Status
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Gene information
General information
TC033173
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014487
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11104313..11112521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033173
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05638

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033173 RA
      MNIPGVNLD PYSVKTETA LMHDAVHMF LKCITDLHA 
      TGKSVKPTK LSCENVDKW TPGFDIASF IKAYTHDTD 
      GLYSTTAPI SFDNLGRRT NFSIFVVEG NRDDVVAKW 
      NPSDPEVLQ FLKSEEDRN KELERKWSE GIVTVASII 
      GPPYLMVKE QKSETDLLE GNNRYEGFS MDLIALLAK 
      DLNIKFRFE VLKSGQRGA YDKTTKSWN GLIREILDR 
      RAELAICDL TITPDRREV VDFSTPFMR LGISILYRK 
      AEAKEADMY AFLDPFSLK LWMYSATLY LALTVVLFF 
      ISRISPQDW ENPHPCEQE PEELENIWD MKNCLWLTL 
      GSIMNQGCD ILPKGMAPR LAASMWWFF TIIVTNSYM 
      ANLAAFLTN ERSQSEINS AEDLAKQTK IKYGTLDGG 
      STQGFFRES NYSLYQRMW TAMEQAKPS VFEQSNDAG 
      VARVQNEKN RLYAFLMES STLEYQIQT KCDLKQVGN 
      WLDSKGYGI AMPLDYPHR SRINEALLR LQEQGEINR 
      LKDKWWKEE RKDPLCPKE SEDQDANKL ALQNVGGVF 
      IVLGVGVAL AYIVAVLEF LWNVRSVSV DEHISYMQA 
      LKVELLFAL DVRKTKKRA KPEVPESSS SSRSPSMAR 
      SFLQSAGSF LRLDKMNQM ETPGSSRHT SRPLE
    CDS sequence
    • >TC033173 RA
      atgaatatt cctggggtt aatttggat ccatactcc 
      gtcaagacc gagacggct ttgatgcac gacgccgtc 
      cacatgttt ttaaaatgt atcaccgac ctgcatgcc 
      acaggaaaa tcagtaaag ccgacgaaa ttgagctgt 
      gaaaatgtg gataaatgg acgcctggt ttcgacatt 
      gcttcattt attaaagcg tacactcac gacacggat 
      ggtttatac agcacaacg gcccctata agtttcgat 
      aatttaggc cgtaggacg aattttagc attttcgtg 
      gtcgagggc aatcgagac gacgttgtg gccaaatgg 
      aacccttca gaccccgaa gttttgcaa tttttgaaa 
      agcgaggag gatcggaat aaggaactg gagaggaaa 
      tggagcgaa ggaattgtc acggttgct tcaataatt 
      ggcccacct tacctcatg gtgaaggag caaaagtcg 
      gagactgat ctcctggaa ggcaacaac cgctacgag 
      ggtttttca atggacttg atagcctta ctggccaaa 
      gatctcaat atcaaattt aggtttgaa gtgctcaag 
      agtgggcaa cgaggggcc tatgacaag acgaccaaa 
      tcctggaac ggcctaatt cgcgagatt ttagaccga 
      agggccgaa ttggccatt tgtgacctg accatcacc 
      ccagatcgg agggaagtt gttgatttt agcacacct 
      ttcatgcgt ttggggatc agtattttg tacaggaaa 
      gcggaagct aaagaggcg gacatgtac gcctttttg 
      gaccctttc tcactgaaa ttgtggatg tattcagcc 
      accctgtac cttgcctta accgtcgta ttgtttttc 
      atttcgagg atttcgccc caagactgg gaaaacccg 
      cacccctgc gaacaagaa ccagaagag cttgaaaac 
      atctgggac atgaagaac tgtctttgg ctcactctt 
      ggctcaata atgaaccaa ggctgtgac attctccca 
      aagggcatg gcacctcga ctggccgcc tcaatgtgg 
      tggtttttc accataatc gtgaccaac tcctacatg 
      gcaaacctg gctgcattt ttgacaaac gagcggagc 
      caatcggag ataaacagt gccgaggac ttagccaaa 
      caaactaaa ataaaatac gggactctg gacggtggt 
      tccacgcaa ggttttttc agggagtca aactattcg 
      ctctatcag cgcatgtgg actgccatg gaacaagcc 
      aaaccgtcc gtttttgag caaagtaac gacgctgga 
      gttgctcga gtccagaac gagaaaaac cgactttat 
      gcctttctg atggaatcc tcaacgctt gagtaccaa 
      atccagacc aaatgcgac ttgaaacaa gttgggaac 
      tggctagac agcaaggga tatggaatc gcaatgccc 
      ttggattat ccgcaccgg agtcgcatt aacgaagct 
      ttgctcaga ttgcaagaa cagggcgag atcaacaga 
      ctgaaggac aagtggtgg aaggaggaa cgcaaagac 
      cccctctgt ccgaaagaa tctgaggac caagacgcc 
      aacaaactg gccctacag aacgtcggt ggtgtcttt 
      atagtcttg ggagtggga gtagctctg gcatacatt 
      gtggcagtt ttggaattt ctgtggaac gttcggagc 
      gtttcagtc gatgaacac atttcgtac atgcaagcc 
      ctcaaagtc gagcttttg ttcgcacta gacgtccgg 
      aagacgaaa aaacgggcc aaacccgaa gtgcccgaa 
      tcgtcctcg tcttcacgg tcgccaagt atggcccga 
      agtttccta caaagcgct ggatcgttc ctcagactc 
      gataaaatg aatcagatg gaaacgccc ggctctagc 
      agacacacc tccaggcct ttggagtaa
    mRNA sequence
    • >TC033173 RA
      ttagatttg ggtttagga tgcgcatac ggtggattt 
      caaactttt gaataccac ggcaaacat cacaacggt 
      tcggatttt tgatccggc aagtgataa ttttcagta 
      cgcgatcag taactggaa taattatgt gaagaaaat 
      gaatattcc tggggttaa tttggatcc atactccgt 
      caagaccga gacggcttt gatgcacga cgccgtcca 
      catgttttt aaaatgtat caccgacct gcatgccac 
      aggaaaatc agtaaagcc gacgaaatt gagctgtga 
      aaatgtgga taaatggac gcctggttt cgacattgc 
      ttcatttat taaagcgta cactcacga cacggatgg 
      tttatacag cacaacggc ccctataag tttcgataa 
      tttaggccg taggacgaa ttttagcat tttcgtggt 
      cgagggcaa tcgagacga cgttgtggc caaatggaa 
      cccttcaga ccccgaagt tttgcaatt tttgaaaag 
      cgaggagga tcggaataa ggaactgga gaggaaatg 
      gagcgaagg aattgtcac ggttgcttc aataattgg 
      cccacctta cctcatggt gaaggagca aaagtcgga 
      gactgatct cctggaagg caacaaccg ctacgaggg 
      tttttcaat ggacttgat agccttact ggccaaaga 
      tctcaatat caaatttag gtttgaagt gctcaagag 
      tgggcaacg aggggccta tgacaagac gaccaaatc 
      ctggaacgg cctaattcg cgagatttt agaccgaag 
      ggccgaatt ggccatttg tgacctgac catcacccc 
      agatcggag ggaagttgt tgattttag cacaccttt 
      catgcgttt ggggatcag tattttgta caggaaagc 
      ggaagctaa agaggcgga catgtacgc ctttttgga 
      ccctttctc actgaaatt gtggatgta ttcagccac 
      cctgtacct tgccttaac cgtcgtatt gtttttcat 
      ttcgaggat ttcgcccca agactggga aaacccgca 
      cccctgcga acaagaacc agaagagct tgaaaacat 
      ctgggacat gaagaactg tctttggct cactcttgg 
      ctcaataat gaaccaagg ctgtgacat tctcccaaa 
      gggcatggc acctcgact ggccgcctc aatgtggtg 
      gtttttcac cataatcgt gaccaactc ctacatggc 
      aaacctggc tgcattttt gacaaacga gcggagcca 
      atcggagat aaacagtgc cgaggactt agccaaaca 
      aactaaaat aaaatacgg gactctgga cggtggttc 
      cacgcaagg ttttttcag ggagtcaaa ctattcgct 
      ctatcagcg catgtggac tgccatgga acaagccaa 
      accgtccgt ttttgagca aagtaacga cgctggagt 
      tgctcgagt ccagaacga gaaaaaccg actttatgc 
      ctttctgat ggaatcctc aacgcttga gtaccaaat 
      ccagaccaa atgcgactt gaaacaagt tgggaactg 
      gctagacag caagggata tggaatcgc aatgccctt 
      ggattatcc gcaccggag tcgcattaa cgaagcttt 
      gctcagatt gcaagaaca gggcgagat caacagact 
      gaaggacaa gtggtggaa ggaggaacg caaagaccc 
      cctctgtcc gaaagaatc tgaggacca agacgccaa 
      caaactggc cctacagaa cgtcggtgg tgtctttat 
      agtcttggg agtgggagt agctctggc atacattgt 
      ggcagtttt ggaatttct gtggaacgt tcggagcgt 
      ttcagtcga tgaacacat ttcgtacat gcaagccct 
      caaagtcga gcttttgtt cgcactaga cgtccggaa 
      gacgaaaaa acgggccaa acccgaagt gcccgaatc 
      gtcctcgtc ttcacggtc gccaagtat ggcccgaag 
      tttcctaca aagcgctgg atcgttcct cagactcga 
      taaaatgaa tcagatgga aacgcccgg ctctagcag 
      acacacctc caggccttt ggagtaact tagttattt 
      ttgtgcagt ggcggaaat aaaaatagg ttttaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY