GFKGRYGVR QSNTSTAKY EGTWANGLQ 
      DGYGSETYA DDGTYQGQW LRGMRHGYG VRNSAPFGK 
      ASKYRANKT LRASLTSLR SNEGNTGAN PEAAEKRDR 
      RVDDSRGGF VLKMNSAEP TKRRQSLGS GNIKKGLLM 
      GLKLKKQRS TGDLEKRGG GGSGSIRST GSSASWVST 
      DSAQSGMTN ASIPSDSNA SFVVEDEQM DPKVTETYM 
      GEWKNDKRS GFGISERSD GLKYEGEWY ANKKYGYGV 
      TTFSNGEKE EGKYKNNVL ITSQKKKLF LMRSAKFRE 
      RIDAAVNAA QRASKIALQ KADIAISRT ATARGKAEQ 
      ADLAAAQAR EDSDVATTV AKQYAPDFK QPGLERLKA 
      RQNKYQINT DTVTLNQKE NPHNQIPNQ VPNQIPNQI 
      PNQTPNQVN KPLANQLNQ IPNQIPNKP HNQTNYPPQ 
      QTVGFNLQQ NQNAYNNTN NVSNEFGPQ QNFSPDNKI 
      TNEFPGNKP RNSLPADDI SPQVSSLRR GSRRVSGDR 
      PYLGNLGQQ SSIDHFDHY KRPPSRDSS VDRYSRATG 
      RMSSTVGSR QPSVERSSR PSETPDRSL RAPSVARGT 
      TPVPTIPNT KNGSVLTGS AAGSNAKLA GNNPGQPPP 
      FEEIIIRKR GLGQDIIPS PNQPKRTES LFIPGAPTT 
      QAPKQCSRR LWRTSCTML WYQDDDEEE NLMFSPALM 
      ARRASESWI DTPPVENLV TQTSLQRKK SLPDFQDLP 
      RATGAMSRE EVSYLGSAR REEVRRQAE EKERLRANP 
      LLYLVSPQV KDWLSRQQL VIVVLFINI SLAIMFFKL 
      LT
    CDS sequence
    • >TC033225 RA
      atgcaacag gcgaaccag gtgggggac aacgggggc 
      tcctccccc gcggccacc ggggccacg ggcacggcc 
      accacccgc agccacgtg aacggcggc cgcttcgac 
      ttcgacgac ggcggcacc tactgcggc ggctgggag 
      gacggcaag gcccacgga cacggcgtc tgcaccggc 
      cccaagggc cagggggcc tactccggc tcctggcac 
      tacggcttc gaagtctcc ggggtctat acttggccg 
      agtgggagt tgcttcgaa gggcaatgg caaaatgga 
      aaaagacac ggcttcggg gtggaaacc cgaggccgg 
      tggatttat cggggagag tggacgcag ggctttaaa 
      ggacgatat ggagtgcgg cagtccaac acctccaca 
      gccaaatac gaaggaact tgggcgaat ggcctacaa 
      gatggttac ggatccgag acttacgca gacgatggc 
      acttaccaa ggacaatgg ctgagaggc atgagacat 
      ggctacggc gtaaggaac agtgcgcca ttcggcaaa 
      gcgtccaaa tacagagct aacaagaca ttaagggcg 
      tctctaacg tcgctacgc agtaatgaa ggcaatacg 
      ggagcaaat ccagaagcg gcggaaaaa agagaccgg 
      cgagttgat gattccaga ggaggcttc gtccttaaa 
      atgaactcg gccgaaccc acgaaaagg agacaatcc 
      ctcggctcc ggaaatatt aagaaagga ttgcttatg 
      gggttgaag ttgaagaag caacgaagc actggcgat 
      ttagaaaaa aggggtggt ggaggatct ggcagtatc 
      agatcgaca ggatcatca gcctcttgg gtcagcact 
      gattcggca cagtcaggc atgaccaac gcttctata 
      ccgtccgat tccaatgcg agcttcgtt gttgaggac 
      gagcaaatg gatcctaaa gtaaccgaa acttacatg 
      ggtgagtgg aagaacgac aaaagatca ggcttcggc 
      attagcgag agatccgat ggtctcaaa tacgaaggc 
      gaatggtat gcgaacaag aaatacggt tacggtgta 
      accacgttc agtaacggg gagaaagag gaaggcaaa 
      tacaagaac aacgtcctt ataacgagc caaaagaag 
      aaactgttt ttaatgaga tcagctaaa tttagggaa 
      agaatcgac gccgcagtg aacgccgct caaagagca 
      tcgaaaata gcgttacag aaagcagat attgcgata 
      tcgagaacg gcaacggcc agaggcaaa gccgagcaa 
      gcggaccta gcggctgct caggcgagg gaagactcg 
      gacgtggcc acaactgtt gccaaacaa tacgcgccc 
      gacttcaag cagccgggt ctggaaagg ctgaaagcg 
      cgacaaaac aagtaccaa atcaacaca gacacagtc 
      accctcaac cagaaggag aacccacac aaccaaatt 
      ccaaaccaa gtaccgaac caaattccc aaccaaatt 
      cccaatcaa acgccaaac caagtcaac aaaccactc 
      gcgaaccaa ctcaaccag ataccgaac caaataccc 
      aacaaacca cacaaccaa accaactac ccgccccag 
      caaaccgta ggtttcaac ctccaacaa aaccaaaac 
      gcttataac aacactaac aatgtgtcc aacgagttc 
      ggtccgcag caaaacttc tcaccggac aataaaatc 
      accaacgaa ttccccgga aacaaaccc cggaattcc 
      ctcccagcc gacgacatc tccccgcaa gtgagctcg 
      ctccggagg ggctcgagg cgggtttcc ggcgatagg 
      ccgtactta gggaactta ggtcaacag tcttcgata 
      gatcacttc gatcactac aaaaggccg cccagtcgg 
      gactcgagt gtggatagg tactcccga gccacaggc 
      aggatgagc agcacagtc gggtcccga cagccctca 
      gtggaacgg tcgtctcgt ccttcggag acccccgac 
      aggtccttg cgggcgccg tcggtcgca agggggacg 
      actccggtt ccgacgatt cccaacacg aagaacggg 
      tccgtgttg accggaagt gcggcggga agcaacgcc 
      aagttggct gggaataat ccaggacag ccgccgccg 
      ttcgaagaa atcatcatt cggaagcgg ggactgggc 
      caggatatc attccttct cctaaccaa ccgaaacgg 
      actgaaagc ctattcatt cctggagcg ccaacgacg 
      caagcgcct aagaatctg gtgacgcag acttcgttg 
      caacgcaag aaatcactg cccgatttt caggatctg 
      ccgagggcg accggtgcc atgtcgagg gaggaggtg 
      tcctatctt gggtcagct cgcagagag gaggtccgc 
      cgtcaagcc gaagagaaa gaacgactt cgtgccaat 
      ccgctcctc tacctagtc agtccgcaa gtaaaggat 
      tggctctcg cgccaacag ctggtgatt gtagtgctg 
      ttcatcaat atatcgctg gccatcatg ttcttcaag 
      ctgctgaca tag
    • >TC033225 RB
      atgcaacag gcgaaccag gtgggggac aacgggggc 
      tcctccccc gcggccacc ggggccacg ggcacggcc 
      accacccgc agccacgtg aacggcggc cgcttcgac 
      ttcgacgac ggcggcacc tactgcggc ggctgggag 
      gacggcaag gcccacgga cacggcgtc tgcaccggc 
      cccaagggc cagggggcc tactccggc tcctggcac 
      tacggcttc gaagtctcc ggggtctat acttggccg 
      agtgggagt tgcttcgaa gggcaatgg caaaatgga 
      aaaagacac ggcttcggg gtggaaacc cgaggccgg 
      tggatttat cggggagag tggacgcag ggctttaaa 
      ggacgatat ggagtgcgg cagtccaac acctccaca 
      gccaaatac gaaggaact tgggcgaat ggcctacaa 
      gatggttac ggatccgag acttacgca gacgatggc 
      acttaccaa ggacaatgg ctgagaggc atgagacat 
      ggctacggc gtaaggaac agtgcgcca ttcggcaaa 
      gcgtccaaa tacagagct aacaagaca ttaagggcg 
      tctctaacg tcgctacgc agtaatgaa ggcaatacg 
      ggagcaaat ccagaagcg gcggaaaaa agagaccgg 
      cgagttgat gattccaga ggaggcttc gtccttaaa 
      atgaactcg gccgaaccc acgaaaagg agacaatcc 
      ctcggctcc ggaaatatt aagaaagga ttgcttatg 
      gggttgaag ttgaagaag caacgaagc actggcgat 
      ttagaaaaa aggggtggt ggaggatct ggcagtatc 
      agatcgaca ggatcatca gcctcttgg gtcagcact 
      gattcggca cagtcaggc atgaccaac gcttctata 
      ccgtccgat tccaatgcg agcttcgtt gttgaggac 
      gagcaaatg gatcctaaa gtaaccgaa acttacatg 
      ggtgagtgg aagaacgac aaaagatca ggcttcggc 
      attagcgag agatccgat ggtctcaaa tacgaaggc 
      gaatggtat gcgaacaag aaatacggt tacggtgta 
      accacgttc agtaacggg gagaaagag gaaggcaaa 
      tacaagaac aacgtcctt ataacgagc caaaagaag 
      aaactgttt ttaatgaga tcagctaaa tttagggaa 
      agaatcgac gccgcagtg aacgccgct caaagagca 
      tcgaaaata gcgttacag aaagcagat attgcgata 
      tcgagaacg gcaacggcc agaggcaaa gccgagcaa 
      gcggaccta gcggctgct caggcgagg gaagactcg 
      gacgtggcc acaactgtt gccaaacaa tacgcgccc 
      gacttcaag cagccgggt ctggaaagg ctgaaagcg 
      cgacaaaac aagtaccaa atcaacaca gacacagtc 
      accctcaac cagaaggag aacccacac aaccaaatt 
      ccaaaccaa gtaccgaac caaattccc aaccaaatt 
      cccaatcaa acgccaaac caagtcaac aaaccactc 
      gcgaaccaa ctcaaccag ataccgaac caaataccc 
      aacaaacca cacaaccaa accaactac ccgccccag 
      caaaccgta ggtttcaac ctccaacaa aaccaaaac 
      gcttataac aacactaac aatgtgtcc aacgagttc 
      ggtccgcag caaaacttc tcaccggac aataaaatc 
      accaacgaa ttccccgga aacaaaccc cggaattcc 
      ctcccagcc gacgacatc tccccgcaa gtgagctcg 
      ctccggagg ggctcgagg cgggtttcc ggcgatagg 
      ccgtactta gggaactta ggtcaacag tcttcgata 
      gatcacttc gatcactac aaaaggccg cccagtcgg 
      gactcgagt gtggatagg tactcccga gccacaggc 
      aggatgagc agcacagtc gggtcccga cagccctca 
      gtggaacgg tcgtctcgt ccttcggag acccccgac 
      aggtccttg cgggcgccg tcggtcgca agggggacg 
      actccggtt ccgacgatt cccaacacg aagaacggg 
      tccgtgttg accggaagt gcggcggga agcaacgcc 
      aagttggct gggaataat ccaggacag ccgccgccg 
      ttcgaagaa atcatcatt cggaagcgg ggactgggc 
      caggatatc attccttct cctaaccaa ccgaaacgg 
      actgaaagc ctattcatt cctggagcg ccaacgacg 
      caagcgcct aaggatctg ccgagggcg accggtgcc 
      atgtcgagg gaggaggtg tcctatctt gggtcagct 
      cgcagagag gaggtccgc cgtcaagcc gaagagaaa 
      gaacgactt cgtgccaat ccgctcctc tacctagtc 
      agtccgcaa gtaaaggat tggctctcg cgccaacag 
      ctggtgatt gtagtgctg ttcatcaat atatcgctg 
      gccatcatg ttcttcaag ctgctgaca tag
    • >TC033225 RC
      atgcaacag gcgaaccag gtgggggac aacgggggc 
      tcctccccc gcggccacc ggggccacg ggcacggcc 
      accacccgc agccacgtg aacggcggc cgcttcgac 
      ttcgacgac ggcggcacc tactgcggc ggctgggag 
      gacggcaag gcccacgga cacggcgtc tgcaccggc 
      cccaagggc cagggggcc tactccggc tcctggcac 
      tacggcttc gaagtctcc ggggtctat acttggccg 
      agtgggagt tgcttcgaa gggcaatgg caaaatgga 
      aaaagacac ggcttcggg gtggaaacc cgaggccgg 
      tggatttat cggggagag tggacgcag ggctttaaa 
      ggacgatat ggagtgcgg cagtccaac acctccaca 
      gccaaatac gaaggaact tgggcgaat ggcctacaa 
      gatggttac ggatccgag acttacgca gacgatggc 
      acttaccaa ggacaatgg ctgagaggc atgagacat 
      ggctacggc gtaaggaac agtgcgcca ttcggcaaa 
      gcgtccaaa tacagagct aacaagaca ttaagggcg 
      tctctaacg tcgctacgc agtaatgaa ggcaatacg 
      ggagcaaat ccagaagcg gcggaaaaa agagaccgg 
      cgagttgat gattccaga ggaggcttc gtccttaaa 
      atgaactcg gccgaaccc acgaaaagg agacaatcc 
      ctcggctcc ggaaatatt aagaaagga ttgcttatg 
      gggttgaag ttgaagaag caacgaagc actggcgat 
      ttagaaaaa aggggtggt ggaggatct ggcagtatc 
      agatcgaca ggatcatca gcctcttgg gtcagcact 
      gattcggca cagtcaggc atgaccaac gcttctata 
      ccgtccgat tccaatgcg agcttcgtt gttgaggac 
      gagcaaatg gatcctaaa gtaaccgaa acttacatg 
      ggtgagtgg aagaacgac aaaagatca ggcttcggc 
      attagcgag agatccgat ggtctcaaa tacgaaggc 
      gaatggtat gcgaacaag aaatacggt tacggtgta 
      accacgttc agtaacggg gagaaagag gaaggcaaa 
      tacaagaac aacgtcctt ataacgagc caaaagaag 
      aaactgttt ttaatgaga tcagctaaa tttagggaa 
      agaatcgac gccgcagtg aacgccgct caaagagca 
      tcgaaaata gcgttacag aaagcagat attgcgata 
      tcgagaacg gcaacggcc agaggcaaa gccgagcaa 
      gcggaccta gcggctgct caggcgagg gaagactcg 
      gacgtggcc acaactgtt gccaaacaa tacgcgccc 
      gacttcaag cagccgggt ctggaaagg ctgaaagcg 
      cgacaaaac aagtaccaa atcaacaca gacacagtc 
      accctcaac cagaaggag aacccacac aaccaaatt 
      ccaaaccaa gtaccgaac caaattccc aaccaaatt 
      cccaatcaa acgccaaac caagtcaac aaaccactc 
      gcgaaccaa ctcaaccag ataccgaac caaataccc 
      aacaaacca cacaaccaa accaactac ccgccccag 
      caaaccgta ggtttcaac ctccaacaa aaccaaaac 
      gcttataac aacactaac aatgtgtcc aacgagttc 
      ggtccgcag caaaacttc tcaccggac aataaaatc 
      accaacgaa ttccccgga aacaaaccc cggaattcc 
      ctcccagcc gacgacatc tccccgcaa gtgagctcg 
      ctccggagg ggctcgagg cgggtttcc ggcgatagg 
      ccgtactta gggaactta ggtcaacag tcttcgata 
      gatcacttc gatcactac aaaaggccg cccagtcgg 
      gactcgagt gtggatagg tactcccga gccacaggc 
      aggatgagc agcacagtc gggtcccga cagccctca 
      gtggaacgg tcgtctcgt ccttcggag acccccgac 
      aggtccttg cgggcgccg tcggtcgca agggggacg 
      actccggtt ccgacgatt cccaacacg aagaacggg 
      tccgtgttg accggaagt gcggcggga agcaacgcc 
      aagttggct gggaataat ccaggacag ccgccgccg 
      ttcgaagaa atcatcatt cggaagcgg ggactgggc 
      caggatatc attccttct cctaaccaa ccgaaacgg 
      actgaaagc ctattcatt cctggagcg ccaacgacg 
      caagcgcct aagcagtgc tcgcgacgt ttgtggcga 
      acctcgtgc accatgttg tggtaccaa gacgatgac 
      gaggaggag aacctcatg ttttccccc gccctcatg 
      gcacgcaga gcctccgaa agctggatt gatacacca 
      cctgtagag aatctggtg acgcagact tcgttgcaa 
      cgcaagaaa tcactgccc gattttcag gatctgccg 
      agggcgacc ggtgccatg tcgagggag gaggtgtcc 
      tatcttggg tcagctcgc agagaggag gtccgccgt 
      caagccgaa gagaaagaa cgacttcgt gccaatccg 
      ctcctctac ctagtcagt ccgcaagta aaggattgg 
      ctctcgcgc caacagctg gtgattgta gtgctgttc 
      atcaatata tcgctggcc atcatgttc ttcaagctg 
      ctgacatag
    mRNA sequence
    • >TC033225 RA
      gccgcgccg actaccaca cgaccgatc gaaatgccg 
      agctgatat ttattaata tccgaagcc aagagcgag 
      ctctagcgt gcaagacgc acaacgaca ttgtgagtg 
      gtgatgacg agggtgagt gacggtgcg tggtgagag 
      catgcaaca ggcgaacca ggtggggga caacggggg 
      ctcctcccc cgcggccac cggggccac gggcacggc 
      caccacccg cagccacgt gaacggcgg ccgcttcga 
      cttcgacga cggcggcac ctactgcgg cggctggga 
      ggacggcaa ggcccacgg acacggcgt ctgcaccgg 
      ccccaaggg ccagggggc ctactccgg ctcctggca 
      ctacggctt cgaagtctc cggggtcta tacttggcc 
      gagtgggag ttgcttcga agggcaatg gcaaaatgg 
      aaaaagaca cggcttcgg ggtggaaac ccgaggccg 
      gtggattta tcggggaga gtggacgca gggctttaa 
      aggacgata tggagtgcg gcagtccaa cacctccac 
      agccaaata cgaaggaac ttgggcgaa tggcctaca 
      agatggtta cggatccga gacttacgc agacgatgg 
      cacttacca aggacaatg gctgagagg catgagaca 
      tggctacgg cgtaaggaa cagtgcgcc attcggcaa 
      agcgtccaa atacagagc taacaagac attaagggc 
      gtctctaac gtcgctacg cagtaatga aggcaatac 
      gggagcaaa tccagaagc ggcggaaaa aagagaccg 
      gcgagttga tgattccag aggaggctt cgtccttaa 
      aatgaactc ggccgaacc cacgaaaag gagacaatc 
      cctcggctc cggaaatat taagaaagg attgcttat 
      ggggttgaa gttgaagaa gcaacgaag cactggcga 
      tttagaaaa aaggggtgg tggaggatc tggcagtat 
      cagatcgac aggatcatc agcctcttg ggtcagcac 
      tgattcggc acagtcagg catgaccaa cgcttctat 
      accgtccga ttccaatgc gagcttcgt tgttgagga 
      cgagcaaat ggatcctaa agtaaccga aacttacat 
      gggtgagtg gaagaacga caaaagatc aggcttcgg 
      cattagcga gagatccga tggtctcaa atacgaagg 
      cgaatggta tgcgaacaa gaaatacgg ttacggtgt 
      aaccacgtt cagtaacgg ggagaaaga ggaaggcaa 
      atacaagaa caacgtcct tataacgag ccaaaagaa 
      gaaactgtt tttaatgag atcagctaa atttaggga 
      aagaatcga cgccgcagt gaacgccgc tcaaagagc 
      atcgaaaat agcgttaca gaaagcaga tattgcgat 
      atcgagaac ggcaacggc cagaggcaa agccgagca 
      agcggacct agcggctgc tcaggcgag ggaagactc 
      ggacgtggc cacaactgt tgccaaaca atacgcgcc 
      cgacttcaa gcagccggg tctggaaag gctgaaagc 
      gcgacaaaa caagtacca aatcaacac agacacagt 
      caccctcaa ccagaagga gaacccaca caaccaaat 
      tccaaacca agtaccgaa ccaaattcc caaccaaat 
      tcccaatca aacgccaaa ccaagtcaa caaaccact 
      cgcgaacca actcaacca gataccgaa ccaaatacc 
      caacaaacc acacaacca aaccaacta cccgcccca 
      gcaaaccgt aggtttcaa cctccaaca aaaccaaaa 
      cgcttataa caacactaa caatgtgtc caacgagtt 
      cggtccgca gcaaaactt ctcaccgga caataaaat 
      caccaacga attccccgg aaacaaacc ccggaattc 
      cctcccagc cgacgacat ctccccgca agtgagctc 
      gctccggag gggctcgag gcgggtttc cggcgatag 
      gccgtactt agggaactt aggtcaaca gtcttcgat 
      agatcactt cgatcacta caaaaggcc gcccagtcg 
      ggactcgag tgtggatag gtactcccg agccacagg 
      caggatgag cagcacagt cgggtcccg acagccctc 
      agtggaacg gtcgtctcg tccttcgga gacccccga 
      caggtcctt gcgggcgcc gtcggtcgc aagggggac 
      gactccggt tccgacgat tcccaacac gaagaacgg 
      gtccgtgtt gaccggaag tgcggcggg aagcaacgc 
      caagttggc tgggaataa tccaggaca gccgccgcc 
      gttcgaaga aatcatcat tcggaagcg gggactggg 
      ccaggatat cattccttc tcctaacca accgaaacg 
      gactgaaag cctattcat tcctggagc gccaacgac 
      gcaagcgcc taagaatct ggtgacgca gacttcgtt 
      gcaacgcaa gaaatcact gcccgattt tcaggatct 
      gccgagggc gaccggtgc catgtcgag ggaggaggt 
      gtcctatct tgggtcagc tcgcagaga ggaggtccg 
      ccgtcaagc cgaagagaa agaacgact tcgtgccaa 
      tccgctcct ctacctagt cagtccgca agtaaagga 
      ttggctctc gcgccaaca gctggtgat tgtagtgct 
      gttcatcaa tatatcgct ggccatcat gttcttcaa 
      gctgctgac atagtacag gctgggcgt caaaggacg 
      cgtgtttca gccgattac ccaccaact agacaattg 
      atgaggatt gtatattag gacttattt atataacac 
      agatgggtc atgcctgac taagtgtcg actattata 
      ttctaacgc gtgacttgt tgggaagct gtgtttggg 
      aacattttc gctgttgga gtttttctc agattttat 
      ctacacttg tttgtttat ttcatcttg ttgttggcc 
      accccagct cttcggaaa tttgttatg tttacttct 
      ctcccattt accaaatgg cggtgtaaa taattgtta 
      cgttaggtt ttagactaa atattttat atcgcgttt 
      agtggggca aacacagac gaggtgaag caaaccaac 
      tgaataatt tatcattat atttgactt gttgtaagc 
      gtttagagg caaagtata tttagttgt agcttatac 
      ctatatcgt tctcggact gagcccact gtatcagcg 
      gaacggcgc aaactggcg tcttggggc tccatcatc 
      cttggccag gcctcggtg cccgatcgt ttcgtttaa 
      tcaaaattt cacggacac tgacgcctg ctcacgatg 
      acgcactaa tacttaatt tttgtaact ctcttgcag 
      gcaggagac attggttgt agatatgga aacggaagt 
      gtttgttta aattcaaat tttaaatgt ttagctctt 
      ttacatatg aatttataa aacgttttc taaactatt 
      tttgcgtgc cttgttcaa cttaagtga ttcgtacat 
      actatttgt aattatgca gacatttgt atgagcatt 
      aaacaaaat ttctagatt tatatggaa tcgaatgtg 
      taacgatgt gtctatcac tttatattt tcaattaaa 
      taaacaaaa agggctgac
    • >TC033225 RB
      gccgcgccg actaccaca cgaccgatc gaaatgccg 
      agctgatat ttattaata tccgaagcc aagagcgag 
      ctctagcgt gcaagacgc acaacgaca ttgtgagtg 
      gtgatgacg agggtgagt gacggtgcg tggtgagag 
      catgcaaca ggcgaacca ggtggggga caacggggg 
      ctcctcccc cgcggccac cggggccac gggcacggc 
      caccacccg cagccacgt gaacggcgg ccgcttcga 
      cttcgacga cggcggcac ctactgcgg cggctggga 
      ggacggcaa ggcccacgg acacggcgt ctgcaccgg 
      ccccaaggg ccagggggc ctactccgg ctcctggca 
      ctacggctt cgaagtctc cggggtcta tacttggcc 
      gagtgggag ttgcttcga agggcaatg gcaaaatgg 
      aaaaagaca cggcttcgg ggtggaaac ccgaggccg 
      gtggattta tcggggaga gtggacgca gggctttaa 
      aggacgata tggagtgcg gcagtccaa cacctccac 
      agccaaata cgaaggaac ttgggcgaa tggcctaca 
      agatggtta cggatccga gacttacgc agacgatgg 
      cacttacca aggacaatg gctgagagg catgagaca 
      tggctacgg cgtaaggaa cagtgcgcc attcggcaa 
      agcgtccaa atacagagc taacaagac attaagggc 
      gtctctaac gtcgctacg cagtaatga aggcaatac 
      gggagcaaa tccagaagc ggcggaaaa aagagaccg 
      gcgagttga tgattccag aggaggctt cgtccttaa 
      aatgaactc ggccgaacc cacgaaaag gagacaatc 
      cctcggctc cggaaatat taagaaagg attgcttat 
      ggggttgaa gttgaagaa gcaacgaag cactggcga 
      tttagaaaa aaggggtgg tggaggatc tggcagtat 
      cagatcgac aggatcatc agcctcttg ggtcagcac 
      tgattcggc acagtcagg catgaccaa cgcttctat 
      accgtccga ttccaatgc gagcttcgt tgttgagga 
      cgagcaaat ggatcctaa agtaaccga aacttacat 
      gggtgagtg gaagaacga caaaagatc aggcttcgg 
      cattagcga gagatccga tggtctcaa atacgaagg 
      cgaatggta tgcgaacaa gaaatacgg ttacggtgt 
      aaccacgtt cagtaacgg ggagaaaga ggaaggcaa 
      atacaagaa caacgtcct tataacgag ccaaaagaa 
      gaaactgtt tttaatgag atcagctaa atttaggga 
      aagaatcga cgccgcagt gaacgccgc tcaaagagc 
      atcgaaaat agcgttaca gaaagcaga tattgcgat 
      atcgagaac ggcaacggc cagaggcaa agccgagca 
      agcggacct agcggctgc tcaggcgag ggaagactc 
      ggacgtggc cacaactgt tgccaaaca atacgcgcc 
      cgacttcaa gcagccggg tctggaaag gctgaaagc 
      gcgacaaaa caagtacca aatcaacac agacacagt 
      caccctcaa ccagaagga gaacccaca caaccaaat 
      tccaaacca agtaccgaa ccaaattcc caaccaaat 
      tcccaatca aacgccaaa ccaagtcaa caaaccact 
      cgcgaacca actcaacca gataccgaa ccaaatacc 
      caacaaacc acacaacca aaccaacta cccgcccca 
      gcaaaccgt aggtttcaa cctccaaca aaaccaaaa 
      cgcttataa caacactaa caatgtgtc caacgagtt 
      cggtccgca gcaaaactt ctcaccgga caataaaat 
      caccaacga attccccgg aaacaaacc ccggaattc 
      cctcccagc cgacgacat ctccccgca agtgagctc 
      gctccggag gggctcgag gcgggtttc cggcgatag 
      gccgtactt agggaactt aggtcaaca gtcttcgat 
      agatcactt cgatcacta caaaaggcc gcccagtcg 
      ggactcgag tgtggatag gtactcccg agccacagg 
      caggatgag cagcacagt cgggtcccg acagccctc 
      agtggaacg gtcgtctcg tccttcgga gacccccga 
      caggtcctt gcgggcgcc gtcggtcgc aagggggac 
      gactccggt tccgacgat tcccaacac gaagaacgg 
      gtccgtgtt gaccggaag tgcggcggg aagcaacgc 
      caagttggc tgggaataa tccaggaca gccgccgcc 
      gttcgaaga aatcatcat tcggaagcg gggactggg 
      ccaggatat cattccttc tcctaacca accgaaacg 
      gactgaaag cctattcat tcctggagc gccaacgac 
      gcaagcgcc taaggatct gccgagggc gaccggtgc 
      catgtcgag ggaggaggt gtcctatct tgggtcagc 
      tcgcagaga ggaggtccg ccgtcaagc cgaagagaa 
      agaacgact tcgtgccaa tccgctcct ctacctagt 
      cagtccgca agtaaagga ttggctctc gcgccaaca 
      gctggtgat tgtagtgct gttcatcaa tatatcgct 
      ggccatcat gttcttcaa gctgctgac atagtacag 
      gctgggcgt caaaggacg cgtgtttca gccgattac 
      ccaccaact agacaattg atgaggatt gtatattag 
      gacttattt atataacac agatgggtc atgcctgac 
      taagtgtcg actattata ttctaacgc gtgacttgt 
      tgggaagct gtgtttggg aacattttc gctgttgga 
      gtttttctc agattttat ctacacttg tttgtttat 
      ttcatcttg ttgttggcc accccagct cttcggaaa 
      tttgttatg tttacttct ctcccattt accaaatgg 
      cggtgtaaa taattgtta cgttaggtt ttagactaa 
      atattttat atcgcgttt agtggggca aacacagac 
      gaggtgaag caaaccaac tgaataatt tatcattat 
      atttgactt gttgtaagc gtttagagg caaagtata 
      tttagttgt agcttatac ctatatcgt tctcggact 
      gagcccact gtatcagcg gaacggcgc aaactggcg 
      tcttggggc tccatcatc cttggccag gcctcggtg 
      cccgatcgt ttcgtttaa tcaaaattt cacggacac 
      tgacgcctg ctcacgatg acgcactaa tacttaatt 
      tttgtaact ctcttgcag gcaggagac attggttgt 
      agatatgga aacggaagt gtttgttta aattcaaat 
      tttaaatgt ttagctctt ttacatatg aatttataa 
      aacgttttc taaactatt tttgcgtgc cttgttcaa 
      cttaagtga ttcgtacat actatttgt aattatgca 
      gacatttgt atgagcatt aaacaaaat ttctagatt 
      tatatggaa tcgaatgtg taacgatgt gtctatcac 
      tttatattt tcaattaaa taaacaaaa agggctgac
    • >TC033225 RC
      gccgcgccg actaccaca cgaccgatc gaaatgccg 
      agctgatat ttattaata tccgaagcc aagagcgag 
      ctctagcgt gcaagacgc acaacgaca ttgtgagtg 
      gtgatgacg agggtgagt gacggtgcg tggtgagag 
      catgcaaca ggcgaacca ggtggggga caacggggg 
      ctcctcccc cgcggccac cggggccac gggcacggc 
      caccacccg cagccacgt gaacggcgg ccgcttcga 
      cttcgacga cggcggcac ctactgcgg cggctggga 
      ggacggcaa ggcccacgg acacggcgt ctgcaccgg 
      ccccaaggg ccagggggc ctactccgg ctcctggca 
      ctacggctt cgaagtctc cggggtcta tacttggcc 
      gagtgggag ttgcttcga agggcaatg gcaaaatgg 
      aaaaagaca cggcttcgg ggtggaaac ccgaggccg 
      gtggattta tcggggaga gtggacgca gggctttaa 
      aggacgata tggagtgcg gcagtccaa cacctccac 
      agccaaata cgaaggaac ttgggcgaa tggcctaca 
      agatggtta cggatccga gacttacgc agacgatgg 
      cacttacca aggacaatg gctgagagg catgagaca 
      tggctacgg cgtaaggaa cagtgcgcc attcggcaa 
      agcgtccaa atacagagc taacaagac attaagggc 
      gtctctaac gtcgctacg cagtaatga aggcaatac 
      gggagcaaa tccagaagc ggcggaaaa aagagaccg 
      gcgagttga tgattccag aggaggctt cgtccttaa 
      aatgaactc ggccgaacc cacgaaaag gagacaatc 
      cctcggctc cggaaatat taagaaagg attgcttat 
      ggggttgaa gttgaagaa gcaacgaag cactggcga 
      tttagaaaa aaggggtgg tggaggatc tggcagtat 
      cagatcgac aggatcatc agcctcttg ggtcagcac 
      tgattcggc acagtcagg catgaccaa cgcttctat 
      accgtccga ttccaatgc gagcttcgt tgttgagga 
      cgagcaaat ggatcctaa agtaaccga aacttacat 
      gggtgagtg gaagaacga caaaagatc aggcttcgg 
      cattagcga gagatccga tggtctcaa atacgaagg 
      cgaatggta tgcgaacaa gaaatacgg ttacggtgt 
      aaccacgtt cagtaacgg ggagaaaga ggaaggcaa 
      atacaagaa caacgtcct tataacgag ccaaaagaa 
      gaaactgtt tttaatgag atcagctaa atttaggga 
      aagaatcga cgccgcagt gaacgccgc tcaaagagc 
      atcgaaaat agcgttaca gaaagcaga tattgcgat 
      atcgagaac ggcaacggc cagaggcaa agccgagca 
      agcggacct agcggctgc tcaggcgag ggaagactc 
      ggacgtggc cacaactgt tgccaaaca atacgcgcc 
      cgacttcaa gcagccggg tctggaaag gctgaaagc 
      gcgacaaaa caagtacca aatcaacac agacacagt 
      caccctcaa ccagaagga gaacccaca caaccaaat 
      tccaaacca agtaccgaa ccaaattcc caaccaaat 
      tcccaatca aacgccaaa ccaagtcaa caaaccact 
      cgcgaacca actcaacca gataccgaa ccaaatacc 
      caacaaacc acacaacca aaccaacta cccgcccca 
      gcaaaccgt aggtttcaa cctccaaca aaaccaaaa 
      cgcttataa caacactaa caatgtgtc caacgagtt 
      cggtccgca gcaaaactt ctcaccgga caataaaat 
      caccaacga attccccgg aaacaaacc ccggaattc 
      cctcccagc cgacgacat ctccccgca agtgagctc 
      gctccggag gggctcgag gcgggtttc cggcgatag 
      gccgtactt agggaactt aggtcaaca gtcttcgat 
      agatcactt cgatcacta caaaaggcc gcccagtcg 
      ggactcgag tgtggatag gtactcccg agccacagg 
      caggatgag cagcacagt cgggtcccg acagccctc 
      agtggaacg gtcgtctcg tccttcgga gacccccga 
      caggtcctt gcgggcgcc gtcggtcgc aagggggac 
      gactccggt tccgacgat tcccaacac gaagaacgg 
      gtccgtgtt gaccggaag tgcggcggg aagcaacgc 
      caagttggc tgggaataa tccaggaca gccgccgcc 
      gttcgaaga aatcatcat tcggaagcg gggactggg 
      ccaggatat cattccttc tcctaacca accgaaacg 
      gactgaaag cctattcat tcctggagc gccaacgac 
      gcaagcgcc taagcagtg ctcgcgacg tttgtggcg 
      aacctcgtg caccatgtt gtggtacca agacgatga 
      cgaggagga gaacctcat gttttcccc cgccctcat 
      ggcacgcag agcctccga aagctggat tgatacacc 
      acctgtaga gaatctggt gacgcagac ttcgttgca 
      acgcaagaa atcactgcc cgattttca ggatctgcc 
      gagggcgac cggtgccat gtcgaggga ggaggtgtc 
      ctatcttgg gtcagctcg cagagagga ggtccgccg 
      tcaagccga agagaaaga acgacttcg tgccaatcc 
      gctcctcta cctagtcag tccgcaagt aaaggattg 
      gctctcgcg ccaacagct ggtgattgt agtgctgtt 
      catcaatat atcgctggc catcatgtt cttcaagct 
      gctgacata gtacaggct gggcgtcaa aggacgcgt 
      gtttcagcc gattaccca ccaactaga caattgatg 
      aggattgta tattaggac ttatttata taacacaga 
      tgggtcatg cctgactaa gtgtcgact attatattc 
      taacgcgtg acttgttgg gaagctgtg tttgggaac 
      attttcgct gttggagtt tttctcaga ttttatcta 
      cacttgttt gtttatttc atcttgttg ttggccacc 
      ccagctctt cggaaattt gttatgttt acttctctc 
      ccatttacc aaatggcgg tgtaaataa ttgttacgt 
      taggtttta gactaaata ttttatatc gcgtttagt 
      ggggcaaac acagacgag gtgaagcaa accaactga 
      ataatttat cattatatt tgacttgtt gtaagcgtt 
      tagaggcaa agtatattt agttgtagc ttataccta 
      tatcgttct cggactgag cccactgta tcagcggaa 
      cggcgcaaa ctggcgtct tggggctcc atcatcctt 
      ggccaggcc tcggtgccc gatcgtttc gtttaatca 
      aaatttcac ggacactga cgcctgctc acgatgacg 
      cactaatac ttaattttt gtaactctc ttgcaggca 
      ggagacatt ggttgtaga tatggaaac ggaagtgtt 
      tgtttaaat tcaaatttt aaatgttta gctctttta 
      catatgaat ttataaaac gttttctaa actattttt 
      gcgtgcctt gttcaactt aagtgattc gtacatact 
      atttgtaat tatgcagac atttgtatg agcattaaa 
      caaaatttc tagatttat atggaatcg aatgtgtaa 
      cgatgtgtc tatcacttt atattttca attaaataa 
      acaaaaagg gctgac
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