Status
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Gene information
General information
TC031150
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014501,TC014502
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11260227..11276720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031150
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    iBeetle screen iB_05641

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031150 RA
      MKLVIKVFN FVIVTFWVV SCQKTDDES RPYEFGFTI 
      DGEQHRHEK KDVNGIIQG EFGFITADG IYHVTVYAT 
      DENGNFKIL SMRNVRISA PLDGSPLPP GAKIESSPG 
      LGNQNPQPG TQVQKQPQP VQTTSRPKT TYVFTTQPT 
      IKPACGGCG YVTTPKPRP GERFPFQTP AFQKPSQDL 
      NAPNPGLNS PNPGLNSPN PGLNSPNSG SNYPNPGAN 
      LPNPSPNPL INAPIPGPV SPGFNEVER PRRPLSQVT 
      VSDGAIHVP GNPDIPIRD KFPGMVDGL PDGISEKDI 
      TDLLYKFNY TVGFHGHYE KGLKNGAKV GGYFVTGRD 
      GISRIVTYV ADENGFRPK VKFVRLDLN SDEVPKEGT 
      EKTFGLKNF EFVWHAHIS NSGAPDYSR AGQCDSLVS 
      ATVAVQHEC WVNGIMVHY GAVLMVILA VAPIESSSD 
      WNNPASAQY HIQTDEGPE RYFRYQTTS GQYRKEKRL 
      DDGTVVGTY AWIDADGFL RQRDYIADN AGYRILKTK 
      NLYVGRDTP IAQAIKSAK KVPPSAGTL VKGTTVAPL 
      NVEINQNSI SSTPRPTYL PSSTPAPFT INPWYYTSA 
      PKSFENGPT YPIDSRGQT YTGNNLGNG YDPQYDYYD 
      GVSVTNDGF RYYIPRAYH EEQNQGEKK AGSFGYIDP 
      FGIRRVIYY NAGPGTGFQ HRKNNRYVG FDATPYDPR 
      PY
    CDS sequence
    • >TC031150 RA
      atgaagctg gtcattaag gtttttaac tttgtaatt 
      gtgaccttt tgggtcgtc tcgtgtcaa aaaaccgat 
      gatgaaagc aggccttac gagttcggg tttaccatc 
      gacggagaa cagcacaga catgagaaa aaagatgtt 
      aatggaatc atccaaggc gaattcggc tttatcaca 
      gccgacggt atttatcac gtaaccgtg tacgccacc 
      gacgaaaat ggcaatttc aaaattttg agcatgaga 
      aacgtgcgg attagtgcc ccacttgat gggtccccg 
      ctacccccg ggggccaaa atcgagagc agtccgggc 
      ctgggcaac caaaaccca caacctgga acacaagtc 
      cagaaacaa ccacaacct gtccagacc acgtccagg 
      cccaagaca acctacgtc ttcaccact caacccaca 
      atcaagccg gcgtgtggt ggctgcggt tacgtcacg 
      acgcctaaa cctagacct ggggaacgg ttcccgttc 
      cagacgccc gccttccag aagcctagc caagatttg 
      aacgcaccc aacccaggt ttaaattcc ccaaaccca 
      ggtctaaat tcccccaac ccaggttta aattccccc 
      aattcgggc tcgaattat cccaatcct ggtgcgaat 
      ttgccaaat ccgagccca aatccgttg ataaatgcc 
      ccaattccg ggtccggtt tcgcccgga tttaacgaa 
      gtggagcgt ccccggagg ccgctttcg caggtgacg 
      gtgtcggac ggtgccatt cacgtgccc ggaaatccg 
      gatattccg atccgggat aagtttccg gggatggtt 
      gatggtttg ccggatggc atttcagag aaagatatc 
      accgatttg ttgtataag tttaattat accgttgga 
      tttcacggg cattacgag aaaggactg aagaatggt 
      gctaaggtt ggtgggtat tttgttacc ggaagagat 
      ggaattagc agaattgtc acgtatgtg gccgatgag 
      aatggattt agaccgaaa gtgaagttt gttagattg 
      gatttgaat tctgatgag gtgccgaaa gaaggaacg 
      gagaaaacg ttcggattg aagaatttc gagtttgtt 
      tggcacgcc cacatctct aactccgga gccccggat 
      tattcgcga gctggtcag tgcgatagt ttagttagt 
      gcaactgtg gcagtgcag cacgaatgc tgggtgaac 
      ggaatcatg gtgcattat ggtgctgtt ttaatggtg 
      atacttgcc gttgctccg atcgaatcg agcagtgac 
      tggaacaac ccagcaagt gcccagtac catatccaa 
      accgacgag ggccccgaa cgatacttc cgctaccag 
      acgaccagc ggccaatac cgcaaggaa aaacgactc 
      gacgatggt accgtcgtt ggtacctac gcctggatc 
      gacgccgat gggttcctc cgccagcgc gattacatc 
      gccgacaac gccggctac agaatcctc aaaacgaaa 
      aacctctac gttgggcga gacactccc attgcccaa 
      gccatcaag tcggcgaaa aaagtacca ccatccgct 
      ggtacttta gtcaagggc accaccgtg gccccactt 
      aacgtggaa atcaaccaa aactccatt tcctcaacc 
      cccagacca acgtatcta ccgtccagc accccagcg 
      ccctttacc atcaaccca tggtactac accagcgcg 
      cccaagagc ttcgaaaac gggcccacg taccccatc 
      gacagccgg gggcagacc tacacgggg aataatctg 
      ggcaatggg tacgacccc cagtacgac tactacgat 
      ggggtgtcg gtgaccaac gatgggttc cggtactac 
      atacccagg gcgtaccac gaggagcaa aatcagggg 
      gagaagaag gcggggagt tttgggtat atcgaccca 
      tttgggatc cgcagggtc atttattat aatgccggg 
      cctgggacg ggcttccag caccggaaa aataacaga 
      tatgtgggc ttcgatgcg acgccttac gatccaagg 
      ccttattaa
    mRNA sequence
    • >TC031150 RA
      tacgagttg taaaaattt aattacggt tgcagtggt 
      gattcgcaa tgaagctgg tcattaagg tttttaact 
      ttgtaattg tgacctttt gggtcgtct cgtgtcaaa 
      aaaccgatg atgaaagca ggccttacg agttcgggt 
      ttaccatcg acggagaac agcacagac atgagaaaa 
      aagatgtta atggaatca tccaaggcg aattcggct 
      ttatcacag ccgacggta tttatcacg taaccgtgt 
      acgccaccg acgaaaatg gcaatttca aaattttga 
      gcatgagaa acgtgcgga ttagtgccc cacttgatg 
      ggtccccgc tacccccgg gggccaaaa tcgagagca 
      gtccgggcc tgggcaacc aaaacccac aacctggaa 
      cacaagtcc agaaacaac cacaacctg tccagacca 
      cgtccaggc ccaagacaa cctacgtct tcaccactc 
      aacccacaa tcaagccgg cgtgtggtg gctgcggtt 
      acgtcacga cgcctaaac ctagacctg gggaacggt 
      tcccgttcc agacgcccg ccttccaga agcctagcc 
      aagatttga acgcaccca acccaggtt taaattccc 
      caaacccag gtctaaatt cccccaacc caggtttaa 
      attccccca attcgggct cgaattatc ccaatcctg 
      gtgcgaatt tgccaaatc cgagcccaa atccgttga 
      taaatgccc caattccgg gtccggttt cgcccggat 
      ttaacgaag tggagcgtc cccggaggc cgctttcgc 
      aggtgacgg tgtcggacg gtgccattc acgtgcccg 
      gaaatccgg atattccga tccgggata agtttccgg 
      ggatggttg atggtttgc cggatggca tttcagaga 
      aagatatca ccgatttgt tgtataagt ttaattata 
      ccgttggat ttcacgggc attacgaga aaggactga 
      agaatggtg ctaaggttg gtgggtatt ttgttaccg 
      gaagagatg gaattagca gaattgtca cgtatgtgg 
      ccgatgaga atggattta gaccgaaag tgaagtttg 
      ttagattgg atttgaatt ctgatgagg tgccgaaag 
      aaggaacgg agaaaacgt tcggattga agaatttcg 
      agtttgttt ggcacgccc acatctcta actccggag 
      ccccggatt attcgcgag ctggtcagt gcgatagtt 
      tagttagtg caactgtgg cagtgcagc acgaatgct 
      gggtgaacg gaatcatgg tgcattatg gtgctgttt 
      taatggtga tacttgccg ttgctccga tcgaatcga 
      gcagtgact ggaacaacc cagcaagtg cccagtacc 
      atatccaaa ccgacgagg gccccgaac gatacttcc 
      gctaccaga cgaccagcg gccaatacc gcaaggaaa 
      aacgactcg acgatggta ccgtcgttg gtacctacg 
      cctggatcg acgccgatg ggttcctcc gccagcgcg 
      attacatcg ccgacaacg ccggctaca gaatcctca 
      aaacgaaaa acctctacg ttgggcgag acactccca 
      ttgcccaag ccatcaagt cggcgaaaa aagtaccac 
      catccgctg gtactttag tcaagggca ccaccgtgg 
      ccccactta acgtggaaa tcaaccaaa actccattt 
      cctcaaccc ccagaccaa cgtatctac cgtccagca 
      ccccagcgc cctttacca tcaacccat ggtactaca 
      ccagcgcgc ccaagagct tcgaaaacg ggcccacgt 
      accccatcg acagccggg ggcagacct acacgggga 
      ataatctgg gcaatgggt acgaccccc agtacgact 
      actacgatg gggtgtcgg tgaccaacg atgggttcc 
      ggtactaca tacccaggg cgtaccacg aggagcaaa 
      atcaggggg agaagaagg cggggagtt ttgggtata 
      tcgacccat ttgggatcc gcagggtca tttattata 
      atgccgggc ctgggacgg gcttccagc accggaaaa 
      ataacagat atgtgggct tcgatgcga cgccttacg 
      atccaaggc cttattaaa tcattacaa acgccaaca 
      tgaacgatt taacatact aaacacgtg cttgtgaca 
      aatttttgc gaggtgatg atggatttc tccaatcac 
      tgattcata gttagggtt atttattga actaaaatt 
      gattgtgag tgagggggt cccgggaat cacagttta 
      taatcaaaa taatgtaaa taacgacaa taatcgatt 
      atggactct agttcgggg acctgattt tttcaatac 
      tctctcttg ttatgttag gtaatttat gataatgtt 
      ttgtaaata aatggataa aaattacat gcgttttat 
      ttgccggac gcattgttc ggttttatc gctcgttaa 
      gtaatgaaa tgaataaat aataagtga ttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY