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Gene information
General information
TC014667 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13194596..13207080
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014667
    iBeetle screen iB_05670

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014667 RA
      MDSDVSLHP KSVDKVSVE PLHQPLPTM PSKSGSSST 
      ISLSDESDI EPDELVSTI NHNTESSMA ALAGEERDF 
      GSSMFFKRY FQNHSNTLP SRVAVSSRR LSQCREEDE 
      DEEKKDLKE QVPSNSNMS TISGSDKSL SESSSGSKT 
      SVIDTVSGP THKFVITKT KQPSEAAKI FANRLHYRQ 
      ANTVHAIPS HDSKRPSVY NIFRTSLPS PHYDTRFFD 
      SSLIEMKSE NSSSSTIDC TGSAEDIWV KRSPTELKK 
      ELGSQPLPT ITSDDVDTQ SMPRPRSGT WGSKSDSKQ 
      KSKDKSKDG KSKQGKEHK SEGKKEKKK DKHSGTRSG 
      SASRSNSAE RRKSEDVQS PKKAGMLDA FRTRSNSDA 
      SKKKGSAFM ASMKSAMLH TGLMHARPK APRDGSAHP 
      VRDDGSHTQ YYHTVTAAS SNRSPMTKV MDIFRNRSH 
      ASVPPEDRR KKAAQQLSG KYTYFVSRN
    CDS sequence
    • >TC014667 RA
      atggactct gatgtgtcc ctgcaccca aaatcagtg 
      gacaaagtg agtgtggag ccgctccac cagcccctg 
      cctacgatg ccttcgaaa agcggctcg tcgtcgacg 
      atctcgctc tcggacgag agcgacatc gagcccgac 
      gagctggtg tcgacaatc aaccacaac acggagtcc 
      tcgatggcg gccctcgcc ggcgaagag cgcgacttc 
      ggcagctcg atgttcttc aagcgctac ttccagaac 
      cacagcaac acgctgcct tcgcgcgtg gccgtctcg 
      tcgcggcgg ctgtcgcag tgccgcgag gaggacgag 
      gacgaggag aaaaaagac ttgaaagaa caagtgcct 
      tccaacagc aacatgtcg accatctcc gggagcgac 
      aaaagtctg tcggagtcg tcgagcggg tcgaaaacg 
      tctgtgatc gatactgtt agtggacct actcataag 
      ttcgtgatt acaaagact aagcagccc tcggaggcg 
      gcgaagatc ttcgccaat aggctgcac tacaggcag 
      gcgaacaca gtgcacgcg atcccctcg cacgactcc 
      aagaggccc tcggtttac aacatcttc aggacctcg 
      ctgccttcg ccgcattat gataccagg ttttttgac 
      tcgtcgctt attgaaatg aagagtgag aactcgagc 
      agttccacc atcgattgc acgggcagt gcggaggat 
      atttgggtg aaaagatcg cccactgaa ttgaaaaag 
      gaactgggt tcccagccc ctcccgacc atcaccagc 
      gacgatgtc gacacccag tccatgccc aggccccgc 
      tctggaacg tggggctct aaatcagac agcaaacag 
      aagagcaaa gacaaatcg aaagacggt aaaagcaag 
      cagggtaaa gaacacaaa agcgagggc aagaaggag 
      aagaagaag gacaaacat tcgggaact cggagcggg 
      tcagcgtcc aggtcgaat tcggccgaa cggaggaag 
      tccgaagat gtgcaaagt cccaagaaa gcgggaatg 
      ttggacgcc ttcaggacc aggtcgaac tcagacgcg 
      agcaagaag aagggaagt gcttttatg gcgtccatg 
      aagagtgcc atgctgcat acaggtttg atgcatgca 
      cgtccgaag gcaccacgg gatgggtct gcccatcca 
      gtccgtgat gatggttcc cacacccaa tattaccac 
      accgttacc gcagcttct tccaatagg agccccatg 
      accaaagtg atggatatc ttcaggaat cggtcgcac 
      gcaagtgta ccaccggag gatagacga aagaaagcg 
      gcccaacaa ctgtccggt aagtacacg tattttgtg 
      tccagaaat tga
    mRNA sequence
    • >TC014667 RA
      aacatttca tttttcggt tggcagcga tcagctgag 
      cgcgccgcc tgcgtcatc agctggtat taacaaatg 
      actctcgtt ttctcgcga tgactgcct ttgcttatt 
      tctgttagt caccggttt gccttatga gcgcgaact 
      gcattagtg tatggcgaa atggtgttt tacacgtgt 
      tattgcggc cacccccat tcaaaccgt gccgttttt 
      gcccctaga agagtcagc aaagtgcaa ttttgagtg 
      gattttggc tgtgcggca ttcgtacgg tttttcagt 
      gtttgtgat tttttacgt ggatttttt ctggttttt 
      ttacatggt ttggaaaat ctatttcgc tttaatcgg 
      atttgctac ttactgtca gtaagatgg actctgatg 
      tgtccctgc acccaaaat cagtggaca aagtgagtg 
      tggagccgc tccaccagc ccctgccta cgatgcctt 
      cgaaaagcg gctcgtcgt cgacgatct cgctctcgg 
      acgagagcg acatcgagc ccgacgagc tggtgtcga 
      caatcaacc acaacacgg agtcctcga tggcggccc 
      tcgccggcg aagagcgcg acttcggca gctcgatgt 
      tcttcaagc gctacttcc agaaccaca gcaacacgc 
      tgccttcgc gcgtggccg tctcgtcgc ggcggctgt 
      cgcagtgcc gcgaggagg acgaggacg aggagaaaa 
      aagacttga aagaacaag tgccttcca acagcaaca 
      tgtcgacca tctccggga gcgacaaaa gtctgtcgg 
      agtcgtcga gcgggtcga aaacgtctg tgatcgata 
      ctgttagtg gacctactc ataagttcg tgattacaa 
      agactaagc agccctcgg aggcggcga agatcttcg 
      ccaataggc tgcactaca ggcaggcga acacagtgc 
      acgcgatcc cctcgcacg actccaaga ggccctcgg 
      tttacaaca tcttcagga cctcgctgc cttcgccgc 
      attatgata ccaggtttt ttgactcgt cgcttattg 
      aaatgaaga gtgagaact cgagcagtt ccaccatcg 
      attgcacgg gcagtgcgg aggatattt gggtgaaaa 
      gatcgccca ctgaattga aaaaggaac tgggttccc 
      agcccctcc cgaccatca ccagcgacg atgtcgaca 
      cccagtcca tgcccaggc cccgctctg gaacgtggg 
      gctctaaat cagacagca aacagaaga gcaaagaca 
      aatcgaaag acggtaaaa gcaagcagg gtaaagaac 
      acaaaagcg agggcaaga aggagaaga agaaggaca 
      aacattcgg gaactcgga gcgggtcag cgtccaggt 
      cgaattcgg ccgaacgga ggaagtccg aagatgtgc 
      aaagtccca agaaagcgg gaatgttgg acgccttca 
      ggaccaggt cgaactcag acgcgagca agaagaagg 
      gaagtgctt ttatggcgt ccatgaaga gtgccatgc 
      tgcatacag gtttgatgc atgcacgtc cgaaggcac 
      cacgggatg ggtctgccc atccagtcc gtgatgatg 
      gttcccaca cccaatatt accacaccg ttaccgcag 
      cttcttcca ataggagcc ccatgacca aagtgatgg 
      atatcttca ggaatcggt cgcacgcaa gtgtaccac 
      cggaggata gacgaaaga aagcggccc aacaactgt 
      ccggtaagt acacgtatt ttgtgtcca gaaattgat 
      aaaaatgta ttggtggga gttatcaca cacatttcg 
      aaatgttag gtcgctgta gcgcgaatc gaatcattc 
      cggcagatg gcgcgttcg gggtatttt ctcgcatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY