Status
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Gene information
General information
TC033165
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014445,TC014447
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10479117..10509032
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033165
    iBeetle screen iB_05634

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Antennae are sometimes shortened.
    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Pretarsus are not slightly bent as usual.
    legs trochanter not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: In few cases trochanter are strongly shortened.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 6

    labial palp ventral position not fused - Number of animals showing the phenotype: 6
    wings not present - Number of animals showing the phenotype: 6

    legs shortened - Number of animals showing the phenotype: 6

    abdomen gin-traps size decreased - Number of animals showing the phenotype: 6

    abdomen shape irregular - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033165 RA
      MEIANAVGA TPGVNRTAR VMMEGVGAR IIRGPDWKW 
      GKQDGGEGH VGTVRNFES PDEVVVVWD NGTAANYRC 
      SGQYDLRIL DSAPTGVKH EGTMCDTCR QQPIFGIRW 
      KCAECGNYD LCSVCYHGD KHQLRHRFY RITTPGSER 
      AFLETRRKS KKIAVRGIF PGARVVRGV DWQWEDQDG 
      GNGRRGKVQ EIQDWSAAS PRSAAYVVW DNGSKNLYR 
      VGFEGMADL KVVNDAKGQ NVYRDHLPC LGEQGPGRS 
      GPPGLKIGD QVNVDLELE IVQSLQHGH GGWTDGMFE 
      CLNGVGTVV GIDEDHDIV VAYSSGNRW TFNPAVLTK 
      VATPTNSVV FNETPQQQF AVGDMVQIC SDMERVKML 
      QRGHGDWAD GMAATLGKI GHVQQIYND NDLKVEVCN 
      TTWTYNPLA VTKVASKDG TMHGTTSGE RLSALLKKL 
      FETHVSGDV NEELVKFAA NGDAQKCEE VLKQSNTSG 
      AGIGPDVNG VFAGHTALQ AASQNGHLE VITVLLRFH 
      ADVEIEDKD GDRAVHHAA FGDEPAVVQ LLAHAGADL 
      NARNKRRQT ALHIGVNKG HLGVVKMLL DLGCHPSLQ 
      DLEGDTPLH DAISKKRDD MLTLLLDHS ADITLTNNN 
      GFNALHHSA LRGNPR
    CDS sequence
    • >TC033165 RA
      atggagatc gccaacgcc gtgggggca accccagga 
      gtgaaccga accgctcgt gtgatgatg gaaggggtg 
      ggtgctcgg ataatccgc ggtcccgac tggaagtgg 
      ggcaaacag gacggtggc gagggccac gtaggcacc 
      gtccgtaac ttcgagtcg cctgacgaa gtggtcgta 
      gtgtgggac aacggcact gccgccaat taccgctgt 
      tccggtcag tacgaccta cgtatcctg gacagcgcc 
      cctacgggg gtgaaacac gagggcacg atgtgcgac 
      acgtgccgc caacaaccc atattcgga attcgctgg 
      aaatgcgcg gaatgtggc aactacgac ctgtgttcc 
      gtctgttat cacggcgac aagcaccaa ctccggcac 
      agattctac cgcattacg acccccgga agcgaacgc 
      gcgtttctc gagacgaga cgcaagagc aagaaaatc 
      gcggtacgc ggcattttt ccgggggct cgggtcgtg 
      cgaggggtg gactggcag tgggaggac caggatggg 
      ggcaacgga cgcagaggg aaggtccag gagatacag 
      gactggtct gccgcaagt cccagatca gcggcgtac 
      gtcgtgtgg gacaatggc tctaaaaat ttatacagg 
      gtggggttt gaagggatg gccgatttg aaagttgtg 
      aacgatgct aaggggcag aacgtgtac agggatcat 
      ctgccgtgt ctcggggag caagggccg gggcgttcg 
      gggcccccg ggactcaaa atcggggat caagtcaat 
      gttgatcta gaattggag attgtccag agtctgcaa 
      catggacat gggggctgg actgacggc atgttcgag 
      tgtctgaat ggggttggg actgttgtt gggattgac 
      gaggaccat gatattgtt gtcgcttat agtagtggg 
      aacaggtgg acttttaat cccgccgtt ttgacgaaa 
      gttgcgact ccgacaaat tcagtcgta tttaatgaa 
      accccacaa caacaattt gcagtcgga gatatggtc 
      cagatttgc tcggacatg gaacgcgtc aaaatgctt 
      caaaggggg catggagac tgggccgat ggaatggca 
      gctacactt ggaaaaatc ggccacgtg cagcaaatc 
      tacaacgac aacgacctc aaagttgag gtctgcaac 
      acgacctgg acctacaac cccctcgcc gtcaccaaa 
      gtggctagc aaagacggc acaatgcac ggcacgacc 
      agtggcgag cgtcttagc gcccttctc aagaaactt 
      ttcgagacc cacgtctca ggcgacgtc aatgaagaa 
      cttgttaaa tttgccgct aacggtgac gctcaaaaa 
      tgcgaggaa gttctcaaa cagagtaac acgtccggg 
      gccggcatt gggccggac gtgaatggg gttttcgcc 
      gggcatacg gccctgcaa gctgccagt cagaacggt 
      catttggaa gtaattacg gtgcttttg cgatttcat 
      gccgatgtg gagattgag gataaggac ggagacagg 
      gccgtacat catgcggct ttcggggat gaaccggcc 
      gtcgtgcag ttgttggcc catgccggg gctgatctg 
      aacgcgagg aataagagg agacagaca gcgttgcac 
      atcggggtc aataagggg catctaggg gtcgtgaag 
      atgttgctg gatttgggc tgtcatccg agtttgcag 
      gacttggag ggcgacaca cctctgcac gatgccatt 
      tcgaaaaag cgcgacgat atgctgacc ttgctcctg 
      gatcacagc gccgatatc acattgacc aacaataac 
      ggattcaac gccctccac cactccgct ctccgcgga 
      aatccaagg tag
    mRNA sequence
    • >TC033165 RA
      tcaaccgtg atgtcaaca ctagctcag agcattcaa 
      aactaaagt aaatatttt tcaatggag atcgccaac 
      gccgtgggg gcaacccca ggagtgaac cgaaccgct 
      cgtgtgatg atggaaggg gtgggtgct cggataatc 
      cgcggtccc gactggaag tggggcaaa caggacggt 
      ggcgagggc cacgtaggc accgtccgt aacttcgag 
      tcgcctgac gaagtggtc gtagtgtgg gacaacggc 
      actgccgcc aattaccgc tgttccggt cagtacgac 
      ctacgtatc ctggacagc gcccctacg ggggtgaaa 
      cacgagggc acgatgtgc gacacgtgc cgccaacaa 
      cccatattc ggaattcgc tggaaatgc gcggaatgt 
      ggcaactac gacctgtgt tccgtctgt tatcacggc 
      gacaagcac caactccgg cacagattc taccgcatt 
      acgaccccc ggaagcgaa cgcgcgttt ctcgagacg 
      agacgcaag agcaagaaa atcgcggta cgcggcatt 
      tttccgggg gctcgggtc gtgcgaggg gtggactgg 
      cagtgggag gaccaggat gggggcaac ggacgcaga 
      gggaaggtc caggagata caggactgg tctgccgca 
      agtcccaga tcagcggcg tacgtcgtg tgggacaat 
      ggctctaaa aatttatac agggtgggg tttgaaggg 
      atggccgat ttgaaagtt gtgaacgat gctaagggg 
      cagaacgtg tacagggat catctgccg tgtctcggg 
      gagcaaggg ccggggcgt tcggggccc ccgggactc 
      aaaatcggg gatcaagtc aatgttgat ctagaattg 
      gagattgtc cagagtctg caacatgga catgggggc 
      tggactgac ggcatgttc gagtgtctg aatggggtt 
      gggactgtt gttgggatt gacgaggac catgatatt 
      gttgtcgct tatagtagt gggaacagg tggactttt 
      aatcccgcc gttttgacg aaagttgcg actccgaca 
      aattcagtc gtatttaat gaaacccca caacaacaa 
      tttgcagtc ggagatatg gtccagatt tgctcggac 
      atggaacgc gtcaaaatg cttcaaagg gggcatgga 
      gactgggcc gatggaatg gcagctaca cttggaaaa 
      atcggccac gtgcagcaa atctacaac gacaacgac 
      ctcaaagtt gaggtctgc aacacgacc tggacctac 
      aaccccctc gccgtcacc aaagtggct agcaaagac 
      ggcacaatg cacggcacg accagtggc gagcgtctt 
      agcgccctt ctcaagaaa cttttcgag acccacgtc 
      tcaggcgac gtcaatgaa gaacttgtt aaatttgcc 
      gctaacggt gacgctcaa aaatgcgag gaagttctc 
      aaacagagt aacacgtcc ggggccggc attgggccg 
      gacgtgaat ggggttttc gccgggcat acggccctg 
      caagctgcc agtcagaac ggtcatttg gaagtaatt 
      acggtgctt ttgcgattt catgccgat gtggagatt 
      gaggataag gacggagac agggccgta catcatgcg 
      gctttcggg gatgaaccg gccgtcgtg cagttgttg 
      gcccatgcc ggggctgat ctgaacgcg aggaataag 
      aggagacag acagcgttg cacatcggg gtcaataag 
      gggcatcta ggggtcgtg aagatgttg ctggatttg 
      ggctgtcat ccgagtttg caggacttg gagggcgac 
      acacctctg cacgatgcc atttcgaaa aagcgcgac 
      gatatgctg accttgctc ctggatcac agcgccgat 
      atcacattg accaacaat aacggattc aacgccctc 
      caccactcc gctctccgc ggaaatcca aggtaggaa 
      tctcgagcg tattttcgc gtcttcctc gctccttat 
      atatacaat aattttcgt tgtggagcg cgaggacgt 
      gttgcttcc accggaaat tggcgccgt cggcactcg 
      catccgttt ccgtgcaca tcatcgtcg tcagattct 
      cgaaaagtc ggctccagt cggcactat ttacacact 
      gcccttcta aataaagca actccggga ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY