Status
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Gene information
General information
TC014632 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12639007..12640615
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014632
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized -
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence -

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014632 RA
      MSQGTQLKR SQKTEFSPI VTRERSFVR PNKDPQKRR 
      SHTMATIKG KPTMEIYRP PNVRTDIPN SGGKLNVHA 
      KEFTMSPHE LQPSKSSGN LAGNFDAIQ LQHSKSSGN 
      ILRHPTLIP IISTPLPLP LLHSTSSSH ILNLPRVNY 
      QFGNEILAH SIFAPKQTP VQTNWNNYQ QSNNLANLK 
      RSKSLGASD SHNLAAKLK EMAKEDIDV GIFPSEHQA 
      NLKLAMEDP NQISSRVMM DLVKVIMEQ VVEGMRFCE 
      AGAKLCVAI IEKEQNQTF LESLLNMCQ QWYQERDKI 
      LRGVKAGDG TRFTAFMWF LTEMCGQLK RHHIREQYE 
      ALQPDLVLL SVLAKCCQA CVSLPIKCL AETECLFFV 
      LTSIGRDLE TELPQQLEQ LLASVRDGF LASAGAPAV 
      RRTLLQLIE LHASNWQLP GSSVLYYYP RIK
    CDS sequence
    • >TC014632 RA
      atgtcgcaa ggaacgcag ttaaaacgc agccaaaag 
      acggagttt tcgccgatt gtgacccgc gaacgcagc 
      ttcgttcgc ccaaataaa gacccgcag aagaggcgc 
      agccacacg atggccacc atcaaaggc aagcccacg 
      atggaaatc tacaggccg ccgaacgtg cggaccgac 
      attcccaac tcgggggga aaactgaac gtgcacgcg 
      aaggagttc acaatgagc ccgcacgag ctgcagccg 
      tcgaagtcg agcgggaac ctggccggg aacttcgac 
      gccatccag ctgcagcat tcgaaatcc agcgggaac 
      atcctgagg caccccacc ttgatcccc atcatttcc 
      acgcccttg cctctccct ctgctccac tcgacgtcc 
      tcctcgcac attttgaac ctcccgagg gttaactac 
      caattcggg aacgaaatt ctggctcat agtatcttc 
      gctccgaag caaactccc gtccagacc aactggaat 
      aactaccag caaagcaac aatctggcc aatttgaag 
      cgttccaaa agtctaggc gcctcagat tcgcacaat 
      ttggccgcg aaactcaaa gaaatggcg aaggaagac 
      atcgacgtt gggattttc cccagcgag caccaagct 
      aacttgaaa ctagccatg gaggacccc aaccagatc 
      tcctcccgg gtcatgatg gacctggtg aaagtgata 
      atggagcaa gtggtggaa gggatgcgg ttttgcgaa 
      gctggggcc aagctgtgt gtggccatc atcgaaaag 
      gagcaaaac cagacgttc ctggaatcc ctgctgaac 
      atgtgccag cagtggtac caggagcgg gacaaaatc 
      ctgcggggg gtgaaggcc ggagacggc accaggttc 
      acagcgttc atgtggttc ctgacggag atgtgcggc 
      cagctgaag cggcaccac atccgggag cagtacgag 
      gcgctccag cccgacctg gtgctgctc tcggtgctg 
      gccaagtgc tgccaggcg tgcgtcagt ttgcccatc 
      aagtgtctg gcggagacg gagtgcctg ttctttgtg 
      ctgacgtcc atcgggagg gatctggag acggagttg 
      ccccagcag ctggagcag ctgctggca agtgtgagg 
      gatgggttt ctggccagt gctggggcg ccggctgtc 
      agacgcacg cttctgcag ctgattgag ttgcatgcg 
      tccaattgg cagctgccg ggatcgtcg gttctgtat 
      tattatccc aggataaag tga
    mRNA sequence
    • >TC014632 RA
      cagcatcaa tcgaataaa atttcctca agccccaaa 
      gccctcaaa atcagcgct cgcacaacc gatttcccc 
      gatcgaaac catgcaacg tcaagtgtg cgccccaac 
      cttgcttta ccaaaattg tgtttagtg cgtgtgcga 
      aatgtcgca aggaacgca gttaaaacg cagccaaaa 
      gacggagtt ttcgccgat tgtgacccg cgaacgcag 
      cttcgttcg cccaaataa agacccgca gaagaggcg 
      cagccacac gatggccac catcaaagg caagcccac 
      gatggaaat ctacaggcc gccgaacgt gcggaccga 
      cattcccaa ctcgggggg aaaactgaa cgtgcacgc 
      gaaggagtt cacaatgag cccgcacga gctgcagcc 
      gtcgaagtc gagcgggaa cctggccgg gaacttcga 
      cgccatcca gctgcagca ttcgaaatc cagcgggaa 
      catcctgag gcaccccac cttgatccc catcatttc 
      cacgccctt gcctctccc tctgctcca ctcgacgtc 
      ctcctcgca cattttgaa cctcccgag ggttaacta 
      ccaattcgg gaacgaaat tctggctca tagtatctt 
      cgctccgaa gcaaactcc cgtccagac caactggaa 
      taactacca gcaaagcaa caatctggc caatttgaa 
      gcgttccaa aagtctagg cgcctcaga ttcgcacaa 
      tttggccgc gaaactcaa agaaatggc gaaggaaga 
      catcgacgt tgggatttt ccccagcga gcaccaagc 
      taacttgaa actagccat ggaggaccc caaccagat 
      ctcctcccg ggtcatgat ggacctggt gaaagtgat 
      aatggagca agtggtgga agggatgcg gttttgcga 
      agctggggc caagctgtg tgtggccat catcgaaaa 
      ggagcaaaa ccagacgtt cctggaatc cctgctgaa 
      catgtgcca gcagtggta ccaggagcg ggacaaaat 
      cctgcgggg ggtgaaggc cggagacgg caccaggtt 
      cacagcgtt catgtggtt cctgacgga gatgtgcgg 
      ccagctgaa gcggcacca catccggga gcagtacga 
      ggcgctcca gcccgacct ggtgctgct ctcggtgct 
      ggccaagtg ctgccaggc gtgcgtcag tttgcccat 
      caagtgtct ggcggagac ggagtgcct gttctttgt 
      gctgacgtc catcgggag ggatctgga gacggagtt 
      gccccagca gctggagca gctgctggc aagtgtgag 
      ggatgggtt tctggccag tgctggggc gccggctgt 
      cagacgcac gcttctgca gctgattga gttgcatgc 
      gtccaattg gcagctgcc gggatcgtc ggttctgta 
      ttattatcc caggataaa gtgaaggag gaaattggc 
      gggacgtgg accaagaga ttgtgtgcg tgtgtgtgt 
      gtggaggtg aaagtttta tttatgtgc tgtaacatt 
      tattcacaa aacactgta agaattttt aacaataaa 
      attttacaa accac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY