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Gene information
General information
TC014627 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12586757..12591805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014627
    iBeetle screen iB_05659

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014627 RA
      MSVECSLST LILAVFGCI VIFLFLLNT LWGLMQATR 
      AILAPFFLP QEETSLIKK YGQWALITG STDGIGKAY 
      AHELAKRGL NIVLVSRST QKLNSVAKE LETEYSIKT 
      KIISADFSL GAQAIKIIK QELGVLDIG ILVNNVGKQ 
      YDYPMYLGE VPERDLWDI ININVGAVT LLCRLFVED 
      MKRRGRGAI VNVSSGSEL QPLPLMTVY AATKAYIKS 
      FTAALRYEY AKHGLTIQH LSPMFINTK MNNFSQTLR 
      ESSTFIPDA STYARHAVS TLGKMDEST GYWAHGIQY 
      FFTSIPPVW IRMYIGGYM NKIFRNEYF TIKNKM
    CDS sequence
    • >TC014627 RA
      atgtcagta gaatgcagc ttgtccact ctgattttg 
      gctgtgttt ggatgtatt gtgattttc ttatttttg 
      ttaaacaca ctatgggga ctgatgcaa gcaactcgt 
      gccatatta gctccgttc tttttaccc caggaagaa 
      acatcactg attaagaaa tacggtcaa tgggcatta 
      attacaggc agcacagac ggaattggc aaagcgtac 
      gctcatgaa cttgccaaa agaggactg aacatagtt 
      ttagtgagt cgaagcacg cagaaatta aacagtgtc 
      gccaaggaa ttagagact gaatattcg attaaaacc 
      aagattatt tccgctgat ttttctctg ggggcgcaa 
      gcaatcaaa ataattaaa caagaactg ggcgtatta 
      gacatcgga atattagta aacaatgtg gggaagcag 
      tacgactac cccatgtac ttgggggaa gtccccgag 
      cgcgacctg tgggacatc atcaacata aacgtgggg 
      gctgtgacc ctgctatgc cgtctgttc gttgaagac 
      atgaagagg agaggtcgt ggagcaatc gtgaacgta 
      tcgtccggg tctgagctc caaccccta cctctaatg 
      accgtttac gcagcaaca aaagcctac ataaagagc 
      ttcacggct gctcttcga tacgaatac gccaaacac 
      ggccttacg atccaacac ctctcacct atgtttata 
      aacaccaaa atgaacaat ttcagccaa acattgcga 
      gaatccagc actttcatc ccagatgcc agcacttat 
      gcaagacat gctgtcagc accttgggg aaaatggac 
      gagtcgacg ggttactgg gcccacgga atacaatac 
      tttttcacg agtattccc ccggtgtgg atacggatg 
      tacatcggt ggttacatg aataagatc ttcaggaat 
      gaatatttt acaataaaa aacaaaatg taa
    mRNA sequence
    • >TC014627 RA
      attgtcgca tttactttc atgataata cgagccgct 
      tttaattga ctcaaacac ccgattggt gaaatatgt 
      aattgaccc ggcattgtg ttcgaaatt attctagtc 
      gcattgttt tatggattt gcaatgtca gtagaatgc 
      agcttgtcc actctgatt ttggctgtg tttggatgt 
      attgtgatt ttcttattt ttgttaaac acactatgg 
      ggactgatg caagcaact cgtgccata ttagctccg 
      ttcttttta ccccaggaa gaaacatca ctgattaag 
      aaatacggt caatgggca ttaattaca ggcagcaca 
      gacggaatt ggcaaagcg tacgctcat gaacttgcc 
      aaaagagga ctgaacata gttttagtg agtcgaagc 
      acgcagaaa ttaaacagt gtcgccaag gaattagag 
      actgaatat tcgattaaa accaagatt atttccgct 
      gatttttct ctgggggcg caagcaatc aaaataatt 
      aaacaagaa ctgggcgta ttagacatc ggaatatta 
      gtaaacaat gtggggaag cagtacgac taccccatg 
      tacttgggg gaagtcccc gagcgcgac ctgtgggac 
      atcatcaac ataaacgtg ggggctgtg accctgcta 
      tgccgtctg ttcgttgaa gacatgaag aggagaggt 
      cgtggagca atcgtgaac gtatcgtcc gggtctgag 
      ctccaaccc ctacctcta atgaccgtt tacgcagca 
      acaaaagcc tacataaag agcttcacg gctgctctt 
      cgatacgaa tacgccaaa cacggcctt acgatccaa 
      cacctctca cctatgttt ataaacacc aaaatgaac 
      aatttcagc caaacattg cgagaatcc agcactttc 
      atcccagat gccagcact tatgcaaga catgctgtc 
      agcaccttg gggaaaatg gacgagtcg acgggttac 
      tgggcccac ggaatacaa tactttttc acgagtatt 
      cccccggtg tggatacgg atgtacatc ggtggttac 
      atgaataag atcttcagg aatgaatat tttacaata 
      aaaaacaaa atgtaattt gaaaaaagt tctttattt 
      tcgtgttat ttgtgttac aaacaatta acttaatta 
      aaactgagt taaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY