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Gene information
General information
TC014556 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11675161..11677771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014556
    iBeetle screen iB_05650

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014556 RA
      MSGASISYP HQHGFSSEL KCEAQSRTD 
      QRSGNIAMA TCYPAYELT PPQRTVQVP 
      VQKDYSRPL HVDCSVEYE LPNTAKPPQ 
      GVRNEPLLM IHPCYFRKI ESQRRSPFV 
      NNLPSRQPQ PAVAAKRRA RPQPAQPQQ 
      AAQYPVQYA ADRQQLWDQ MPNLVPISQ 
      AYCKRDQLA YSGDSGHWT DPDRSPDRP 
      EDKHILSGK YRQYLRAHR LHPYVTSAI 
      PLASTFPQI QQVCYNV
    CDS sequence
    • >TC014556 RA
      atgtcggga gcatcgatt tcctacccc caccagcac 
      gggttctcc tcggagctg aagtgcgaa gctcagtcg 
      aggaccgat cagcggtca ggaaacatc gcgatggcg 
      acgtgttac ccagcgtat gagttgact cctcctcag 
      aggacggtg caagtgcct gtgcagaag gactattcg 
      aggccgctg cacgtcgac tgcagcgtc gagtacgag 
      ctgcccaac acggccaag cccccgcag ggcgtccgc 
      aacgagccg ctgctgatg atccacccc tgctacttc 
      cgcaagatc gagagccag cggaggagc cccttcgtc 
      aacaacctg ccctcgcgg cagccgcag cccgccgtc 
      gcggccaag cggcgcgcc cgcccgcag cccgcgcag 
      ccccagcag gccgcccag tacccggtg cagtacgcc 
      gccgaccgc cagcagctc tgggaccag atgcccaac 
      ctggtgccc atctcgcag gcctactgc aagcgcgac 
      cagctggcc tactccggc gactccggc cactggacg 
      gacccggac cgcagcccg gaccggccc gaggacaag 
      cacatcctc tccggcaag taccggcag tacttgcgg 
      gctcacaga ctgcacccc tatgtgact tctgccata 
      ccgctggcc tctaccttc ccgcagatc cagcaggtg 
      tgctacaac gtgtga
    mRNA sequence
    • >TC014556 RA
      actcgtaga cagtcagaa tgtcgggag catcgattt 
      cctaccccc accagcacg ggttctcct cggagctga 
      agtgcgaag ctcagtcga ggaccgatc agcggtcag 
      gaaacatcg cgatggcga cgtgttacc cagcgtatg 
      agttgactc ctcctcaga ggacggtgc aagtgcctg 
      tgcagaagg actattcga ggccgctgc acgtcgact 
      gcagcgtcg agtacgagc tgcccaaca cggccaagc 
      ccccgcagg gcgtccgca acgagccgc tgctgatga 
      tccacccct gctacttcc gcaagatcg agagccagc 
      ggaggagcc ccttcgtca acaacctgc cctcgcggc 
      agccgcagc ccgccgtcg cggccaagc ggcgcgccc 
      gcccgcagc ccgcgcagc cccagcagg ccgcccagt 
      acccggtgc agtacgccg ccgaccgcc agcagctct 
      gggaccaga tgcccaacc tggtgccca tctcgcagg 
      cctactgca agcgcgacc agctggcct actccggcg 
      actccggcc actggacgg acccggacc gcagcccgg 
      accggcccg aggacaagc acatcctct ccggcaagt 
      accggcagt acttgcggg ctcacagac tgcacccct 
      atgtgactt ctgccatac cgctggcct ctaccttcc 
      cgcagatcc agcaggtgt gctacaacg tgtgatcgc 
      tgtgatatg atttcgacg acttttgta cggatcctc 
      tcgttgcat ctatggtgt ccgtaaccc ttcttgtga 
      taatagtgt gtaattctg tgacggaga tgaaagagc 
      gcaagcgtc gtgctccgg gcgtggtag tgattcctc 
      cacatcagc tgtgtttgt gcaactgcg acctaaccg 
      ccgtctatt tggatttct cgttctctt ttggattat 
      cctgtctcg ccgccaatt gcgttactg ttgctaagg 
      atgcccacc cgtttgcga ctcgatcct tccgaacac 
      cgaaaccca attcgcgaa tttcgagca aaaccgcca 
      ccaaaccaa actctaaat ctcccccat cagctgatt 
      ccaccacag tgtcaatgg ctagctgtc atagtcatg 
      caaagcttc tcactactg tacctaatt agagaggtt 
      tagagctga tccgtgttt ggtagacaa cctcaatgc 
      catatttaa aatcctaat ttagtttat aaggtaatt 
      ttattctgt acatattcg tgttgctgt tttgaaaag 
      ttgtaaatt tcgtgtccc aattagccc cattcagtg 
      attatttat gaaacgtaa atgcaatag catcacgca 
      tcttccgaa tttatagcc gacttattt tatcgcgta 
      atttaaaaa aatcaatag acgggatcg tgtaaatcg 
      agccgaaca ggaaaaagt aaatagagc agataacgc 
      aagaactga catcgtggt ccgcttttg agcggattt 
      gtattaata tcgctgtta tcacacgaa atatttacg 
      ttttatgtt gattattta gataggaag ttaaatatt 
      atttcctgt gcatacagc tgttacgtg taaaattag 
      acaagtcta gcgtaacct tctgtcttt cactagatt 
      tagggttag tgtttgtaa tatacacca agaaattct 
      ccacggcag aatatttac gggttttat cggtgttat 
      agcatatag acaatactt tttctaagg attattttg 
      tacaatggc ttgaactgg acgacgtcg caaacgcct 
      gggttccgc gtttcgtaa caattttcg atgacccta 
      acgttgttt gttgttgaa tcatcagaa gtgaaatta 
      attatgcaa aagcttccg tcggaggga agcgcgcag 
      tcatggaag attgtttag tgattttgg gagtattgc 
      ccccgtaaa tcgactgat ttttttgta attagttcg 
      agggaagtg tagataatt ttgttaccg gattgtaca 
      tttgtatct tttttaagt ttgtaatag tagcattcc 
      gttgatttc catgaacaa tgccaattt agggtcgca 
      attgatgcc atattgaaa aagtgccga gagcatttc 
      atgtaacca ttcgttggg cgattccgg ggatcgctt 
      gggtcctcg gaagggcgc aaccgtttc gtatttatc 
      acagtatac caaataacc taattaact tatgtatgt 
      acaaaatca ggtggcagg actcgccgg cttcaaaat 
      ttcagggta caatcctcg gaaaagaac tgaaaaact 
      attttgaat atttttgac tcgtagatt ttatttttg 
      tagctcgaa ctcgtatca aatcgttcg actagattt 
      atttcgggg gatcttttg ggccggctt gttctcccg 
      catccaata gtatgaaga tctgattaa aacttattc 
      gttctatgg ctactacta gaccaaaga aatatctat 
      caactgtat atataaaag tgaaaaaaa cgatcggtt 
      ccagattgt attgctgcg gccggtttg aaaaaaatt 
      gtgaatatg tataaaata ttgctatat atattgtac 
      agctagtca tagtgaaaa tacgcttat aaaatatga 
      ataaaacgt gttactgtt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY