Status
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Gene information
General information
TC033224
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014678,TC014679
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13387223..13389769
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033224
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033224 RA
      MSTLRFFGA LSKISRTNI KFKNTAQIR GMASQERKH 
      TKLKVVDNI GVIVLDSPG VKVIFVNKI LILILMKMN 
      GKVLEIGST INIERSDGR VHPAIVSSI NYELDIVGV 
      EWFEAGETK GKEIDFNCV TRLNPHIAA IKPDVQRFS 
      ECSSDVALV EKPKKRQSI AVYNNRVKS SKSRITQDI 
      VKPKNSLKD KYPQTSRSV IEHISNSFE SNVVKKVGK 
      LELERIERR KKQAEFKAE KVELTKRNA DNPHWELHQ 
      MIHDYCKNL NYKPLTIND VIEDRLITV CVRKRPLNK 
      SENAKKEVD VITVPSKNQ IIVHEPKTK VDLTKYLEN 
      HLFKFDYTF NEHCDNEIV YKYTAQPLI RTVFEGGFA 
      TCFAYGQTG SGKTYTMSG DIFRTNAHK GIYAMAATD 
      VFRLVASPK YRHLNLIVT CSFFEIYSR KVLDLLNRK 
      GDLKILEDG KQQVQVVGL TEKVVSSVN EVLELINHG 
      NMARTSGQT SANENSSRS HAVFQIYLR PAKKFNSLH 
      GKFSLIDLA GNERGADTL SSTKTTRME GGEINKSLL 
      ALKECIRAL GRKGAHLPF RLSKLTQVL RDSFIGANS 
      KTCMIAMVS PGVSSCENT LNTLRYADR VKELGGGEL 
      QHLSTINEK NNEIISMGL EEEKSFSDN SEEFKIVKE 
      KVVNLHREI ISKLQDATK EAKYLNKCI DNEPEKYCR 
      EFEEFVDDT VAFLLNVKN SVIEYKMKL LAEE
    • >TC033224 RB
      MSTLRFFGA LSKISRTNI KFKNTGAFS AQIRGMASQ 
      ERKHTKLKV VDNIGVIVL DSPGVKVIF VNKILILIL 
      MKMNGKVLE IGSTINIER SDGRVHPAI VSSINYELD 
      IVGVEWFEA GETKGKEID FNCVTRLNP HIAAIKPDV 
      QRFSECSSD VALVEKPKK RQSIAVYNN RVKSSKSRI 
      TQDIVKPKN SLKDKYPQT SRSVIEHIS NSFESNVVK 
      KVGKLELER IERRKKQAE FKAEKVELT KRNADNPHW 
      ELHQMIHDY CKNLNYKPL TINDVIEDR LITVCVRKR 
      PLNKSENAK KEVDVITVP SKNQIIVHE PKTKVDLTK 
      YLENHLFKF DYTFNEHCD NEIVYKYTA QPLIRTVFE 
      GGFATCFAY GQTGSGKTY TMSGDIFRT NAHKGIYAM 
      AATDVFRLV ASPKYRHLN LIVTCSFFE IYSRKVLDL 
      LNRKGDLKI LEDGKQQVQ VVGLTEKVV SSVNEVLEL 
      INHGNMART SGQTSANEN SSRSHAVFQ IYLRPAKKF 
      NSLHGKFSL IDLAGNERG ADTLSSTKT TRMEGGEIN 
      KSLLALKEC IRALGRKGA HLPFRLSKL TQVLRDSFI 
      GANSKTCMI AMVSPGVSS CENTLNTLR YADRVKELG 
      GGELQHLST INEKNNEII SMGLEEEKS FSDNSEEFK 
      IVKEKVVNL HREIISKLQ DATKEAKYL NKCIDNEPE 
      KYCREFEEF VDDTVAFLL NVKNSVIEY KMKLLAEE
    CDS sequence
    • >TC033224 RA
      atgtcgaca ctacggttt tttggggcc ttatcgaaa 
      atttcccgt actaacata aagtttaag aacacagca 
      caaatccgc ggcatggcc tcacaggag aggaagcac 
      acgaagctc aaagttgtg gataatatt ggagtcata 
      gtcttagac tcgccagga gtcaaagtg atatttgtc 
      aacaaaatt ttgattttg attttaatg aaaatgaac 
      gggaaagtt ctcgaaatc ggttccaca atcaacatc 
      gagcgaagt gacggtaga gtgcatccg gcaatcgta 
      tccagcatt aactacgag ctggacata gtcggggtt 
      gagtggttt gaggcaggt gaaaccaaa ggcaaggaa 
      atcgacttt aattgcgtc accagacta aaccctcac 
      atcgccgct ataaagcca gacgttcag agattcagt 
      gagtgttcc tctgacgtt gctctagtc gaaaaaccg 
      aaaaaacgc cagtctatc gccgtgtac aacaatcgc 
      gtcaaatcc tccaagtcg agaatcacc caagacatc 
      gttaagccg aaaaattca ctcaaagac aagtacccc 
      caaacctcg cggtccgta atcgagcac atctccaac 
      tcgttcgag agcaatgtg gtcaagaaa gtgggaaaa 
      ctggagctc gaacgcatc gagcgccgc aaaaaacag 
      gcggaattc aaagcggaa aaagtcgag ctgaccaaa 
      cgtaacgcc gataacccc cattgggaa ttgcaccaa 
      atgatccac gattactgt aaaaacctg aactataaa 
      cctttgacg atcaatgat gtaatagag gaccgtctc 
      ataacagtc tgcgtccgc aagcgacct ttgaacaag 
      agcgaaaac gcgaagaaa gaagtcgac gttataaca 
      gtgcccagc aaaaaccaa atcatcgtt cacgaaccc 
      aaaaccaaa gtcgatctc accaaatac ctcgaaaac 
      cacttattc aaattcgat tacacgttc aacgagcat 
      tgcgacaac gaaatcgtc tacaaatac acggcgcaa 
      ccactcatc cggacggtt tttgagggc ggtttcgcc 
      acgtgtttc gcatacgga cagaccgga tccgggaaa 
      acgtacaca atgagcggc gatattttc cgaacgaat 
      gcccacaag ggaatttat gcgatggct gcgaccgac 
      gttttccga cttgtagcg tccccaaaa taccggcat 
      ttaaacttg attgtaacg tgcagtttc ttcgagatt 
      tactcgcgc aaagtgctc gatttgttg aaccgtaaa 
      ggcgattta aaaatcctg gaagacggc aagcagcag 
      gtccaagtt gtgggcctc accgaaaag gttgtgtcc 
      tcagttaat gaagttctg gagttgatt aatcacggg 
      aatatggcc aggacttcg gggcaaacc tcggcgaac 
      gaaaactcc tcgcgttcg catgccgtt ttccagatt 
      tatttgcgt ccggcgaag aaatttaat tcgctgcat 
      ggaaaattc tctttgatt gatttggcg gggaacgag 
      cgcggggcc gacactttg tcgtccact aagacaacg 
      cgaatggaa gggggcgag ataaacaag tcgcttttg 
      gctttgaag gagtgtata agggccctg gggaggaaa 
      ggggctcat ttgccgttt cggttgagt aaattaacg 
      caggttttg cgcgattcg ttcatcggg gccaattcg 
      aagacttgc atgattgcg atggtgtct cctggagtc 
      agttcgtgc gaaaatacg ttgaatacg ttgcggtac 
      gctgaccgg gttaaggag ctagggggc ggcgagttg 
      cagcatttg tcgacgatt aatgagaaa aataacgag 
      attatttcg atggggttg gaggaggag aagagtttc 
      tcggataat tccgaagag tttaaaatt gttaaagaa 
      aaagttgtt aatctacac agagagatt attagtaaa 
      ctgcaagat gcgaccaag gaggccaag tatttgaat 
      aagtgtatt gacaacgaa ccggagaag tactgtcgg 
      gaattcgaa gagtttgtc gacgatact gtggcgttt 
      ttgttgaat gttaaaaat agtgtcatt gaatataaa 
      atgaagctg ttggccgaa gaatga
    • >TC033224 RB
      atgtcgaca ctacggttt tttggggcc ttatcgaaa 
      atttcccgt actaacata aagtttaag aacacaggg 
      gccttttca gcacaaatc cgcggcatg gcctcacag 
      gagaggaag cacacgaag ctcaaagtt gtggataat 
      attggagtc atagtctta gactcgcca ggagtcaaa 
      gtgatattt gtcaacaaa attttgatt ttgatttta 
      atgaaaatg aacgggaaa gttctcgaa atcggttcc 
      acaatcaac atcgagcga agtgacggt agagtgcat 
      ccggcaatc gtatccagc attaactac gagctggac 
      atagtcggg gttgagtgg tttgaggca ggtgaaacc 
      aaaggcaag gaaatcgac tttaattgc gtcaccaga 
      ctaaaccct cacatcgcc gctataaag ccagacgtt 
      cagagattc agtgagtgt tcctctgac gttgctcta 
      gtcgaaaaa ccgaaaaaa cgccagtct atcgccgtg 
      tacaacaat cgcgtcaaa tcctccaag tcgagaatc 
      acccaagac atcgttaag ccgaaaaat tcactcaaa 
      gacaagtac ccccaaacc tcgcggtcc gtaatcgag 
      cacatctcc aactcgttc gagagcaat gtggtcaag 
      aaagtggga aaactggag ctcgaacgc atcgagcgc 
      cgcaaaaaa caggcggaa ttcaaagcg gaaaaagtc 
      gagctgacc aaacgtaac gccgataac ccccattgg 
      gaattgcac caaatgatc cacgattac tgtaaaaac 
      ctgaactat aaacctttg acgatcaat gatgtaata 
      gaggaccgt ctcataaca gtctgcgtc cgcaagcga 
      cctttgaac aagagcgaa aacgcgaag aaagaagtc 
      gacgttata acagtgccc agcaaaaac caaatcatc 
      gttcacgaa cccaaaacc aaagtcgat ctcaccaaa 
      tacctcgaa aaccactta ttcaaattc gattacacg 
      ttcaacgag cattgcgac aacgaaatc gtctacaaa 
      tacacggcg caaccactc atccggacg gtttttgag 
      ggcggtttc gccacgtgt ttcgcatac ggacagacc 
      ggatccggg aaaacgtac acaatgagc ggcgatatt 
      ttccgaacg aatgcccac aagggaatt tatgcgatg 
      gctgcgacc gacgttttc cgacttgta gcgtcccca 
      aaataccgg catttaaac ttgattgta acgtgcagt 
      ttcttcgag atttactcg cgcaaagtg ctcgatttg 
      ttgaaccgt aaaggcgat ttaaaaatc ctggaagac 
      ggcaagcag caggtccaa gttgtgggc ctcaccgaa 
      aaggttgtg tcctcagtt aatgaagtt ctggagttg 
      attaatcac gggaatatg gccaggact tcggggcaa 
      acctcggcg aacgaaaac tcctcgcgt tcgcatgcc 
      gttttccag atttatttg cgtccggcg aagaaattt 
      aattcgctg catggaaaa ttctctttg attgatttg 
      gcggggaac gagcgcggg gccgacact ttgtcgtcc 
      actaagaca acgcgaatg gaagggggc gagataaac 
      aagtcgctt ttggctttg aaggagtgt ataagggcc 
      ctggggagg aaaggggct catttgccg tttcggttg 
      agtaaatta acgcaggtt ttgcgcgat tcgttcatc 
      ggggccaat tcgaagact tgcatgatt gcgatggtg 
      tctcctgga gtcagttcg tgcgaaaat acgttgaat 
      acgttgcgg tacgctgac cgggttaag gagctaggg 
      ggcggcgag ttgcagcat ttgtcgacg attaatgag 
      aaaaataac gagattatt tcgatgggg ttggaggag 
      gagaagagt ttctcggat aattccgaa gagtttaaa 
      attgttaaa gaaaaagtt gttaatcta cacagagag 
      attattagt aaactgcaa gatgcgacc aaggaggcc 
      aagtatttg aataagtgt attgacaac gaaccggag 
      aagtactgt cgggaattc gaagagttt gtcgacgat 
      actgtggcg tttttgttg aatgttaaa aatagtgtc 
      attgaatat aaaatgaag ctgttggcc gaagaatga
    mRNA sequence
    • >TC033224 RA
      gtcaatgtc aaacccaca tgatcaaaa gtcatgaat 
      gcataaaat agttatcca actgggcaa agttcatta 
      ctcggctca ttttgaaaa gctggggaa gtgtaggga 
      gtgttggtg tttgtagtt gctcgaaaa ttcaattaa 
      aattggaac catgtcgac actacggtt ttttggggc 
      cttatcgaa aatttcccg tactaacat aaagtttaa 
      gaacacagc acaaatccg cggcatggc ctcacagga 
      gaggaagca cacgaagct caaagttgt ggataatat 
      tggagtcat agtcttaga ctcgccagg agtcaaagt 
      gatatttgt caacaaaat tttgatttt gattttaat 
      gaaaatgaa cgggaaagt tctcgaaat cggttccac 
      aatcaacat cgagcgaag tgacggtag agtgcatcc 
      ggcaatcgt atccagcat taactacga gctggacat 
      agtcggggt tgagtggtt tgaggcagg tgaaaccaa 
      aggcaagga aatcgactt taattgcgt caccagact 
      aaaccctca catcgccgc tataaagcc agacgttca 
      gagattcag tgagtgttc ctctgacgt tgctctagt 
      cgaaaaacc gaaaaaacg ccagtctat cgccgtgta 
      caacaatcg cgtcaaatc ctccaagtc gagaatcac 
      ccaagacat cgttaagcc gaaaaattc actcaaaga 
      caagtaccc ccaaacctc gcggtccgt aatcgagca 
      catctccaa ctcgttcga gagcaatgt ggtcaagaa 
      agtgggaaa actggagct cgaacgcat cgagcgccg 
      caaaaaaca ggcggaatt caaagcgga aaaagtcga 
      gctgaccaa acgtaacgc cgataaccc ccattggga 
      attgcacca aatgatcca cgattactg taaaaacct 
      gaactataa acctttgac gatcaatga tgtaataga 
      ggaccgtct cataacagt ctgcgtccg caagcgacc 
      tttgaacaa gagcgaaaa cgcgaagaa agaagtcga 
      cgttataac agtgcccag caaaaacca aatcatcgt 
      tcacgaacc caaaaccaa agtcgatct caccaaata 
      cctcgaaaa ccacttatt caaattcga ttacacgtt 
      caacgagca ttgcgacaa cgaaatcgt ctacaaata 
      cacggcgca accactcat ccggacggt ttttgaggg 
      cggtttcgc cacgtgttt cgcatacgg acagaccgg 
      atccgggaa aacgtacac aatgagcgg cgatatttt 
      ccgaacgaa tgcccacaa gggaattta tgcgatggc 
      tgcgaccga cgttttccg acttgtagc gtccccaaa 
      ataccggca tttaaactt gattgtaac gtgcagttt 
      cttcgagat ttactcgcg caaagtgct cgatttgtt 
      gaaccgtaa aggcgattt aaaaatcct ggaagacgg 
      caagcagca ggtccaagt tgtgggcct caccgaaaa 
      ggttgtgtc ctcagttaa tgaagttct ggagttgat 
      taatcacgg gaatatggc caggacttc ggggcaaac 
      ctcggcgaa cgaaaactc ctcgcgttc gcatgccgt 
      tttccagat ttatttgcg tccggcgaa gaaatttaa 
      ttcgctgca tggaaaatt ctctttgat tgatttggc 
      ggggaacga gcgcggggc cgacacttt gtcgtccac 
      taagacaac gcgaatgga agggggcga gataaacaa 
      gtcgctttt ggctttgaa ggagtgtat aagggccct 
      ggggaggaa aggggctca tttgccgtt tcggttgag 
      taaattaac gcaggtttt gcgcgattc gttcatcgg 
      ggccaattc gaagacttg catgattgc gatggtgtc 
      tcctggagt cagttcgtg cgaaaatac gttgaatac 
      gttgcggta cgctgaccg ggttaagga gctaggggg 
      cggcgagtt gcagcattt gtcgacgat taatgagaa 
      aaataacga gattatttc gatggggtt ggaggagga 
      gaagagttt ctcggataa ttccgaaga gtttaaaat 
      tgttaaaga aaaagttgt taatctaca cagagagat 
      tattagtaa actgcaaga tgcgaccaa ggaggccaa 
      gtatttgaa taagtgtat tgacaacga accggagaa 
      gtactgtcg ggaattcga agagtttgt cgacgatac 
      tgtggcgtt tttgttgaa tgttaaaaa tagtgtcat 
      tgaatataa aatgaagct gttggccga agaatgatt 
      ttttgcaat aaattgtct ggcaagaa
    • >TC033224 RB
      gtcaatgtc aaacccaca tgatcaaaa gtcatgaat 
      gcataaaat agttatcca actgggcaa agttcatta 
      ctcggctca ttttgaaaa gctggggaa gtgtaggga 
      gtgttggtg tttgtagtt gctcgaaaa ttcaattaa 
      aattggaac catgtcgac actacggtt ttttggggc 
      cttatcgaa aatttcccg tactaacat aaagtttaa 
      gaacacagg ggccttttc agcacaaat ccgcggcat 
      ggcctcaca ggagaggaa gcacacgaa gctcaaagt 
      tgtggataa tattggagt catagtctt agactcgcc 
      aggagtcaa agtgatatt tgtcaacaa aattttgat 
      tttgatttt aatgaaaat gaacgggaa agttctcga 
      aatcggttc cacaatcaa catcgagcg aagtgacgg 
      tagagtgca tccggcaat cgtatccag cattaacta 
      cgagctgga catagtcgg ggttgagtg gtttgaggc 
      aggtgaaac caaaggcaa ggaaatcga ctttaattg 
      cgtcaccag actaaaccc tcacatcgc cgctataaa 
      gccagacgt tcagagatt cagtgagtg ttcctctga 
      cgttgctct agtcgaaaa accgaaaaa acgccagtc 
      tatcgccgt gtacaacaa tcgcgtcaa atcctccaa 
      gtcgagaat cacccaaga catcgttaa gccgaaaaa 
      ttcactcaa agacaagta cccccaaac ctcgcggtc 
      cgtaatcga gcacatctc caactcgtt cgagagcaa 
      tgtggtcaa gaaagtggg aaaactgga gctcgaacg 
      catcgagcg ccgcaaaaa acaggcgga attcaaagc 
      ggaaaaagt cgagctgac caaacgtaa cgccgataa 
      cccccattg ggaattgca ccaaatgat ccacgatta 
      ctgtaaaaa cctgaacta taaaccttt gacgatcaa 
      tgatgtaat agaggaccg tctcataac agtctgcgt 
      ccgcaagcg acctttgaa caagagcga aaacgcgaa 
      gaaagaagt cgacgttat aacagtgcc cagcaaaaa 
      ccaaatcat cgttcacga acccaaaac caaagtcga 
      tctcaccaa atacctcga aaaccactt attcaaatt 
      cgattacac gttcaacga gcattgcga caacgaaat 
      cgtctacaa atacacggc gcaaccact catccggac 
      ggtttttga gggcggttt cgccacgtg tttcgcata 
      cggacagac cggatccgg gaaaacgta cacaatgag 
      cggcgatat tttccgaac gaatgccca caagggaat 
      ttatgcgat ggctgcgac cgacgtttt ccgacttgt 
      agcgtcccc aaaataccg gcatttaaa cttgattgt 
      aacgtgcag tttcttcga gatttactc gcgcaaagt 
      gctcgattt gttgaaccg taaaggcga tttaaaaat 
      cctggaaga cggcaagca gcaggtcca agttgtggg 
      cctcaccga aaaggttgt gtcctcagt taatgaagt 
      tctggagtt gattaatca cgggaatat ggccaggac 
      ttcggggca aacctcggc gaacgaaaa ctcctcgcg 
      ttcgcatgc cgttttcca gatttattt gcgtccggc 
      gaagaaatt taattcgct gcatggaaa attctcttt 
      gattgattt ggcggggaa cgagcgcgg ggccgacac 
      tttgtcgtc cactaagac aacgcgaat ggaaggggg 
      cgagataaa caagtcgct tttggcttt gaaggagtg 
      tataagggc cctggggag gaaaggggc tcatttgcc 
      gtttcggtt gagtaaatt aacgcaggt tttgcgcga 
      ttcgttcat cggggccaa ttcgaagac ttgcatgat 
      tgcgatggt gtctcctgg agtcagttc gtgcgaaaa 
      tacgttgaa tacgttgcg gtacgctga ccgggttaa 
      ggagctagg gggcggcga gttgcagca tttgtcgac 
      gattaatga gaaaaataa cgagattat ttcgatggg 
      gttggagga ggagaagag tttctcgga taattccga 
      agagtttaa aattgttaa agaaaaagt tgttaatct 
      acacagaga gattattag taaactgca agatgcgac 
      caaggaggc caagtattt gaataagtg tattgacaa 
      cgaaccgga gaagtactg tcgggaatt cgaagagtt 
      tgtcgacga tactgtggc gtttttgtt gaatgttaa 
      aaatagtgt cattgaata taaaatgaa gctgttggc 
      cgaagaatg attttttgc aataaattg tctggcaag 
      aa
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