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Gene information
General information
TC014643 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12745427..12747406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC014643
      iBeetle screen iB_05667

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014643 RA
        MIVTTSAFK MNPKIYKTL YKLFQEYYF RKNVSGDEF 
IEEKLLAPD SHLTDEEIE KFVLLVTHK KFLEAIKNN 
FVSFHNLRN YNDVRMIMI IFILLFASD NVAPNELFE 
VFNEVKCAF KHKILQYFF NENNIIQTA LTGCKFFED 
EYILNQIIN PLLNKTDVL KKIERLLYQ EKIKQRTPK 
PPTVPNQMA LLQRKKVAP TVPLNTPAE PKFFQARDV 
PKTLYQPDT TDKKLVSEF EKNKLKAQK LLAQAKALD 
KNYCKPKQA LVVEEAPRR IFRAKKAPS RKTNIETRG 
NVAAVLREA SVCIKEQEK EIKNIEVLL KGGATLDKY 
RELEENLRQ EEQQKSIEE IERKHLEGL LTFEDAILA 
KRKLIECNK ERFKEFMEE RDAFLKQLE DWKERQQER 
IKMTVKKCQ EIERNARDS DRKLKEEKK NLVVQFKEE 
KRQMLQNAY EKQKEELAK KIKLIEEIK ALHEMKTLH 
SVTRSLTGQ SVPSSVCCA KCRLAICSK SSPK
      CDS sequence
      • >TC014643 RA
        atgatcgtg acaacgtca gccttcaaa atgaacccg 
aaaatatac aaaactttg tacaaattg ttccaagag 
tattatttt agaaaaaat gtgagcggt gacgagttc 
attgaagag aaattacta gcccccgac tcgcaccta 
acagatgaa gaaatcgaa aagtttgtt ttgcttgtc 
acccataaa aagttcctg gaagcgatc aaaaataac 
ttcgtctcg ttccacaat ctccgaaat tacaacgac 
gttcgaatg atcatgatc atatttatc cttctgttt 
gcctcagac aatgttgcc cccaatgag ctattcgag 
gttttcaat gaagtcaaa tgtgccttc aaacacaaa 
attttgcag tatttcttc aacgaaaac aacatcatt 
caaactgca ttaaccgga tgcaagttt ttcgaagac 
gaatatatt ctaaaccaa attattaac ccgctcctt 
aacaaaacc gacgtcttg aaaaaaata gaaaggtta 
ctctatcag gaaaaaatc aaacaacgg accccaaaa 
ccaccaacg gttccgaac caaatggcc ctgttacaa 
agaaaaaaa gtcgctccg actgtccca ctcaacacc 
cctgcggaa ccaaaattc tttcaagca agggatgtt 
cctaaaact ctctaccaa cctgatacc accgacaag 
aaacttgtg agtgagttt gagaaaaat aaactaaag 
gcccagaaa ttactagca caagctaaa gctttggat 
aagaattat tgtaagcct aagcaagcg ttggttgtt 
gaagaagcc ccaagaaga attttccgg gctaaaaaa 
gccccttct aggaaaacg aacattgag actagaggt 
aacgtcgct gccgtttta cgggaagct agtgtttgt 
ataaaagag caagagaag gaaataaaa aatatcgaa 
gttttgcta aaaggcggt gcgacttta gacaagtac 
agagagttg gaggagaat cttcgtcaa gaggagcaa 
caaaaatca attgaggaa attgagcga aagcatttg 
gaggggtta ttgactttt gaggacgcc attttggcc 
aagagaaag ctgattgag tgtaataaa gaacgtttc 
aaggaattt atggaagag cgggacgcg tttttaaag 
cagttggaa gactggaag gagagacag caagagaga 
ataaaaatg acggtaaaa aagtgccaa gaaatcgaa 
cgaaatgcc agagattcc gacaggaaa ctcaaagaa 
gagaagaag aatctagtc gttcagttt aaggaagaa 
aagaggcaa atgttgcaa aatgcgtac gaaaaacag 
aaggaagag ctggctaag aagatcaaa ctgattgaa 
gaaatcaaa gcgttgcac gaaatgaag actcttcat 
tctgttaca aggagtttg acaggacag agtgtccca 
agctcggtt tgctgtgcg aaatgtcga ttggcgatt 
tgcagcaaa agctcgccg aaatga
      mRNA sequence
      • >TC014643 RA
        cgaacgcag ggccggctc tgatgatca catcctggg 
catccttgt gtctcagat tgaaaacat cagtattgt 
taactttga taacagaat ccccagcca acaatccag 
cccgctctg caggggaac caccgttca cctggaaat 
caaaaatga tcgtgacaa cgtcagcct tcaaaatga 
acccgaaaa tatacaaaa ctttgtaca aattgttcc 
aagagtatt attttagaa aaaatgtga gcggtgacg 
agttcattg aagagaaat tactagccc ccgactcgc 
acctaacag atgaagaaa tcgaaaagt ttgttttgc 
ttgtcaccc ataaaaagt tcctggaag cgatcaaaa 
ataacttcg tctcgttcc acaatctcc gaaattaca 
acgacgttc gaatgatca tgatcatat ttatccttc 
tgtttgcct cagacaatg ttgccccca atgagctat 
tcgaggttt tcaatgaag tcaaatgtg ccttcaaac 
acaaaattt tgcagtatt tcttcaacg aaaacaaca 
tcattcaaa ctgcattaa ccggatgca agtttttcg 
aagacgaat atattctaa accaaatta ttaacccgc 
tccttaaca aaaccgacg tcttgaaaa aaatagaaa 
ggttactct atcaggaaa aaatcaaac aacggaccc 
caaaaccac caacggttc cgaaccaaa tggccctgt 
tacaaagaa aaaaagtcg ctccgactg tcccactca 
acacccctg cggaaccaa aattctttc aagcaaggg 
atgttccta aaactctct accaacctg ataccaccg 
acaagaaac ttgtgagtg agtttgaga aaaataaac 
taaaggccc agaaattac tagcacaag ctaaagctt 
tggataaga attattgta agcctaagc aagcgttgg 
ttgttgaag aagccccaa gaagaattt tccgggcta 
aaaaagccc cttctagga aaacgaaca ttgagacta 
gaggtaacg tcgctgccg ttttacggg aagctagtg 
tttgtataa aagagcaag agaaggaaa taaaaaata 
tcgaagttt tgctaaaag gcggtgcga ctttagaca 
agtacagag agttggagg agaatcttc gtcaagagg 
agcaacaaa aatcaattg aggaaattg agcgaaagc 
atttggagg ggttattga cttttgagg acgccattt 
tggccaaga gaaagctga ttgagtgta ataaagaac 
gtttcaagg aatttatgg aagagcggg acgcgtttt 
taaagcagt tggaagact ggaaggaga gacagcaag 
agagaataa aaatgacgg taaaaaagt gccaagaaa 
tcgaacgaa atgccagag attccgaca ggaaactca 
aagaagaga agaagaatc tagtcgttc agtttaagg 
aagaaaaga ggcaaatgt tgcaaaatg cgtacgaaa 
aacagaagg aagagctgg ctaagaaga tcaaactga 
ttgaagaaa tcaaagcgt tgcacgaaa tgaagactc 
ttcattctg ttacaagga gtttgacag gacagagtg 
tcccaagct cggtttgct gtgcgaaat gtcgattgg 
cgatttgca gcaaaagct cgccgaaat gagagacaa 
aatggagcg tcagttgga gcagaagcg aaagtgtat 
tcaaaagta caaagagag acagaggct gatgatgga 
aaacgtgaa gaattttat tacgcaaac tcgaatgtc 
aaagccgaa acaaacaac tgtgccaaa tcgtccagc 
aaaattagc acgaagttc tgatctaga taagctgag 
aggtcagtt ggaggaagc aagggaaat gaggaaaaa 
ccacgtcca gcttgatgc aaaaccgtg tcctcagca 
tttacagag ataccgata tactttaaa catatgatt 
gttaaaaaa attgaaaaa tacacgttt tgtgagtca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY