General information | |||
TC014542 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 11372241..11394025 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014542 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05648 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC014542 RA
MAAVSNGRS AMDSQSETQ NVVVDRLKR LYPAVNEEK SPLPRAWSP KEKYNYIGL SQNNLRVHY KGHGKTHRD AASVQATHP IPAACGLYY FEVKIVSKG RDGYMGIGL SAQGVNMNR LPGWDKQSY GYHGDDGHS FCSSGTGQP YGPTFTTGD VIGCGVNLI DNTCFYTKN GHHLGTAFT DLPPNLYPT VGLQTPGEV VDANFGQEP FVFDIEDMM RELGTRTRM EINDFPVPE SHGEWQAIL NKMVSTYLV HHGYCNTAE AFAHITEQP FNEDIVSIK NRQKILKLV LAGRMGEAI ERTSRLYPG LLESNQNLL FMLKCRQFV EMVNGSDVE VCHRKSGYS PSYSVSPRP SSPIQTSVI QSTKSYSNK SKQSVEEMN QNNTTPTHN GNSCREVPV ILADDGDVE MDTTEEFRN GHGDACNGK SNSSTNGYQ NGNSHSNNV TQDDGCCDN EDEDMEVDV PVCRKSCSK PAVERILEF GRELYSMSQ RLTVDKNSS ETNQKMLED AFSLLAYSN PWASPVGWQ LQPSQRESV CASLNSAIL ESNKKPRKP PLEVAIAHA HELVKLMST AGLGSCAFA SLVDILDQ
- >TC014542 RA
atggccgcc gtgagtaat ggtcggagt gcgatggat tctcaatca gaaacgcag aatgtcgtg gtggaccgt ttaaaaaga ctgtatccg gccgtaaac gaggagaag tcgccgttg ccccgcgcc tggagcccc aaggaaaag tacaactac atcggactg tcccagaac aatttacgc gttcattac aaagggcac ggcaaaacc caccgagat gccgcaagc gtccaagcg acgcatccc attcctgcc gcctgcggc ctttactac ttcgaagtg aagatcgta agcaaaggt cgcgacggc tacatgggc atcggtctc tcagcccaa ggcgtgaac atgaaccga ttgccaggc tgggacaaa caatcgtac ggttatcac ggcgacgac ggccattcg ttctgctcg tctggcacc ggccagccg tacggaccg acctttacg acaggagac gtcatcggt tgcggcgtc aacctcatc gataacacg tgcttttac acgaaaaac ggacaccac ctcggtacc gccttcaca gatttgcct ccgaatttg tacccaacg gtgggtctt caaactcct ggagaagtg gtcgatgcg aacttcgga caagaaccg ttcgttttc gacatcgag gacatgatg agggagctg gggacgagg acaagaatg gaaatcaac gattttcca gtaccagaa agtcacggt gaatggcaa gccattctg aacaagatg gtatcaact tatctcgtc catcacggt tattgcaat acagcagag gccttcgcc cacatcaca gaacaacct tttaacgaa gacatagta tcaattaag aatcgacaa aaaattcta aagttagtg ttagccggt agaatgggc gaagcgatc gagcgaacg agccgactg taccccggc ctgttagag tcaaatcag aatctctta tttatgctc aaatgtcgc cagtttgtc gaaatggta aatggttca gacgtggag gtctgtcat cgaaaaagt ggttattcg ccgtcgtat tcggtatcg cctagacct agtagtccc atccaaact tcagttata caatcgact aaaagttat agcaataaa tcgaaacaa agcgttgaa gaaatgaat caaaacaat acaacgccg acacataat ggtaatagt tgtagagaa gttcccgta atactcgca gacgatggc gacgtcgaa atggacacc accgaagag ttccgaaat ggtcacgga gacgcctgc aatggcaag tcaaatagt tcgaccaac gggtaccaa aatggtaat agccatagt aataacgtc actcaggat gacggttgc tgtgataat gaagacgaa gatatggag gttgatgtg ccagtgtgc cggaagtca tgttcaaag ccggcagtc gaacgaatt ctagaattt ggtagggaa ttatatagt atgagccaa cgattgaca gtagacaag aattctagt gagacaaat caaaaaatg ttggaagat gcatttagt ttattagca tactcaaac ccatgggcg agtcccgtg ggttggcaa ttgcagccc tcgcaaaga gagtcggtt tgtgcttcc cttaactca gccatttta gaatcaaat aaaaagccc cgaaaaccg cctttagaa gtcgcaatc gcgcacgcc catgaactg gtaaagctg atgtccacg gcgggactc ggctcgtgt gccttcgcc tcgttagtc gacatcttg gatcagtga
- >TC014542 RA
taagtcagt acagattcg gcgtcgctg ttaccacag caacgaaat cgcgttgcg cgtagtaac caaaattgt gacatctat cagctgagc gtgtaagct ctttggtgg cgctgtccg agtatgcgc ttcgtggtt cccctacag aagggaaag ggagtttag aaatggccg ccgtgagta atggtcgga gtgcgatgg attctcaat cagaaacgc agaatgtcg tggtggacc gtttaaaaa gactgtatc cggccgtaa acgaggaga agtcgccgt tgccccgcg cctggagcc ccaaggaaa agtacaact acatcggac tgtcccaga acaatttac gcgttcatt acaaagggc acggcaaaa cccaccgag atgccgcaa gcgtccaag cgacgcatc ccattcctg ccgcctgcg gcctttact acttcgaag tgaagatcg taagcaaag gtcgcgacg gctacatgg gcatcggtc tctcagccc aaggcgtga acatgaacc gattgccag gctgggaca aacaatcgt acggttatc acggcgacg acggccatt cgttctgct cgtctggca ccggccagc cgtacggac cgaccttta cgacaggag acgtcatcg gttgcggcg tcaacctca tcgataaca cgtgctttt acacgaaaa acggacacc acctcggta ccgccttca cagatttgc ctccgaatt tgtacccaa cggtgggtc ttcaaactc ctggagaag tggtcgatg cgaacttcg gacaagaac cgttcgttt tcgacatcg aggacatga tgagggagc tggggacga ggacaagaa tggaaatca acgattttc cagtaccag aaagtcacg gtgaatggc aagccattc tgaacaaga tggtatcaa cttatctcg tccatcacg gttattgca atacagcag aggccttcg cccacatca cagaacaac cttttaacg aagacatag tatcaatta agaatcgac aaaaaattc taaagttag tgttagccg gtagaatgg gcgaagcga tcgagcgaa cgagccgac tgtaccccg gcctgttag agtcaaatc agaatctct tatttatgc tcaaatgtc gccagtttg tcgaaatgg taaatggtt cagacgtgg aggtctgtc atcgaaaaa gtggttatt cgccgtcgt attcggtat cgcctagac ctagtagtc ccatccaaa cttcagtta tacaatcga ctaaaagtt atagcaata aatcgaaac aaagcgttg aagaaatga atcaaaaca atacaacgc cgacacata atggtaata gttgtagag aagttcccg taatactcg cagacgatg gcgacgtcg aaatggaca ccaccgaag agttccgaa atggtcacg gagacgcct gcaatggca agtcaaata gttcgacca acgggtacc aaaatggta atagccata gtaataacg tcactcagg atgacggtt gctgtgata atgaagacg aagatatgg aggttgatg tgccagtgt gccggaagt catgttcaa agccggcag tcgaacgaa ttctagaat ttggtaggg aattatata gtatgagcc aacgattga cagtagaca agaattcta gtgagacaa atcaaaaaa tgttggaag atgcattta gtttattag catactcaa acccatggg cgagtcccg tgggttggc aattgcagc cctcgcaaa gagagtcgg tttgtgctt cccttaact cagccattt tagaatcaa ataaaaagc cccgaaaac cgcctttag aagtcgcaa tcgcgcacg cccatgaac tggtaaagc tgatgtcca cggcgggac tcggctcgt gtgccttcg cctcgttag tcgacatct tggatcagt gacctctct cgtcgccct tctgcatta taatgcact gactgccat tctcttcag agagacgat ctaactggt tgcatcatt gtccaagaa gaggaacag cttacttat tcccaatag ttaacagtt gcagttaaa ttatcgcta ttggcagag agaatcgat ggtcggttt agcaaacgg atggcgatc agtttcaat cacaaaatg tggatcaca attcaaacc aataaatta ctgataatt gtgtcgttt tacttcgcc gcttattgg agagagttt atttcgtat cacaatcgg ttagagagg aagtcgtgt attttccgt tctggattt atttgtttg ttatttggt tttatgtct ttgattggc taatttaag gaaacctta cattttata tgttttgtt gtagtttta ttaatttaa cgtaaataa acataaagt cacttaaca aataatcta atctgagtt ctagatgat tttgttgtt gtttggggc gttaagaat tttttgcca agagactac gaggtgttt ttatcaaat ttttgaaga ggtgtagac ataatgcgt agtttagtt tatttgtaa atatgttat acataatta tgttattac tcaaataat aaactaata tttaattat atttttttt cgtttgctt ttccgggtt atcctttgt tgcgtgctt tcgagattt cctctctgc gtgcgctga aaatcatat cagaaacca acgttatga gtaaaaacc tacaactta aggaggaag ttataataa agcatttgt ggtagattg ttatttagt gaaattatc aggttgaaa ataggatat aaataatat acaagataa atacaaact atcagctgt attccaatg taattaagt gatttaaaa tcgtgcact aacactagc ttgtcctca atattataa tacctaatt gtgataaat tcttgtctt gtaaagatt gtgtacgtg cagtgatgt cttgtgaaa agttaatca tgtagatgg cgttgtttt actattttc aaactatgg gaaaatgtt ataaagaaa ataaagatg ataatcgta a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
