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Gene information
General information
TC014616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12374861..12405336
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014616
    iBeetle screen iB_05655

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014616 RA
      MVLGLLILA CLWGPLSAL CPSQCSCYL DPRGRNAVL 
      CKDGGIVGP LDLFNMSAD TEVLKITAP EDNMNQLTM 
      SPIFSNYKN LEEIHITRS NIPQLGMHF FLKLKKLDV 
      LDLSQNNIT QPLDHNFRG LMRLKELYL DDNRISSLP 
      SGTFRFLEE LKVLSIQRN RIAELTPRI FLEIGKLKV 
      LKLSGNHLQ ELNPVVFED VRELKSLEC RSCGLAQID 
      KQVYELLPH LTHLDLGDN LLTSITPEE FSPLTNLRH 
      LKLDGNRIT AIKSSVFPN QHELKRLTL ARNRITKIS 
      RNAFANLHN LTELDLSHN KFENPENGL LEPVAATLE 
      VLSLNGNHL SVPTLKSLL NDLRIKELH LADCGLFEL 
      GKLVPDTVQ VLSLGNNYI SSLAPEALP ESLAELDIT 
      GNKFVGLDN DLLARLDHL KRLELDGNP WACDLCHIV 
      PLLERANRS SAVRNLQCA QPYSLKGMK LGALHFHEL 
      TWCTVPSTA SDANFFLLG DDGRLGLIA AGTSVALLF 
      FTVVGVLGV LLYSRRHAA KYYTHEDKL AMDGDSIFE 
      NHSPLFGDE RELSFKFPL DGERKVSVA TIDEIKKEH 
      AISNGT
    CDS sequence
    • >TC014616 RA
      atggttttg ggactgttg atcctcgcg tgcctctgg 
      gggcccttg tcggcgctg tgcccgtcg cagtgtagc 
      tgctacctg gaccccagg gggcgcaat gccgtcctg 
      tgcaaggac ggcggtatc gtcggccct ctcgacctc 
      ttcaacatg agcgccgac acggaagtg ctgaaaata 
      acggcgccg gaagacaac atgaatcag ctcaccatg 
      agccccatc ttctccaac tacaagaac ttggaggag 
      attcacatc acgcgctcc aacattccc caattggga 
      atgcacttt tttctcaaa ttgaagaag ctggacgtg 
      ctggacctg tcccagaac aacatcacg cagccgctc 
      gatcacaac ttcagggga ttgatgagg ctgaaggag 
      ctgtacttg gacgataac aggatcagc tcgctgccg 
      agcggcacg ttccgcttc ttggaggag ctgaaggtc 
      ttgtccatt cagaggaac aggattgcg gagttgacg 
      ccgaggatt tttctagag attggcaag ttgaaggtg 
      ctgaagctg agcgggaat cacctgcag gagctgaat 
      ccggttgtc ttcgaggac gtgagggag ttgaaatct 
      ctggaatgc cgttcttgc ggtttggcg caaatcgat 
      aagcaagtg tacgagttg ttgccgcat ctgacgcac 
      ttagatctg ggtgataac ttgctgact tcaatcaca 
      cccgaggag ttttcgccg ttgactaat ctgcggcat 
      ttgaaattg gacgggaac cgaatcact gccataaaa 
      agctcagta tttcccaat caacacgag ttgaaaaga 
      ctgactctc gcacgcaac cgcatcacg aaaatatct 
      cgcaacgct ttcgccaat ttacataat ctcaccgaa 
      ttggacttg agtcacaat aagttcgag aatcccgaa 
      aacggcctg ttagaacct gttgctgcc actttggag 
      gttctttca ctcaatgga aatcattta agtgttccc 
      accttgaaa agcttgcta aatgacctc cgaataaaa 
      gaactacat ttagccgat tgcggcctc ttcgagctg 
      gggaaactc gttccagac accgttcaa gtactaagt 
      ctcggaaac aattacatt tcctcgtta gcaccggaa 
      gcgctcccg gaaagtctc gccgaactc gacataacc 
      ggaaacaaa ttcgtcggt ttggataac gacctgttg 
      gccagactt gaccacttg aagcgcctc gagctggac 
      ggcaaccct tgggcctgc gacctgtgc cacatcgtg 
      ccgttgctg gaaagggcc aaccggagc tccgccgtc 
      cggaacctc cagtgcgcc cagccctac tccctcaaa 
      ggcatgaag ctcggagcc ttgcacttc cacgagctg 
      acctggtgc accgtcccg tcgacggcc agcgacgcc 
      aacttcttc ctcctcggc gacgacggc cggctgggg 
      ctgatcgcc gctggaacg tcggtggcg ctcctcttc 
      ttcacagtg gtgggtgtg ctgggggtg ctgctttac 
      tcgcgccgg cacgccgcc aagtactac acccacgag 
      gacaagctg gccatggac ggagactcc atcttcgag 
      aatcacagt cctctcttt ggcgacgag cgcgagctc 
      agcttcaaa ttccccctg gacggtgag cgaaaagtg 
      tccgtggcg accatcgac gagataaaa aaagaacat 
      gcaatcagt aacgggacg tga
    mRNA sequence
    • >TC014616 RA
      tgcttacta cgatgcaaa gcggtgtag tgggtgttt 
      tttttttaa attagaagt gcacgtggt ttaatttcg 
      tggcctagt tgacctcca ctagtgtac caagtgttg 
      ctgaatgat ttttgctta attaccacc cgtttccgg 
      tgtagcatg gttttggga ctgttgatc ctcgcgtgc 
      ctctggggg cccttgtcg gcgctgtgc ccgtcgcag 
      tgtagctgc tacctggac cccaggggg cgcaatgcc 
      gtcctgtgc aaggacggc ggtatcgtc ggccctctc 
      gacctcttc aacatgagc gccgacacg gaagtgctg 
      aaaataacg gcgccggaa gacaacatg aatcagctc 
      accatgagc cccatcttc tccaactac aagaacttg 
      gaggagatt cacatcacg cgctccaac attccccaa 
      ttgggaatg cactttttt ctcaaattg aagaagctg 
      gacgtgctg gacctgtcc cagaacaac atcacgcag 
      ccgctcgat cacaacttc aggggattg atgaggctg 
      aaggagctg tacttggac gataacagg atcagctcg 
      ctgccgagc ggcacgttc cgcttcttg gaggagctg 
      aaggtcttg tccattcag aggaacagg attgcggag 
      ttgacgccg aggattttt ctagagatt ggcaagttg 
      aaggtgctg aagctgagc gggaatcac ctgcaggag 
      ctgaatccg gttgtcttc gaggacgtg agggagttg 
      aaatctctg gaatgccgt tcttgcggt ttggcgcaa 
      atcgataag caagtgtac gagttgttg ccgcatctg 
      acgcactta gatctgggt gataacttg ctgacttca 
      atcacaccc gaggagttt tcgccgttg actaatctg 
      cggcatttg aaattggac gggaaccga atcactgcc 
      ataaaaagc tcagtattt cccaatcaa cacgagttg 
      aaaagactg actctcgca cgcaaccgc atcacgaaa 
      atatctcgc aacgctttc gccaattta cataatctc 
      accgaattg gacttgagt cacaataag ttcgagaat 
      cccgaaaac ggcctgtta gaacctgtt gctgccact 
      ttggaggtt ctttcactc aatggaaat catttaagt 
      gttcccacc ttgaaaagc ttgctaaat gacctccga 
      ataaaagaa ctacattta gccgattgc ggcctcttc 
      gagctgggg aaactcgtt ccagacacc gttcaagta 
      ctaagtctc ggaaacaat tacatttcc tcgttagca 
      ccggaagcg ctcccggaa agtctcgcc gaactcgac 
      ataaccgga aacaaattc gtcggtttg gataacgac 
      ctgttggcc agacttgac cacttgaag cgcctcgag 
      ctggacggc aacccttgg gcctgcgac ctgtgccac 
      atcgtgccg ttgctggaa agggccaac cggagctcc 
      gccgtccgg aacctccag tgcgcccag ccctactcc 
      ctcaaaggc atgaagctc ggagccttg cacttccac 
      gagctgacc tggtgcacc gtcccgtcg acggccagc 
      gacgccaac ttcttcctc ctcggcgac gacggccgg 
      ctggggctg atcgccgct ggaacgtcg gtggcgctc 
      ctcttcttc acagtggtg ggtgtgctg ggggtgctg 
      ctttactcg cgccggcac gccgccaag tactacacc 
      cacgaggac aagctggcc atggacgga gactccatc 
      ttcgagaat cacagtcct ctctttggc gacgagcgc 
      gagctcagc ttcaaattc cccctggac ggtgagcga 
      aaagtgtcc gtggcgacc atcgacgag ataaaaaaa 
      gaacatgca atcagtaac gggacgtga aagactgat 
      aattccgtt ccgtagtgt ttgtgacga gagtttcct 
      cttttgtaa atatcattt cgacgagtg gtgcccagt 
      gaatgaacg gctgtcttc cttttttcg gaaacgttt 
      gaaacgagc tgtgaatcg tgctaaatt aatggagat 
      ggaatattt ttgagaaac aacgagtct gtcaatttt 
      atacggcct attttgtgt aaaacactg cttttttga 
      catcgtcaa aataatcat tcctggctg cattttgtg 
      taaagtcgc gtcagtcct gtgacttgt tcctgtcac 
      tttcctcga tgcgtcgct aataagtac ttaatttaa 
      gttaacgtg tgatttgtc ttaacaatg atattttga 
      gtaagaagc ttttacaat ttatataat tttagatgg 
      tatatattt atgaaccgc actgtacaa aatgtgaga 
      ttgttaatt aaattaaaa ttaatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY