Status
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Gene information
General information
TC014533 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5972512..5982444
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014533
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05645

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014533 RA
      MIPLIAIAG ILAVSAAPA EFYESRYDH 
      LDVESILNN RRMVNYYAA CLLSKGPCP 
      PQGVDLKRV LPEALQTNC AKCTEKQRT 
      AAYRSIKRL KKEYPKIWE QLRAVWDPD 
      DVFIRKFET SFESGKPSG VISTNTSPP 
      SPILSNRFG ENEEADAAS NVISSTPLP 
      PTTSTTTRT TLTTKFTTK PSTKPTNKP 
      VVVTKPPQA PPFATVGAN LQATVTFMA 
      ALKNIVIQL CQHKLEVIN SILSGR
    • >TC014533 RB
      MIPLIAIAG ILAVSAAPA EFYESRYDH LDVESILNN 
      RRMVNYYAA CLLSKGPCP PQGVDLKRV LPEALQTNC 
      AKCTEKQRT AAYRSIKRL KKEYPKIWE QLRAVWDPD 
      DVFIRKFET SFESGKPSG VISTNTSPP SPILSNRFG 
      ENEEADAAS NVISSTPLP PTTSTTTRT TLTTKFTTK 
      PSTKPTNKP VVVTKPPQA PPFATVGAN LQATVSFGT 
      NLVGGIVRS LGTLGSRVV ESGTKLANM VISAAIRP
    CDS sequence
    • >TC014533 RA
      atgatccct ctaattgcc attgccgga atactcgca 
      gtgtcagca gctcctgct gaattctac gagagcaga 
      tacgaccac ctcgatgtc gaaagcatc ctcaacaac 
      cgaagaatg gtcaactac tacgccgct tgcctgctg 
      tctaaagga ccttgtccg ccacaaggg gtggacctc 
      aaaagggtg ctcccagag gctctccag accaactgc 
      gccaaatgc accgaaaag cagcggaca gcagcctac 
      cgctccatc aagcgcctg aaaaaagaa tatccgaag 
      atctgggag cagttgcgc gcagtttgg gaccccgat 
      gatgtcttc atccgtaaa tttgaaacc agttttgaa 
      tctggaaaa ccttctggt gttattagt accaacact 
      tctcctcca agtccaatt ctgtcaaat cgttttgga 
      gagaacgaa gaagcggac gctgctagc aacgtcatc 
      tcttcgaca ccccttccc cccaccact agcactact 
      actcgcact actctaacc actaaattt actactaaa 
      cctagtact aaacctact aacaaacct gttgttgta 
      acaaaaccg ccacaagcg ccaccgttt gcgaccgtt 
      ggggctaat ctgcaagcc acggtcaca ttcatggcg 
      gcactaaaa aacattgtt atccagttg tgccaacac 
      aaactggag gttattaac agcatacta tctgggaga 
      taa
    • >TC014533 RB
      atgatccct ctaattgcc attgccgga atactcgca 
      gtgtcagca gctcctgct gaattctac gagagcaga 
      tacgaccac ctcgatgtc gaaagcatc ctcaacaac 
      cgaagaatg gtcaactac tacgccgct tgcctgctg 
      tctaaagga ccttgtccg ccacaaggg gtggacctc 
      aaaagggtg ctcccagag gctctccag accaactgc 
      gccaaatgc accgaaaag cagcggaca gcagcctac 
      cgctccatc aagcgcctg aaaaaagaa tatccgaag 
      atctgggag cagttgcgc gcagtttgg gaccccgat 
      gatgtcttc atccgtaaa tttgaaacc agttttgaa 
      tctggaaaa ccttctggt gttattagt accaacact 
      tctcctcca agtccaatt ctgtcaaat cgttttgga 
      gagaacgaa gaagcggac gctgctagc aacgtcatc 
      tcttcgaca ccccttccc cccaccact agcactact 
      actcgcact actctaacc actaaattt actactaaa 
      cctagtact aaacctact aacaaacct gttgttgta 
      acaaaaccg ccacaagcg ccaccgttt gcgaccgtt 
      ggggctaat ctgcaagcc acggtgagt tttggtact 
      aacttagtt ggaggtatt gtgcgtagc ttaggtact 
      ctgggttcg agagtggtc gagtcgggt actaaactg 
      gccaatatg gttatttca gcagctata aggccttaa
    mRNA sequence
    • >TC014533 RA
      ttcgccgaa agaagtagc gacctcgct ctcctcaca 
      ctgtacaag tttcttgca ggagacgat ttggaatca 
      gtagtgtgc accgaacca ctcgagcaa ttccattag 
      aaagaccag tgtcggggt gtcatcatg atccctcta 
      attgccatt gccggaata ctcgcagtg tcagcagct 
      cctgctgaa ttctacgag agcagatac gaccacctc 
      gatgtcgaa agcatcctc aacaaccga agaatggtc 
      aactactac gccgcttgc ctgctgtct aaaggacct 
      tgtccgcca caaggggtg gacctcaaa agggtgctc 
      ccagaggct ctccagacc aactgcgcc aaatgcacc 
      gaaaagcag cggacagca gcctaccgc tccatcaag 
      cgcctgaaa aaagaatat ccgaagatc tgggagcag 
      ttgcgcgca gtttgggac cccgatgat gtcttcatc 
      cgtaaattt gaaaccagt tttgaatct ggaaaacct 
      tctggtgtt attagtacc aacacttct cctccaagt 
      ccaattctg tcaaatcgt tttggagag aacgaagaa 
      gcggacgct gctagcaac gtcatctct tcgacaccc 
      cttcccccc accactagc actactact cgcactact 
      ctaaccact aaatttact actaaacct agtactaaa 
      cctactaac aaacctgtt gttgtaaca aaaccgcca 
      caagcgcca ccgtttgcg accgttggg gctaatctg 
      caagccacg gtcacattc atggcggca ctaaaaaac 
      attgttatc cagttgtgc caacacaaa ctggaggtt 
      attaacagc atactatct gggagataa gacttctaa 
      gcttaacac tgaagagtg agaagaagg atgtccttt 
      gttgtctgt tattctgtc attgtctca ttagctagg 
      actattaga gtcatgcca tttcaaccg tcatctttc 
      agatagact gatttttta ataggggtc tgaattggc 
      tgtgtcaaa tgaagacgt tacttagtc aaggttttg 
      actccatgg ccgccattt aaatttaat tcgaaagtc 
      aaagattca gttatcagg ttatctagt cgtacccat 
      aattggctt attgcacaa aaatgcaaa ccaaggtct 
      aataacgca tccaaatcc taataaagc ttttgtgaa 
      actaacaaa atctaaatt gcccccaaa aattattta 
      tttatgaag cctgatact cagttttgc gaaaaaccg 
      atctaactc ttaagtcat gtgatattc tttaatgta 
      atgttaatt ttagcagta aattgtata gttattagt 
      gaaataaaa aacaaaata tgac
    • >TC014533 RB
      agtcaagta cgtagcgaa aataaaaat tatctgcct 
      gtaatggag acgatttgg aatcagtag tgtgcaccg 
      aaccactcg agcaattcc attagaaag accagtgtc 
      ggggtgtca tcatgatcc ctctaattg ccattgccg 
      gaatactcg cagtgtcag cagctcctg ctgaattct 
      acgagagca gatacgacc acctcgatg tcgaaagca 
      tcctcaaca accgaagaa tggtcaact actacgccg 
      cttgcctgc tgtctaaag gaccttgtc cgccacaag 
      gggtggacc tcaaaaggg tgctcccag aggctctcc 
      agaccaact gcgccaaat gcaccgaaa agcagcgga 
      cagcagcct accgctcca tcaagcgcc tgaaaaaag 
      aatatccga agatctggg agcagttgc gcgcagttt 
      gggaccccg atgatgtct tcatccgta aatttgaaa 
      ccagttttg aatctggaa aaccttctg gtgttatta 
      gtaccaaca cttctcctc caagtccaa ttctgtcaa 
      atcgttttg gagagaacg aagaagcgg acgctgcta 
      gcaacgtca tctcttcga caccccttc cccccacca 
      ctagcacta ctactcgca ctactctaa ccactaaat 
      ttactacta aacctagta ctaaaccta ctaacaaac 
      ctgttgttg taacaaaac cgccacaag cgccaccgt 
      ttgcgaccg ttggggcta atctgcaag ccacggtga 
      gttttggta ctaacttag ttggaggta ttgtgcgta 
      gcttaggta ctctgggtt cgagagtgg tcgagtcgg 
      gtactaaac tggccaata tggttattt cagcagcta 
      taaggcctt aaatgttta ctactttgt agatatagg 
      tttgtgttt gtgtgtcac taatccacc atcaccctt 
      ttaggtcac attcatggc ggcactaaa aaacattgt 
      tatccagtt gtgccaaca caaactgga ggttattaa 
      cagcatact atctgggag ataagactt ctaagctta 
      acactgaag agtgagaag aaggatgtc ctttgttgt 
      ctgttattc tgtcattgt ctcattagc taggactat 
      tagagtcat gccatttca accgtcatc tttcagata 
      gactgattt tttaatagg ggtctgaat tggctgtgt 
      caaatgaag acgttactt agtcaaggt tttgactcc 
      atggccgcc atttaaatt taattcgaa agtcaaaga 
      ttcagttat caggttatc tagtcgtac ccataattg 
      gcttattgc acaaaaatg caaaccaag gtctaataa 
      cgcatccaa atcctaata aagcttttg tgaaactaa 
      caaaatcta aattgcccc caaaaatta tttatttat 
      gaagcctga tactcagtt ttgcgaaaa accgatcta 
      actcttaag tcatgtgat attctttaa tgtaatgtt 
      aattttagc agtaaattg tatagttat tagtgaaat 
      aaaaaacaa aatatgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY