Status
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Gene information
General information
TC014443 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10472547..10475571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014443
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05633

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 3

    tropharium potentiallysize decreased - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014443 RA
      MVQAPDVSI GHRDFPQAL VLLNEMNVS AQHVASLVD 
      NMIQKVRKG ELSTDQGLS FLEMKYNML LSYLINLTF 
      VVLRKCSGE KIEGDPCID RLIEIRTVL EKIRPIDHK 
      LKYQIDKLV KTAVTGTNA NDPTTFRAN PDNLIDHSE 
      SEEELEKNT GEKGKSDIY VPPKLSAVH YMGDETAQE 
      RTKRLQERT KKHMLSSSI MQDLREEYL DAPIEVSQT 
      SRAQQILSK QQKEKEEYE EEYMTRLPV SKSEKHKRR 
      QLTTLGSLG DEITDFGPS PSSAGKRKR KRPIKSKGK 
      SFKRKRFH
    CDS sequence
    • >TC014443 RA
      atggttcag gcaccggat gttagtatc gggcaccgg 
      gattttccc caggcgtta gttttgcta aatgaaatg 
      aatgtgagt gcacaacac gtcgcctcg ttggttgat 
      aacatgatt cagaaggtg cggaaggga gaattatca 
      acagatcag ggtcttagc tttctagaa atgaagtac 
      aacatgttg ttgagttat ttgatcaac ttgactttc 
      gtggtcttg cggaaatgt tcaggggaa aaaattgaa 
      ggggacccg tgtattgat agattaatc gaaatcagg 
      actgtactt gagaaaatc aggcccatt gatcacaag 
      ctcaagtac cagattgat aaactcgtc aagactgcc 
      gttacggga accaacgcc aacgacccg accacgttt 
      agggcaaac cctgacaat ctcattgac cattcggaa 
      agtgaggaa gaattagaa aaaaacacg ggagaaaag 
      ggcaaaagc gacatctat gtacctcca aaactatcc 
      gcagttcac tatatgggt gacgaaacg gcacaagag 
      cgcactaaa aggttgcaa gaacgcacg aagaagcac 
      atgttgtcc tcttccata atgcaagac ttgcgggag 
      gagtatctg gatgcgcca atcgaggtg tctcagacc 
      agccgcgct cagcaaatc ctgtccaag cagcagaag 
      gagaaggag gagtacgag gaggagtac atgactcgc 
      ctccccgtc agcaaatca gagaagcac aaacgccgt 
      cagctgacc actctgggc agtctcggt gacgaaatc 
      accgacttt ggcccctcc cccagctcc gccggcaaa 
      aggaaaagg aaacgtccg atcaaatca aaagggaag 
      agtttcaaa cggaaacga ttccattga
    mRNA sequence
    • >TC014443 RA
      cgtgtcaaa tgtcagtcg ttcgaggtt agcaagtgt 
      cgttttgag ttacttccg ttctgtttt tagtttttg 
      caatttagg ctttagttt gtgcgattt ttaaaagtt 
      cgcgcataa tttttcatc gcgccgtgt acagtctgt 
      cacaagccg tacccccac ccttccgat atcgttgat 
      caaaaaagc tcccttcaa tataaaagt tgtgcacgt 
      gctgagctt atttttaca aaatttacg cgttgttat 
      gtattttta agttacgaa atcgacgga tcaaaatgg 
      ttcaggcac cggatgtta gtatcgggc accgggatt 
      ttccccagg cgttagttt tgctaaatg aaatgaatg 
      tgagtgcac aacacgtcg cctcgttgg ttgataaca 
      tgattcaga aggtgcgga agggagaat tatcaacag 
      atcagggtc ttagctttc tagaaatga agtacaaca 
      tgttgttga gttatttga tcaacttga ctttcgtgg 
      tcttgcgga aatgttcag gggaaaaaa ttgaagggg 
      acccgtgta ttgatagat taatcgaaa tcaggactg 
      tacttgaga aaatcaggc ccattgatc acaagctca 
      agtaccaga ttgataaac tcgtcaaga ctgccgtta 
      cgggaacca acgccaacg acccgacca cgtttaggg 
      caaaccctg acaatctca ttgaccatt cggaaagtg 
      aggaagaat tagaaaaaa acacgggag aaaagggca 
      aaagcgaca tctatgtac ctccaaaac tatccgcag 
      ttcactata tgggtgacg aaacggcac aagagcgca 
      ctaaaaggt tgcaagaac gcacgaaga agcacatgt 
      tgtcctctt ccataatgc aagacttgc gggaggagt 
      atctggatg cgccaatcg aggtgtctc agaccagcc 
      gcgctcagc aaatcctgt ccaagcagc agaaggaga 
      aggaggagt acgaggagg agtacatga ctcgcctcc 
      ccgtcagca aatcagaga agcacaaac gccgtcagc 
      tgaccactc tgggcagtc tcggtgacg aaatcaccg 
      actttggcc cctccccca gctccgccg gcaaaagga 
      aaaggaaac gtccgatca aatcaaaag ggaagagtt 
      tcaaacgga aacgattcc attgattag ttgggtcgt 
      cccgaccga tcattaaat caagagctt gaaacccca 
      gttgcctat ggtgacctt atgattttc aaacgtttt 
      ctcattgtc aaattcaaa attgtctag attagggtt 
      aatggttgt gttctttgt ttttcgtgt actaggatt 
      ttggcatgc actttggat gtttttcaa actgagttc 
      gttatagac gtcataata tagactgaa agtattttt 
      acatgatgt tatcaaact gttgcgcta cattctcac 
      gttacagac tgtaatgga acagtgaat taatttttg 
      tacttggac gtaggttct cgtaaaatt gtctcgggt 
      gggactgcg ttttgagta cgttcccga ttgccttgt 
      acaggttat atttatcgt tgttaacgt taaccacag 
      tttattttt cagactgga ttatcggtt gttgaaata 
      ttaagacaa ttgcagttt tcggttagt tttcacgtt 
      gttaaacat ttttttatt gttggcttt ttattttgt 
      taggtttgc tacattgcc acagttcag gtgggtttt 
      ttacgtctc gtcttgtgt tttagttgt agtgatatg 
      ttattaact agatataaa aatgtattc agtagctca 
      tgtcctttg gtgtactcg tatttcgta gaatattta 
      ggtatgcta ctgaaagtg acaaaaatt ctcagctca 
      aaaatttat taataatgt atcgtcata ggttataag 
      tttatggct ttaatcagt ttcattata cattcttta 
      tttgtagac atgaatttt tttaatata tattttttt 
      cttattgag agtttgtgt tttccccag tcatcggat 
      tgtaagaag ctgtgcgaa aacccttat atttctgca 
      attcgcaag tttctcgcc caaaactat caccgtaga 
      atttttcac tgctaagat cgggcggtt cgtggcgat 
      atttctgat tccgcataa acggaattt tcggaaaag 
      ccagctaca actcgtaat caagactag ttttttccc 
      cccataaaa ttacccttt gcataacat acggacgcg 
      gcacttgat aactgtgct gcatggtgg tcaaatatt 
      catcagcac atatcgtgc gacactgac tttccggat 
      attccgtca aacattcgg catttctgc aaaaaaccc 
      gtaaaaagt gccccaata tgaattcat caatactcg 
      ttgaaagtg taaaaactc ttcattctt ttggtccga 
      taaattctg tctttgatg aagacttgt tgtaacatg 
      tccccggtc gtggtcctg aacgagtta tcatcgttt 
      gtgcccaat ctgtgataa tgcgagaga aaataattg 
      actttccct tgacagtac gggtcgaca tcaatgggg 
      tgatgctct aggagttcc tgccaatac tcaaccttc 
      accccttcg gagtacccg ttcttggtg aatataatc 
      tggggcttg cggctgtgc gtgggcagc cgctcaggt 
      gcctcgagc cgaaatggg ggcattttt caaattatt 
      t
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