| General information | |||
| TC014620 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12498931..12508128 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014620 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05657 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014620 RA
MTSHTNRSN LSLPAQQMP PDAEFDATW AEEDDPHIH KLWAIHRTI HTLRGKMSK QQLDASDVM SDEFDENTS LPPTYIVIK IASDIKENT LTWLIDKIR GKRRDGGAE LIVMRQPSD PDDGWILHL SASKIKFLE TAEEMELMK EDKSGMMRE FTVGQLEDF LPEGMHVDD LLTLAEKQT IVRHELENI RALAEDEHI PGYPTYTLY EGQSIFQVC LKYKIIMKV YPLHDQEAL KKLGRKWYM SLLGKQPIE EIRLYFGEA IALYFTFLG FYTATLVVP VFVGLLQLM IYSETMSMF CVFNVVWVT LVLEIWKRK SNELAFKWG TISMTSLDE PRPNFRGSM GYDAITGKL QPQSPRYLT YLKMYCVSI PIVFLCLVA AFVMMLASF WLEEYFKQT RTSDDLIIL LPSIVYSIL VYIINCYYR KLATFLTEW ENHRTQSQH DRHRVTKLV LFEFVNNFM SLFYIAFVV RDMEMLRSQ LQTMLIIFQ LISHVQEAV LPLAVKYYG SKFAAWKKQ LFSSNGRSK SRFYKVPTE DQLVPVLRS LPQIPIDDY RLDSANKEG EMEDYEGTY DDYLELFIQ FGYIFLFSP VYPVAAFWA LFNNILEIR ADAFKLCKI YQRPMSRRV KDIGAWQRC FEIIGAMSI MTNCGLLCL SPELKRSAP EVGPVEWVL FFVFLEHVL LGIRYLLHI TISDKPEWV RVALAKKNY ESKQALKFE RSQKNKGLL RRKFKTIHG THAR
- >TC014620 RA
atgacatcc cacactaat cgctcgaat ctgtcactg ccggcgcaa caaatgcct ccagacgcg gaattcgat gcgacttgg gccgaagag gacgacccc cacatacac aaactgtgg gcgatacac agaacgatc cacacgctg agaggtaaa atgagtaaa caacaattg gacgcttca gacgtgatg tcggacgaa ttcgacgaa aacacctcc ctacctccc acttatatc gtcataaaa atcgcctcg gacatcaaa gaaaacacc ctaacgtgg ctgattgac aagattaga ggtaagcgt cgcgacggc ggcgccgaa ctcatcgtc atgcgacaa ccgagcgat cccgacgat ggttggatt ttgcacttg tcggcgtct aagatcaag ttcctcgag acggccgaa gagatggag ctgatgaaa gaggacaag agcggaatg atgcgtgag ttcaccgtg ggccaactg gaggatttc ctccctgaa ggaatgcac gtggacgat ttgttaacc cttgccgag aagcagacg atcgtccgc cacgaactg gaaaatatc cgagcattg gccgaggac gagcacatt cccggctac cccacgtac acactatac gagggccaa tcgatattt caagtgtgt ctcaagtac aaaataata atgaaggtt tatccgttg cacgatcaa gaggcgctg aagaagctg gggcggaag tggtacatg tcgttattg ggcaagcag ccgatcgaa gaaatccgg ttgtacttt ggtgaagct atagccttg tattttacg tttttgggc ttctatact gcaactttg gtggtgccc gtcttcgtg gggctcctc cagctgatg atctactcg gagacgatg tctatgttt tgtgtcttc aatgtggta tgggttaca cttgtctta gagatatgg aagagaaaa agcaacgag ctggcgttt aaatggggc accatcagc atgacgagt ctggacgag cccagaccc aacttccgg ggctcgatg ggctacgac gccatcacg gggaagctc cagccacag agccccaga tatctcacc tatctcaag atgtattgt gtgtcgatt cccatcgtg tttctgtgt cttgtggcg gctttcgtc atgatgttg gcctcgttt tggctcgag gagtatttc aaacagacg cggacttcc gacgactta atcatactt ctgccgagt atagtgtac tcgattcta gtctacata atcaactgt tattaccga aaattggcc acttttctg actgaatgg gaaaaccac cggactcag tcccaacac gaccgacat agagtgaca aagctggta ctttttgaa ttcgtcaac aacttcatg tcgctgttt tacattgcg ttcgtcgtg cgggacatg gaaatgttg cggagccaa ctccagaca atgctgatc atatttcag ttgatcagt cacgtccag gaggctgtc ctgccactt gctgttaaa tattacggg tctaaattc gccgcgtgg aaaaagcaa cttttctcg tcaaatggt cgctcaaaa tccaggttt tacaaagtc ccgactgaa gaccaacta gttccagtg ctaaggtcc cttccccaa atccccatc gatgattac cgtctggac tcggcaaac aaagaggga gagatggag gattacgag gggacgtat gatgactac ctcgagctt ttcatccag ttcggttac attttcctt ttctcgcct gtgtacccg gtcgcggcc ttttgggcg ctttttaac aacattttg gagatccga gccgatgcg ttcaaactt tgcaagatc taccagcgg ccgatgagt cggcgggtg aaagacata ggcgcttgg cagcgctgt ttcgagata atcggcgcc atgtcgata atgaccaac tgcggcctt ttgtgcctc agtccggag ttgaaaagg tcagccccg gaagtgggc ccggtggag tgggtgctg tttttcgtg tttctggag cacgttctg ctcggcatt cgatatctg ctgcacatt accatttcg gataagccg gaatgggtg cgagtggcc ctagctaag aagaattac gagtcgaaa caagccctc aaattcgag aggtcgcag aaaaacaaa ggcctgctg aggagaaag ttcaaaacg atacacggg acacacgcc cgttaa
- >TC014620 RA
ttaaattta cgatttgtt tacacaact gcgactttt tccacaata aaataaatt cctcgaact tctcaaccc ccacgtcca aaatcctgt tgaacaaat ctgaaacgg atcagttac cccacattt ggcccaaca acaattacc caaagcgct caaatagac aagcagcga acacatgat aacaaaata agagaagag gaaaacgag ccaatggcg cagactgaa ccactctga aagctccat ccccaacga aaaatttcg gtgacgttc cggtgacgc ctgaaaatc ccgctgcta agctacacc gtttgagtt ttgtcaaat ttttaaagc tcgcaccat ctcagcgcg agtctccgc agcaggaat ccgtccact gcaggaaac tagcagctc cggcacgtg gcgccctcc cggacccga tccaggtca gcactcttt ggttaggaa ttacaagtg cagctccca aatttcgaa ttttgcctc aagtatgtc gtaattcga atagttgca atcccgatc ctcgagcat gacatccca cactaatcg ctcgaatct gtcactgcc ggcgcaaca aatgcctcc agacgcgga attcgatgc gacttgggc cgaagagga cgaccccca catacacaa actgtgggc gatacacag aacgatcca cacgctgag aggtaaaat gagtaaaca acaattgga cgcttcaga cgtgatgtc ggacgaatt cgacgaaaa cacctccct acctcccac ttatatcgt cataaaaat cgcctcgga catcaaaga aaacaccct aacgtggct gattgacaa gattagagg taagcgtcg cgacggcgg cgccgaact catcgtcat gcgacaacc gagcgatcc cgacgatgg ttggatttt gcacttgtc ggcgtctaa gatcaagtt cctcgagac ggccgaaga gatggagct gatgaaaga ggacaagag cggaatgat gcgtgagtt caccgtggg ccaactgga ggatttcct ccctgaagg aatgcacgt ggacgattt gttaaccct tgccgagaa gcagacgat cgtccgcca cgaactgga aaatatccg agcattggc cgaggacga gcacattcc cggctaccc cacgtacac actatacga gggccaatc gatatttca agtgtgtct caagtacaa aataataat gaaggttta tccgttgca cgatcaaga ggcgctgaa gaagctggg gcggaagtg gtacatgtc gttattggg caagcagcc gatcgaaga aatccggtt gtactttgg tgaagctat agccttgta ttttacgtt tttgggctt ctatactgc aactttggt ggtgcccgt cttcgtggg gctcctcca gctgatgat ctactcgga gacgatgtc tatgttttg tgtcttcaa tgtggtatg ggttacact tgtcttaga gatatggaa gagaaaaag caacgagct ggcgtttaa atggggcac catcagcat gacgagtct ggacgagcc cagacccaa cttccgggg ctcgatggg ctacgacgc catcacggg gaagctcca gccacagag ccccagata tctcaccta tctcaagat gtattgtgt gtcgattcc catcgtgtt tctgtgtct tgtggcggc tttcgtcat gatgttggc ctcgttttg gctcgagga gtatttcaa acagacgcg gacttccga cgacttaat catacttct gccgagtat agtgtactc gattctagt ctacataat caactgtta ttaccgaaa attggccac ttttctgac tgaatggga aaaccaccg gactcagtc ccaacacga ccgacatag agtgacaaa gctggtact ttttgaatt cgtcaacaa cttcatgtc gctgtttta cattgcgtt cgtcgtgcg ggacatgga aatgttgcg gagccaact ccagacaat gctgatcat atttcagtt gatcagtca cgtccagga ggctgtcct gccacttgc tgttaaata ttacgggtc taaattcgc cgcgtggaa aaagcaact tttctcgtc aaatggtcg ctcaaaatc caggtttta caaagtccc gactgaaga ccaactagt tccagtgct aaggtccct tccccaaat ccccatcga tgattaccg tctggactc ggcaaacaa agagggaga gatggagga ttacgaggg gacgtatga tgactacct cgagctttt catccagtt cggttacat tttcctttt ctcgcctgt gtacccggt cgcggcctt ttgggcgct ttttaacaa cattttgga gatccgagc cgatgcgtt caaactttg caagatcta ccagcggcc gatgagtcg gcgggtgaa agacatagg cgcttggca gcgctgttt cgagataat cggcgccat gtcgataat gaccaactg cggcctttt gtgcctcag tccggagtt gaaaaggtc agccccgga agtgggccc ggtggagtg ggtgctgtt tttcgtgtt tctggagca cgttctgct cggcattcg atatctgct gcacattac catttcgga taagccgga atgggtgcg agtggccct agctaagaa gaattacga gtcgaaaca agccctcaa attcgagag gtcgcagaa aaacaaagg cctgctgag gagaaagtt caaaacgat acacgggac acacgcccg ttaataatt gaaagggag ttttacggg atcgatccg aatgtgcgt gtctgttcg gacaggacg aaaggataa gacacacct gtggagtcg gggtgccac ccgcgcgtg atgtatttt ttacaattt tttattgta caggggcaa taaatcgtt tcgcaacaa
