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Gene information
General information
TC014620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12498931..12508128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014620
    iBeetle screen iB_05657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014620 RA
      MTSHTNRSN LSLPAQQMP PDAEFDATW AEEDDPHIH 
      KLWAIHRTI HTLRGKMSK QQLDASDVM SDEFDENTS 
      LPPTYIVIK IASDIKENT LTWLIDKIR GKRRDGGAE 
      LIVMRQPSD PDDGWILHL SASKIKFLE TAEEMELMK 
      EDKSGMMRE FTVGQLEDF LPEGMHVDD LLTLAEKQT 
      IVRHELENI RALAEDEHI PGYPTYTLY EGQSIFQVC 
      LKYKIIMKV YPLHDQEAL KKLGRKWYM SLLGKQPIE 
      EIRLYFGEA IALYFTFLG FYTATLVVP VFVGLLQLM 
      IYSETMSMF CVFNVVWVT LVLEIWKRK SNELAFKWG 
      TISMTSLDE PRPNFRGSM GYDAITGKL QPQSPRYLT 
      YLKMYCVSI PIVFLCLVA AFVMMLASF WLEEYFKQT 
      RTSDDLIIL LPSIVYSIL VYIINCYYR KLATFLTEW 
      ENHRTQSQH DRHRVTKLV LFEFVNNFM SLFYIAFVV 
      RDMEMLRSQ LQTMLIIFQ LISHVQEAV LPLAVKYYG 
      SKFAAWKKQ LFSSNGRSK SRFYKVPTE DQLVPVLRS 
      LPQIPIDDY RLDSANKEG EMEDYEGTY DDYLELFIQ 
      FGYIFLFSP VYPVAAFWA LFNNILEIR ADAFKLCKI 
      YQRPMSRRV KDIGAWQRC FEIIGAMSI MTNCGLLCL 
      SPELKRSAP EVGPVEWVL FFVFLEHVL LGIRYLLHI 
      TISDKPEWV RVALAKKNY ESKQALKFE RSQKNKGLL 
      RRKFKTIHG THAR
    CDS sequence
    • >TC014620 RA
      atgacatcc cacactaat cgctcgaat ctgtcactg 
      ccggcgcaa caaatgcct ccagacgcg gaattcgat 
      gcgacttgg gccgaagag gacgacccc cacatacac 
      aaactgtgg gcgatacac agaacgatc cacacgctg 
      agaggtaaa atgagtaaa caacaattg gacgcttca 
      gacgtgatg tcggacgaa ttcgacgaa aacacctcc 
      ctacctccc acttatatc gtcataaaa atcgcctcg 
      gacatcaaa gaaaacacc ctaacgtgg ctgattgac 
      aagattaga ggtaagcgt cgcgacggc ggcgccgaa 
      ctcatcgtc atgcgacaa ccgagcgat cccgacgat 
      ggttggatt ttgcacttg tcggcgtct aagatcaag 
      ttcctcgag acggccgaa gagatggag ctgatgaaa 
      gaggacaag agcggaatg atgcgtgag ttcaccgtg 
      ggccaactg gaggatttc ctccctgaa ggaatgcac 
      gtggacgat ttgttaacc cttgccgag aagcagacg 
      atcgtccgc cacgaactg gaaaatatc cgagcattg 
      gccgaggac gagcacatt cccggctac cccacgtac 
      acactatac gagggccaa tcgatattt caagtgtgt 
      ctcaagtac aaaataata atgaaggtt tatccgttg 
      cacgatcaa gaggcgctg aagaagctg gggcggaag 
      tggtacatg tcgttattg ggcaagcag ccgatcgaa 
      gaaatccgg ttgtacttt ggtgaagct atagccttg 
      tattttacg tttttgggc ttctatact gcaactttg 
      gtggtgccc gtcttcgtg gggctcctc cagctgatg 
      atctactcg gagacgatg tctatgttt tgtgtcttc 
      aatgtggta tgggttaca cttgtctta gagatatgg 
      aagagaaaa agcaacgag ctggcgttt aaatggggc 
      accatcagc atgacgagt ctggacgag cccagaccc 
      aacttccgg ggctcgatg ggctacgac gccatcacg 
      gggaagctc cagccacag agccccaga tatctcacc 
      tatctcaag atgtattgt gtgtcgatt cccatcgtg 
      tttctgtgt cttgtggcg gctttcgtc atgatgttg 
      gcctcgttt tggctcgag gagtatttc aaacagacg 
      cggacttcc gacgactta atcatactt ctgccgagt 
      atagtgtac tcgattcta gtctacata atcaactgt 
      tattaccga aaattggcc acttttctg actgaatgg 
      gaaaaccac cggactcag tcccaacac gaccgacat 
      agagtgaca aagctggta ctttttgaa ttcgtcaac 
      aacttcatg tcgctgttt tacattgcg ttcgtcgtg 
      cgggacatg gaaatgttg cggagccaa ctccagaca 
      atgctgatc atatttcag ttgatcagt cacgtccag 
      gaggctgtc ctgccactt gctgttaaa tattacggg 
      tctaaattc gccgcgtgg aaaaagcaa cttttctcg 
      tcaaatggt cgctcaaaa tccaggttt tacaaagtc 
      ccgactgaa gaccaacta gttccagtg ctaaggtcc 
      cttccccaa atccccatc gatgattac cgtctggac 
      tcggcaaac aaagaggga gagatggag gattacgag 
      gggacgtat gatgactac ctcgagctt ttcatccag 
      ttcggttac attttcctt ttctcgcct gtgtacccg 
      gtcgcggcc ttttgggcg ctttttaac aacattttg 
      gagatccga gccgatgcg ttcaaactt tgcaagatc 
      taccagcgg ccgatgagt cggcgggtg aaagacata 
      ggcgcttgg cagcgctgt ttcgagata atcggcgcc 
      atgtcgata atgaccaac tgcggcctt ttgtgcctc 
      agtccggag ttgaaaagg tcagccccg gaagtgggc 
      ccggtggag tgggtgctg tttttcgtg tttctggag 
      cacgttctg ctcggcatt cgatatctg ctgcacatt 
      accatttcg gataagccg gaatgggtg cgagtggcc 
      ctagctaag aagaattac gagtcgaaa caagccctc 
      aaattcgag aggtcgcag aaaaacaaa ggcctgctg 
      aggagaaag ttcaaaacg atacacggg acacacgcc 
      cgttaa
    mRNA sequence
    • >TC014620 RA
      ttaaattta cgatttgtt tacacaact gcgactttt 
      tccacaata aaataaatt cctcgaact tctcaaccc 
      ccacgtcca aaatcctgt tgaacaaat ctgaaacgg 
      atcagttac cccacattt ggcccaaca acaattacc 
      caaagcgct caaatagac aagcagcga acacatgat 
      aacaaaata agagaagag gaaaacgag ccaatggcg 
      cagactgaa ccactctga aagctccat ccccaacga 
      aaaatttcg gtgacgttc cggtgacgc ctgaaaatc 
      ccgctgcta agctacacc gtttgagtt ttgtcaaat 
      ttttaaagc tcgcaccat ctcagcgcg agtctccgc 
      agcaggaat ccgtccact gcaggaaac tagcagctc 
      cggcacgtg gcgccctcc cggacccga tccaggtca 
      gcactcttt ggttaggaa ttacaagtg cagctccca 
      aatttcgaa ttttgcctc aagtatgtc gtaattcga 
      atagttgca atcccgatc ctcgagcat gacatccca 
      cactaatcg ctcgaatct gtcactgcc ggcgcaaca 
      aatgcctcc agacgcgga attcgatgc gacttgggc 
      cgaagagga cgaccccca catacacaa actgtgggc 
      gatacacag aacgatcca cacgctgag aggtaaaat 
      gagtaaaca acaattgga cgcttcaga cgtgatgtc 
      ggacgaatt cgacgaaaa cacctccct acctcccac 
      ttatatcgt cataaaaat cgcctcgga catcaaaga 
      aaacaccct aacgtggct gattgacaa gattagagg 
      taagcgtcg cgacggcgg cgccgaact catcgtcat 
      gcgacaacc gagcgatcc cgacgatgg ttggatttt 
      gcacttgtc ggcgtctaa gatcaagtt cctcgagac 
      ggccgaaga gatggagct gatgaaaga ggacaagag 
      cggaatgat gcgtgagtt caccgtggg ccaactgga 
      ggatttcct ccctgaagg aatgcacgt ggacgattt 
      gttaaccct tgccgagaa gcagacgat cgtccgcca 
      cgaactgga aaatatccg agcattggc cgaggacga 
      gcacattcc cggctaccc cacgtacac actatacga 
      gggccaatc gatatttca agtgtgtct caagtacaa 
      aataataat gaaggttta tccgttgca cgatcaaga 
      ggcgctgaa gaagctggg gcggaagtg gtacatgtc 
      gttattggg caagcagcc gatcgaaga aatccggtt 
      gtactttgg tgaagctat agccttgta ttttacgtt 
      tttgggctt ctatactgc aactttggt ggtgcccgt 
      cttcgtggg gctcctcca gctgatgat ctactcgga 
      gacgatgtc tatgttttg tgtcttcaa tgtggtatg 
      ggttacact tgtcttaga gatatggaa gagaaaaag 
      caacgagct ggcgtttaa atggggcac catcagcat 
      gacgagtct ggacgagcc cagacccaa cttccgggg 
      ctcgatggg ctacgacgc catcacggg gaagctcca 
      gccacagag ccccagata tctcaccta tctcaagat 
      gtattgtgt gtcgattcc catcgtgtt tctgtgtct 
      tgtggcggc tttcgtcat gatgttggc ctcgttttg 
      gctcgagga gtatttcaa acagacgcg gacttccga 
      cgacttaat catacttct gccgagtat agtgtactc 
      gattctagt ctacataat caactgtta ttaccgaaa 
      attggccac ttttctgac tgaatggga aaaccaccg 
      gactcagtc ccaacacga ccgacatag agtgacaaa 
      gctggtact ttttgaatt cgtcaacaa cttcatgtc 
      gctgtttta cattgcgtt cgtcgtgcg ggacatgga 
      aatgttgcg gagccaact ccagacaat gctgatcat 
      atttcagtt gatcagtca cgtccagga ggctgtcct 
      gccacttgc tgttaaata ttacgggtc taaattcgc 
      cgcgtggaa aaagcaact tttctcgtc aaatggtcg 
      ctcaaaatc caggtttta caaagtccc gactgaaga 
      ccaactagt tccagtgct aaggtccct tccccaaat 
      ccccatcga tgattaccg tctggactc ggcaaacaa 
      agagggaga gatggagga ttacgaggg gacgtatga 
      tgactacct cgagctttt catccagtt cggttacat 
      tttcctttt ctcgcctgt gtacccggt cgcggcctt 
      ttgggcgct ttttaacaa cattttgga gatccgagc 
      cgatgcgtt caaactttg caagatcta ccagcggcc 
      gatgagtcg gcgggtgaa agacatagg cgcttggca 
      gcgctgttt cgagataat cggcgccat gtcgataat 
      gaccaactg cggcctttt gtgcctcag tccggagtt 
      gaaaaggtc agccccgga agtgggccc ggtggagtg 
      ggtgctgtt tttcgtgtt tctggagca cgttctgct 
      cggcattcg atatctgct gcacattac catttcgga 
      taagccgga atgggtgcg agtggccct agctaagaa 
      gaattacga gtcgaaaca agccctcaa attcgagag 
      gtcgcagaa aaacaaagg cctgctgag gagaaagtt 
      caaaacgat acacgggac acacgcccg ttaataatt 
      gaaagggag ttttacggg atcgatccg aatgtgcgt 
      gtctgttcg gacaggacg aaaggataa gacacacct 
      gtggagtcg gggtgccac ccgcgcgtg atgtatttt 
      ttacaattt tttattgta caggggcaa taaatcgtt 
      tcgcaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY