Status
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Gene information
General information
TC033217
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014668
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13217196..13228840
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033217
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05671

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    Pupal morphology
    head irregular - Number of animals showing the phenotype: 8

    Comment: the head is translucent in transmitted light, although the ds-red expression in the cns is comparable to WT
    legs slightly orientation irregular - Number of animals showing the phenotype: 7

    wings slightly size decreased - Number of animals showing the phenotype: 7
    wings slightly rounded - Number of animals showing the phenotype: 7
    wings ventral not closed - Number of animals showing the phenotype: 7
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033217 RA
      MYYSSLGYI SNATVLFLG AHLRQRSLD TERRRQSLG 
      TIIPHRASD AFLDPYHAA ILFRDARGL PVVDPFLEK 
      VNISDLEED ESQIFVKFF KFHKCYDLI PTSAKLVVF 
      DTQLLVKKA FFALVYNGV RAAPLWDSS QQEFVGMLT 
      ITDFIKILR MYYKSPTVA MDELEEHKL DTWRHVLKD 
      QRPLIYISP DASLYDAIR TLIHNRIHR LPVIDPETG 
      NVLYILTHK RILRFLFLY INELPKPSY MNKTLRDVR 
      IGSYENIET ATEDTSIIL ALKKFVERR VSALPLVDN 
      EGRLVDIYA KFDVINLAA EKTYNDLDV SLKKANEHR 
      NEWFEGVHK CKLDETLFT IMDKIVKAE VHRLVVVDD 
      DDKVIGIIS LSDLFLYLV LRPCGEDGS PDGVASVRA 
      QDIKLQETI SQSERTSST EEVSQTIPE EEEDPTVEK 
      NTQEEKSEN HKDDSDTSL PDSPVIEST TSLPSENSM 
      FREVTVTGG GE
    • >TC033217 RB
      MLRLHIFSN HVRLFLPTI EEEEEFYTI DQIKEFEYV 
      SPEYKEWLA DVLEQEEDE SQIFVKFFK FHKCYDLIP 
      TSAKLVVFD TQLLVKKAF FALVYNGVR AAPLWDSSQ 
      QEFVGMLTI TDFIKILRM YYKSPTVAM DELEEHKLD 
      TWRHVLKDQ RPLIYISPD ASLYDAIRT LIHNRIHRL 
      PVIDPETGN VLYILTHKR ILRFLFLYI NELPKPSYM 
      NKTLRDVRI GSYENIETA TEDTSIILA LKKFVERRV 
      SALPLVDNE GRLVDIYAK FDVINLAAE KTYNDLDVS 
      LKKANEHRN EWFEGVHKC KLDETLFTI MDKIVKAEV 
      HRLVVVDDD DKVIGIISL SDLFLYLVL RPCGEDGSP 
      DGVASVRAQ DIKLQETIS QSERTSSTE EVSQTIPEE 
      EEDPTVEKN TQEEKSENH KDDSDTSLP DSPVIESTT 
      SLPSENSMF REVTVTGGG E
    • >TC033217 RC
      MYYSSLGYI SNATVLFLG AHLRQRSLD TERRRQSLG 
      TIIPHRASD AFLDPYHAA ILFRDARGL PVVDPFLEK 
      VNISDLGSR NALKTRV
    CDS sequence
    • >TC033217 RA
      atgtattat tcatcattg ggttacatt tcaaacgca 
      actgttttg tttttaggg gcgcatttg aggcagaga 
      tctttagat actgaaaga cggcggcaa tctctcgga 
      actatcatt cctcacagg gcttcagac gctttcctc 
      gacccttat catgctgcg attcttttc agggatgca 
      agagggtta ccagtcgtc gatccgttc ctagaaaaa 
      gtcaatata tcggattta gaagaggat gaatctcaa 
      atattcgtc aaattcttc aagttccac aagtgctat 
      gatctcatt cccacctcc gccaagttg gtcgtcttc 
      gacacgcag ctgctggtc aagaaggcc ttcttcgct 
      ctggtctac aacggtgtc cgagcggcg ccgctgtgg 
      gacagcagc cagcaagag ttcgtcggc atgctgacc 
      atcaccgac tttataaaa atcctgcga atgtactac 
      aagtcgcca acggtggcg atggacgag cttgaggag 
      cacaaactg gacacttgg cggcacgtg ttgaaggat 
      cagcgtcct cttatctac atcagtcct gacgcttcg 
      ctctacgat gcaatccgc actcttatc cacaacaga 
      atccacaga ttaccggtg atcgatccg gagacgggg 
      aacgtcctc tacatccta acccacaag aggatactc 
      cgatttttg ttcttatac attaacgaa cttccgaaa 
      cctagttat atgaacaaa acgctgcga gatgtgagg 
      atcggtagt tacgagaat atcgaaaca gcaacggag 
      gacacgtcg attatttta gctttgaag aagttcgtc 
      gagaggcga gtctcggca ctgcctctt gtagataac 
      gaaggtcgg cttgttgat atttacgct aaatttgac 
      gttattaat ttagccgct gaaaagact tataatgat 
      ctagacgtt tcgttaaag aaagctaat gagcaccgg 
      aacgaatgg tttgaaggt gtgcataaa tgtaagctc 
      gacgagacg ttgtttacg attatggac aagattgtc 
      aaagcagag gtgcatagg ctcgtggtt gttgacgac 
      gacgacaaa gttatagga ataatatcc ctgtctgat 
      ctattcctt tacctggtg ttgcgacct tgcggcgaa 
      gacggttct cccgacggt gtggcttcg gtgagggcg 
      caagacata aaactgcag gagacgatt tcgcagtcg 
      gagaggacg tcctccact gaagaagtg tcgcaaaca 
      atccccgaa gaggaggaa gacccgacc gtagagaaa 
      aatactcaa gaagaaaaa agcgaaaac cataaagat 
      gattccgac acgagtctg cccgattct cccgtcatc 
      gaaagtacg acgtcgtta ccgagcgag aacagcatg 
      ttcagagag gtgacggtg acgggaggc ggtgaataa
    • >TC033217 RB
      atgttacgt ttgcacatc ttctcgaat catgtgcgt 
      ctgtttttg cccacgatc gaagaggag gaagagttt 
      tacactatc gatcaaatc aaagagttc gagtacgtc 
      tcgcccgag tacaaagag tggcttgcg gacgtcctc 
      gagcaagaa gaggatgaa tctcaaata ttcgtcaaa 
      ttcttcaag ttccacaag tgctatgat ctcattccc 
      acctccgcc aagttggtc gtcttcgac acgcagctg 
      ctggtcaag aaggccttc ttcgctctg gtctacaac 
      ggtgtccga gcggcgccg ctgtgggac agcagccag 
      caagagttc gtcggcatg ctgaccatc accgacttt 
      ataaaaatc ctgcgaatg tactacaag tcgccaacg 
      gtggcgatg gacgagctt gaggagcac aaactggac 
      acttggcgg cacgtgttg aaggatcag cgtcctctt 
      atctacatc agtcctgac gcttcgctc tacgatgca 
      atccgcact cttatccac aacagaatc cacagatta 
      ccggtgatc gatccggag acggggaac gtcctctac 
      atcctaacc cacaagagg atactccga tttttgttc 
      ttatacatt aacgaactt ccgaaacct agttatatg 
      aacaaaacg ctgcgagat gtgaggatc ggtagttac 
      gagaatatc gaaacagca acggaggac acgtcgatt 
      attttagct ttgaagaag ttcgtcgag aggcgagtc 
      tcggcactg cctcttgta gataacgaa ggtcggctt 
      gttgatatt tacgctaaa tttgacgtt attaattta 
      gccgctgaa aagacttat aatgatcta gacgtttcg 
      ttaaagaaa gctaatgag caccggaac gaatggttt 
      gaaggtgtg cataaatgt aagctcgac gagacgttg 
      tttacgatt atggacaag attgtcaaa gcagaggtg 
      cataggctc gtggttgtt gacgacgac gacaaagtt 
      ataggaata atatccctg tctgatcta ttcctttac 
      ctggtgttg cgaccttgc ggcgaagac ggttctccc 
      gacggtgtg gcttcggtg agggcgcaa gacataaaa 
      ctgcaggag acgatttcg cagtcggag aggacgtcc 
      tccactgaa gaagtgtcg caaacaatc cccgaagag 
      gaggaagac ccgaccgta gagaaaaat actcaagaa 
      gaaaaaagc gaaaaccat aaagatgat tccgacacg 
      agtctgccc gattctccc gtcatcgaa agtacgacg 
      tcgttaccg agcgagaac agcatgttc agagaggtg 
      acggtgacg ggaggcggt gaataa
    • >TC033217 RC
      atgtattat tcatcattg ggttacatt tcaaacgca 
      actgttttg tttttaggg gcgcatttg aggcagaga 
      tctttagat actgaaaga cggcggcaa tctctcgga 
      actatcatt cctcacagg gcttcagac gctttcctc 
      gacccttat catgctgcg attcttttc agggatgca 
      agagggtta ccagtcgtc gatccgttc ctagaaaaa 
      gtcaatata tcggattta ggttcgcgg aacgcacta 
      aaaacgaga gtctaa
    mRNA sequence
    • >TC033217 RA
      tctttgata acaagctta acatgtatt attcatcat 
      tgggttaca tttcaaacg caactgttt tgtttttag 
      gggcgcatt tgaggcaga gatctttag atactgaaa 
      gacggcggc aatctctcg gaactatca ttcctcaca 
      gggcttcag acgctttcc tcgaccctt atcatgctg 
      cgattcttt tcagggatg caagagggt taccagtcg 
      tcgatccgt tcctagaaa aagtcaata tatcggatt 
      tagaagagg atgaatctc aaatattcg tcaaattct 
      tcaagttcc acaagtgct atgatctca ttcccacct 
      ccgccaagt tggtcgtct tcgacacgc agctgctgg 
      tcaagaagg ccttcttcg ctctggtct acaacggtg 
      tccgagcgg cgccgctgt gggacagca gccagcaag 
      agttcgtcg gcatgctga ccatcaccg actttataa 
      aaatcctgc gaatgtact acaagtcgc caacggtgg 
      cgatggacg agcttgagg agcacaaac tggacactt 
      ggcggcacg tgttgaagg atcagcgtc ctcttatct 
      acatcagtc ctgacgctt cgctctacg atgcaatcc 
      gcactctta tccacaaca gaatccaca gattaccgg 
      tgatcgatc cggagacgg ggaacgtcc tctacatcc 
      taacccaca agaggatac tccgatttt tgttcttat 
      acattaacg aacttccga aacctagtt atatgaaca 
      aaacgctgc gagatgtga ggatcggta gttacgaga 
      atatcgaaa cagcaacgg aggacacgt cgattattt 
      tagctttga agaagttcg tcgagaggc gagtctcgg 
      cactgcctc ttgtagata acgaaggtc ggcttgttg 
      atatttacg ctaaatttg acgttatta atttagccg 
      ctgaaaaga cttataatg atctagacg tttcgttaa 
      agaaagcta atgagcacc ggaacgaat ggtttgaag 
      gtgtgcata aatgtaagc tcgacgaga cgttgttta 
      cgattatgg acaagattg tcaaagcag aggtgcata 
      ggctcgtgg ttgttgacg acgacgaca aagttatag 
      gaataatat ccctgtctg atctattcc tttacctgg 
      tgttgcgac cttgcggcg aagacggtt ctcccgacg 
      gtgtggctt cggtgaggg cgcaagaca taaaactgc 
      aggagacga tttcgcagt cggagagga cgtcctcca 
      ctgaagaag tgtcgcaaa caatccccg aagaggagg 
      aagacccga ccgtagaga aaaatactc aagaagaaa 
      aaagcgaaa accataaag atgattccg acacgagtc 
      tgcccgatt ctcccgtca tcgaaagta cgacgtcgt 
      taccgagcg agaacagca tgttcagag aggtgacgg 
      tgacgggag gcggtgaat aattttgtt tttattttc 
      tgggtgatt agtatttaa gatgtgacg gttcatgtt 
      agttttgag ccggtcctg gattgtttc tttgttacg 
      ggacgttgg tcgtttgtt agtcgacaa tgtctgtta 
      ctttgtgat cgcttgtcc taaatttac aagggacta 
      taatatttt taagaaatt tgtacttaa taagttaca 
      taccaaaga ctgctttac gtcatgccg agtgacggg 
      tggaagaaa caattcaga tatcaacta actcatggt 
      gtgctttat ttatttgta taatacact acacttatt 
      ttttgtcag atagtgctg taaaaagag ataaaatgg 
      ttaacatgt tattgttac aactatatc ctataatgt 
      aaaattact caatttagc ttcacggaa cagtgcttt 
      gatgacatt gaagatgaa agtcgggag gaatataaa 
      ttgtttaaa catagtaac aaagaagcc ttatatttc 
      gtacaatag aggatttgt gcttaatgt tatcaaagc 
      agaaaaacc tcaaattaa cactttttt gtaaatact 
      gaagaatgg tggattata tttttaata ttgtgtgga 
      ttttttaat ttcagagct gaaggaaaa gtatcttga 
      tgagatcta caattttta aattttata tataaaaat 
      taagataat gtacagttc gaaataaat gatttaaag 
      tgaggttca ctcaaagtt ggtatttgc taatttatt 
      gttaaggta gtttaagat atacaacga tgtagttac 
      ataatattt agttagcta ggctcatgg cctatacga 
      tgcttagta aa
    • >TC033217 RB
      gtgcggaaa ttttgctcc acttctggc agctgcatt 
      ctgttgatg caatccatc agataatgc agacaggtt 
      tttgcattt ttattttca ttccgacaa gcgttgtgc 
      ttggcactg tgctatcaa tttatggga aatgataca 
      taaaacgag cgcaggaat ttttatttt catctctga 
      cacgacaat taataaagc ttaccggtt gtcgctttg 
      tgcaccgta aatatagaa atgaggttt cacattttt 
      tcagctcat attaaaatt ggtggattc gcaaatgtg 
      ccgttttac tgtgaatta attaaagca cccaaacgg 
      gcaagaaat aaaaaattc tgtgggaaa ggaaatatt 
      tttccgagt ttaagagct ctaatggtt ttaatgctt 
      gttaccgga aaggtgagc tgccttgaa ttcatacat 
      accaatgag ctgtttaat tagtttcca tcgtttaaa 
      gcttgtgtt aaaattggg cgattgtaa gtgaattca 
      gaaatagac tattttggt gaagataag ttatcttac 
      ctcacacgt cattcagtt tcgtttgac cctcttcaa 
      ccttcgtgt aattcaatg tccgtttcg ataatggcc 
      aaaacaaca gaaactgga aaaaggatt attagagca 
      atcgcgatg tttaattgt gctagagcc acctttaac 
      atcacggtc acaaagatc aagtattat gaccctgtg 
      gaagctgta atccaatta caaactaga gtgaatttt 
      tcgcccagt taaatgtca aaagacgac ttgcaaata 
      gcaaataac atcattttt cgttaatga tatcgacga 
      ctggcgacg ctcgttcag tattttggc agctggtcc 
      attcgacgc caataatag cttgacgtt tatccagtc 
      agtgtgaga cggggctcc tggcatggt gagagttaa 
      ttcggattt ggtgaagaa acaaaccgc aaaaagtct 
      tgttcgtac tagtcgcta tctaatgtt gttattgat 
      atttggttc cttgtggcg cgattgaat ttgcgaagg 
      gggagatct tgttatgtc gaaacattt ttaatcagc 
      tgacctccg ataactcgc tcctgtttt gcattaaat 
      tggcgatgt tgggattta tttgttgtc atatgtttg 
      cggaaattt ggcagtagt gtccgtttc ggtggatta 
      atgaaagtg gctgccatg cggtgagct ttgttgaca 
      tttcgcggc gcgtttttg ggggtgttt tgtgcgatg 
      tgggacggg catgttacg tttgcacat cttctcgaa 
      tcatgtgcg tctgttttt gcccacgat cgaagagga 
      ggaagagtt ttacactat cgatcaaat caaagagtt 
      cgagtacgt ctcgcccga gtacaaaga gtggcttgc 
      ggacgtcct cgagcaaga agaggatga atctcaaat 
      attcgtcaa attcttcaa gttccacaa gtgctatga 
      tctcattcc cacctccgc caagttggt cgtcttcga 
      cacgcagct gctggtcaa gaaggcctt cttcgctct 
      ggtctacaa cggtgtccg agcggcgcc gctgtggga 
      cagcagcca gcaagagtt cgtcggcat gctgaccat 
      caccgactt tataaaaat cctgcgaat gtactacaa 
      gtcgccaac ggtggcgat ggacgagct tgaggagca 
      caaactgga cacttggcg gcacgtgtt gaaggatca 
      gcgtcctct tatctacat cagtcctga cgcttcgct 
      ctacgatgc aatccgcac tcttatcca caacagaat 
      ccacagatt accggtgat cgatccgga gacggggaa 
      cgtcctcta catcctaac ccacaagag gatactccg 
      atttttgtt cttatacat taacgaact tccgaaacc 
      tagttatat gaacaaaac gctgcgaga tgtgaggat 
      cggtagtta cgagaatat cgaaacagc aacggagga 
      cacgtcgat tattttagc tttgaagaa gttcgtcga 
      gaggcgagt ctcggcact gcctcttgt agataacga 
      aggtcggct tgttgatat ttacgctaa atttgacgt 
      tattaattt agccgctga aaagactta taatgatct 
      agacgtttc gttaaagaa agctaatga gcaccggaa 
      cgaatggtt tgaaggtgt gcataaatg taagctcga 
      cgagacgtt gtttacgat tatggacaa gattgtcaa 
      agcagaggt gcataggct cgtggttgt tgacgacga 
      cgacaaagt tataggaat aatatccct gtctgatct 
      attccttta cctggtgtt gcgaccttg cggcgaaga 
      cggttctcc cgacggtgt ggcttcggt gagggcgca 
      agacataaa actgcagga gacgatttc gcagtcgga 
      gaggacgtc ctccactga agaagtgtc gcaaacaat 
      ccccgaaga ggaggaaga cccgaccgt agagaaaaa 
      tactcaaga agaaaaaag cgaaaacca taaagatga 
      ttccgacac gagtctgcc cgattctcc cgtcatcga 
      aagtacgac gtcgttacc gagcgagaa cagcatgtt 
      cagagaggt gacggtgac gggaggcgg tgaataatt 
      ttgttttta ttttctggg tgattagta tttaagatg 
      tgacggttc atgttagtt ttgagccgg tcctggatt 
      gtttctttg ttacgggac gttggtcgt ttgttagtc 
      gacaatgtc tgttacttt gtgatcgct tgtcctaaa 
      tttacaagg gactataat atttttaag aaatttgta 
      cttaataag ttacatacc aaagactgc tttacgtca 
      tgccgagtg acgggtgga agaaacaat tcagatatc 
      aactaactc atggtgtgc tttatttat ttgtataat 
      acactacac ttatttttt gtcagatag tgctgtaaa 
      aagagataa aatggttaa catgttatt gttacaact 
      atatcctat aatgtaaaa ttactcaat ttagcttca 
      cggaacagt gctttgatg acattgaag atgaaagtc 
      gggaggaat ataaattgt ttaaacata gtaacaaag 
      aagccttat atttcgtac aatagagga tttgtgctt 
      aatgttatc aaagcagaa aaacctcaa attaacact 
      tttttgtaa atactgaag aatggtgga ttatatttt 
      taatattgt gtggatttt ttaatttca gagctgaag 
      gaaaagtat cttgatgag atctacaat ttttaaatt 
      ttatatata aaaattaag ataatgtac agttcgaaa 
      taaatgatt taaagtgag gttcactca aagttggta 
      tttgctaat ttattgtta aggtagttt aagatatac 
      aacgatgta gttacataa tatttagtt agctaggct 
      catggccta tacgatgct tagtaaa
    • >TC033217 RC
      tctttgata acaagctta acatgtatt attcatcat 
      tgggttaca tttcaaacg caactgttt tgtttttag 
      gggcgcatt tgaggcaga gatctttag atactgaaa 
      gacggcggc aatctctcg gaactatca ttcctcaca 
      gggcttcag acgctttcc tcgaccctt atcatgctg 
      cgattcttt tcagggatg caagagggt taccagtcg 
      tcgatccgt tcctagaaa aagtcaata tatcggatt 
      taggttcgc ggaacgcac taaaaacga gagtctaaa 
      ttaaagaaa gaaaacatt ccaaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY