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Gene information
General information
TC014517 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6402723..6404572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014517
    iBeetle screen iB_05642

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014517 RA
      MSEKTETRP KQEKSGRSS VIDSFVSQY ERYYSLSVL 
      IAVVSVVTI LIIAADECR TCFNRLEEV TQVTTSDGY 
      KTYWVYGKN VESGYLKHV FIILNRLGY KNDPNATDW 
      DLLWAHDYP FRKLYPQLN HLRPHQKVN HFPGCGYIT 
      NKVDLAVSG LKYIPPAFK LPQDKDNFL KYATSHPNV 
      TFVQKNNDH RNIKVKPVN EIDLNSEGT FVQEFVDKP 
      LLVSGHKFD IGVYTIITS IDPLRVYIY NGDALLRFC 
      PVKYYPFDP KNLDKYVVG DDYLPIWEV PALDYYYNE 
      LGFGMRESL NAFLRAKGK NPQTIWDQI EDAIRILIL 
      NKEPKILDV VAKFKSKRN FFELVRVDF VVDEDLNVF 
      LLEANMSPN LSSAHFPPN QLLYEQVLY NVMGLLGLG 
      ERIRKDSLA VRSHSEEEM MVSDKNLAT YPKECNSSL 
      CKSGCVSPV CQLCRPCLS ADTLEYLKE AYKEHLNRG 
      DCKRIFPPS QLKPEAEVK WEEYSAENQ LQYRWFLGK 
      CQLDATWC
    CDS sequence
    • >TC014517 RA
      atgtccgaa aagacagaa acacgaccc aaacaggaa 
      aaaagtggt cgtagcagt gtcatagac agtttcgtg 
      tcgcaatac gaacgatat tacagtctt tcagtatta 
      atagcagta gtttccgta gttacgatt ttaataata 
      gcggcggac gaatgccgg acgtgcttt aatcgctta 
      gaagaggtc actcaagtt acaacttcc gacggatat 
      aaaacttat tgggtttac ggcaaaaat gtggagagc 
      ggatacttg aagcacgtg tttataata ttaaatagg 
      ctcggctat aaaaacgac ccgaatgcc actgactgg 
      gacctatta tgggcccat gactaccca ttccgcaaa 
      ctttatcct caactaaac cacctgaga ccgcaccaa 
      aaagtcaat cattttcca gggtgtggt tacataaca 
      aataaggtt gatttagcc gtcagtgga cttaagtac 
      attccaccg gccttcaag ttaccacaa gacaaagac 
      aacttcctt aaatacgcc accagtcac cctaacgtt 
      actttcgta caaaaaaac aacgaccac cggaacatt 
      aaagtaaaa ccggttaat gaaattgat ttgaattca 
      gaggggact tttgtgcaa gaattcgtc gacaagcct 
      ttactagtc agtggccat aaattcgat atcggagtt 
      tacacaatt atcacctcg attgatccc ttgcgcgtt 
      tatatttac aacggagac gcgcttttg aggttttgt 
      ccggttaaa tattacccg tttgacccc aaaaatcta 
      gacaagtat gtggtcggc gacgattac ctcccaatt 
      tgggaagtc cctgctctc gattattat tacaacgag 
      ttgggattc gggatgagg gaaagtttg aacgctttt 
      cttcgggcc aaggggaaa aacccgcaa acaatttgg 
      gaccaaatc gaggacgcc atcaggatt ttaatccta 
      aataaagaa cccaaaatt ttggacgtc gtggccaaa 
      ttcaagtcc aaacgcaac tttttcgaa ctggtccga 
      gtcgacttt gtggtcgat gaagatctg aacgtgttt 
      ttactggag gcgaatatg agtcctaat ttgtcgtcg 
      gcacacttt ccccccaat cagttgctc tatgaacag 
      gtcttgtac aacgttatg gggctgtta ggactcgga 
      gagcgcatt cggaaagac tctcttgcc gttaggtca 
      cattcggaa gaggaaatg atggtctca gacaagaac 
      cttgccacg tatcccaaa gaatgtaac agctctctt 
      tgtaaaagt ggctgcgtt tcgccagtt tgtcaattg 
      tgtaggcct tgcctctct gctgatact ctcgaatat 
      ttgaaagaa gcgtacaag gagcatctg aatagaggg 
      gactgcaaa aggatattc cctccaagt caattaaaa 
      ccagaggcg gaagtgaag tgggaggag tattccgcc 
      gagaaccag cttcaatac agatggttt ctgggcaag 
      tgtcagttg gacgccacg tggtgctag
    mRNA sequence
    • >TC014517 RA
      taccgttac gtccacagt tccagttaa aatgtccga 
      aaagacaga aacacgacc caaacagga aaaaagtgg 
      tcgtagcag tgtcataga cagtttcgt gtcgcaata 
      cgaacgata ttacagtct ttcagtatt aatagcagt 
      agtttccgt agttacgat tttaataat agcggcgga 
      cgaatgccg gacgtgctt taatcgctt agaagaggt 
      cactcaagt tacaacttc cgacggata taaaactta 
      ttgggttta cggcaaaaa tgtggagag cggatactt 
      gaagcacgt gtttataat attaaatag gctcggcta 
      taaaaacga cccgaatgc cactgactg ggacctatt 
      atgggccca tgactaccc attccgcaa actttatcc 
      tcaactaaa ccacctgag accgcacca aaaagtcaa 
      tcattttcc agggtgtgg ttacataac aaataaggt 
      tgatttagc cgtcagtgg acttaagta cattccacc 
      ggccttcaa gttaccaca agacaaaga caacttcct 
      taaatacgc caccagtca ccctaacgt tactttcgt 
      acaaaaaaa caacgacca ccggaacat taaagtaaa 
      accggttaa tgaaattga tttgaattc agaggggac 
      ttttgtgca agaattcgt cgacaagcc tttactagt 
      cagtggcca taaattcga tatcggagt ttacacaat 
      tatcacctc gattgatcc cttgcgcgt ttatattta 
      caacggaga cgcgctttt gaggttttg tccggttaa 
      atattaccc gtttgaccc caaaaatct agacaagta 
      tgtggtcgg cgacgatta cctcccaat ttgggaagt 
      ccctgctct cgattatta ttacaacga gttgggatt 
      cgggatgag ggaaagttt gaacgcttt tcttcgggc 
      caaggggaa aaacccgca aacaatttg ggaccaaat 
      cgaggacgc catcaggat tttaatcct aaataaaga 
      acccaaaat tttggacgt cgtggccaa attcaagtc 
      caaacgcaa ctttttcga actggtccg agtcgactt 
      tgtggtcga tgaagatct gaacgtgtt tttactgga 
      ggcgaatat gagtcctaa tttgtcgtc ggcacactt 
      tccccccaa tcagttgct ctatgaaca ggtcttgta 
      caacgttat ggggctgtt aggactcgg agagcgcat 
      tcggaaaga ctctcttgc cgttaggtc acattcgga 
      agaggaaat gatggtctc agacaagaa ccttgccac 
      gtatcccaa agaatgtaa cagctctct ttgtaaaag 
      tggctgcgt ttcgccagt ttgtcaatt gtgtaggcc 
      ttgcctctc tgctgatac tctcgaata tttgaaaga 
      agcgtacaa ggagcatct gaatagagg ggactgcaa 
      aaggatatt ccctccaag tcaattaaa accagaggc 
      ggaagtgaa gtgggagga gtattccgc cgagaacca 
      gcttcaata cagatggtt tctgggcaa gtgtcagtt 
      ggacgccac gtggtgcta gcattaatt tagaaaata 
      aatacaaat tgaagagct gttaatttc ttgtaatta 
      attgttgga ataaattgt ttcctgttt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY