| General information | |||
| TC033224
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13387223..13389769 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033224 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05672 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC033224 RA
MSTLRFFGA LSKISRTNI KFKNTAQIR GMASQERKH TKLKVVDNI GVIVLDSPG VKVIFVNKI LILILMKMN GKVLEIGST INIERSDGR VHPAIVSSI NYELDIVGV EWFEAGETK GKEIDFNCV TRLNPHIAA IKPDVQRFS ECSSDVALV EKPKKRQSI AVYNNRVKS SKSRITQDI VKPKNSLKD KYPQTSRSV IEHISNSFE SNVVKKVGK LELERIERR KKQAEFKAE KVELTKRNA DNPHWELHQ MIHDYCKNL NYKPLTIND VIEDRLITV CVRKRPLNK SENAKKEVD VITVPSKNQ IIVHEPKTK VDLTKYLEN HLFKFDYTF NEHCDNEIV YKYTAQPLI RTVFEGGFA TCFAYGQTG SGKTYTMSG DIFRTNAHK GIYAMAATD VFRLVASPK YRHLNLIVT CSFFEIYSR KVLDLLNRK GDLKILEDG KQQVQVVGL TEKVVSSVN EVLELINHG NMARTSGQT SANENSSRS HAVFQIYLR PAKKFNSLH GKFSLIDLA GNERGADTL SSTKTTRME GGEINKSLL ALKECIRAL GRKGAHLPF RLSKLTQVL RDSFIGANS KTCMIAMVS PGVSSCENT LNTLRYADR VKELGGGEL QHLSTINEK NNEIISMGL EEEKSFSDN SEEFKIVKE KVVNLHREI ISKLQDATK EAKYLNKCI DNEPEKYCR EFEEFVDDT VAFLLNVKN SVIEYKMKL LAEE
- >TC033224 RB
MSTLRFFGA LSKISRTNI KFKNTGAFS AQIRGMASQ ERKHTKLKV VDNIGVIVL DSPGVKVIF VNKILILIL MKMNGKVLE IGSTINIER SDGRVHPAI VSSINYELD IVGVEWFEA GETKGKEID FNCVTRLNP HIAAIKPDV QRFSECSSD VALVEKPKK RQSIAVYNN RVKSSKSRI TQDIVKPKN SLKDKYPQT SRSVIEHIS NSFESNVVK KVGKLELER IERRKKQAE FKAEKVELT KRNADNPHW ELHQMIHDY CKNLNYKPL TINDVIEDR LITVCVRKR PLNKSENAK KEVDVITVP SKNQIIVHE PKTKVDLTK YLENHLFKF DYTFNEHCD NEIVYKYTA QPLIRTVFE GGFATCFAY GQTGSGKTY TMSGDIFRT NAHKGIYAM AATDVFRLV ASPKYRHLN LIVTCSFFE IYSRKVLDL LNRKGDLKI LEDGKQQVQ VVGLTEKVV SSVNEVLEL INHGNMART SGQTSANEN SSRSHAVFQ IYLRPAKKF NSLHGKFSL IDLAGNERG ADTLSSTKT TRMEGGEIN KSLLALKEC IRALGRKGA HLPFRLSKL TQVLRDSFI GANSKTCMI AMVSPGVSS CENTLNTLR YADRVKELG GGELQHLST INEKNNEII SMGLEEEKS FSDNSEEFK IVKEKVVNL HREIISKLQ DATKEAKYL NKCIDNEPE KYCREFEEF VDDTVAFLL NVKNSVIEY KMKLLAEE
- >TC033224 RA
atgtcgaca ctacggttt tttggggcc ttatcgaaa atttcccgt actaacata aagtttaag aacacagca caaatccgc ggcatggcc tcacaggag aggaagcac acgaagctc aaagttgtg gataatatt ggagtcata gtcttagac tcgccagga gtcaaagtg atatttgtc aacaaaatt ttgattttg attttaatg aaaatgaac gggaaagtt ctcgaaatc ggttccaca atcaacatc gagcgaagt gacggtaga gtgcatccg gcaatcgta tccagcatt aactacgag ctggacata gtcggggtt gagtggttt gaggcaggt gaaaccaaa ggcaaggaa atcgacttt aattgcgtc accagacta aaccctcac atcgccgct ataaagcca gacgttcag agattcagt gagtgttcc tctgacgtt gctctagtc gaaaaaccg aaaaaacgc cagtctatc gccgtgtac aacaatcgc gtcaaatcc tccaagtcg agaatcacc caagacatc gttaagccg aaaaattca ctcaaagac aagtacccc caaacctcg cggtccgta atcgagcac atctccaac tcgttcgag agcaatgtg gtcaagaaa gtgggaaaa ctggagctc gaacgcatc gagcgccgc aaaaaacag gcggaattc aaagcggaa aaagtcgag ctgaccaaa cgtaacgcc gataacccc cattgggaa ttgcaccaa atgatccac gattactgt aaaaacctg aactataaa cctttgacg atcaatgat gtaatagag gaccgtctc ataacagtc tgcgtccgc aagcgacct ttgaacaag agcgaaaac gcgaagaaa gaagtcgac gttataaca gtgcccagc aaaaaccaa atcatcgtt cacgaaccc aaaaccaaa gtcgatctc accaaatac ctcgaaaac cacttattc aaattcgat tacacgttc aacgagcat tgcgacaac gaaatcgtc tacaaatac acggcgcaa ccactcatc cggacggtt tttgagggc ggtttcgcc acgtgtttc gcatacgga cagaccgga tccgggaaa acgtacaca atgagcggc gatattttc cgaacgaat gcccacaag ggaatttat gcgatggct gcgaccgac gttttccga cttgtagcg tccccaaaa taccggcat ttaaacttg attgtaacg tgcagtttc ttcgagatt tactcgcgc aaagtgctc gatttgttg aaccgtaaa ggcgattta aaaatcctg gaagacggc aagcagcag gtccaagtt gtgggcctc accgaaaag gttgtgtcc tcagttaat gaagttctg gagttgatt aatcacggg aatatggcc aggacttcg gggcaaacc tcggcgaac gaaaactcc tcgcgttcg catgccgtt ttccagatt tatttgcgt ccggcgaag aaatttaat tcgctgcat ggaaaattc tctttgatt gatttggcg gggaacgag cgcggggcc gacactttg tcgtccact aagacaacg cgaatggaa gggggcgag ataaacaag tcgcttttg gctttgaag gagtgtata agggccctg gggaggaaa ggggctcat ttgccgttt cggttgagt aaattaacg caggttttg cgcgattcg ttcatcggg gccaattcg aagacttgc atgattgcg atggtgtct cctggagtc agttcgtgc gaaaatacg ttgaatacg ttgcggtac gctgaccgg gttaaggag ctagggggc ggcgagttg cagcatttg tcgacgatt aatgagaaa aataacgag attatttcg atggggttg gaggaggag aagagtttc tcggataat tccgaagag tttaaaatt gttaaagaa aaagttgtt aatctacac agagagatt attagtaaa ctgcaagat gcgaccaag gaggccaag tatttgaat aagtgtatt gacaacgaa ccggagaag tactgtcgg gaattcgaa gagtttgtc gacgatact gtggcgttt ttgttgaat gttaaaaat agtgtcatt gaatataaa atgaagctg ttggccgaa gaatga
- >TC033224 RB
atgtcgaca ctacggttt tttggggcc ttatcgaaa atttcccgt actaacata aagtttaag aacacaggg gccttttca gcacaaatc cgcggcatg gcctcacag gagaggaag cacacgaag ctcaaagtt gtggataat attggagtc atagtctta gactcgcca ggagtcaaa gtgatattt gtcaacaaa attttgatt ttgatttta atgaaaatg aacgggaaa gttctcgaa atcggttcc acaatcaac atcgagcga agtgacggt agagtgcat ccggcaatc gtatccagc attaactac gagctggac atagtcggg gttgagtgg tttgaggca ggtgaaacc aaaggcaag gaaatcgac tttaattgc gtcaccaga ctaaaccct cacatcgcc gctataaag ccagacgtt cagagattc agtgagtgt tcctctgac gttgctcta gtcgaaaaa ccgaaaaaa cgccagtct atcgccgtg tacaacaat cgcgtcaaa tcctccaag tcgagaatc acccaagac atcgttaag ccgaaaaat tcactcaaa gacaagtac ccccaaacc tcgcggtcc gtaatcgag cacatctcc aactcgttc gagagcaat gtggtcaag aaagtggga aaactggag ctcgaacgc atcgagcgc cgcaaaaaa caggcggaa ttcaaagcg gaaaaagtc gagctgacc aaacgtaac gccgataac ccccattgg gaattgcac caaatgatc cacgattac tgtaaaaac ctgaactat aaacctttg acgatcaat gatgtaata gaggaccgt ctcataaca gtctgcgtc cgcaagcga cctttgaac aagagcgaa aacgcgaag aaagaagtc gacgttata acagtgccc agcaaaaac caaatcatc gttcacgaa cccaaaacc aaagtcgat ctcaccaaa tacctcgaa aaccactta ttcaaattc gattacacg ttcaacgag cattgcgac aacgaaatc gtctacaaa tacacggcg caaccactc atccggacg gtttttgag ggcggtttc gccacgtgt ttcgcatac ggacagacc ggatccggg aaaacgtac acaatgagc ggcgatatt ttccgaacg aatgcccac aagggaatt tatgcgatg gctgcgacc gacgttttc cgacttgta gcgtcccca aaataccgg catttaaac ttgattgta acgtgcagt ttcttcgag atttactcg cgcaaagtg ctcgatttg ttgaaccgt aaaggcgat ttaaaaatc ctggaagac ggcaagcag caggtccaa gttgtgggc ctcaccgaa aaggttgtg tcctcagtt aatgaagtt ctggagttg attaatcac gggaatatg gccaggact tcggggcaa acctcggcg aacgaaaac tcctcgcgt tcgcatgcc gttttccag atttatttg cgtccggcg aagaaattt aattcgctg catggaaaa ttctctttg attgatttg gcggggaac gagcgcggg gccgacact ttgtcgtcc actaagaca acgcgaatg gaagggggc gagataaac aagtcgctt ttggctttg aaggagtgt ataagggcc ctggggagg aaaggggct catttgccg tttcggttg agtaaatta acgcaggtt ttgcgcgat tcgttcatc ggggccaat tcgaagact tgcatgatt gcgatggtg tctcctgga gtcagttcg tgcgaaaat acgttgaat acgttgcgg tacgctgac cgggttaag gagctaggg ggcggcgag ttgcagcat ttgtcgacg attaatgag aaaaataac gagattatt tcgatgggg ttggaggag gagaagagt ttctcggat aattccgaa gagtttaaa attgttaaa gaaaaagtt gttaatcta cacagagag attattagt aaactgcaa gatgcgacc aaggaggcc aagtatttg aataagtgt attgacaac gaaccggag aagtactgt cgggaattc gaagagttt gtcgacgat actgtggcg tttttgttg aatgttaaa aatagtgtc attgaatat aaaatgaag ctgttggcc gaagaatga
- >TC033224 RA
gtcaatgtc aaacccaca tgatcaaaa gtcatgaat gcataaaat agttatcca actgggcaa agttcatta ctcggctca ttttgaaaa gctggggaa gtgtaggga gtgttggtg tttgtagtt gctcgaaaa ttcaattaa aattggaac catgtcgac actacggtt ttttggggc cttatcgaa aatttcccg tactaacat aaagtttaa gaacacagc acaaatccg cggcatggc ctcacagga gaggaagca cacgaagct caaagttgt ggataatat tggagtcat agtcttaga ctcgccagg agtcaaagt gatatttgt caacaaaat tttgatttt gattttaat gaaaatgaa cgggaaagt tctcgaaat cggttccac aatcaacat cgagcgaag tgacggtag agtgcatcc ggcaatcgt atccagcat taactacga gctggacat agtcggggt tgagtggtt tgaggcagg tgaaaccaa aggcaagga aatcgactt taattgcgt caccagact aaaccctca catcgccgc tataaagcc agacgttca gagattcag tgagtgttc ctctgacgt tgctctagt cgaaaaacc gaaaaaacg ccagtctat cgccgtgta caacaatcg cgtcaaatc ctccaagtc gagaatcac ccaagacat cgttaagcc gaaaaattc actcaaaga caagtaccc ccaaacctc gcggtccgt aatcgagca catctccaa ctcgttcga gagcaatgt ggtcaagaa agtgggaaa actggagct cgaacgcat cgagcgccg caaaaaaca ggcggaatt caaagcgga aaaagtcga gctgaccaa acgtaacgc cgataaccc ccattggga attgcacca aatgatcca cgattactg taaaaacct gaactataa acctttgac gatcaatga tgtaataga ggaccgtct cataacagt ctgcgtccg caagcgacc tttgaacaa gagcgaaaa cgcgaagaa agaagtcga cgttataac agtgcccag caaaaacca aatcatcgt tcacgaacc caaaaccaa agtcgatct caccaaata cctcgaaaa ccacttatt caaattcga ttacacgtt caacgagca ttgcgacaa cgaaatcgt ctacaaata cacggcgca accactcat ccggacggt ttttgaggg cggtttcgc cacgtgttt cgcatacgg acagaccgg atccgggaa aacgtacac aatgagcgg cgatatttt ccgaacgaa tgcccacaa gggaattta tgcgatggc tgcgaccga cgttttccg acttgtagc gtccccaaa ataccggca tttaaactt gattgtaac gtgcagttt cttcgagat ttactcgcg caaagtgct cgatttgtt gaaccgtaa aggcgattt aaaaatcct ggaagacgg caagcagca ggtccaagt tgtgggcct caccgaaaa ggttgtgtc ctcagttaa tgaagttct ggagttgat taatcacgg gaatatggc caggacttc ggggcaaac ctcggcgaa cgaaaactc ctcgcgttc gcatgccgt tttccagat ttatttgcg tccggcgaa gaaatttaa ttcgctgca tggaaaatt ctctttgat tgatttggc ggggaacga gcgcggggc cgacacttt gtcgtccac taagacaac gcgaatgga agggggcga gataaacaa gtcgctttt ggctttgaa ggagtgtat aagggccct ggggaggaa aggggctca tttgccgtt tcggttgag taaattaac gcaggtttt gcgcgattc gttcatcgg ggccaattc gaagacttg catgattgc gatggtgtc tcctggagt cagttcgtg cgaaaatac gttgaatac gttgcggta cgctgaccg ggttaagga gctaggggg cggcgagtt gcagcattt gtcgacgat taatgagaa aaataacga gattatttc gatggggtt ggaggagga gaagagttt ctcggataa ttccgaaga gtttaaaat tgttaaaga aaaagttgt taatctaca cagagagat tattagtaa actgcaaga tgcgaccaa ggaggccaa gtatttgaa taagtgtat tgacaacga accggagaa gtactgtcg ggaattcga agagtttgt cgacgatac tgtggcgtt tttgttgaa tgttaaaaa tagtgtcat tgaatataa aatgaagct gttggccga agaatgatt ttttgcaat aaattgtct ggcaagaa
- >TC033224 RB
gtcaatgtc aaacccaca tgatcaaaa gtcatgaat gcataaaat agttatcca actgggcaa agttcatta ctcggctca ttttgaaaa gctggggaa gtgtaggga gtgttggtg tttgtagtt gctcgaaaa ttcaattaa aattggaac catgtcgac actacggtt ttttggggc cttatcgaa aatttcccg tactaacat aaagtttaa gaacacagg ggccttttc agcacaaat ccgcggcat ggcctcaca ggagaggaa gcacacgaa gctcaaagt tgtggataa tattggagt catagtctt agactcgcc aggagtcaa agtgatatt tgtcaacaa aattttgat tttgatttt aatgaaaat gaacgggaa agttctcga aatcggttc cacaatcaa catcgagcg aagtgacgg tagagtgca tccggcaat cgtatccag cattaacta cgagctgga catagtcgg ggttgagtg gtttgaggc aggtgaaac caaaggcaa ggaaatcga ctttaattg cgtcaccag actaaaccc tcacatcgc cgctataaa gccagacgt tcagagatt cagtgagtg ttcctctga cgttgctct agtcgaaaa accgaaaaa acgccagtc tatcgccgt gtacaacaa tcgcgtcaa atcctccaa gtcgagaat cacccaaga catcgttaa gccgaaaaa ttcactcaa agacaagta cccccaaac ctcgcggtc cgtaatcga gcacatctc caactcgtt cgagagcaa tgtggtcaa gaaagtggg aaaactgga gctcgaacg catcgagcg ccgcaaaaa acaggcgga attcaaagc ggaaaaagt cgagctgac caaacgtaa cgccgataa cccccattg ggaattgca ccaaatgat ccacgatta ctgtaaaaa cctgaacta taaaccttt gacgatcaa tgatgtaat agaggaccg tctcataac agtctgcgt ccgcaagcg acctttgaa caagagcga aaacgcgaa gaaagaagt cgacgttat aacagtgcc cagcaaaaa ccaaatcat cgttcacga acccaaaac caaagtcga tctcaccaa atacctcga aaaccactt attcaaatt cgattacac gttcaacga gcattgcga caacgaaat cgtctacaa atacacggc gcaaccact catccggac ggtttttga gggcggttt cgccacgtg tttcgcata cggacagac cggatccgg gaaaacgta cacaatgag cggcgatat tttccgaac gaatgccca caagggaat ttatgcgat ggctgcgac cgacgtttt ccgacttgt agcgtcccc aaaataccg gcatttaaa cttgattgt aacgtgcag tttcttcga gatttactc gcgcaaagt gctcgattt gttgaaccg taaaggcga tttaaaaat cctggaaga cggcaagca gcaggtcca agttgtggg cctcaccga aaaggttgt gtcctcagt taatgaagt tctggagtt gattaatca cgggaatat ggccaggac ttcggggca aacctcggc gaacgaaaa ctcctcgcg ttcgcatgc cgttttcca gatttattt gcgtccggc gaagaaatt taattcgct gcatggaaa attctcttt gattgattt ggcggggaa cgagcgcgg ggccgacac tttgtcgtc cactaagac aacgcgaat ggaaggggg cgagataaa caagtcgct tttggcttt gaaggagtg tataagggc cctggggag gaaaggggc tcatttgcc gtttcggtt gagtaaatt aacgcaggt tttgcgcga ttcgttcat cggggccaa ttcgaagac ttgcatgat tgcgatggt gtctcctgg agtcagttc gtgcgaaaa tacgttgaa tacgttgcg gtacgctga ccgggttaa ggagctagg gggcggcga gttgcagca tttgtcgac gattaatga gaaaaataa cgagattat ttcgatggg gttggagga ggagaagag tttctcgga taattccga agagtttaa aattgttaa agaaaaagt tgttaatct acacagaga gattattag taaactgca agatgcgac caaggaggc caagtattt gaataagtg tattgacaa cgaaccgga gaagtactg tcgggaatt cgaagagtt tgtcgacga tactgtggc gtttttgtt gaatgttaa aaatagtgt cattgaata taaaatgaa gctgttggc cgaagaatg attttttgc aataaattg tctggcaag aa
