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Gene information
General information
TC014463 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10665429..10667513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014463
    iBeetle screen iB_05635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014463 RA
      MGVGATEGG LRRRMPLRR VGGGVATGA SGLGGRAMR 
YYRLWIYTC NTALLAGAL AFCGAAART VLFDFRRSL 
VPSLTLYQP SFLYAYLAL ATQGGALQL LGCVAALRL 
SEKLLNAYW LLLLVLLFG DVVLGAFWA FKFERICQE 
LKPTLRIRF QQEYGSSSE LTAMWDRLQ EEDSCCGVS 
GPSEFNSSL RWEIPPSCC VEPPPANST KCSKTHTSG 
CDERLLAYL KATASILAI LGYCVLAFL KLCFLGILR 
YEIREMIQK IRILRTELE LTNANGAVP GAIRAESAE 
SERESLLVR PESAALLSS PPTQTKNPN GNNNYEMRE 
YRSHENI
    CDS sequence
    • >TC014463 RA
      atgggtgtc ggtgcgact gaggggggg cttcgtagg 
cgaatgccg ctgcgacgt gtgggtggt ggtgtggca 
acgggcgcc tctggactt ggcggcagg gccatgcgc 
tactaccgc ctctggatc tacacttgc aacactgca 
cttctggcc ggagcctta gcattttgt ggggccgct 
gccagaacc gtccttttc gattttaga aggtcactg 
gtgccgtct ttgacctta taccagcct tcattcctt 
tacgcttat ctagcactc gccacgcaa ggaggggcc 
ctccaactt ttgggctgt gtggcggct ttacgtctt 
tccgaaaaa ctactcaac gcctattgg ttgcttttg 
cttgtcctg ctgttcggc gatgtggtc ctgggcgcc 
ttctgggcg ttcaagttc gaacgcatc tgccaggag 
ctgaagccc acgctgcgg atcaggttt cagcaggaa 
tacggaagc agcagcgag ctgacggcg atgtgggac 
cgcctccaa gaggaagac tcttgttgt ggggtgagc 
ggtcccagc gagttcaac agcagtctt cgatgggag 
attcctccc agttgttgt gtcgagcct cctccggcc 
aactcgaca aagtgctcc aagacccac acgtctggc 
tgtgacgaa agacttctg gcttacctc aaagccacg 
gcctcgatt cttgcaatt ttgggctat tgtgtgctc 
gccttcctc aagctgtgc ttcctcggg attttgcga 
tacgaaatc cgggagatg atccagaag atccggatc 
ctccggacc gagttggag ttgaccaac gcgaacggt 
gcggttccg ggcgcaatc cgggccgag agtgccgaa 
agcgaacgc gaatcgtta ctcgtgcga ccggaaagt 
gccgcttta ctgtcgtcg ccgccgacg caaacgaaa 
aatcccaac ggaaataat aattacgag atgagggag 
tatcgcagc cacgagaac atttga
    mRNA sequence
    • >TC014463 RA
      tggggggca atgggtgtc ggtgcgact gaggggggg 
cttcgtagg cgaatgccg ctgcgacgt gtgggtggt 
ggtgtggca acgggcgcc tctggactt ggcggcagg 
gccatgcgc tactaccgc ctctggatc tacacttgc 
aacactgca cttctggcc ggagcctta gcattttgt 
ggggccgct gccagaacc gtccttttc gattttaga 
aggtcactg gtgccgtct ttgacctta taccagcct 
tcattcctt tacgcttat ctagcactc gccacgcaa 
ggaggggcc ctccaactt ttgggctgt gtggcggct 
ttacgtctt tccgaaaaa ctactcaac gcctattgg 
ttgcttttg cttgtcctg ctgttcggc gatgtggtc 
ctgggcgcc ttctgggcg ttcaagttc gaacgcatc 
tgccaggag ctgaagccc acgctgcgg atcaggttt 
cagcaggaa tacggaagc agcagcgag ctgacggcg 
atgtgggac cgcctccaa gaggaagac tcttgttgt 
ggggtgagc ggtcccagc gagttcaac agcagtctt 
cgatgggag attcctccc agttgttgt gtcgagcct 
cctccggcc aactcgaca aagtgctcc aagacccac 
acgtctggc tgtgacgaa agacttctg gcttacctc 
aaagccacg gcctcgatt cttgcaatt ttgggctat 
tgtgtgctc gccttcctc aagctgtgc ttcctcggg 
attttgcga tacgaaatc cgggagatg atccagaag 
atccggatc ctccggacc gagttggag ttgaccaac 
gcgaacggt gcggttccg ggcgcaatc cgggccgag 
agtgccgaa agcgaacgc gaatcgtta ctcgtgcga 
ccggaaagt gccgcttta ctgtcgtcg ccgccgacg 
caaacgaaa aatcccaac ggaaataat aattacgag 
atgagggag tatcgcagc cacgagaac atttgacta 
aagtatcgc ctggagggc ttgttcaat cgcggtcgc 
tggggccgc gtgaagaaa ggccctctg gctacgccc 
ctcgaggag atcttcaag cgcaaaccc aggacgagc 
agagtgaag gacgacccc tgctgaaaa cgaactagt 
gctttgtct agctaatgc agtagagga aaatcacac 
gcaataata ttctcaagc cacaagttt ttttttcgc 
ttcttatca ttccgattg tacagtgta cataatata 
cagacttta gactactca aatagacag tgcaaaaac 
tgtgttaca aagactggt cccttttta ggaaggaga 
gacacaaga aggacgctc aactattgc gagtgtgtt 
tatatgagc ttattatta ttgttatag tgcctcgcc 
ttaagtaac ttagctaat aggccaatc aaactagaa 
tttaattaa tgaaaattt gtccagttt gattggctt 
ataaaagtc gaagtgcat tctggtcta ataaaagtt 
caagtttgc gtttccatt ttaaaaggg acgattttt 
tccctcgca tgccttcga tattatctg atctcgcag 
ttcagttca tgaatgaaa aaccggcca gtcgtaaat 
aacgagctg tggcggccg accaagttg ccaatagat 
ccttgacgg atcgtcctg aagccggag cgcaaaaac 
cgacaaagt ttaagatat ttccaagtc attttagat 
aaattccca atgacatct tttcacttc ttttgaagt 
cgagtcaat taagcaaaa agcgtaccg gctaattct 
aattaatag acctaataa tactttaat tgaaatacg 
cagtaattt atgacacac taaaaaaca aacacttat 
tacagctta ttagagcgt tctaagtaa ggtaaagta 
caaagccgt tttatatga tgcgtagtc tttagtaaa 
accttgtat cataattag actaacatt aaaccaaca 
ctaaaagta aaggaaaat cgcgtttaa taaaactaa 
aatgcctag agttagaag agcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY