Status
Please wait ...
Gene information
General information
TC014541 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11363301..11368995
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014541
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: In few cases, labrum, maxilla or labium are missing.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014541 RA
      MSEAEATTS TTDSTEPEK ITEDLLEPE AKRRKTAAI 
QQDEKKYRL EERLGGILC CAVCLDLPK AAVYQCSNG 
HLMCAGCFT HILADARLR DETSTCPTC RVEICKTLA 
TRNLAVENA VSELPSECQ FCNKQFPRN SLEKHEDEE 
CEDRISGCK YHRIGCPWR GPVHERAQH EKECGHPHK 
SGAEVMEAL EILDQQLIE ERKLYKTIF ELLGYDKIT 
FNDIQLKPY RTDDFVHRL FYESSRFSA FNNQWVVKA 
KISDNQKDP TQSSERDMT YQLILKSKT STPLSLSYI 
VLRGPVGDV KINPRVYNF DFTEQETET PYEHFPLPD 
TAECNRLLA AKTINFRLI MFLASK
    • >TC014541 RB
      MSEAEATTS TTDSTEPEK ITEDLLEPE AKRRKTAAI 
QQDEKKYRL EERLGGILC CAVCLDLPK AAVYQVSAF 
ASCRFP
    • >TC014541 RC
      MCAGCFTHI LADARLRDE TSTCPTCRV EICKTLATR 
NLAVENAVS ELPSECQFC NKQFPRNSL EKHEDEECE 
DRISGCKYH RIGCPWRGP VHERAQHEK ECGHPHKSG 
AEVMEALEI LDQQLIEER KLYKTIFEL LGYDKITFN 
DIQLKPYRT DDFVHRLFY ESSRFSAFN NQWVVKAKI 
SDNQKDPTQ SSERDMTYQ LILKSKTST PLSLSYIVL 
RGPVGDVKI NPRVYNFDF TEQETETPY EHFPLPDTA 
ECNRLLAAK TINFRLIMF LASK
    CDS sequence
    • >TC014541 RA
      atgtcggaa gctgaggcg acgacgtcc accacggac 
tccacggag ccggagaag atcactgaa gacctgctc 
gagccggag gcgaaacgt cgcaaaacc gccgccata 
cagcaggac gaaaagaag tacaggctc gaggagagg 
ctgggagga attctctgc tgcgcagtt tgcctagac 
ctgcccaaa gccgccgtt taccagtgc agcaatggt 
cacttgatg tgtgccgga tgtttcacg cacatcctc 
gccgatgcc cgtttgcgt gacgaaacg tcaacatgt 
cccacttgc cgcgtggaa atctgcaag acattggcg 
acgagaaat ttggctgtt gaaaacgcc gtctcggaa 
ctaccctcc gaatgtcag ttctgcaac aagcagttt 
ccgaggaat tccttggaa aagcacgaa gacgaggaa 
tgcgaggac agaatttca ggttgcaag taccacagg 
atcgggtgt ccgtggaga ggaccagtg catgaaagg 
gcgcaacat gaaaaagaa tgcggacat ccgcataag 
tctggggcg gaagttatg gaggctttg gaaatactc 
gatcagcag ctgattgaa gaaagaaaa ctatacaag 
acgatattt gagttgttg ggttacgat aaaattact 
tttaacgat attcagttg aagccgtat cgtaccgac 
gattttgtc catcgatta ttttatgaa tcatcgcga 
ttttcggcc ttcaataat cagtgggta gtcaaagct 
aaaattagt gataatcag aaggacccc acacaaagc 
tcagaacga gatatgact tatcagtta atcctgaaa 
tcaaaaaca tctaccccc ttgagtttg agttacatc 
gtcttacga ggtcctgtc ggagatgtg aagataaat 
cctcgagtc tacaatttc gatttcaca gaacaagaa 
accgaaact ccctacgaa cactttccc ctaccagac 
acagctgaa tgtaatcgt ttactagca gcaaaaaca 
attaatttc aggttaatc atgtttttg gcctcaaaa 
taa
    • >TC014541 RB
      atgtcggaa gctgaggcg acgacgtcc accacggac 
tccacggag ccggagaag atcactgaa gacctgctc 
gagccggag gcgaaacgt cgcaaaacc gccgccata 
cagcaggac gaaaagaag tacaggctc gaggagagg 
ctgggagga attctctgc tgcgcagtt tgcctagac 
ctgcccaaa gccgccgtt taccaggta agtgccttt 
gcctcctgc agatttccc tag
    • >TC014541 RC
      atgtgtgcc ggatgtttc acgcacatc ctcgccgat 
gcccgtttg cgtgacgaa acgtcaaca tgtcccact 
tgccgcgtg gaaatctgc aagacattg gcgacgaga 
aatttggct gttgaaaac gccgtctcg gaactaccc 
tccgaatgt cagttctgc aacaagcag tttccgagg 
aattccttg gaaaagcac gaagacgag gaatgcgag 
gacagaatt tcaggttgc aagtaccac aggatcggg 
tgtccgtgg agaggacca gtgcatgaa agggcgcaa 
catgaaaaa gaatgcgga catccgcat aagtctggg 
gcggaagtt atggaggct ttggaaata ctcgatcag 
cagctgatt gaagaaaga aaactatac aagacgata 
tttgagttg ttgggttac gataaaatt acttttaac 
gatattcag ttgaagccg tatcgtacc gacgatttt 
gtccatcga ttattttat gaatcatcg cgattttcg 
gccttcaat aatcagtgg gtagtcaaa gctaaaatt 
agtgataat cagaaggac cccacacaa agctcagaa 
cgagatatg acttatcag ttaatcctg aaatcaaaa 
acatctacc cccttgagt ttgagttac atcgtctta 
cgaggtcct gtcggagat gtgaagata aatcctcga 
gtctacaat ttcgatttc acagaacaa gaaaccgaa 
actccctac gaacacttt cccctacca gacacagct 
gaatgtaat cgtttacta gcagcaaaa acaattaat 
ttcaggtta atcatgttt ttggcctca aaataa
    mRNA sequence
    • >TC014541 RA
      acgcataca ttagaaaaa tttgcacag acttcctgg 
tccaagtcg ttatcagca tcaaaatag atttttgtc 
caataaacc taaattatt ccgtcaaca taataaccc 
gagataaac ataatcagc tttaatctc cgccgacca 
ttaagtaaa acgtaaaca ccatgcgta ttaccataa 
acaacccaa cgcaaggtt tctaatgtt ttggtcggc 
tctagacca tcagctgat tcacctgag cccaccatt 
ctttgttgt ccaaaccag cagctgact gtcatcccc 
tccacaccg cgtacgtca caccccagc cggccaatc 
agaccccga gctggggca ttccgtggt ggggagggc 
atgtcaaac tgaggcgtc acgtcagct gttttctgg 
ttatgtgcg aggtgtaga ggtagacag gaagtcagc 
tgttgtgtg tttaacttg gaacgcatt caccacaat 
gtcggaagc tgaggcgac gacgtccac cacggactc 
cacggagcc ggagaagat cactgaaga cctgctcga 
gccggaggc gaaacgtcg caaaaccgc cgccataca 
gcaggacga aaagaagta caggctcga ggagaggct 
gggaggaat tctctgctg cgcagtttg cctagacct 
gcccaaagc cgccgttta ccagtgcag caatggtca 
cttgatgtg tgccggatg tttcacgca catcctcgc 
cgatgcccg tttgcgtga cgaaacgtc aacatgtcc 
cacttgccg cgtggaaat ctgcaagac attggcgac 
gagaaattt ggctgttga aaacgccgt ctcggaact 
accctccga atgtcagtt ctgcaacaa gcagtttcc 
gaggaattc cttggaaaa gcacgaaga cgaggaatg 
cgaggacag aatttcagg ttgcaagta ccacaggat 
cgggtgtcc gtggagagg accagtgca tgaaagggc 
gcaacatga aaaagaatg cggacatcc gcataagtc 
tggggcgga agttatgga ggctttgga aatactcga 
tcagcagct gattgaaga aagaaaact atacaagac 
gatatttga gttgttggg ttacgataa aattacttt 
taacgatat tcagttgaa gccgtatcg taccgacga 
ttttgtcca tcgattatt ttatgaatc atcgcgatt 
ttcggcctt caataatca gtgggtagt caaagctaa 
aattagtga taatcagaa ggaccccac acaaagctc 
agaacgaga tatgactta tcagttaat cctgaaatc 
aaaaacatc taccccctt gagtttgag ttacatcgt 
cttacgagg tcctgtcgg agatgtgaa gataaatcc 
tcgagtcta caatttcga tttcacaga acaagaaac 
cgaaactcc ctacgaaca ctttcccct accagacac 
agctgaatg taatcgttt actagcagc aaaaacaat 
taatttcag gttaatcat gtttttggc ctcaaaata 
agttatttt cgcatcgtc gcacccaaa attctagtt 
cgtatttac ttattctga ccctcattt tcttttttt 
ttttttggc tgaatatac tggaatgtc tatttaaaa 
gaagtttgt attttgatt aagttagct gaagatctc 
tatagagtt taggagttt ttatattta gatctttat 
tttatacat tttatatat ttttggatt gtgagatgt 
ttttcgttt tacaataac atttgtttt gttaccaac 
atttgtgtg ttgccatta tttttagtt taatttttt 
tttggaaac gttttaata acattgtgt cattttatt 
taactaatg gcttttgtc actgtcatt ctcagcatt 
ataaaggta gcatagaat aaattaatt tatatattc 
ttaattgta agaaaaggg atattggta gaaattaa
    • >TC014541 RB
      acgcataca ttagaaaaa tttgcacag acttcctgg 
tccaagtcg ttatcagca tcaaaatag atttttgtc 
caataaacc taaattatt ccgtcaaca taataaccc 
gagataaac ataatcagc tttaatctc cgccgacca 
ttaagtaaa acgtaaaca ccatgcgta ttaccataa 
acaacccaa cgcaaggtt tctaatgtt ttggtcggc 
tctagacca tcagctgat tcacctgag cccaccatt 
ctttgttgt ccaaaccag cagctgact gtcatcccc 
tccacaccg cgtacgtca caccccagc cggccaatc 
agaccccga gctggggca ttccgtggt ggggagggc 
atgtcaaac tgaggcgtc acgtcagct gttttctgg 
ttatgtgcg aggtgtaga ggtagacag gaagtcagc 
tgttgtgtg tttaacttg gaacgcatt caccacaat 
gtcggaagc tgaggcgac gacgtccac cacggactc 
cacggagcc ggagaagat cactgaaga cctgctcga 
gccggaggc gaaacgtcg caaaaccgc cgccataca 
gcaggacga aaagaagta caggctcga ggagaggct 
gggaggaat tctctgctg cgcagtttg cctagacct 
gcccaaagc cgccgttta ccaggtaag tgcctttgc 
ctcctgcag atttcccta gattacgta acactacgt 
ctttgtccc gttccgctg cgctcctgt attgatttt 
caggacact gcggctata tttaaacgc gttcaaggg 
ccgaggctc ggggtctcc gctcgacct ccattatgc 
tttatcgtg gttttgcgc acaattttt accaattat 
tgtgtgcca aaccatggg tttatctca tctactctt 
tttgtttta ccacatttg tctgcgtca tcctcagtt 
tgtaattaa atactattg tactttttt cattgttac 
aatgtagaa tagtcattt tttgtaact gtgatactt 
aaataataa gactctgga tgtgataat tgataacta 
ctttaacga gttcttcta ttttataac aacgatgat 
ttttggttc gacgttgca aataaatgt attattaaa 
tttatattt tagtcatta ttcatattt agatatagt 
atttatttt ccccatcct gtcccattt ctttgttgg 
tttcgaaaa gagcacaag atttttatt taaccacga 
aataagaga ggtaggtag cgacgacca aggctgggt 
tactagtaa tttttacaa gtcacgaaa tggcaccac 
tttcgatta acatttaac cctaagtta atactcttt 
ggaaaacac attttccat acgatttaa cttgaaaag 
ttaaagtta accataagc ggtcacgcc acttaaaaa 
gttcaatca atgctattt gaacaatgg tagagaagg 
taacgtaat ggtaaaata aagtagcac caccagggg 
cggatccag aattttcat cagggggtg aaatgtgag 
cctggataa tccaccata aaacgtaaa tttttttga 
atgaaagaa ttactgccc tttcagaaa tctgaaata 
ttttcctca caatgcaat aagtttaaa tttttagag 
gttcagtta ttaaaatcg taactggtc caatttgtt 
ctacaaaac gtttctcaa ttattaatt attgccaag 
aaataaatt tgtagattt taggtccaa gtcgttatc 
agcataata gatttgtcc aacaaacct aaattattc 
cgccaacat aataaccct tgataaaca taatcagct 
ttaatttcc accgaccat taaacattt ttgatttta
    • >TC014541 RC
      actcaaaag aaaatttgg taacttggt tttttactg 
atgtgacta aatttacaa aattgaatt atatattta 
agtgatttc aactagttt cgaaaaggc tgccgtgag 
attataaga aacaagtag aagtgttgt taaatgaaa 
ccaaagtgt agttggtga agcgtattg ggggttttg 
gatccggcc ctggcgcac cgccgctct cactcaatg 
tggtcagtt ggcagaggg taaaaattt gagttagag 
cggtggcga gtatattcg ttgagtaca tacagcctt 
attgataaa atttatgtt agttccagg atatgaaga 
ggattggta agtgtctcc aaagcttaa ttaatgtct 
gtggggtgg gaacaaaac atttcgaac aatttcaca 
caaaaaact ttttttgaa ggatgagac tgcagcaat 
ggtcacttg atgtgtgcc ggatgtttc acgcacatc 
ctcgccgat gcccgtttg cgtgacgaa acgtcaaca 
tgtcccact tgccgcgtg gaaatctgc aagacattg 
gcgacgaga aatttggct gttgaaaac gccgtctcg 
gaactaccc tccgaatgt cagttctgc aacaagcag 
tttccgagg aattccttg gaaaagcac gaagacgag 
gaatgcgag gacagaatt tcaggttgc aagtaccac 
aggatcggg tgtccgtgg agaggacca gtgcatgaa 
agggcgcaa catgaaaaa gaatgcgga catccgcat 
aagtctggg gcggaagtt atggaggct ttggaaata 
ctcgatcag cagctgatt gaagaaaga aaactatac 
aagacgata tttgagttg ttgggttac gataaaatt 
acttttaac gatattcag ttgaagccg tatcgtacc 
gacgatttt gtccatcga ttattttat gaatcatcg 
cgattttcg gccttcaat aatcagtgg gtagtcaaa 
gctaaaatt agtgataat cagaaggac cccacacaa 
agctcagaa cgagatatg acttatcag ttaatcctg 
aaatcaaaa acatctacc cccttgagt ttgagttac 
atcgtctta cgaggtcct gtcggagat gtgaagata 
aatcctcga gtctacaat ttcgatttc acagaacaa 
gaaaccgaa actccctac gaacacttt cccctacca 
gacacagct gaatgtaat cgtttacta gcagcaaaa 
acaattaat ttcaggtta atcatgttt ttggcctca 
aaataagtt attttcgca tcgtcgcac ccaaaattc 
tagttcgta tttacttat tctgaccct cattttctt 
ttttttttt ttggctgaa tatactgga atgtctatt 
taaaagaag tttgtattt tgattaagt tagctgaag 
atctctata gagtttagg agtttttat atttagatc 
tttatttta tacatttta tatattttt ggattgtga 
gatgttttt cgttttaca ataacattt gttttgtta 
ccaacattt gtgtgttgc cattatttt tagtttaat 
ttttttttg gaaacgttt taataacat tgtgtcatt 
ttatttaac taatggctt ttgtcactg tcattctca 
gcattataa aggtagcat agaataaat taatttata 
tattcttaa ttgtaagaa aagggatat tggtagaaa 
ttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY