Status
Please wait ...
Gene information
General information
TC014541 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11363301..11368995
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014541
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: In few cases, labrum, maxilla or labium are missing.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014541 RA
      MSEAEATTS TTDSTEPEK ITEDLLEPE AKRRKTAAI 
      QQDEKKYRL EERLGGILC CAVCLDLPK AAVYQCSNG 
      HLMCAGCFT HILADARLR DETSTCPTC RVEICKTLA 
      TRNLAVENA VSELPSECQ FCNKQFPRN SLEKHEDEE 
      CEDRISGCK YHRIGCPWR GPVHERAQH EKECGHPHK 
      SGAEVMEAL EILDQQLIE ERKLYKTIF ELLGYDKIT 
      FNDIQLKPY RTDDFVHRL FYESSRFSA FNNQWVVKA 
      KISDNQKDP TQSSERDMT YQLILKSKT STPLSLSYI 
      VLRGPVGDV KINPRVYNF DFTEQETET PYEHFPLPD 
      TAECNRLLA AKTINFRLI MFLASK
    • >TC014541 RB
      MSEAEATTS TTDSTEPEK ITEDLLEPE AKRRKTAAI 
      QQDEKKYRL EERLGGILC CAVCLDLPK AAVYQVSAF 
      ASCRFP
    • >TC014541 RC
      MCAGCFTHI LADARLRDE TSTCPTCRV EICKTLATR 
      NLAVENAVS ELPSECQFC NKQFPRNSL EKHEDEECE 
      DRISGCKYH RIGCPWRGP VHERAQHEK ECGHPHKSG 
      AEVMEALEI LDQQLIEER KLYKTIFEL LGYDKITFN 
      DIQLKPYRT DDFVHRLFY ESSRFSAFN NQWVVKAKI 
      SDNQKDPTQ SSERDMTYQ LILKSKTST PLSLSYIVL 
      RGPVGDVKI NPRVYNFDF TEQETETPY EHFPLPDTA 
      ECNRLLAAK TINFRLIMF LASK
    CDS sequence
    • >TC014541 RA
      atgtcggaa gctgaggcg acgacgtcc accacggac 
      tccacggag ccggagaag atcactgaa gacctgctc 
      gagccggag gcgaaacgt cgcaaaacc gccgccata 
      cagcaggac gaaaagaag tacaggctc gaggagagg 
      ctgggagga attctctgc tgcgcagtt tgcctagac 
      ctgcccaaa gccgccgtt taccagtgc agcaatggt 
      cacttgatg tgtgccgga tgtttcacg cacatcctc 
      gccgatgcc cgtttgcgt gacgaaacg tcaacatgt 
      cccacttgc cgcgtggaa atctgcaag acattggcg 
      acgagaaat ttggctgtt gaaaacgcc gtctcggaa 
      ctaccctcc gaatgtcag ttctgcaac aagcagttt 
      ccgaggaat tccttggaa aagcacgaa gacgaggaa 
      tgcgaggac agaatttca ggttgcaag taccacagg 
      atcgggtgt ccgtggaga ggaccagtg catgaaagg 
      gcgcaacat gaaaaagaa tgcggacat ccgcataag 
      tctggggcg gaagttatg gaggctttg gaaatactc 
      gatcagcag ctgattgaa gaaagaaaa ctatacaag 
      acgatattt gagttgttg ggttacgat aaaattact 
      tttaacgat attcagttg aagccgtat cgtaccgac 
      gattttgtc catcgatta ttttatgaa tcatcgcga 
      ttttcggcc ttcaataat cagtgggta gtcaaagct 
      aaaattagt gataatcag aaggacccc acacaaagc 
      tcagaacga gatatgact tatcagtta atcctgaaa 
      tcaaaaaca tctaccccc ttgagtttg agttacatc 
      gtcttacga ggtcctgtc ggagatgtg aagataaat 
      cctcgagtc tacaatttc gatttcaca gaacaagaa 
      accgaaact ccctacgaa cactttccc ctaccagac 
      acagctgaa tgtaatcgt ttactagca gcaaaaaca 
      attaatttc aggttaatc atgtttttg gcctcaaaa 
      taa
    • >TC014541 RB
      atgtcggaa gctgaggcg acgacgtcc accacggac 
      tccacggag ccggagaag atcactgaa gacctgctc 
      gagccggag gcgaaacgt cgcaaaacc gccgccata 
      cagcaggac gaaaagaag tacaggctc gaggagagg 
      ctgggagga attctctgc tgcgcagtt tgcctagac 
      ctgcccaaa gccgccgtt taccaggta agtgccttt 
      gcctcctgc agatttccc tag
    • >TC014541 RC
      atgtgtgcc ggatgtttc acgcacatc ctcgccgat 
      gcccgtttg cgtgacgaa acgtcaaca tgtcccact 
      tgccgcgtg gaaatctgc aagacattg gcgacgaga 
      aatttggct gttgaaaac gccgtctcg gaactaccc 
      tccgaatgt cagttctgc aacaagcag tttccgagg 
      aattccttg gaaaagcac gaagacgag gaatgcgag 
      gacagaatt tcaggttgc aagtaccac aggatcggg 
      tgtccgtgg agaggacca gtgcatgaa agggcgcaa 
      catgaaaaa gaatgcgga catccgcat aagtctggg 
      gcggaagtt atggaggct ttggaaata ctcgatcag 
      cagctgatt gaagaaaga aaactatac aagacgata 
      tttgagttg ttgggttac gataaaatt acttttaac 
      gatattcag ttgaagccg tatcgtacc gacgatttt 
      gtccatcga ttattttat gaatcatcg cgattttcg 
      gccttcaat aatcagtgg gtagtcaaa gctaaaatt 
      agtgataat cagaaggac cccacacaa agctcagaa 
      cgagatatg acttatcag ttaatcctg aaatcaaaa 
      acatctacc cccttgagt ttgagttac atcgtctta 
      cgaggtcct gtcggagat gtgaagata aatcctcga 
      gtctacaat ttcgatttc acagaacaa gaaaccgaa 
      actccctac gaacacttt cccctacca gacacagct 
      gaatgtaat cgtttacta gcagcaaaa acaattaat 
      ttcaggtta atcatgttt ttggcctca aaataa
    mRNA sequence
    • >TC014541 RA
      acgcataca ttagaaaaa tttgcacag acttcctgg 
      tccaagtcg ttatcagca tcaaaatag atttttgtc 
      caataaacc taaattatt ccgtcaaca taataaccc 
      gagataaac ataatcagc tttaatctc cgccgacca 
      ttaagtaaa acgtaaaca ccatgcgta ttaccataa 
      acaacccaa cgcaaggtt tctaatgtt ttggtcggc 
      tctagacca tcagctgat tcacctgag cccaccatt 
      ctttgttgt ccaaaccag cagctgact gtcatcccc 
      tccacaccg cgtacgtca caccccagc cggccaatc 
      agaccccga gctggggca ttccgtggt ggggagggc 
      atgtcaaac tgaggcgtc acgtcagct gttttctgg 
      ttatgtgcg aggtgtaga ggtagacag gaagtcagc 
      tgttgtgtg tttaacttg gaacgcatt caccacaat 
      gtcggaagc tgaggcgac gacgtccac cacggactc 
      cacggagcc ggagaagat cactgaaga cctgctcga 
      gccggaggc gaaacgtcg caaaaccgc cgccataca 
      gcaggacga aaagaagta caggctcga ggagaggct 
      gggaggaat tctctgctg cgcagtttg cctagacct 
      gcccaaagc cgccgttta ccagtgcag caatggtca 
      cttgatgtg tgccggatg tttcacgca catcctcgc 
      cgatgcccg tttgcgtga cgaaacgtc aacatgtcc 
      cacttgccg cgtggaaat ctgcaagac attggcgac 
      gagaaattt ggctgttga aaacgccgt ctcggaact 
      accctccga atgtcagtt ctgcaacaa gcagtttcc 
      gaggaattc cttggaaaa gcacgaaga cgaggaatg 
      cgaggacag aatttcagg ttgcaagta ccacaggat 
      cgggtgtcc gtggagagg accagtgca tgaaagggc 
      gcaacatga aaaagaatg cggacatcc gcataagtc 
      tggggcgga agttatgga ggctttgga aatactcga 
      tcagcagct gattgaaga aagaaaact atacaagac 
      gatatttga gttgttggg ttacgataa aattacttt 
      taacgatat tcagttgaa gccgtatcg taccgacga 
      ttttgtcca tcgattatt ttatgaatc atcgcgatt 
      ttcggcctt caataatca gtgggtagt caaagctaa 
      aattagtga taatcagaa ggaccccac acaaagctc 
      agaacgaga tatgactta tcagttaat cctgaaatc 
      aaaaacatc taccccctt gagtttgag ttacatcgt 
      cttacgagg tcctgtcgg agatgtgaa gataaatcc 
      tcgagtcta caatttcga tttcacaga acaagaaac 
      cgaaactcc ctacgaaca ctttcccct accagacac 
      agctgaatg taatcgttt actagcagc aaaaacaat 
      taatttcag gttaatcat gtttttggc ctcaaaata 
      agttatttt cgcatcgtc gcacccaaa attctagtt 
      cgtatttac ttattctga ccctcattt tcttttttt 
      ttttttggc tgaatatac tggaatgtc tatttaaaa 
      gaagtttgt attttgatt aagttagct gaagatctc 
      tatagagtt taggagttt ttatattta gatctttat 
      tttatacat tttatatat ttttggatt gtgagatgt 
      ttttcgttt tacaataac atttgtttt gttaccaac 
      atttgtgtg ttgccatta tttttagtt taatttttt 
      tttggaaac gttttaata acattgtgt cattttatt 
      taactaatg gcttttgtc actgtcatt ctcagcatt 
      ataaaggta gcatagaat aaattaatt tatatattc 
      ttaattgta agaaaaggg atattggta gaaattaa
    • >TC014541 RB
      acgcataca ttagaaaaa tttgcacag acttcctgg 
      tccaagtcg ttatcagca tcaaaatag atttttgtc 
      caataaacc taaattatt ccgtcaaca taataaccc 
      gagataaac ataatcagc tttaatctc cgccgacca 
      ttaagtaaa acgtaaaca ccatgcgta ttaccataa 
      acaacccaa cgcaaggtt tctaatgtt ttggtcggc 
      tctagacca tcagctgat tcacctgag cccaccatt 
      ctttgttgt ccaaaccag cagctgact gtcatcccc 
      tccacaccg cgtacgtca caccccagc cggccaatc 
      agaccccga gctggggca ttccgtggt ggggagggc 
      atgtcaaac tgaggcgtc acgtcagct gttttctgg 
      ttatgtgcg aggtgtaga ggtagacag gaagtcagc 
      tgttgtgtg tttaacttg gaacgcatt caccacaat 
      gtcggaagc tgaggcgac gacgtccac cacggactc 
      cacggagcc ggagaagat cactgaaga cctgctcga 
      gccggaggc gaaacgtcg caaaaccgc cgccataca 
      gcaggacga aaagaagta caggctcga ggagaggct 
      gggaggaat tctctgctg cgcagtttg cctagacct 
      gcccaaagc cgccgttta ccaggtaag tgcctttgc 
      ctcctgcag atttcccta gattacgta acactacgt 
      ctttgtccc gttccgctg cgctcctgt attgatttt 
      caggacact gcggctata tttaaacgc gttcaaggg 
      ccgaggctc ggggtctcc gctcgacct ccattatgc 
      tttatcgtg gttttgcgc acaattttt accaattat 
      tgtgtgcca aaccatggg tttatctca tctactctt 
      tttgtttta ccacatttg tctgcgtca tcctcagtt 
      tgtaattaa atactattg tactttttt cattgttac 
      aatgtagaa tagtcattt tttgtaact gtgatactt 
      aaataataa gactctgga tgtgataat tgataacta 
      ctttaacga gttcttcta ttttataac aacgatgat 
      ttttggttc gacgttgca aataaatgt attattaaa 
      tttatattt tagtcatta ttcatattt agatatagt 
      atttatttt ccccatcct gtcccattt ctttgttgg 
      tttcgaaaa gagcacaag atttttatt taaccacga 
      aataagaga ggtaggtag cgacgacca aggctgggt 
      tactagtaa tttttacaa gtcacgaaa tggcaccac 
      tttcgatta acatttaac cctaagtta atactcttt 
      ggaaaacac attttccat acgatttaa cttgaaaag 
      ttaaagtta accataagc ggtcacgcc acttaaaaa 
      gttcaatca atgctattt gaacaatgg tagagaagg 
      taacgtaat ggtaaaata aagtagcac caccagggg 
      cggatccag aattttcat cagggggtg aaatgtgag 
      cctggataa tccaccata aaacgtaaa tttttttga 
      atgaaagaa ttactgccc tttcagaaa tctgaaata 
      ttttcctca caatgcaat aagtttaaa tttttagag 
      gttcagtta ttaaaatcg taactggtc caatttgtt 
      ctacaaaac gtttctcaa ttattaatt attgccaag 
      aaataaatt tgtagattt taggtccaa gtcgttatc 
      agcataata gatttgtcc aacaaacct aaattattc 
      cgccaacat aataaccct tgataaaca taatcagct 
      ttaatttcc accgaccat taaacattt ttgatttta
    • >TC014541 RC
      actcaaaag aaaatttgg taacttggt tttttactg 
      atgtgacta aatttacaa aattgaatt atatattta 
      agtgatttc aactagttt cgaaaaggc tgccgtgag 
      attataaga aacaagtag aagtgttgt taaatgaaa 
      ccaaagtgt agttggtga agcgtattg ggggttttg 
      gatccggcc ctggcgcac cgccgctct cactcaatg 
      tggtcagtt ggcagaggg taaaaattt gagttagag 
      cggtggcga gtatattcg ttgagtaca tacagcctt 
      attgataaa atttatgtt agttccagg atatgaaga 
      ggattggta agtgtctcc aaagcttaa ttaatgtct 
      gtggggtgg gaacaaaac atttcgaac aatttcaca 
      caaaaaact ttttttgaa ggatgagac tgcagcaat 
      ggtcacttg atgtgtgcc ggatgtttc acgcacatc 
      ctcgccgat gcccgtttg cgtgacgaa acgtcaaca 
      tgtcccact tgccgcgtg gaaatctgc aagacattg 
      gcgacgaga aatttggct gttgaaaac gccgtctcg 
      gaactaccc tccgaatgt cagttctgc aacaagcag 
      tttccgagg aattccttg gaaaagcac gaagacgag 
      gaatgcgag gacagaatt tcaggttgc aagtaccac 
      aggatcggg tgtccgtgg agaggacca gtgcatgaa 
      agggcgcaa catgaaaaa gaatgcgga catccgcat 
      aagtctggg gcggaagtt atggaggct ttggaaata 
      ctcgatcag cagctgatt gaagaaaga aaactatac 
      aagacgata tttgagttg ttgggttac gataaaatt 
      acttttaac gatattcag ttgaagccg tatcgtacc 
      gacgatttt gtccatcga ttattttat gaatcatcg 
      cgattttcg gccttcaat aatcagtgg gtagtcaaa 
      gctaaaatt agtgataat cagaaggac cccacacaa 
      agctcagaa cgagatatg acttatcag ttaatcctg 
      aaatcaaaa acatctacc cccttgagt ttgagttac 
      atcgtctta cgaggtcct gtcggagat gtgaagata 
      aatcctcga gtctacaat ttcgatttc acagaacaa 
      gaaaccgaa actccctac gaacacttt cccctacca 
      gacacagct gaatgtaat cgtttacta gcagcaaaa 
      acaattaat ttcaggtta atcatgttt ttggcctca 
      aaataagtt attttcgca tcgtcgcac ccaaaattc 
      tagttcgta tttacttat tctgaccct cattttctt 
      ttttttttt ttggctgaa tatactgga atgtctatt 
      taaaagaag tttgtattt tgattaagt tagctgaag 
      atctctata gagtttagg agtttttat atttagatc 
      tttatttta tacatttta tatattttt ggattgtga 
      gatgttttt cgttttaca ataacattt gttttgtta 
      ccaacattt gtgtgttgc cattatttt tagtttaat 
      ttttttttg gaaacgttt taataacat tgtgtcatt 
      ttatttaac taatggctt ttgtcactg tcattctca 
      gcattataa aggtagcat agaataaat taatttata 
      tattcttaa ttgtaagaa aagggatat tggtagaaa 
      ttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY