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Gene information
General information
TC014666 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13169046..13173538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014666
    iBeetle screen iB_05669

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3
    Comment: Dramatically decreased.
    egg chamber not present - Number of animals showing the phenotype: 3

    vitellarium potentiallynot present - Number of animals showing the phenotype: 3

    tropharium structure irregular - Number of animals showing the phenotype: 3

    Comment: Anterior parts seem to be more affected.  
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Comment: cannibalized, not fully analyzable

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014666 RA
      MMATAVQKN SLEGLSNGN GLVHPPLNG YGGDVEEVS 
      APEAAAGDS AVEEPEIDI MINNVVCSF SVRCHLNLR 
      EIALNGINV EYRKENGKI TMKLRRPYT TASIWSSGK 
      VTCTGATSE LQAKIAARR FARCLQKLG FKVRFNNYR 
      VVNVLGTCS MPFAIKINS FSERHKEAD YEPELHPGV 
      TYKLQSPKA TLKIFSTGS VTVTAPSVA DVQAAIEYI 
      FPLVYEFRK ERTPEEAAL LALKKSRQM TRSPAYDEE 
      EEYQEEESD WD
    CDS sequence
    • >TC014666 RA
      atgatggcc accgctgtt cagaagaac agcctcgag 
      ggcctctcc aacggcaac ggcctggtg cacccgccc 
      ctcaacggc tacgggggc gacgtggag gaggtcagc 
      gcccccgag gcggcggcc ggcgacagt gcggtggag 
      gagcccgag atagacatc atgataaac aacgtggtg 
      tgcagtttt agtgttcgg tgccacttg aacttgcgc 
      gagatcgct ctgaacggt atcaacgtc gaataccgc 
      aaggaaaac ggcaagatc acgatgaag ctgcgaagg 
      ccctacacc accgccagc atctggtcc tcggggaag 
      gtcacctgc acgggggcc accagcgag ctgcaggcc 
      aagatcgcg gcgaggagg ttcgctcgt tgtttacaa 
      aaactaggg tttaaggtg cgcttcaac aactacaga 
      gtggttaat gtgctcggg acctgctcc atgcccttc 
      gcgatcaag atcaattcc ttctcggaa aggcataaa 
      gaggctgat tatgaacct gaattacat cccggagtc 
      acgtacaaa ctacaaagt cctaaagcc accttaaag 
      atattttcc acaggcagt gttactgtt acagcgccg 
      agtgtggca gacgttcaa gccgccatc gaatacatt 
      ttccctttg gtgtatgag ttccgtaaa gagcgaacc 
      ccggaagag gcggcgctg ctcgctttg aagaagtcg 
      cgccagatg acacgtagt cccgcttac gacgaagaa 
      gaagagtat caagaagaa gaaagtgac tgggactag
    mRNA sequence
    • >TC014666 RA
      atgaatcaa ataataaag agttaaaaa ccatcagcg 
      ccgtcccgt cagtgcgat ttcatctgt agtgagtgt 
      atcatggct tcgcacaat ggcaaaaat ttcattaaa 
      taagatttt cgtcaaaaa tcgtgcaaa agggaccaa 
      atcagcaaa actgaaatt tagcacaaa tcgcagcaa 
      ctcgtgata ggacttcct tcctgtgtc ctaaaaggt 
      ataaactcg tgttgcatt ccgatttct cgtggaaat 
      ttctgtagc gaatcgccc ccacagcac agctcgtgt 
      tttcgggac cttggggac cacccccta gctcgtgtc 
      agatatttt cgttccgtg acatgcaag tcattagcc 
      gtctgttgc agggctggg ggctgtcgg aatgatggc 
      caccgctgt tcagaagaa cagcctcga gggcctctc 
      caacggcaa cggcctggt gcacccgcc cctcaacgg 
      ctacggggg cgacgtgga ggaggtcag cgcccccga 
      ggcggcggc cggcgacag tgcggtgga ggagcccga 
      gatagacat catgataaa caacgtggt gtgcagttt 
      tagtgttcg gtgccactt gaacttgcg cgagatcgc 
      tctgaacgg tatcaacgt cgaataccg caaggaaaa 
      cggcaagat cacgatgaa gctgcgaag gccctacac 
      caccgccag catctggtc ctcggggaa ggtcacctg 
      cacgggggc caccagcga gctgcaggc caagatcgc 
      ggcgaggag gttcgctcg ttgtttaca aaaactagg 
      gtttaaggt gcgcttcaa caactacag agtggttaa 
      tgtgctcgg gacctgctc catgccctt cgcgatcaa 
      gatcaattc cttctcgga aaggcataa agaggctga 
      ttatgaacc tgaattaca tcccggagt cacgtacaa 
      actacaaag tcctaaagc caccttaaa gatattttc 
      cacaggcag tgttactgt tacagcgcc gagtgtggc 
      agacgttca agccgccat cgaatacat tttcccttt 
      ggtgtatga gttccgtaa agagcgaac cccggaaga 
      ggcggcgct gctcgcttt gaagaagtc gcgccagat 
      gacacgtag tcccgctta cgacgaaga agaagagta 
      tcaagaaga agaaagtga ctgggacta gagtgtagt 
      gatggtgtt ctttagcta taatttgtt tagtgactt 
      ttcctcgat ttctacgtt ttcgtgctt atttgacaa 
      ttctttcga gtattcgga tcgtccgca tttatcacg 
      gccaaagaa tgcaaatca aaattctat cgctccaaa 
      ttttataag aaattttaa tcaccgcgg acgtccgaa 
      gtacaatac gaataaccg actgttttt cacactctt 
      gtataaaat aatgtatcg tgtatctga ttatcatta 
      ataatttat ttgatataa ttagataga gctgtagga 
      ctaatcgac tgatgaact acgagtacg agtggcaat 
      aagtgatag tgacccacg atatttcgg tccgcagct 
      tctagagta atgttcatg tagacataa ggcctagca 
      tcgtcaatt tgtacttcg taaagccag tgattgcac 
      ttatttctt tcatcaagt gtagctact gcatccgat 
      ttgtttctc ttgaatata tttttacaa acttgaatt 
      aaacataag tgtcttact tgtatgtgt cgttgtggg 
      ttgctttac ttttgtaca cctgacaaa accaaaaat 
      ttatcgcaa tttggccta tatgacatt tcttctgac 
      gtttgagta tgagtgctt ttacttcat tatgaactc 
      aataatcag tagtcatta ttgtatttg atgtttctg 
      ggtaattaa cactcgtac tataaatcg tgtcgtcaa 
      aataaatgt gattcaaag taaccacac ttcgtgtaa 
      gattgactg taaaagcaa ttaggaact ccctgagcc 
      ttattaata gtaataaaa tttaactaa taaagacaa 
      ctgtaattt cgtatcaga atttgtttc ggttctatt 
      ggcacgtgc tttatggaa attttttgt ttttttagg 
      acgtccgga tctttgttc cttctacat atgtcagaa 
      tatgtgtaa tgttaatgt ataccaaat caataattt 
      agtagttaa ttgatccat gatcatgtt agagatatt 
      aattgcttt cctaagtta aagcttgtt ctcatagtg 
      tgtattata atttttatg cgtatctac tgtattctg 
      aatgtagat ttaataaag ttttctcat tttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY