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Gene information
General information
TC014604 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12292490..12322419
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014604
    iBeetle screen iB_05654

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014604 RA
      MLGLSVEQC ACLVFLVVL AACSGFTNA MPRSRSVSE 
      VPPRLRHRR SCPRIDAAW KAYRAGTVA LATAQSLST 
      DCRNASRDE CQVLFTVLK WYKREPPAS AARDSGELI 
      PLRLHKNNT SKYCEYDNF ATRAKPLVR TKIENSQQY 
      ILFLNTSGP HKFSAAFVP ERYTSKMER AIVKVTNDN 
      FTPKPVRIN ESACHARHR KYTCRVEGF PLPTVQWKH 
      NNTILHNRD RVRIIQKKR KSILVVDKR SDTGQYTCV 
      AIAATGEEV SYVVRRNSS PSKSKTHNS KHKMHSTSR 
      PKTHRIFRP NTHTTSGPL QTPGTYTSD IDLATPAPD 
      REPCKEDMR ENFCHNGGT CFATRITGG KVPNSYDCV 
      CPPGYTGHR CEGKMSDQS IL
    • >TC014604 RB
      MLGLSVEQC ACLVFLVVL AACSGFTNA MPRSRSVSE 
      VPPRLRHRR SCPRIDAAW KAYRAGTVA LATAQSLST 
      DCRNASRDE CQVLFTVLK WYKREPPAS AARDSGELI 
      PLRLHKNNT SKYCEYDNF ATRAKPLVR TKIENSQQY 
      ILFLNTSGP HKFSAAFVP ERYTSKMER AIVKVTNDN 
      FTPKPVRIN ESACHARHR KYTCRVEGF PLPTVQWKH 
      NNTILHNRD RVRIIQKKR KSILVVDKR SDTGQYTCV 
      AIAATGEEV SYVVRRNSS PSKSKTHNS KHKMHSTSR 
      PKTHRIFRP NTHTTSGPL QTPGEPCKE DMRENFCHN 
      GGTCFATRI TGGKVPNSY DCVCPPGYT GHRCEGKMS 
      DQSIL
    • >TC014604 RC
      MLGLSVEQC ACLVFLVVL AACSGFTNA MPRSRSVSE 
      VPPRLRHRR SCPRIDAAW KAYRAGTVA LATAQSLST 
      DCRNASRDE CQVLFTVLK WYKREPPAS AARDSGELI 
      PLRLHKNNT SKYCEYDNF ATRAKPLVR TKIENSQQY 
      ILFLNTSGP HKFSAAFVP ERYTSKMER AIVKVTNDN 
      FTPKPVRIN ESACHARHR KYTCRVEGF PLPTVQWKH 
      NNTILHNRD RVRIIQKKR KSILVVDKR SDTGQYTCV 
      AIAATGEEV SYVVRRNSS PSKSKTHNS KHKMHSTSR 
      PKTHRIFRP NTHTTSGPL QTPGEPCKE DMRENFCHN 
      GGTCFATRI TGGKVPNSY DCVCPPGYT GHRCEGKMS 
      DQSSKKATD SYCEGAEKS DKAG
    CDS sequence
    • >TC014604 RA
      atgttagga ttatcggtg gagcagtgt gcatgtctc 
      gtcttccta gtggttctc gcagcttgt tccggtttc 
      acaaacgct atgcccagg agtcgctca gtgagtgaa 
      gtgccgccg cggctgcgc caccgccgc tcctgcccc 
      aggatcgac gccgcgtgg aaggcgtac agggccggc 
      accgtcgcg ctcgccacg gcccagagc ctgagcacc 
      gactgcagg aacgccagc agggacgaa tgtcaggtg 
      ctgttcacg gtgctcaag tggtacaaa cgcgaaccc 
      ccagcctcg gccgccagg gactccggg gagctcata 
      ccgctgcgg ctgcacaag aacaacacc agcaagtac 
      tgcgagtac gacaacttc gccaccagg gcgaaaccc 
      ctagtcagg accaaaatc gaaaactca caacagtac 
      atactcttc ctcaacacc tccgggccg cacaagttc 
      tccgccgct tttgtaccg gaacgatac acaagcaaa 
      atggaaaga gccatcgtc aaagtcact aatgataac 
      tttactccg aagcctgtc aggatcaac gagagcgcc 
      tgccacgcc aggcaccga aaatacacg tgtagagtc 
      gagggattc cccttgccc acagtccag tggaagcac 
      aacaacacg atcctccac aacagggac cgcgtccgc 
      atcattcag aaaaagcga aaaagtatt ctggtggtc 
      gacaaacgc tcggatacg ggacagtac acctgtgtg 
      gcgattgca gcgactgga gaggaggtt tcctatgtc 
      gtccgccgc aactcgtcg ccgtcgaag tccaagaca 
      cacaactcc aagcacaaa atgcactcg acctccagg 
      cccaagacg cacaggatc ttcaggccc aacacacac 
      acgacctcc gggccttta cagacacct ggtacctat 
      acaagcgat atagattta gcgacacca gcgcctgat 
      agagaaccc tgcaaggaa gacatgcga gagaacttt 
      tgtcataat gggggcact tgctttgcc acgagaatc 
      acaggcggc aaagtaccg aattcctat gattgtgtg 
      tgtcctccc gggtacacg ggtcataga tgtgagggg 
      aaaatgtcc gatcaatcc atattgtga
    • >TC014604 RB
      atgttagga ttatcggtg gagcagtgt gcatgtctc 
      gtcttccta gtggttctc gcagcttgt tccggtttc 
      acaaacgct atgcccagg agtcgctca gtgagtgaa 
      gtgccgccg cggctgcgc caccgccgc tcctgcccc 
      aggatcgac gccgcgtgg aaggcgtac agggccggc 
      accgtcgcg ctcgccacg gcccagagc ctgagcacc 
      gactgcagg aacgccagc agggacgaa tgtcaggtg 
      ctgttcacg gtgctcaag tggtacaaa cgcgaaccc 
      ccagcctcg gccgccagg gactccggg gagctcata 
      ccgctgcgg ctgcacaag aacaacacc agcaagtac 
      tgcgagtac gacaacttc gccaccagg gcgaaaccc 
      ctagtcagg accaaaatc gaaaactca caacagtac 
      atactcttc ctcaacacc tccgggccg cacaagttc 
      tccgccgct tttgtaccg gaacgatac acaagcaaa 
      atggaaaga gccatcgtc aaagtcact aatgataac 
      tttactccg aagcctgtc aggatcaac gagagcgcc 
      tgccacgcc aggcaccga aaatacacg tgtagagtc 
      gagggattc cccttgccc acagtccag tggaagcac 
      aacaacacg atcctccac aacagggac cgcgtccgc 
      atcattcag aaaaagcga aaaagtatt ctggtggtc 
      gacaaacgc tcggatacg ggacagtac acctgtgtg 
      gcgattgca gcgactgga gaggaggtt tcctatgtc 
      gtccgccgc aactcgtcg ccgtcgaag tccaagaca 
      cacaactcc aagcacaaa atgcactcg acctccagg 
      cccaagacg cacaggatc ttcaggccc aacacacac 
      acgacctcc gggccttta cagacacct ggagaaccc 
      tgcaaggaa gacatgcga gagaacttt tgtcataat 
      gggggcact tgctttgcc acgagaatc acaggcggc 
      aaagtaccg aattcctat gattgtgtg tgtcctccc 
      gggtacacg ggtcataga tgtgagggg aaaatgtcc 
      gatcaatcc atattgtga
    • >TC014604 RC
      atgttagga ttatcggtg gagcagtgt gcatgtctc 
      gtcttccta gtggttctc gcagcttgt tccggtttc 
      acaaacgct atgcccagg agtcgctca gtgagtgaa 
      gtgccgccg cggctgcgc caccgccgc tcctgcccc 
      aggatcgac gccgcgtgg aaggcgtac agggccggc 
      accgtcgcg ctcgccacg gcccagagc ctgagcacc 
      gactgcagg aacgccagc agggacgaa tgtcaggtg 
      ctgttcacg gtgctcaag tggtacaaa cgcgaaccc 
      ccagcctcg gccgccagg gactccggg gagctcata 
      ccgctgcgg ctgcacaag aacaacacc agcaagtac 
      tgcgagtac gacaacttc gccaccagg gcgaaaccc 
      ctagtcagg accaaaatc gaaaactca caacagtac 
      atactcttc ctcaacacc tccgggccg cacaagttc 
      tccgccgct tttgtaccg gaacgatac acaagcaaa 
      atggaaaga gccatcgtc aaagtcact aatgataac 
      tttactccg aagcctgtc aggatcaac gagagcgcc 
      tgccacgcc aggcaccga aaatacacg tgtagagtc 
      gagggattc cccttgccc acagtccag tggaagcac 
      aacaacacg atcctccac aacagggac cgcgtccgc 
      atcattcag aaaaagcga aaaagtatt ctggtggtc 
      gacaaacgc tcggatacg ggacagtac acctgtgtg 
      gcgattgca gcgactgga gaggaggtt tcctatgtc 
      gtccgccgc aactcgtcg ccgtcgaag tccaagaca 
      cacaactcc aagcacaaa atgcactcg acctccagg 
      cccaagacg cacaggatc ttcaggccc aacacacac 
      acgacctcc gggccttta cagacacct ggagaaccc 
      tgcaaggaa gacatgcga gagaacttt tgtcataat 
      gggggcact tgctttgcc acgagaatc acaggcggc 
      aaagtaccg aattcctat gattgtgtg tgtcctccc 
      gggtacacg ggtcataga tgtgagggg aaaatgtcc 
      gatcaatcc agcaaaaaa gcgaccgat tcgtattgt 
      gagggagcg gaaaagagc gacaaggct ggataa
    mRNA sequence
    • >TC014604 RA
      ctcttattc tcgttgcgg cgtctgtcg gcggccgct 
      ttggagggg gacttcagt cagtgtagg ttgtacttc 
      tcagccgtc aacatcgcc ctcgacggc gctcgtagt 
      cgttcctcg tgctggtgc tcgtcgtgg tcgtcgtcg 
      cggtggtgt ttggtgatg tggaggtgc gtcagtgcg 
      tgaccggga tgacgttgg gcggcgccg tcgccggcc 
      ccaggtgca cacatgcag tgtctccga tcggggggc 
      tccgggccg tgagatcgc cgtgagtac gcgacctgc 
      agacatgtt aggattatc ggtggagca gtgtgcatg 
      tctcgtctt cctagtggt tctcgcagc ttgttccgg 
      tttcacaaa cgctatgcc caggagtcg ctcagtgag 
      tgaagtgcc gccgcggct gcgccaccg ccgctcctg 
      ccccaggat cgacgccgc gtggaaggc gtacagggc 
      cggcaccgt cgcgctcgc cacggccca gagcctgag 
      caccgactg caggaacgc cagcaggga cgaatgtca 
      ggtgctgtt cacggtgct caagtggta caaacgcga 
      acccccagc ctcggccgc cagggactc cggggagct 
      cataccgct gcggctgca caagaacaa caccagcaa 
      gtactgcga gtacgacaa cttcgccac cagggcgaa 
      acccctagt caggaccaa aatcgaaaa ctcacaaca 
      gtacatact cttcctcaa cacctccgg gccgcacaa 
      gttctccgc cgcttttgt accggaacg atacacaag 
      caaaatgga aagagccat cgtcaaagt cactaatga 
      taactttac tccgaagcc tgtcaggat caacgagag 
      cgcctgcca cgccaggca ccgaaaata cacgtgtag 
      agtcgaggg attcccctt gcccacagt ccagtggaa 
      gcacaacaa cacgatcct ccacaacag ggaccgcgt 
      ccgcatcat tcagaaaaa gcgaaaaag tattctggt 
      ggtcgacaa acgctcgga tacgggaca gtacacctg 
      tgtggcgat tgcagcgac tggagagga ggtttccta 
      tgtcgtccg ccgcaactc gtcgccgtc gaagtccaa 
      gacacacaa ctccaagca caaaatgca ctcgacctc 
      caggcccaa gacgcacag gatcttcag gcccaacac 
      acacacgac ctccgggcc tttacagac acctggtac 
      ctatacaag cgatataga tttagcgac accagcgcc 
      tgatagaga accctgcaa ggaagacat gcgagagaa 
      cttttgtca taatggggg cacttgctt tgccacgag 
      aatcacagg cggcaaagt accgaattc ctatgattg 
      tgtgtgtcc tcccgggta cacgggtca tagatgtga 
      ggggaaaat gtccgatca atccatatt gtgagggag 
      cggaaaaga gcgacaagg ctggataac cctatacca 
      aagaaaatt gcattacag aggatgtag atactcgtt 
      taatttatt tgatttgga gaaaaacgc acaatgtag 
      tagtcgatg tgtaataat ttttttatc atgtt
    • >TC014604 RB
      ctcttattc tcgttgcgg cgtctgtcg gcggccgct 
      ttggagggg gacttcagt cagtgtagg ttgtacttc 
      tcagccgtc aacatcgcc ctcgacggc gctcgtagt 
      cgttcctcg tgctggtgc tcgtcgtgg tcgtcgtcg 
      cggtggtgt ttggtgatg tggaggtgc gtcagtgcg 
      tgaccggga tgacgttgg gcggcgccg tcgccggcc 
      ccaggtgca cacatgcag tgtctccga tcggggggc 
      tccgggccg tgagatcgc cgtgagtac gcgacctgc 
      agacatgtt aggattatc ggtggagca gtgtgcatg 
      tctcgtctt cctagtggt tctcgcagc ttgttccgg 
      tttcacaaa cgctatgcc caggagtcg ctcagtgag 
      tgaagtgcc gccgcggct gcgccaccg ccgctcctg 
      ccccaggat cgacgccgc gtggaaggc gtacagggc 
      cggcaccgt cgcgctcgc cacggccca gagcctgag 
      caccgactg caggaacgc cagcaggga cgaatgtca 
      ggtgctgtt cacggtgct caagtggta caaacgcga 
      acccccagc ctcggccgc cagggactc cggggagct 
      cataccgct gcggctgca caagaacaa caccagcaa 
      gtactgcga gtacgacaa cttcgccac cagggcgaa 
      acccctagt caggaccaa aatcgaaaa ctcacaaca 
      gtacatact cttcctcaa cacctccgg gccgcacaa 
      gttctccgc cgcttttgt accggaacg atacacaag 
      caaaatgga aagagccat cgtcaaagt cactaatga 
      taactttac tccgaagcc tgtcaggat caacgagag 
      cgcctgcca cgccaggca ccgaaaata cacgtgtag 
      agtcgaggg attcccctt gcccacagt ccagtggaa 
      gcacaacaa cacgatcct ccacaacag ggaccgcgt 
      ccgcatcat tcagaaaaa gcgaaaaag tattctggt 
      ggtcgacaa acgctcgga tacgggaca gtacacctg 
      tgtggcgat tgcagcgac tggagagga ggtttccta 
      tgtcgtccg ccgcaactc gtcgccgtc gaagtccaa 
      gacacacaa ctccaagca caaaatgca ctcgacctc 
      caggcccaa gacgcacag gatcttcag gcccaacac 
      acacacgac ctccgggcc tttacagac acctggaga 
      accctgcaa ggaagacat gcgagagaa cttttgtca 
      taatggggg cacttgctt tgccacgag aatcacagg 
      cggcaaagt accgaattc ctatgattg tgtgtgtcc 
      tcccgggta cacgggtca tagatgtga ggggaaaat 
      gtccgatca atccatatt gtgagggag cggaaaaga 
      gcgacaagg ctggataac cctatacca aagaaaatt 
      gcattacag aggatgtag atactcgtt taatttatt 
      tgatttgga gaaaaacgc acaatgtag tagtcgatg 
      tgtaataat ttttttatc atgtt
    • >TC014604 RC
      ctcttattc tcgttgcgg cgtctgtcg gcggccgct 
      ttggagggg gacttcagt cagtgtagg ttgtacttc 
      tcagccgtc aacatcgcc ctcgacggc gctcgtagt 
      cgttcctcg tgctggtgc tcgtcgtgg tcgtcgtcg 
      cggtggtgt ttggtgatg tggaggtgc gtcagtgcg 
      tgaccggga tgacgttgg gcggcgccg tcgccggcc 
      ccaggtgca cacatgcag tgtctccga tcggggggc 
      tccgggccg tgagatcgc cgtgagtac gcgacctgc 
      agacatgtt aggattatc ggtggagca gtgtgcatg 
      tctcgtctt cctagtggt tctcgcagc ttgttccgg 
      tttcacaaa cgctatgcc caggagtcg ctcagtgag 
      tgaagtgcc gccgcggct gcgccaccg ccgctcctg 
      ccccaggat cgacgccgc gtggaaggc gtacagggc 
      cggcaccgt cgcgctcgc cacggccca gagcctgag 
      caccgactg caggaacgc cagcaggga cgaatgtca 
      ggtgctgtt cacggtgct caagtggta caaacgcga 
      acccccagc ctcggccgc cagggactc cggggagct 
      cataccgct gcggctgca caagaacaa caccagcaa 
      gtactgcga gtacgacaa cttcgccac cagggcgaa 
      acccctagt caggaccaa aatcgaaaa ctcacaaca 
      gtacatact cttcctcaa cacctccgg gccgcacaa 
      gttctccgc cgcttttgt accggaacg atacacaag 
      caaaatgga aagagccat cgtcaaagt cactaatga 
      taactttac tccgaagcc tgtcaggat caacgagag 
      cgcctgcca cgccaggca ccgaaaata cacgtgtag 
      agtcgaggg attcccctt gcccacagt ccagtggaa 
      gcacaacaa cacgatcct ccacaacag ggaccgcgt 
      ccgcatcat tcagaaaaa gcgaaaaag tattctggt 
      ggtcgacaa acgctcgga tacgggaca gtacacctg 
      tgtggcgat tgcagcgac tggagagga ggtttccta 
      tgtcgtccg ccgcaactc gtcgccgtc gaagtccaa 
      gacacacaa ctccaagca caaaatgca ctcgacctc 
      caggcccaa gacgcacag gatcttcag gcccaacac 
      acacacgac ctccgggcc tttacagac acctggaga 
      accctgcaa ggaagacat gcgagagaa cttttgtca 
      taatggggg cacttgctt tgccacgag aatcacagg 
      cggcaaagt accgaattc ctatgattg tgtgtgtcc 
      tcccgggta cacgggtca tagatgtga ggggaaaat 
      gtccgatca atccagcaa aaaagcgac cgattcgta 
      ttgtgaggg agcggaaaa gagcgacaa ggctggata 
      accctatac caaagaaaa ttgcattac agaggatgt 
      agatactcg tttaattta tttgatttg gagaaaaac 
      gcacaatgt agtagtcga tgtgtaata attttttta 
      tcatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY