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Gene information
General information
TC014542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11372241..11394025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014542
    iBeetle screen iB_05648

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014542 RA
      MAAVSNGRS AMDSQSETQ NVVVDRLKR LYPAVNEEK 
      SPLPRAWSP KEKYNYIGL SQNNLRVHY KGHGKTHRD 
      AASVQATHP IPAACGLYY FEVKIVSKG RDGYMGIGL 
      SAQGVNMNR LPGWDKQSY GYHGDDGHS FCSSGTGQP 
      YGPTFTTGD VIGCGVNLI DNTCFYTKN GHHLGTAFT 
      DLPPNLYPT VGLQTPGEV VDANFGQEP FVFDIEDMM 
      RELGTRTRM EINDFPVPE SHGEWQAIL NKMVSTYLV 
      HHGYCNTAE AFAHITEQP FNEDIVSIK NRQKILKLV 
      LAGRMGEAI ERTSRLYPG LLESNQNLL FMLKCRQFV 
      EMVNGSDVE VCHRKSGYS PSYSVSPRP SSPIQTSVI 
      QSTKSYSNK SKQSVEEMN QNNTTPTHN GNSCREVPV 
      ILADDGDVE MDTTEEFRN GHGDACNGK SNSSTNGYQ 
      NGNSHSNNV TQDDGCCDN EDEDMEVDV PVCRKSCSK 
      PAVERILEF GRELYSMSQ RLTVDKNSS ETNQKMLED 
      AFSLLAYSN PWASPVGWQ LQPSQRESV CASLNSAIL 
      ESNKKPRKP PLEVAIAHA HELVKLMST AGLGSCAFA 
      SLVDILDQ
    CDS sequence
    • >TC014542 RA
      atggccgcc gtgagtaat ggtcggagt gcgatggat 
      tctcaatca gaaacgcag aatgtcgtg gtggaccgt 
      ttaaaaaga ctgtatccg gccgtaaac gaggagaag 
      tcgccgttg ccccgcgcc tggagcccc aaggaaaag 
      tacaactac atcggactg tcccagaac aatttacgc 
      gttcattac aaagggcac ggcaaaacc caccgagat 
      gccgcaagc gtccaagcg acgcatccc attcctgcc 
      gcctgcggc ctttactac ttcgaagtg aagatcgta 
      agcaaaggt cgcgacggc tacatgggc atcggtctc 
      tcagcccaa ggcgtgaac atgaaccga ttgccaggc 
      tgggacaaa caatcgtac ggttatcac ggcgacgac 
      ggccattcg ttctgctcg tctggcacc ggccagccg 
      tacggaccg acctttacg acaggagac gtcatcggt 
      tgcggcgtc aacctcatc gataacacg tgcttttac 
      acgaaaaac ggacaccac ctcggtacc gccttcaca 
      gatttgcct ccgaatttg tacccaacg gtgggtctt 
      caaactcct ggagaagtg gtcgatgcg aacttcgga 
      caagaaccg ttcgttttc gacatcgag gacatgatg 
      agggagctg gggacgagg acaagaatg gaaatcaac 
      gattttcca gtaccagaa agtcacggt gaatggcaa 
      gccattctg aacaagatg gtatcaact tatctcgtc 
      catcacggt tattgcaat acagcagag gccttcgcc 
      cacatcaca gaacaacct tttaacgaa gacatagta 
      tcaattaag aatcgacaa aaaattcta aagttagtg 
      ttagccggt agaatgggc gaagcgatc gagcgaacg 
      agccgactg taccccggc ctgttagag tcaaatcag 
      aatctctta tttatgctc aaatgtcgc cagtttgtc 
      gaaatggta aatggttca gacgtggag gtctgtcat 
      cgaaaaagt ggttattcg ccgtcgtat tcggtatcg 
      cctagacct agtagtccc atccaaact tcagttata 
      caatcgact aaaagttat agcaataaa tcgaaacaa 
      agcgttgaa gaaatgaat caaaacaat acaacgccg 
      acacataat ggtaatagt tgtagagaa gttcccgta 
      atactcgca gacgatggc gacgtcgaa atggacacc 
      accgaagag ttccgaaat ggtcacgga gacgcctgc 
      aatggcaag tcaaatagt tcgaccaac gggtaccaa 
      aatggtaat agccatagt aataacgtc actcaggat 
      gacggttgc tgtgataat gaagacgaa gatatggag 
      gttgatgtg ccagtgtgc cggaagtca tgttcaaag 
      ccggcagtc gaacgaatt ctagaattt ggtagggaa 
      ttatatagt atgagccaa cgattgaca gtagacaag 
      aattctagt gagacaaat caaaaaatg ttggaagat 
      gcatttagt ttattagca tactcaaac ccatgggcg 
      agtcccgtg ggttggcaa ttgcagccc tcgcaaaga 
      gagtcggtt tgtgcttcc cttaactca gccatttta 
      gaatcaaat aaaaagccc cgaaaaccg cctttagaa 
      gtcgcaatc gcgcacgcc catgaactg gtaaagctg 
      atgtccacg gcgggactc ggctcgtgt gccttcgcc 
      tcgttagtc gacatcttg gatcagtga
    mRNA sequence
    • >TC014542 RA
      taagtcagt acagattcg gcgtcgctg ttaccacag 
      caacgaaat cgcgttgcg cgtagtaac caaaattgt 
      gacatctat cagctgagc gtgtaagct ctttggtgg 
      cgctgtccg agtatgcgc ttcgtggtt cccctacag 
      aagggaaag ggagtttag aaatggccg ccgtgagta 
      atggtcgga gtgcgatgg attctcaat cagaaacgc 
      agaatgtcg tggtggacc gtttaaaaa gactgtatc 
      cggccgtaa acgaggaga agtcgccgt tgccccgcg 
      cctggagcc ccaaggaaa agtacaact acatcggac 
      tgtcccaga acaatttac gcgttcatt acaaagggc 
      acggcaaaa cccaccgag atgccgcaa gcgtccaag 
      cgacgcatc ccattcctg ccgcctgcg gcctttact 
      acttcgaag tgaagatcg taagcaaag gtcgcgacg 
      gctacatgg gcatcggtc tctcagccc aaggcgtga 
      acatgaacc gattgccag gctgggaca aacaatcgt 
      acggttatc acggcgacg acggccatt cgttctgct 
      cgtctggca ccggccagc cgtacggac cgaccttta 
      cgacaggag acgtcatcg gttgcggcg tcaacctca 
      tcgataaca cgtgctttt acacgaaaa acggacacc 
      acctcggta ccgccttca cagatttgc ctccgaatt 
      tgtacccaa cggtgggtc ttcaaactc ctggagaag 
      tggtcgatg cgaacttcg gacaagaac cgttcgttt 
      tcgacatcg aggacatga tgagggagc tggggacga 
      ggacaagaa tggaaatca acgattttc cagtaccag 
      aaagtcacg gtgaatggc aagccattc tgaacaaga 
      tggtatcaa cttatctcg tccatcacg gttattgca 
      atacagcag aggccttcg cccacatca cagaacaac 
      cttttaacg aagacatag tatcaatta agaatcgac 
      aaaaaattc taaagttag tgttagccg gtagaatgg 
      gcgaagcga tcgagcgaa cgagccgac tgtaccccg 
      gcctgttag agtcaaatc agaatctct tatttatgc 
      tcaaatgtc gccagtttg tcgaaatgg taaatggtt 
      cagacgtgg aggtctgtc atcgaaaaa gtggttatt 
      cgccgtcgt attcggtat cgcctagac ctagtagtc 
      ccatccaaa cttcagtta tacaatcga ctaaaagtt 
      atagcaata aatcgaaac aaagcgttg aagaaatga 
      atcaaaaca atacaacgc cgacacata atggtaata 
      gttgtagag aagttcccg taatactcg cagacgatg 
      gcgacgtcg aaatggaca ccaccgaag agttccgaa 
      atggtcacg gagacgcct gcaatggca agtcaaata 
      gttcgacca acgggtacc aaaatggta atagccata 
      gtaataacg tcactcagg atgacggtt gctgtgata 
      atgaagacg aagatatgg aggttgatg tgccagtgt 
      gccggaagt catgttcaa agccggcag tcgaacgaa 
      ttctagaat ttggtaggg aattatata gtatgagcc 
      aacgattga cagtagaca agaattcta gtgagacaa 
      atcaaaaaa tgttggaag atgcattta gtttattag 
      catactcaa acccatggg cgagtcccg tgggttggc 
      aattgcagc cctcgcaaa gagagtcgg tttgtgctt 
      cccttaact cagccattt tagaatcaa ataaaaagc 
      cccgaaaac cgcctttag aagtcgcaa tcgcgcacg 
      cccatgaac tggtaaagc tgatgtcca cggcgggac 
      tcggctcgt gtgccttcg cctcgttag tcgacatct 
      tggatcagt gacctctct cgtcgccct tctgcatta 
      taatgcact gactgccat tctcttcag agagacgat 
      ctaactggt tgcatcatt gtccaagaa gaggaacag 
      cttacttat tcccaatag ttaacagtt gcagttaaa 
      ttatcgcta ttggcagag agaatcgat ggtcggttt 
      agcaaacgg atggcgatc agtttcaat cacaaaatg 
      tggatcaca attcaaacc aataaatta ctgataatt 
      gtgtcgttt tacttcgcc gcttattgg agagagttt 
      atttcgtat cacaatcgg ttagagagg aagtcgtgt 
      attttccgt tctggattt atttgtttg ttatttggt 
      tttatgtct ttgattggc taatttaag gaaacctta 
      cattttata tgttttgtt gtagtttta ttaatttaa 
      cgtaaataa acataaagt cacttaaca aataatcta 
      atctgagtt ctagatgat tttgttgtt gtttggggc 
      gttaagaat tttttgcca agagactac gaggtgttt 
      ttatcaaat ttttgaaga ggtgtagac ataatgcgt 
      agtttagtt tatttgtaa atatgttat acataatta 
      tgttattac tcaaataat aaactaata tttaattat 
      atttttttt cgtttgctt ttccgggtt atcctttgt 
      tgcgtgctt tcgagattt cctctctgc gtgcgctga 
      aaatcatat cagaaacca acgttatga gtaaaaacc 
      tacaactta aggaggaag ttataataa agcatttgt 
      ggtagattg ttatttagt gaaattatc aggttgaaa 
      ataggatat aaataatat acaagataa atacaaact 
      atcagctgt attccaatg taattaagt gatttaaaa 
      tcgtgcact aacactagc ttgtcctca atattataa 
      tacctaatt gtgataaat tcttgtctt gtaaagatt 
      gtgtacgtg cagtgatgt cttgtgaaa agttaatca 
      tgtagatgg cgttgtttt actattttc aaactatgg 
      gaaaatgtt ataaagaaa ataaagatg ataatcgta 
      a
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