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Gene information
General information
TC014643 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12745427..12747406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014643
      iBeetle screen iB_05667

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014643 RA
        MIVTTSAFK MNPKIYKTL YKLFQEYYF RKNVSGDEF 
        IEEKLLAPD SHLTDEEIE KFVLLVTHK KFLEAIKNN 
        FVSFHNLRN YNDVRMIMI IFILLFASD NVAPNELFE 
        VFNEVKCAF KHKILQYFF NENNIIQTA LTGCKFFED 
        EYILNQIIN PLLNKTDVL KKIERLLYQ EKIKQRTPK 
        PPTVPNQMA LLQRKKVAP TVPLNTPAE PKFFQARDV 
        PKTLYQPDT TDKKLVSEF EKNKLKAQK LLAQAKALD 
        KNYCKPKQA LVVEEAPRR IFRAKKAPS RKTNIETRG 
        NVAAVLREA SVCIKEQEK EIKNIEVLL KGGATLDKY 
        RELEENLRQ EEQQKSIEE IERKHLEGL LTFEDAILA 
        KRKLIECNK ERFKEFMEE RDAFLKQLE DWKERQQER 
        IKMTVKKCQ EIERNARDS DRKLKEEKK NLVVQFKEE 
        KRQMLQNAY EKQKEELAK KIKLIEEIK ALHEMKTLH 
        SVTRSLTGQ SVPSSVCCA KCRLAICSK SSPK
      CDS sequence
      • >TC014643 RA
        atgatcgtg acaacgtca gccttcaaa atgaacccg 
        aaaatatac aaaactttg tacaaattg ttccaagag 
        tattatttt agaaaaaat gtgagcggt gacgagttc 
        attgaagag aaattacta gcccccgac tcgcaccta 
        acagatgaa gaaatcgaa aagtttgtt ttgcttgtc 
        acccataaa aagttcctg gaagcgatc aaaaataac 
        ttcgtctcg ttccacaat ctccgaaat tacaacgac 
        gttcgaatg atcatgatc atatttatc cttctgttt 
        gcctcagac aatgttgcc cccaatgag ctattcgag 
        gttttcaat gaagtcaaa tgtgccttc aaacacaaa 
        attttgcag tatttcttc aacgaaaac aacatcatt 
        caaactgca ttaaccgga tgcaagttt ttcgaagac 
        gaatatatt ctaaaccaa attattaac ccgctcctt 
        aacaaaacc gacgtcttg aaaaaaata gaaaggtta 
        ctctatcag gaaaaaatc aaacaacgg accccaaaa 
        ccaccaacg gttccgaac caaatggcc ctgttacaa 
        agaaaaaaa gtcgctccg actgtccca ctcaacacc 
        cctgcggaa ccaaaattc tttcaagca agggatgtt 
        cctaaaact ctctaccaa cctgatacc accgacaag 
        aaacttgtg agtgagttt gagaaaaat aaactaaag 
        gcccagaaa ttactagca caagctaaa gctttggat 
        aagaattat tgtaagcct aagcaagcg ttggttgtt 
        gaagaagcc ccaagaaga attttccgg gctaaaaaa 
        gccccttct aggaaaacg aacattgag actagaggt 
        aacgtcgct gccgtttta cgggaagct agtgtttgt 
        ataaaagag caagagaag gaaataaaa aatatcgaa 
        gttttgcta aaaggcggt gcgacttta gacaagtac 
        agagagttg gaggagaat cttcgtcaa gaggagcaa 
        caaaaatca attgaggaa attgagcga aagcatttg 
        gaggggtta ttgactttt gaggacgcc attttggcc 
        aagagaaag ctgattgag tgtaataaa gaacgtttc 
        aaggaattt atggaagag cgggacgcg tttttaaag 
        cagttggaa gactggaag gagagacag caagagaga 
        ataaaaatg acggtaaaa aagtgccaa gaaatcgaa 
        cgaaatgcc agagattcc gacaggaaa ctcaaagaa 
        gagaagaag aatctagtc gttcagttt aaggaagaa 
        aagaggcaa atgttgcaa aatgcgtac gaaaaacag 
        aaggaagag ctggctaag aagatcaaa ctgattgaa 
        gaaatcaaa gcgttgcac gaaatgaag actcttcat 
        tctgttaca aggagtttg acaggacag agtgtccca 
        agctcggtt tgctgtgcg aaatgtcga ttggcgatt 
        tgcagcaaa agctcgccg aaatga
      mRNA sequence
      • >TC014643 RA
        cgaacgcag ggccggctc tgatgatca catcctggg 
        catccttgt gtctcagat tgaaaacat cagtattgt 
        taactttga taacagaat ccccagcca acaatccag 
        cccgctctg caggggaac caccgttca cctggaaat 
        caaaaatga tcgtgacaa cgtcagcct tcaaaatga 
        acccgaaaa tatacaaaa ctttgtaca aattgttcc 
        aagagtatt attttagaa aaaatgtga gcggtgacg 
        agttcattg aagagaaat tactagccc ccgactcgc 
        acctaacag atgaagaaa tcgaaaagt ttgttttgc 
        ttgtcaccc ataaaaagt tcctggaag cgatcaaaa 
        ataacttcg tctcgttcc acaatctcc gaaattaca 
        acgacgttc gaatgatca tgatcatat ttatccttc 
        tgtttgcct cagacaatg ttgccccca atgagctat 
        tcgaggttt tcaatgaag tcaaatgtg ccttcaaac 
        acaaaattt tgcagtatt tcttcaacg aaaacaaca 
        tcattcaaa ctgcattaa ccggatgca agtttttcg 
        aagacgaat atattctaa accaaatta ttaacccgc 
        tccttaaca aaaccgacg tcttgaaaa aaatagaaa 
        ggttactct atcaggaaa aaatcaaac aacggaccc 
        caaaaccac caacggttc cgaaccaaa tggccctgt 
        tacaaagaa aaaaagtcg ctccgactg tcccactca 
        acacccctg cggaaccaa aattctttc aagcaaggg 
        atgttccta aaactctct accaacctg ataccaccg 
        acaagaaac ttgtgagtg agtttgaga aaaataaac 
        taaaggccc agaaattac tagcacaag ctaaagctt 
        tggataaga attattgta agcctaagc aagcgttgg 
        ttgttgaag aagccccaa gaagaattt tccgggcta 
        aaaaagccc cttctagga aaacgaaca ttgagacta 
        gaggtaacg tcgctgccg ttttacggg aagctagtg 
        tttgtataa aagagcaag agaaggaaa taaaaaata 
        tcgaagttt tgctaaaag gcggtgcga ctttagaca 
        agtacagag agttggagg agaatcttc gtcaagagg 
        agcaacaaa aatcaattg aggaaattg agcgaaagc 
        atttggagg ggttattga cttttgagg acgccattt 
        tggccaaga gaaagctga ttgagtgta ataaagaac 
        gtttcaagg aatttatgg aagagcggg acgcgtttt 
        taaagcagt tggaagact ggaaggaga gacagcaag 
        agagaataa aaatgacgg taaaaaagt gccaagaaa 
        tcgaacgaa atgccagag attccgaca ggaaactca 
        aagaagaga agaagaatc tagtcgttc agtttaagg 
        aagaaaaga ggcaaatgt tgcaaaatg cgtacgaaa 
        aacagaagg aagagctgg ctaagaaga tcaaactga 
        ttgaagaaa tcaaagcgt tgcacgaaa tgaagactc 
        ttcattctg ttacaagga gtttgacag gacagagtg 
        tcccaagct cggtttgct gtgcgaaat gtcgattgg 
        cgatttgca gcaaaagct cgccgaaat gagagacaa 
        aatggagcg tcagttgga gcagaagcg aaagtgtat 
        tcaaaagta caaagagag acagaggct gatgatgga 
        aaacgtgaa gaattttat tacgcaaac tcgaatgtc 
        aaagccgaa acaaacaac tgtgccaaa tcgtccagc 
        aaaattagc acgaagttc tgatctaga taagctgag 
        aggtcagtt ggaggaagc aagggaaat gaggaaaaa 
        ccacgtcca gcttgatgc aaaaccgtg tcctcagca 
        tttacagag ataccgata tactttaaa catatgatt 
        gttaaaaaa attgaaaaa tacacgttt tgtgagtca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY