Status
Please wait ...
Gene information
General information
TC014620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12498931..12508128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014620
    iBeetle screen iB_05657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014620 RA
      MTSHTNRSN LSLPAQQMP PDAEFDATW AEEDDPHIH 
KLWAIHRTI HTLRGKMSK QQLDASDVM SDEFDENTS 
LPPTYIVIK IASDIKENT LTWLIDKIR GKRRDGGAE 
LIVMRQPSD PDDGWILHL SASKIKFLE TAEEMELMK 
EDKSGMMRE FTVGQLEDF LPEGMHVDD LLTLAEKQT 
IVRHELENI RALAEDEHI PGYPTYTLY EGQSIFQVC 
LKYKIIMKV YPLHDQEAL KKLGRKWYM SLLGKQPIE 
EIRLYFGEA IALYFTFLG FYTATLVVP VFVGLLQLM 
IYSETMSMF CVFNVVWVT LVLEIWKRK SNELAFKWG 
TISMTSLDE PRPNFRGSM GYDAITGKL QPQSPRYLT 
YLKMYCVSI PIVFLCLVA AFVMMLASF WLEEYFKQT 
RTSDDLIIL LPSIVYSIL VYIINCYYR KLATFLTEW 
ENHRTQSQH DRHRVTKLV LFEFVNNFM SLFYIAFVV 
RDMEMLRSQ LQTMLIIFQ LISHVQEAV LPLAVKYYG 
SKFAAWKKQ LFSSNGRSK SRFYKVPTE DQLVPVLRS 
LPQIPIDDY RLDSANKEG EMEDYEGTY DDYLELFIQ 
FGYIFLFSP VYPVAAFWA LFNNILEIR ADAFKLCKI 
YQRPMSRRV KDIGAWQRC FEIIGAMSI MTNCGLLCL 
SPELKRSAP EVGPVEWVL FFVFLEHVL LGIRYLLHI 
TISDKPEWV RVALAKKNY ESKQALKFE RSQKNKGLL 
RRKFKTIHG THAR
    CDS sequence
    • >TC014620 RA
      atgacatcc cacactaat cgctcgaat ctgtcactg 
ccggcgcaa caaatgcct ccagacgcg gaattcgat 
gcgacttgg gccgaagag gacgacccc cacatacac 
aaactgtgg gcgatacac agaacgatc cacacgctg 
agaggtaaa atgagtaaa caacaattg gacgcttca 
gacgtgatg tcggacgaa ttcgacgaa aacacctcc 
ctacctccc acttatatc gtcataaaa atcgcctcg 
gacatcaaa gaaaacacc ctaacgtgg ctgattgac 
aagattaga ggtaagcgt cgcgacggc ggcgccgaa 
ctcatcgtc atgcgacaa ccgagcgat cccgacgat 
ggttggatt ttgcacttg tcggcgtct aagatcaag 
ttcctcgag acggccgaa gagatggag ctgatgaaa 
gaggacaag agcggaatg atgcgtgag ttcaccgtg 
ggccaactg gaggatttc ctccctgaa ggaatgcac 
gtggacgat ttgttaacc cttgccgag aagcagacg 
atcgtccgc cacgaactg gaaaatatc cgagcattg 
gccgaggac gagcacatt cccggctac cccacgtac 
acactatac gagggccaa tcgatattt caagtgtgt 
ctcaagtac aaaataata atgaaggtt tatccgttg 
cacgatcaa gaggcgctg aagaagctg gggcggaag 
tggtacatg tcgttattg ggcaagcag ccgatcgaa 
gaaatccgg ttgtacttt ggtgaagct atagccttg 
tattttacg tttttgggc ttctatact gcaactttg 
gtggtgccc gtcttcgtg gggctcctc cagctgatg 
atctactcg gagacgatg tctatgttt tgtgtcttc 
aatgtggta tgggttaca cttgtctta gagatatgg 
aagagaaaa agcaacgag ctggcgttt aaatggggc 
accatcagc atgacgagt ctggacgag cccagaccc 
aacttccgg ggctcgatg ggctacgac gccatcacg 
gggaagctc cagccacag agccccaga tatctcacc 
tatctcaag atgtattgt gtgtcgatt cccatcgtg 
tttctgtgt cttgtggcg gctttcgtc atgatgttg 
gcctcgttt tggctcgag gagtatttc aaacagacg 
cggacttcc gacgactta atcatactt ctgccgagt 
atagtgtac tcgattcta gtctacata atcaactgt 
tattaccga aaattggcc acttttctg actgaatgg 
gaaaaccac cggactcag tcccaacac gaccgacat 
agagtgaca aagctggta ctttttgaa ttcgtcaac 
aacttcatg tcgctgttt tacattgcg ttcgtcgtg 
cgggacatg gaaatgttg cggagccaa ctccagaca 
atgctgatc atatttcag ttgatcagt cacgtccag 
gaggctgtc ctgccactt gctgttaaa tattacggg 
tctaaattc gccgcgtgg aaaaagcaa cttttctcg 
tcaaatggt cgctcaaaa tccaggttt tacaaagtc 
ccgactgaa gaccaacta gttccagtg ctaaggtcc 
cttccccaa atccccatc gatgattac cgtctggac 
tcggcaaac aaagaggga gagatggag gattacgag 
gggacgtat gatgactac ctcgagctt ttcatccag 
ttcggttac attttcctt ttctcgcct gtgtacccg 
gtcgcggcc ttttgggcg ctttttaac aacattttg 
gagatccga gccgatgcg ttcaaactt tgcaagatc 
taccagcgg ccgatgagt cggcgggtg aaagacata 
ggcgcttgg cagcgctgt ttcgagata atcggcgcc 
atgtcgata atgaccaac tgcggcctt ttgtgcctc 
agtccggag ttgaaaagg tcagccccg gaagtgggc 
ccggtggag tgggtgctg tttttcgtg tttctggag 
cacgttctg ctcggcatt cgatatctg ctgcacatt 
accatttcg gataagccg gaatgggtg cgagtggcc 
ctagctaag aagaattac gagtcgaaa caagccctc 
aaattcgag aggtcgcag aaaaacaaa ggcctgctg 
aggagaaag ttcaaaacg atacacggg acacacgcc 
cgttaa
    mRNA sequence
    • >TC014620 RA
      ttaaattta cgatttgtt tacacaact gcgactttt 
tccacaata aaataaatt cctcgaact tctcaaccc 
ccacgtcca aaatcctgt tgaacaaat ctgaaacgg 
atcagttac cccacattt ggcccaaca acaattacc 
caaagcgct caaatagac aagcagcga acacatgat 
aacaaaata agagaagag gaaaacgag ccaatggcg 
cagactgaa ccactctga aagctccat ccccaacga 
aaaatttcg gtgacgttc cggtgacgc ctgaaaatc 
ccgctgcta agctacacc gtttgagtt ttgtcaaat 
ttttaaagc tcgcaccat ctcagcgcg agtctccgc 
agcaggaat ccgtccact gcaggaaac tagcagctc 
cggcacgtg gcgccctcc cggacccga tccaggtca 
gcactcttt ggttaggaa ttacaagtg cagctccca 
aatttcgaa ttttgcctc aagtatgtc gtaattcga 
atagttgca atcccgatc ctcgagcat gacatccca 
cactaatcg ctcgaatct gtcactgcc ggcgcaaca 
aatgcctcc agacgcgga attcgatgc gacttgggc 
cgaagagga cgaccccca catacacaa actgtgggc 
gatacacag aacgatcca cacgctgag aggtaaaat 
gagtaaaca acaattgga cgcttcaga cgtgatgtc 
ggacgaatt cgacgaaaa cacctccct acctcccac 
ttatatcgt cataaaaat cgcctcgga catcaaaga 
aaacaccct aacgtggct gattgacaa gattagagg 
taagcgtcg cgacggcgg cgccgaact catcgtcat 
gcgacaacc gagcgatcc cgacgatgg ttggatttt 
gcacttgtc ggcgtctaa gatcaagtt cctcgagac 
ggccgaaga gatggagct gatgaaaga ggacaagag 
cggaatgat gcgtgagtt caccgtggg ccaactgga 
ggatttcct ccctgaagg aatgcacgt ggacgattt 
gttaaccct tgccgagaa gcagacgat cgtccgcca 
cgaactgga aaatatccg agcattggc cgaggacga 
gcacattcc cggctaccc cacgtacac actatacga 
gggccaatc gatatttca agtgtgtct caagtacaa 
aataataat gaaggttta tccgttgca cgatcaaga 
ggcgctgaa gaagctggg gcggaagtg gtacatgtc 
gttattggg caagcagcc gatcgaaga aatccggtt 
gtactttgg tgaagctat agccttgta ttttacgtt 
tttgggctt ctatactgc aactttggt ggtgcccgt 
cttcgtggg gctcctcca gctgatgat ctactcgga 
gacgatgtc tatgttttg tgtcttcaa tgtggtatg 
ggttacact tgtcttaga gatatggaa gagaaaaag 
caacgagct ggcgtttaa atggggcac catcagcat 
gacgagtct ggacgagcc cagacccaa cttccgggg 
ctcgatggg ctacgacgc catcacggg gaagctcca 
gccacagag ccccagata tctcaccta tctcaagat 
gtattgtgt gtcgattcc catcgtgtt tctgtgtct 
tgtggcggc tttcgtcat gatgttggc ctcgttttg 
gctcgagga gtatttcaa acagacgcg gacttccga 
cgacttaat catacttct gccgagtat agtgtactc 
gattctagt ctacataat caactgtta ttaccgaaa 
attggccac ttttctgac tgaatggga aaaccaccg 
gactcagtc ccaacacga ccgacatag agtgacaaa 
gctggtact ttttgaatt cgtcaacaa cttcatgtc 
gctgtttta cattgcgtt cgtcgtgcg ggacatgga 
aatgttgcg gagccaact ccagacaat gctgatcat 
atttcagtt gatcagtca cgtccagga ggctgtcct 
gccacttgc tgttaaata ttacgggtc taaattcgc 
cgcgtggaa aaagcaact tttctcgtc aaatggtcg 
ctcaaaatc caggtttta caaagtccc gactgaaga 
ccaactagt tccagtgct aaggtccct tccccaaat 
ccccatcga tgattaccg tctggactc ggcaaacaa 
agagggaga gatggagga ttacgaggg gacgtatga 
tgactacct cgagctttt catccagtt cggttacat 
tttcctttt ctcgcctgt gtacccggt cgcggcctt 
ttgggcgct ttttaacaa cattttgga gatccgagc 
cgatgcgtt caaactttg caagatcta ccagcggcc 
gatgagtcg gcgggtgaa agacatagg cgcttggca 
gcgctgttt cgagataat cggcgccat gtcgataat 
gaccaactg cggcctttt gtgcctcag tccggagtt 
gaaaaggtc agccccgga agtgggccc ggtggagtg 
ggtgctgtt tttcgtgtt tctggagca cgttctgct 
cggcattcg atatctgct gcacattac catttcgga 
taagccgga atgggtgcg agtggccct agctaagaa 
gaattacga gtcgaaaca agccctcaa attcgagag 
gtcgcagaa aaacaaagg cctgctgag gagaaagtt 
caaaacgat acacgggac acacgcccg ttaataatt 
gaaagggag ttttacggg atcgatccg aatgtgcgt 
gtctgttcg gacaggacg aaaggataa gacacacct 
gtggagtcg gggtgccac ccgcgcgtg atgtatttt 
ttacaattt tttattgta caggggcaa taaatcgtt 
tcgcaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY