General information | |||
TC014643 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12745427..12747406 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC014643 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05667 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC014643 RA
MIVTTSAFK MNPKIYKTL YKLFQEYYF RKNVSGDEF IEEKLLAPD SHLTDEEIE KFVLLVTHK KFLEAIKNN FVSFHNLRN YNDVRMIMI IFILLFASD NVAPNELFE VFNEVKCAF KHKILQYFF NENNIIQTA LTGCKFFED EYILNQIIN PLLNKTDVL KKIERLLYQ EKIKQRTPK PPTVPNQMA LLQRKKVAP TVPLNTPAE PKFFQARDV PKTLYQPDT TDKKLVSEF EKNKLKAQK LLAQAKALD KNYCKPKQA LVVEEAPRR IFRAKKAPS RKTNIETRG NVAAVLREA SVCIKEQEK EIKNIEVLL KGGATLDKY RELEENLRQ EEQQKSIEE IERKHLEGL LTFEDAILA KRKLIECNK ERFKEFMEE RDAFLKQLE DWKERQQER IKMTVKKCQ EIERNARDS DRKLKEEKK NLVVQFKEE KRQMLQNAY EKQKEELAK KIKLIEEIK ALHEMKTLH SVTRSLTGQ SVPSSVCCA KCRLAICSK SSPK
- >TC014643 RA
atgatcgtg acaacgtca gccttcaaa atgaacccg aaaatatac aaaactttg tacaaattg ttccaagag tattatttt agaaaaaat gtgagcggt gacgagttc attgaagag aaattacta gcccccgac tcgcaccta acagatgaa gaaatcgaa aagtttgtt ttgcttgtc acccataaa aagttcctg gaagcgatc aaaaataac ttcgtctcg ttccacaat ctccgaaat tacaacgac gttcgaatg atcatgatc atatttatc cttctgttt gcctcagac aatgttgcc cccaatgag ctattcgag gttttcaat gaagtcaaa tgtgccttc aaacacaaa attttgcag tatttcttc aacgaaaac aacatcatt caaactgca ttaaccgga tgcaagttt ttcgaagac gaatatatt ctaaaccaa attattaac ccgctcctt aacaaaacc gacgtcttg aaaaaaata gaaaggtta ctctatcag gaaaaaatc aaacaacgg accccaaaa ccaccaacg gttccgaac caaatggcc ctgttacaa agaaaaaaa gtcgctccg actgtccca ctcaacacc cctgcggaa ccaaaattc tttcaagca agggatgtt cctaaaact ctctaccaa cctgatacc accgacaag aaacttgtg agtgagttt gagaaaaat aaactaaag gcccagaaa ttactagca caagctaaa gctttggat aagaattat tgtaagcct aagcaagcg ttggttgtt gaagaagcc ccaagaaga attttccgg gctaaaaaa gccccttct aggaaaacg aacattgag actagaggt aacgtcgct gccgtttta cgggaagct agtgtttgt ataaaagag caagagaag gaaataaaa aatatcgaa gttttgcta aaaggcggt gcgacttta gacaagtac agagagttg gaggagaat cttcgtcaa gaggagcaa caaaaatca attgaggaa attgagcga aagcatttg gaggggtta ttgactttt gaggacgcc attttggcc aagagaaag ctgattgag tgtaataaa gaacgtttc aaggaattt atggaagag cgggacgcg tttttaaag cagttggaa gactggaag gagagacag caagagaga ataaaaatg acggtaaaa aagtgccaa gaaatcgaa cgaaatgcc agagattcc gacaggaaa ctcaaagaa gagaagaag aatctagtc gttcagttt aaggaagaa aagaggcaa atgttgcaa aatgcgtac gaaaaacag aaggaagag ctggctaag aagatcaaa ctgattgaa gaaatcaaa gcgttgcac gaaatgaag actcttcat tctgttaca aggagtttg acaggacag agtgtccca agctcggtt tgctgtgcg aaatgtcga ttggcgatt tgcagcaaa agctcgccg aaatga
- >TC014643 RA
cgaacgcag ggccggctc tgatgatca catcctggg catccttgt gtctcagat tgaaaacat cagtattgt taactttga taacagaat ccccagcca acaatccag cccgctctg caggggaac caccgttca cctggaaat caaaaatga tcgtgacaa cgtcagcct tcaaaatga acccgaaaa tatacaaaa ctttgtaca aattgttcc aagagtatt attttagaa aaaatgtga gcggtgacg agttcattg aagagaaat tactagccc ccgactcgc acctaacag atgaagaaa tcgaaaagt ttgttttgc ttgtcaccc ataaaaagt tcctggaag cgatcaaaa ataacttcg tctcgttcc acaatctcc gaaattaca acgacgttc gaatgatca tgatcatat ttatccttc tgtttgcct cagacaatg ttgccccca atgagctat tcgaggttt tcaatgaag tcaaatgtg ccttcaaac acaaaattt tgcagtatt tcttcaacg aaaacaaca tcattcaaa ctgcattaa ccggatgca agtttttcg aagacgaat atattctaa accaaatta ttaacccgc tccttaaca aaaccgacg tcttgaaaa aaatagaaa ggttactct atcaggaaa aaatcaaac aacggaccc caaaaccac caacggttc cgaaccaaa tggccctgt tacaaagaa aaaaagtcg ctccgactg tcccactca acacccctg cggaaccaa aattctttc aagcaaggg atgttccta aaactctct accaacctg ataccaccg acaagaaac ttgtgagtg agtttgaga aaaataaac taaaggccc agaaattac tagcacaag ctaaagctt tggataaga attattgta agcctaagc aagcgttgg ttgttgaag aagccccaa gaagaattt tccgggcta aaaaagccc cttctagga aaacgaaca ttgagacta gaggtaacg tcgctgccg ttttacggg aagctagtg tttgtataa aagagcaag agaaggaaa taaaaaata tcgaagttt tgctaaaag gcggtgcga ctttagaca agtacagag agttggagg agaatcttc gtcaagagg agcaacaaa aatcaattg aggaaattg agcgaaagc atttggagg ggttattga cttttgagg acgccattt tggccaaga gaaagctga ttgagtgta ataaagaac gtttcaagg aatttatgg aagagcggg acgcgtttt taaagcagt tggaagact ggaaggaga gacagcaag agagaataa aaatgacgg taaaaaagt gccaagaaa tcgaacgaa atgccagag attccgaca ggaaactca aagaagaga agaagaatc tagtcgttc agtttaagg aagaaaaga ggcaaatgt tgcaaaatg cgtacgaaa aacagaagg aagagctgg ctaagaaga tcaaactga ttgaagaaa tcaaagcgt tgcacgaaa tgaagactc ttcattctg ttacaagga gtttgacag gacagagtg tcccaagct cggtttgct gtgcgaaat gtcgattgg cgatttgca gcaaaagct cgccgaaat gagagacaa aatggagcg tcagttgga gcagaagcg aaagtgtat tcaaaagta caaagagag acagaggct gatgatgga aaacgtgaa gaattttat tacgcaaac tcgaatgtc aaagccgaa acaaacaac tgtgccaaa tcgtccagc aaaattagc acgaagttc tgatctaga taagctgag aggtcagtt ggaggaagc aagggaaat gaggaaaaa ccacgtcca gcttgatgc aaaaccgtg tcctcagca tttacagag ataccgata tactttaaa catatgatt gttaaaaaa attgaaaaa tacacgttt tgtgagtca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
