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Gene information
General information
TC014625 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12572237..12574592
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014625
    iBeetle screen iB_05658

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    head slightly orientation irregular - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014625 RA
      MTDRSDRRS SLSSNLDEP PNSRLFIIG SKLLCEDDF 
      RKAFEEFGN IEEIWMVRD RQTGESKGV TYIKYSKTS 
      EAARALEAM NGKCIGKLN RSIRVMIAA SRDQGSKRD 
      SNEEERTLR LFVVVPKSM TDSELYDTF KEFGEIDYA 
      TIMRDKESR ESKGFAYVK YFKFSHAAA AFETCDKKY 
      KAVFAEPRK PNMKTDEKF SNSWSSLNK INSSALPLP 
      TVSNPVGYT KLIAIVSPQ VNQDQLWKL FDIVPGLDY 
      CHLRLEGRP KPNKGVASV VYNSAQWAA YAKEKLHGF 
      EYPPGNRII VKPDHEEGS RSTSSERQK HSDIFQIAE 
      TIAQASNLI QAAGLSPDV LQARLGLIP AKDSGINCS 
      VKLPEPQPL APNEETVSR CFIVCTPNP PPPSVLKDV 
      FSRFGNLID VYMLNNRNC GFAKYASTE SAENAIKTL 
      HGAEVCGIR IKVLKAEEK QEHNRKRQR VEEKP
    CDS sequence
    • >TC014625 RA
      atgaccgac agatctgat cgaagatct tcactaagc 
      tctaatcta gacgaacct ccgaactca cgattgttt 
      ataatcgga agcaaactt ctctgtgaa gatgacttc 
      cgcaaagct ttcgaagag tttggaaat atcgaagag 
      atctggatg gttagagac cgacaaact ggtgaaagc 
      aaaggtgtt acgtatatt aaatactcg aaaacttct 
      gaagctgcc cgagctttg gaagccatg aatgggaaa 
      tgtatcggt aaattgaac aggagcatc cgagtcatg 
      atagctgcg agtcgcgac cagggctca aagcgcgac 
      tccaacgag gaggagcgc accctccgc ctctttgtc 
      gtggtcccg aagtccatg accgactcc gaactgtac 
      gacaccttc aaagagttc ggcgaaatc gactacgcc 
      accataatg cgagacaag gaatcgcgc gagagcaag 
      ggcttcgcc tacgtaaaa tacttcaag ttttcgcac 
      gcagctgcc gctttcgaa acgtgcgac aaaaagtac 
      aaagcggtt ttcgccgaa ccccggaaa cctaacatg 
      aaaaccgac gagaaattc tcgaattcc tggagctcg 
      cttaacaaa atcaactca agtgccttg cccttgccc 
      acggtcagc aacccggtc ggttacacg aaactgata 
      gcaatcgtg agcccacaa gtcaaccag gaccagttg 
      tggaagttg tttgatatt gtgccaggg ctggactac 
      tgccatttg cggctggaa gggcgcccc aaacccaac 
      aagggcgtc gcttcggtg gtgtacaac agcgcgcaa 
      tgggcggcc tatgccaag gagaagctc cacgggttt 
      gaatatccc ccagggaac aggattatt gtgaaaccg 
      gaccatgaa gaggggagc aggtctacg tcctcggag 
      agacagaaa cattccgat attttccaa attgcggaa 
      actatcgcc caagcttcg aatttgatt caagcagcg 
      gggctttca cccgacgtt ttgcaggca agattaggg 
      ttaattccc gcgaaagac agtgggatt aactgtagt 
      gttaaacta ccagaaccc cagcctttg gccccgaat 
      gaggaaacg gtgtccaga tgttttatt gtctgtacg 
      cctaacccg cctccacct tccgttctc aaagacgtg 
      ttttcacgt tttgggaac ttaatcgat gtctacatg 
      ttgaataat aggaactgt gggtttgcc aaatacgca 
      agtacagaa tcagccgaa aatgcaata aagactttg 
      cacggggct gaagtttgt ggtatacga atcaaggtt 
      ttgaaagcg gaagaaaaa caagaacat aatcggaaa 
      cgacaaaga gtggaagaa aagccatag
    mRNA sequence
    • >TC014625 RA
      cgttcgacg tcaaacctc tcaaacagc tgctcatct 
      gctgctcct ggtggtgtt gacaatgtg tgaacactg 
      ttgcgtagt tcctttact tttttttct actaacagt 
      actctaaaa tttatatga cacgtcctt aatagtgca 
      acaggttgt cctgtttag gtaaaatga ccgacagat 
      ctgatcgaa gatcttcac taagctcta atctagacg 
      aacctccga actcacgat tgtttataa tcggaagca 
      aacttctct gtgaagatg acttccgca aagctttcg 
      aagagtttg gaaatatcg aagagatct ggatggtta 
      gagaccgac aaactggtg aaagcaaag gtgttacgt 
      atattaaat actcgaaaa cttctgaag ctgcccgag 
      ctttggaag ccatgaatg ggaaatgta tcggtaaat 
      tgaacagga gcatccgag tcatgatag ctgcgagtc 
      gcgaccagg gctcaaagc gcgactcca acgaggagg 
      agcgcaccc tccgcctct ttgtcgtgg tcccgaagt 
      ccatgaccg actccgaac tgtacgaca ccttcaaag 
      agttcggcg aaatcgact acgccacca taatgcgag 
      acaaggaat cgcgcgaga gcaagggct tcgcctacg 
      taaaatact tcaagtttt cgcacgcag ctgccgctt 
      tcgaaacgt gcgacaaaa agtacaaag cggttttcg 
      ccgaacccc ggaaaccta acatgaaaa ccgacgaga 
      aattctcga attcctgga gctcgctta acaaaatca 
      actcaagtg ccttgccct tgcccacgg tcagcaacc 
      cggtcggtt acacgaaac tgatagcaa tcgtgagcc 
      cacaagtca accaggacc agttgtgga agttgtttg 
      atattgtgc cagggctgg actactgcc atttgcggc 
      tggaagggc gccccaaac ccaacaagg gcgtcgctt 
      cggtggtgt acaacagcg cgcaatggg cggcctatg 
      ccaaggaga agctccacg ggtttgaat atcccccag 
      ggaacagga ttattgtga aaccggacc atgaagagg 
      ggagcaggt ctacgtcct cggagagac agaaacatt 
      ccgatattt tccaaattg cggaaacta tcgcccaag 
      cttcgaatt tgattcaag cagcggggc tttcacccg 
      acgttttgc aggcaagat tagggttaa ttcccgcga 
      aagacagtg ggattaact gtagtgtta aactaccag 
      aaccccagc ctttggccc cgaatgagg aaacggtgt 
      ccagatgtt ttattgtct gtacgccta acccgcctc 
      caccttccg ttctcaaag acgtgtttt cacgttttg 
      ggaacttaa tcgatgtct acatgttga ataatagga 
      actgtgggt ttgccaaat acgcaagta cagaatcag 
      ccgaaaatg caataaaga ctttgcacg gggctgaag 
      tttgtggta tacgaatca aggttttga aagcggaag 
      aaaaacaag aacataatc ggaaacgac aaagagtgg 
      aagaaaagc catagaata atctaagta agttcctaa 
      ctgattgtg atatttgta ccaaagaca ataaaaaga 
      aaatagtga taatttatc ctttgtttc aacagtttt 
      tgtgacatg gacgagacg gacgtggaa ctcgtaacg 
      tttgacaac aactttacc gtttcagtg acatggact 
      taacatttt tcaaaagta gctaagcat caattacgt 
      ctgattttc attgtgtat atttttgat acttgcagc 
      aaactattt cattttata aagtcaggt gtaattgat 
      atttctctc ccatcctat acttctcga cattaattt 
      acacaaacc tattatatt ttcgtaaaa aatttttgt 
      atgtatttc cttagtgtg ataatatat tttcaaatt 
      cgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY