Status
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Gene information
General information
TC014642 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12733447..12738046
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014642
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014642 RA
      MFEECFGDG PFIFRLNES GTGPQLLTQ VCLERGWRE 
      FNSDSGDQW NLWWRTSGF PVSHHRNLY AWQYINHIP 
      KGSAICRKD NLVRYLRCM RKVYGSIYD FSPHGYNLP 
      LEYTKLAAE CSRGGRPFS SKNDPPHDK LLEDKPIWI 
      CKPVAQSQG RGIFLFRKL SELSYDSNT IVQRYIEKP 
      LLIGGYKFD LRLYVCIPS YHPVTIYMY REGLARFGT 
      DKFSLNDLR NPFRHLTNS SINKLGPGY TEMKDRIGS 
      GCKWTLRQL RRYFQQAGI SDWLLWQRI ASLVILTVL 
      SHVNQIPPT VNCFEFFGF DVLIDSSLR PWLLEVNLS 
      PALSNDCDA DRSVKKPML HDMFDLLGL PLYNTGLSV 
      FDIFLDEPN ENNNEEKPK NVAFKSTLS VLNAAGRWR 
      RKHRKMSAK QISSRSSSA VRAKVRTVS ATSPKIVNL 
      PKVNIHLPK TKPNYDLNK NDEEDLRKT GIAKKWGNG 
      RDWNFATSS EGGWVRIWP MTIEQPNKN CFLTVKQMV 
      SKVIKFTKS AKELAKNHP TASECQLNE FLQQEMDMS 
      GEIWIPPV
    CDS sequence
    • >TC014642 RA
      atgttcgag gaatgtttc ggagacggt cctttcata 
      ttcaggctg aacgagagc ggcacagga ccacaactc 
      ctcactcag gtttgtctg gagaggggc tggcgcgag 
      tttaactcg gacagcggc gaccagtgg aacctctgg 
      tggcggact tccgggttt ccggtcagc caccaccgg 
      aatctctac gcctggcag tacataaac catataccc 
      aaaggctct gccatttgc cgaaaagac aatttagtc 
      cgttatttg cgctgcatg cgaaaagtt tacggctcg 
      atttacgat tttagccca catgggtac aacctcccc 
      cttgaatac acgaaacta gcggcagaa tgcagccga 
      ggcgggcgt ccattctcg agcaaaaat gacccgccc 
      catgataaa ctactagaa gataaacca atttggatt 
      tgcaaacct gttgctcaa agtcaagga cgcggcatt 
      ttcctattc aggaaactg agcgaactt agctacgat 
      tccaatacg attgtgcag agatacatc gaaaagccg 
      ctgctgata ggggggtac aagtttgac ttgagactt 
      tacgtctgc atcccttct taccaccct gtgaccata 
      tacatgtac agggagggc ttggcccgc tttggcacc 
      gataagttc agtctgaac gatttacgg aaccctttc 
      cgccatctc accaactca tcaatcaat aaactaggg 
      cctggatat acggaaatg aaggacagg atcgggtca 
      gggtgcaag tggacgctg agacagctc cggaggtac 
      ttccagcaa gcgggaatt tccgactgg ctattgtgg 
      caacgcatt gcgtctctt gttatttta acagtgcta 
      agtcacgtg aaccaaatt ccacccaca gtgaattgt 
      tttgaattt ttcgggttc gacgtgcta attgatagc 
      tcgttgcgg ccgtggcta ctcgaggtg aacctgagt 
      ccggccctt agtaacgac tgtgacgcc gacagatcc 
      gtgaaaaag cctatgctg catgatatg ttcgacctg 
      ctaggtctg cccctttac aacaccggc ctttccgtc 
      tttgacatt ttcctagac gaacccaat gaaaataat 
      aacgaggaa aagccgaaa aatgtggcg ttcaagagc 
      actttatcc gtgctgaac gcggcggga agatggcgc 
      cggaagcac cggaaaatg tccgccaaa caaatcagc 
      tcccgctcc agttcggca gtccgggcc aaagtccgg 
      accgtttca gcaacctct ccgaaaatt gtaaatttg 
      ccaaaagtt aacattcac ttgccaaaa accaagcca 
      aattatgat ctaaacaaa aatgacgag gaggatttg 
      aggaaaact ggaattgcg aagaagtgg ggaaacggt 
      cgcgattgg aatttcgcc acgagtagt gaaggcggt 
      tgggtgcga atttggccc atgaccatt gaacagccc 
      aataaaaat tgcttcctg accgtaaag caaatggtt 
      tcgaaagtg atcaagttt acgaagtcg gcgaaagaa 
      ctggcgaag aatcatccg acggcgtcc gagtgtcaa 
      ttgaacgag ttcctgcag caggagatg gacatgtcc 
      ggggagatt tggatacca ccggtttaa
    mRNA sequence
    • >TC014642 RA
      tgcgtctgg tttggcgtc acgacgctg agagctgaa 
      ttttctagt cagtgaact cgcacacga tagaccgcg 
      tgcttgccc gaattttaa ttttcttta tggatttcc 
      ctacattta ggttaaaag tgtccgaaa ggtcgtgat 
      tattgtgca aaagacagg cgccaaatt cggaataat 
      gttcgagga atgtttcgg agacggtcc tttcatatt 
      caggctgaa cgagagcgg cacaggacc acaactcct 
      cactcaggt ttgtctgga gaggggctg gcgcgagtt 
      taactcgga cagcggcga ccagtggaa cctctggtg 
      gcggacttc cgggtttcc ggtcagcca ccaccggaa 
      tctctacgc ctggcagta cataaacca tatacccaa 
      aggctctgc catttgccg aaaagacaa tttagtccg 
      ttatttgcg ctgcatgcg aaaagttta cggctcgat 
      ttacgattt tagcccaca tgggtacaa cctccccct 
      tgaatacac gaaactagc ggcagaatg cagccgagg 
      cgggcgtcc attctcgag caaaaatga cccgcccca 
      tgataaact actagaaga taaaccaat ttggatttg 
      caaacctgt tgctcaaag tcaaggacg cggcatttt 
      cctattcag gaaactgag cgaacttag ctacgattc 
      caatacgat tgtgcagag atacatcga aaagccgct 
      gctgatagg ggggtacaa gtttgactt gagacttta 
      cgtctgcat cccttctta ccaccctgt gaccatata 
      catgtacag ggagggctt ggcccgctt tggcaccga 
      taagttcag tctgaacga tttacggaa ccctttccg 
      ccatctcac caactcatc aatcaataa actagggcc 
      tggatatac ggaaatgaa ggacaggat cgggtcagg 
      gtgcaagtg gacgctgag acagctccg gaggtactt 
      ccagcaagc gggaatttc cgactggct attgtggca 
      acgcattgc gtctcttgt tattttaac agtgctaag 
      tcacgtgaa ccaaattcc acccacagt gaattgttt 
      tgaattttt cgggttcga cgtgctaat tgatagctc 
      gttgcggcc gtggctact cgaggtgaa cctgagtcc 
      ggcccttag taacgactg tgacgccga cagatccgt 
      gaaaaagcc tatgctgca tgatatgtt cgacctgct 
      aggtctgcc cctttacaa caccggcct ttccgtctt 
      tgacatttt cctagacga acccaatga aaataataa 
      cgaggaaaa gccgaaaaa tgtggcgtt caagagcac 
      tttatccgt gctgaacgc ggcgggaag atggcgccg 
      gaagcaccg gaaaatgtc cgccaaaca aatcagctc 
      ccgctccag ttcggcagt ccgggccaa agtccggac 
      cgtttcagc aacctctcc gaaaattgt aaatttgcc 
      aaaagttaa cattcactt gccaaaaac caagccaaa 
      ttatgatct aaacaaaaa tgacgagga ggatttgag 
      gaaaactgg aattgcgaa gaagtgggg aaacggtcg 
      cgattggaa tttcgccac gagtagtga aggcggttg 
      ggtgcgaat ttggcccat gaccattga acagcccaa 
      taaaaattg cttcctgac cgtaaagca aatggtttc 
      gaaagtgat caagtttac gaagtcggc gaaagaact 
      ggcgaagaa tcatccgac ggcgtccga gtgtcaatt 
      gaacgagtt cctgcagca ggagatgga catgtccgg 
      ggagatttg gataccacc ggtttaatt cattattaa 
      aaattgctg atttttcct ctttgctaa attttctct 
      tgttgactt tatcactgc ctcgaaatt tgtcttcaa 
      taaacttgt ccgaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY