| General information | |||
| TC014438 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10428129..10455273 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014438 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05632 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg with embryonic tissue, no cuticle, no fluorescence -
Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014438 RB
MYHRHPKRQ THFRRTMET SIRPPLPPK GEMQSAPVP PPRRHRRAG SIGPSPCPS PTPMRHNQG VPTLARKDT FSFKDKMNS LKRMVGSRP LPPTPISPS SRVSASPPS FDRYNRGFE VPSPSPSLQ QRYRQHQLA MRGTTPNLS SAGSDFDKP PSRDSGYPD WDNEWGRFR SGSISGSET GSRLRKLSN TSCPPPRNL PHSASVFDL NNPMHHHHH HGFIPMQQA QSMAQLNCP GCYQGGWLE CGCDRTGSN LSLNMAPGP YPVNPMWMG TWHGPPPST MYPYPVPMG HMHHHSRPP SPTHSIKSR KSSMSKKSR RKYRESEDT DDDLEDRRS IFSHNDRSE RKSLGSRFP ERQKLPRET ASMPREISR KNSDRIERI SNVRSRRGS SSESDDEQS ESQKESEVL DESEEPEIK PILEVPTSN WECEHCTFV NDPGTKVCL VCCKTATIN VKLVKSDEK TEKPEPKLH TTRSSDDCS KDYSETESL LNKLGKLKT SEPKSPDPK KGKDASEAV SLESVSAVK AVGTSPEND TSVNKVTTG TSPPPQNMS TQTYEDVPP AGEIPKSPR GRPTSRNKR RDLRRSNSL HMGTRRGSE WSLHRSSSR HSLTTDSQS LPGSREQSP APYDYSEES MDRLRKPTM SRSYYSIMD LRKPELYRN EPPFYRNEY PPSHRNRSD SFDLPDSGF NSFKSQGME LVKLLREAE QYKYTADEV QAALCHCKD MNPIDWLRQ NWDATIASV QTLATQMGR EGPMNIVGT VSEEEARAA LRLHKGNLW PAVTECVEQ RQRKYAELA SRGEFSRED ILTVLTAHH GDLEAAFNE LSKTQLKPF LMRIWGPPV GTENEAGNE GAVLEKIGG EVVSDKELT KAVSDSSDK APPTTTDQE LKKTPTEVL ENIESQILQ NLQDMNNLS DTLDDAPPK PNKIYVEKS STVIQVFDH DYEVPEAER DEQKPESDF SDTESSDEG NKVEEFVDA VSDMPEPPR LKRKSVSTL NITLANKEE PGTSTGLPV AQMIQAEDT PTVNEVVLE STANIQLQS VPKNDQVGA SVNKKETEA SIVVPNGET KAQVETKNN LQEGSPTPV ATKEIKDNG ELSPETPSS QKETKTVNE NVQKTTKDD VSMVLVSQT KELQTDDLK NRDSSQNDT KDNQTNTSN QDHTKDSLD KGSINCTET KNDLNETPE GNVENKDPN EFSNKNSSI YEKTIENGN MQTSKNKEE TKTDLKEVA RRNETKGNL NVSKNKDVI TEQIINQSG VTKATNANE TVEHEVNKS QTNHSETKD NATEQITVE NDDANAPEG NEKTKNDQL APLESNNET KNDLNKSEN TVVAQKTIT SVDVKLSNE NEKTKHETN ENVKTSKDN EKTQNGLNS EKPSTSNGP STTEAKNHN EVVESKPPL TKKQKKSMK KSKRRAQKR AARRGSATS TTESSSGEK QSIETDNEV PEPVVKDYE PKIENSSSD NAEKPSTST ETQPKISTT IQIPQNKQS LLPVPPKRP SKIPISRQR SVSKSEPKS PDVQSKIPV KTGSHIPVR CLIEKAKSD TESEKRPVM QKRKSDEIA QEMQQSVQT VKEMNRQLN TVNRGSLSS FRNSSVEST TSSKQLSYT KSLDNDSDS SVSDSNVEE LLDPSTDED SYEDFEEYD GIEESDTED YNEFDKKKA KIANELDIN LAQISARVE ELTTSLGRK NEFDETCES EEEYVSEES EEEDEEEEE DDDDDEDNQ IDLNIEVKQ PSEIELMER QARRFLAEG QVESYQQAE LAVSLMTLK FSAEEALEA AKDCHTLDA AIAYLQQDC ELCAGKYPM NQIISMLKC THRCCQECA KNYFTVQVT DRTIMDCTC PFCKQPELT SSEISEDEV SDYFANLDI LLKGILDAT VHELFQRKL RDRTLMQDP NFKWCVKCS SGFIANPRQ RRLVCPDCK SVTCANCRR PWEKQHEGI SCEKFAEWK DANDPENQA SAVAKHLAE NGIDCPKCK FRYSLARGG CMHFTCTQC KHEFCYGCG KPFMMGAKC GVSQYCGKL GLHAHHPRN CLFYLRDKE PTELQKLLK EHKIPFDTD IPADKEENA AAAAKCFVP LQRETPNGL IDTVCNNEV SPGQAGLCR MHYLEYLCK LIRRHAIDS IDLLNADDL ETVVRRAAK RLPPNAIET RALNSSIAL FLSCLSMST AFLSASSPF RRSKSTAFL TISSISLVR NFLLIPEFV RIPRISFVK TAAVLRPNI FLRLQTYLK DYKPVYNRS LIEKAKSDT ESEKRPVMQ KRKLDEIAQ EMQQSVQTV KEMNRQLNT VNRGSLSSF RNSSVESTT SSKQLSYTK SLDNDSDSS VSDSNVEEL LDPSTDEDS YEDFEEYDG IEESDTEDY NEFDKKKAK IANELDINL AQISARVEE LTTSLGRKN EFDETCESE EEYVSEESE EEDEEEEED DDDDEDNQI DLNIEVKQP SEIELMERQ ARRFLAEGQ VESYQQAEL AVSLMTLKF SAEEALEAA KDCHTLDAA IAYLQQDCE LCAGKYPMN QVSVIFPAN FNQCSSGFI ANPRQRRLV CPDCKSVTC ANCRRPWEK QHEGISCEK FAEWKDAND PENQTSAVA KHLAENGID CPKCKFRYS LARGGCMHF TCTQCKHEF CYGCGKPFM MGAKCGVSQ YCGKLGLHA HHPRNCLFY LRDKEPTEL QKLLKEHKI PFDTDIPAD KEENAAAAA KCFVPLQRE TPNGLIDTV CNNEVSPGQ AGLCRMHYL EYLCKLIRR HAIDSIDLL NADDLETVV RRAAKRLPP NAYGTPREM LHYIEYLVG LIGRHKLDP ITILDLVEV SQELKRRGK DLPERTASC NDQEYRILC VKIVKEQIP LD
- >TC014438 RB
atgtaccac cgacacccc aaacgccag acccacttc cgacgcacc atggagact tcaatccgg cccccacta cccccaaaa ggagagatg caatccgcc cccgtgccg ccccctcgt cgccaccgc cgcgccggg tccatcgga ccgtcccca tgtcccagc cctaccccc atgagacac aaccagggg gttcccact ttggcgcgg aaagacact ttttcattc aaagacaag atgaatagt ctcaaacgg atggtgggg tcgaggcct ctacctccg acaccgatt tcaccctcg tcgcgtgtc tcagcgagc cccccgagt ttcgatagg tacaatcgc gggttcgaa gtaccaagt cccagcccc tccctgcag cagaggtac cgccaacat cagctggca atgaggggc accacccct aatctatca agtgccgga tctgatttt gataagccc ccgagccgc gatagcggg tacccagac tgggacaat gaatggggt cgcttccgg tcggggagc atttccggc tcggaaacc ggttcccgg ttacggaaa ttgagcaat acttcgtgc cccccgccc aggaatctc cctcacagc gcgtcggtg tttgacttg aataatcca atgcaccac caccaccat catgggttt attcccatg caacaagcc caaagtatg gcccagctg aactgtccg gggtgctac caagggggc tggttggaa tgtgggtgt gacaggacc ggcagtaac ttgagcctg aacatggcg ccagggccg tatccagtc aatcccatg tggatgggc acttggcat gggcctccg ccgagtacc atgtatcca tatcccgtc ccaatgggc cacatgcat caccactca agaccacca tcgcccacc cacagcatc aagtcccgg aagtcatcc atgagtaag aagagtcgg aggaagtac cgcgaaagt gaagacacc gatgatgat ttagaagac cgaagatcg attttcagc cacaatgat cgaagcgag aggaagtcg ctggggtca agattcccc gaaaggcag aaactgcca cgtgaaacc gcctccatg cctcgggaa atttcgaga aaaaattcg gaccgaatt gaacgaatc tcgaacgtg cgaagcaga cgtggttcg tccagtgag agcgacgac gagcaatcg gagagccag aaagagagt gaagtgctt gacgaatca gaagaacca gaaataaag ccaatactg gaagtaccg acctcgaat tgggagtgt gaacactgc accttcgtg aatgaccct ggcactaaa gtttgccta gtctgttgc aaaacggcc acaattaac gtcaaactc gtgaaaagc gacgagaaa accgagaaa cctgagcca aaattgcac acgactcga tcgagtgac gactgtagc aaagactac agcgagacc gaatctctt ttaaataaa ttgggcaaa ctaaaaacc tccgaaccg aaatccccc gacccgaag aaaggaaaa gacgcaagt gaagcggtg tcattggaa agtgttagt gcggttaaa gcggtcggt acttccccc gaaaatgac actagtgtt aataaagtg acaacgggc acatcgcct ccgccccaa aacatgtcg acacaaact tatgaggac gttcctcca gccggggaa atccccaag tcgccacga ggacgaccg acgagtcgc aacaaacga agagacttg cgaaggtcg aactcgctg cacatgggg acgagacgg gggtccgaa tggtcgctg caccgcagc tccagtcgg cactccctc accaccgac tcgcagagt ctccccggg agtcgggaa caaagccca gcgccgtat gactacagt gaggaaagt atggacagg ttgcgcaaa ccgacaatg tctcggtca tattacagc atcatggac ttgcgaaaa ccggaactt taccgaaac gaaccgccg ttttacagg aatgagtac cccccaagt caccgcaac cgctccgac tcgttcgac ctccccgac tcgggcttc aacagcttc aagtcgcag ggaatggag ctggtgaag ctcctccgc gaggctgag cagtacaag tacaccgct gacgaggtc caggcggcc ctgtgccac tgcaaggac atgaatccg atagattgg ctgcgccaa aactgggac gcgactatt gcgagcgtc cagactttg gccacgcag atggggcgc gagggcccc atgaatatc gtcgggacg gtgtctgag gaagaggcc agggccgct ttgagactc cacaagggg aatttgtgg ccagcggtg accgaatgt gtggagcaa aggcagaga aagtatgcc gaacttgcg tcacgtggg gagttcagt cgggaggat attctaaca gtgctaacg gcacatcac ggtgacttg gaggcggcg tttaacgag ctgagtaag actcaactc aagccgttt ctgatgcgg atttgggga ccgccggtt gggacggag aatgaggca ggtaatgag ggggcggtg ttggagaaa attgggggc gaagtagtg agcgataag gaactgaca aaagcggta agtgatagt agtgataaa gcgccccct acaaccacc gaccaggag ttaaaaaaa actccaact gaagttttg gaaaatatc gaatcgcag attttacaa aatttgcaa gacatgaac aatcttagt gacacttta gatgatgcg ccccctaaa cctaacaaa atttacgtg gagaaaagt agtaccgta atacaagtg tttgatcac gattatgaa gtcccggaa gccgagcga gacgaacaa aaaccagag tcggatttt agtgacact gaaagtagc gatgaaggt aacaaagtt gaggaattt gtggatgct gtgagtgat atgccagaa cctcctcgc ctcaaacga aaaagtgtc agtacttta aatatcact ttagccaat aaggaagaa ccgggaaca agtaccgga cttccagtt gcccaaatg attcaagct gaggatact ccgactgtg aatgaagta gtacttgaa agtactgca aatattcag ttacagagt gtcccaaaa aacgatcaa gtgggggcc tcagtgaat aaaaaagaa acagaggcc tccattgtg gttccaaat ggcgaaact aaagctcaa gtagaaaca aaaaataat ttacaagaa ggttccccg actccagtg gcaacaaaa gaaattaaa gataatggg gagctctct ccagaaaca ccctctagt caaaaagaa acaaaaacg gtgaatgaa aacgtacaa aaaacaaca aaagacgat gtaagtatg gttcttgta agtcaaaca aaagagctt caaactgat gatttaaaa aatcgtgat tcaagtcaa aatgatacc aaagacaat caaactaac acttcaaat caggatcac acaaaagat agtttagat aagggttca ataaattgt accgaaaca aaaaacgat ttaaatgag actccggaa ggcaatgta gaaaataaa gatccgaat gagttttcc aataagaat agttcaata tatgaaaaa acaattgaa aatggtaat atgcaaact tctaaaaat aaagaagag acgaaaact gatttaaaa gaggttgca agaagaaac gaaacaaaa ggaaatcta aatgtttct aaaaacaag gatgtaata acagaacag ataataaat cagagcggg gtaacaaaa gccacaaat gctaatgaa acagtagaa cacgaagta aataagagt caaacaaat catagtgaa acaaaagat aacgcgacg gaacagata accgtggaa aacgatgac gcaaacgct cctgaaggt aatgagaaa acaaaaaac gatcaattg gcgcccctt gaaagcaat aatgaaaca aaaaacgat ctgaataaa agcgaaaac actgtagta gcgcaaaaa acaattacg agtgttgat gtaaaacta tctaatgag aatgaaaaa acaaaacac gaaacaaat gaaaatgtt aaaacttcc aaagacaat gagaaaaca caaaacggc ctaaattca gaaaaacct tccacgagc aatggacca agtactacc gaagccaaa aatcataat gaggtcgta gaaagtaaa ccgccgttg acaaaaaag cagaaaaag tccatgaaa aaatcgaaa cgtcgcgcc cagaaacga gcagctcgg agaggctcg gcaacgagt actacggag tcaagcagt ggcgaaaaa cagtcaata gagacagac aacgaggtg cccgaaccc gtcgtcaaa gattatgaa ccaaaaatt gaaaattct tcctctgat aacgctgaa aaaccctcg acctcgacc gaaacccag ccaaaaatt tcaaccaca attcaaata ccccaaaat aaacaatct ctgcttccc gttcccccg aaaagacca tcaaaaatc ccgatttcg cgtcagaga tcagtttcc aaaagcgaa ccgaaatca cccgacgtg caaagtaaa atcccggtt aaaaccggg agtcatatt ccggttagg tgtttgatt gagaaagcg aaaagtgat acggaatcg gagaaacgt ccagttatg caaaaacgg aagtcggac gaaatcgca caagagatg caacaaagt gttcaaact gtcaaagag atgaaccga caattaaat actgtgaac agggggagt ctgagttcg tttcggaac agttccgtt gagtctacg acaagttcg aagcagctg tcttacaca aaatcacta gataatgat agcgattct tctgtttct gacagtaac gttgaagaa ttgctcgac ccgagcact gacgaagac agttacgag gattttgaa gagtatgat gggattgaa gagtccgac actgaggac tataacgag tttgataaa aagaaagcg aagattgcc aatgagctt gatattaat ttggcacaa attagtgca agggttgag gaattgacc acgagtttg ggtcgcaaa aatgagttt gatgagacg tgtgagtcc gaggaggag tatgtttct gaagaaagt gaggaggaa gatgaagaa gaggaagag gatgatgat gacgatgag gataatcaa attgatctt aatattgaa gtaaagcaa ccgagtgaa atcgagctg atggagagg caggcgagg cgcttcctg gccgagggc caagtcgag agctaccaa caagccgag cttgccgtt agcttaatg actcttaaa ttttccgct gaggaagct ctcgaagct gctaaagac tgccacacc ctagacgcc gctatcgca tatttgcaa caagactgc gaactgtgc gctggaaaa tatcccatg aatcagatc atatctatg ctaaaatgt acgcaccgc tgctgtcaa gaatgcgct aaaaattat tttaccgtg caagtgact gacaggacg ataatggac tgcacgtgc cctttctgc aaacaaccg gagttgaca agcagcgaa atatccgaa gacgaagtc tcggattat tttgccaat ttggatatt ctccttaaa ggcattcta gacgcaacc gtccatgag cttttccag aggaaattg cgcgaccgg actttaatg caagaccct aatttcaag tggtgcgtt aagtgttcg agcggattt atcgccaat cctcgccag agacgcctc gtttgtccc gactgcaaa agcgtcacg tgtgcaaac tgtcgccgg ccgtgggag aaacaacac gagggcatc tcttgcgag aaattcgca gaatggaaa gacgccaac gacccggag aaccaggcc tccgcggtt gcgaaacac ttagccgaa aacggcata gactgcccc aaatgtaaa tttcgatat tcactggcc cgagggggc tgcatgcat tttacttgc actcagtgc aaacacgag ttttgctac ggctgtggc aagcctttt atgatgggg gccaagtgt ggggtgagc cagtactgc gggaaattg gggctccat gcgcaccac ccccgcaac tgtctgttc tatttgcgc gataaggaa ccaacagag ctgcaaaag ttgctcaaa gagcacaaa atccccttc gataccgac atcccggcc gataaagag gaaaacgcc gcagccgca gctaagtgt ttcgtgccg ctgcagcgg gaaaccccc aacgggctc atcgacacg gtgtgcaac aacgaggtt tcccccggc caggccggt ctttgcagg atgcattat ttagagtac ttgtgtaag ttaataaga cgtcacgca atcgactca atcgacttg ttaaacgct gatgatctc gagactgtg gtcaggcgg gccgccaaa cgactgccg ccgaatgcg attgagacc cgtgcgctt aattcgtca attgctttg tttctttct tgtctttct atgtcaact gcttttttg agtgcttcg tcgcctttc cgtcgttct aaatcaact gctttttta actatttct tctatttcc ctcgtacgt aattttttg cttattcca gaattcgtt cgaatcccc cgaatctcg tttgttaaa actgcggct gtccttaga cccaacatc tttctgcgg ctacagacc tatttaaaa gattataaa ccagtttac aataggagt ttgattgag aaagcgaaa agtgatacg gaatcggag aaacgtcca gttatgcaa aaacggaag ttggacgaa atcgcacaa gagatgcaa caaagtgtt caaactgtc aaagagatg aaccgacaa ttaaatact gtgaacagg gggagtctg agttcgttt cggaacagt tccgttgag tctacgaca agttcgaag cagctgtct tacacaaaa tcactagat aatgatagc gattcttct gtttctgac agtaacgtt gaagaattg ctcgacccg agcactgac gaagacagt tacgaggat tttgaagag tatgatggg attgaagag tccgacact gaggactat aacgagttt gataaaaag aaagcgaag attgccaat gagcttgat attaatttg gcacagatt agtgcaagg gttgaggaa ttgaccacg agtttgggt cgcaaaaat gagtttgat gagacgtgt gagtccgag gaggagtat gtttctgaa gaaagtgag gaggaagat gaagaagag gaagaggat gatgatgac gatgaggat aatcaaatt gatcttaat attgaagta aagcaaccg agtgaaatc gagctgatg gagaggcag gcgaggcgc ttcctggcc gagggccaa gtcgagagc taccaacaa gccgagctt gccgttagc ttaatgact cttaaattt tccgctgag gaagctctc gaagctgct aaagactgc cacacccta gacgccgct atcgcatat ttgcaacaa gactgcgaa ctgtgcgct ggaaaatat cccatgaat caggtttct gtaattttc ccggctaac tttaatcag tgttcgagc ggatttatc gccaatcct cgccagaga cgcctcgtt tgtcccgac tgcaaaagc gtcacgtgt gcaaactgt cgccggccg tgggagaaa caacacgag ggcatctct tgcgagaaa ttcgcagaa tggaaagac gccaacgac ccggagaac cagacctcc gcggttgcg aaacactta gccgaaaac ggcatagac tgccccaaa tgtaaattt cgatattca ctggcccga gggggctgc atgcatttt acttgcact cagtgcaaa cacgagttt tgctacggc tgtggcaag ccttttatg atgggggcc aagtgtggg gtgagccag tactgcggg aaattgggg ctccatgcg caccacccc cgcaactgt ctgttctat ttgcgcgat aaggaacca acagagctg caaaagttg ctcaaagag cacaaaatc cccttcgat accgacatc ccggccgat aaagaggaa aacgccgca gccgcagct aagtgtttc gtgccgctg cagcgggaa acccccaac gggctcatc gacacggtg tgcaacaac gaggtttcc cccggccag gccggtctt tgcaggatg cattattta gagtacttg tgtaagtta ataagacgt cacgcaatc gactcaatc gacttgtta aacgctgat gatctcgag actgtggtc aggcgggcc gccaaacga ctgccgccg aatgcgtac ggtacgccc cgagaaatg ctgcattac atcgagtat ttagttggg ctcattggc agacacaaa ctggaccca atcacgatc ctggacctg gtggaagtc agtcaggag ttgaagaga cgcggcaaa gacttgccg gaaagaacg gctagttgt aacgaccag gagtacaga attttgtgt gtcaagatt gtgaaggag cagattcca ctggat
- >TC014438 RB
gtcaggggc gccaccccc gccaattcc aatcgagga gaagcccca ctcgtcgtt ttcaaaaac ccaagaacc cgattatga agttatcga atttggcca acagtactc aaatgcgcc cccaatacc catcaaaac agagcccaa aagggccga tggaaggca ctgccagct ctgtggtac ctccaaccc ttccatcaa atgcgacca ctgcaacca gatcttctg tatttcctg cgatgatat gtaccaccg acaccccaa acgccagac ccacttccg acgcaccat ggagacttc aatccggcc cccactacc cccaaaagg agagatgca atccgcccc cgtgccgcc ccctcgtcg ccaccgccg cgccgggtc catcggacc gtccccatg tcccagccc tacccccat gagacacaa ccagggggt tcccacttt ggcgcggaa agacacttt ttcattcaa agacaagat gaatagtct caaacggat ggtggggtc gaggcctct acctccgac accgatttc accctcgtc gcgtgtctc agcgagccc cccgagttt cgataggta caatcgcgg gttcgaagt accaagtcc cagcccctc cctgcagca gaggtaccg ccaacatca gctggcaat gaggggcac cacccctaa tctatcaag tgccggatc tgattttga taagccccc gagccgcga tagcgggta cccagactg ggacaatga atggggtcg cttccggtc ggggagcat ttccggctc ggaaaccgg ttcccggtt acggaaatt gagcaatac ttcgtgccc cccgcccag gaatctccc tcacagcgc gtcggtgtt tgacttgaa taatccaat gcaccacca ccaccatca tgggtttat tcccatgca acaagccca aagtatggc ccagctgaa ctgtccggg gtgctacca agggggctg gttggaatg tgggtgtga caggaccgg cagtaactt gagcctgaa catggcgcc agggccgta tccagtcaa tcccatgtg gatgggcac ttggcatgg gcctccgcc gagtaccat gtatccata tcccgtccc aatgggcca catgcatca ccactcaag accaccatc gcccaccca cagcatcaa gtcccggaa gtcatccat gagtaagaa gagtcggag gaagtaccg cgaaagtga agacaccga tgatgattt agaagaccg aagatcgat tttcagcca caatgatcg aagcgagag gaagtcgct ggggtcaag attccccga aaggcagaa actgccacg tgaaaccgc ctccatgcc tcgggaaat ttcgagaaa aaattcgga ccgaattga acgaatctc gaacgtgcg aagcagacg tggttcgtc cagtgagag cgacgacga gcaatcgga gagccagaa agagagtga agtgcttga cgaatcaga agaaccaga aataaagcc aatactgga agtaccgac ctcgaattg ggagtgtga acactgcac cttcgtgaa tgaccctgg cactaaagt ttgcctagt ctgttgcaa aacggccac aattaacgt caaactcgt gaaaagcga cgagaaaac cgagaaacc tgagccaaa attgcacac gactcgatc gagtgacga ctgtagcaa agactacag cgagaccga atctctttt aaataaatt gggcaaact aaaaacctc cgaaccgaa atcccccga cccgaagaa aggaaaaga cgcaagtga agcggtgtc attggaaag tgttagtgc ggttaaagc ggtcggtac ttcccccga aaatgacac tagtgttaa taaagtgac aacgggcac atcgcctcc gccccaaaa catgtcgac acaaactta tgaggacgt tcctccagc cggggaaat ccccaagtc gccacgagg acgaccgac gagtcgcaa caaacgaag agacttgcg aaggtcgaa ctcgctgca catggggac gagacgggg gtccgaatg gtcgctgca ccgcagctc cagtcggca ctccctcac caccgactc gcagagtct ccccgggag tcgggaaca aagcccagc gccgtatga ctacagtga ggaaagtat ggacaggtt gcgcaaacc gacaatgtc tcggtcata ttacagcat catggactt gcgaaaacc ggaacttta ccgaaacga accgccgtt ttacaggaa tgagtaccc cccaagtca ccgcaaccg ctccgactc gttcgacct ccccgactc gggcttcaa cagcttcaa gtcgcaggg aatggagct ggtgaagct cctccgcga ggctgagca gtacaagta caccgctga cgaggtcca ggcggccct gtgccactg caaggacat gaatccgat agattggct gcgccaaaa ctgggacgc gactattgc gagcgtcca gactttggc cacgcagat ggggcgcga gggccccat gaatatcgt cgggacggt gtctgagga agaggccag ggccgcttt gagactcca caaggggaa tttgtggcc agcggtgac cgaatgtgt ggagcaaag gcagagaaa gtatgccga acttgcgtc acgtgggga gttcagtcg ggaggatat tctaacagt gctaacggc acatcacgg tgacttgga ggcggcgtt taacgagct gagtaagac tcaactcaa gccgtttct gatgcggat ttggggacc gccggttgg gacggagaa tgaggcagg taatgaggg ggcggtgtt ggagaaaat tgggggcga agtagtgag cgataagga actgacaaa agcggtaag tgatagtag tgataaagc gccccctac aaccaccga ccaggagtt aaaaaaaac tccaactga agttttgga aaatatcga atcgcagat tttacaaaa tttgcaaga catgaacaa tcttagtga cactttaga tgatgcgcc ccctaaacc taacaaaat ttacgtgga gaaaagtag taccgtaat acaagtgtt tgatcacga ttatgaagt cccggaagc cgagcgaga cgaacaaaa accagagtc ggattttag tgacactga aagtagcga tgaaggtaa caaagttga ggaatttgt ggatgctgt gagtgatat gccagaacc tcctcgcct caaacgaaa aagtgtcag tactttaaa tatcacttt agccaataa ggaagaacc gggaacaag taccggact tccagttgc ccaaatgat tcaagctga ggatactcc gactgtgaa tgaagtagt acttgaaag tactgcaaa tattcagtt acagagtgt cccaaaaaa cgatcaagt gggggcctc agtgaataa aaaagaaac agaggcctc cattgtggt tccaaatgg cgaaactaa agctcaagt agaaacaaa aaataattt acaagaagg ttccccgac tccagtggc aacaaaaga aattaaaga taatgggga gctctctcc agaaacacc ctctagtca aaaagaaac aaaaacggt gaatgaaaa cgtacaaaa aacaacaaa agacgatgt aagtatggt tcttgtaag tcaaacaaa agagcttca aactgatga tttaaaaaa tcgtgattc aagtcaaaa tgataccaa agacaatca aactaacac ttcaaatca ggatcacac aaaagatag tttagataa gggttcaat aaattgtac cgaaacaaa aaacgattt aaatgagac tccggaagg caatgtaga aaataaaga tccgaatga gttttccaa taagaatag ttcaatata tgaaaaaac aattgaaaa tggtaatat gcaaacttc taaaaataa agaagagac gaaaactga tttaaaaga ggttgcaag aagaaacga aacaaaagg aaatctaaa tgtttctaa aaacaagga tgtaataac agaacagat aataaatca gagcggggt aacaaaagc cacaaatgc taatgaaac agtagaaca cgaagtaaa taagagtca aacaaatca tagtgaaac aaaagataa cgcgacgga acagataac cgtggaaaa cgatgacgc aaacgctcc tgaaggtaa tgagaaaac aaaaaacga tcaattggc gccccttga aagcaataa tgaaacaaa aaacgatct gaataaaag cgaaaacac tgtagtagc gcaaaaaac aattacgag tgttgatgt aaaactatc taatgagaa tgaaaaaac aaaacacga aacaaatga aaatgttaa aacttccaa agacaatga gaaaacaca aaacggcct aaattcaga aaaaccttc cacgagcaa tggaccaag tactaccga agccaaaaa tcataatga ggtcgtaga aagtaaacc gccgttgac aaaaaagca gaaaaagtc catgaaaaa atcgaaacg tcgcgccca gaaacgagc agctcggag aggctcggc aacgagtac tacggagtc aagcagtgg cgaaaaaca gtcaataga gacagacaa cgaggtgcc cgaacccgt cgtcaaaga ttatgaacc aaaaattga aaattcttc ctctgataa cgctgaaaa accctcgac ctcgaccga aacccagcc aaaaatttc aaccacaat tcaaatacc ccaaaataa acaatctct gcttcccgt tcccccgaa aagaccatc aaaaatccc gatttcgcg tcagagatc agtttccaa aagcgaacc gaaatcacc cgacgtgca aagtaaaat cccggttaa aaccgggag tcatattcc ggttaggtg tttgattga gaaagcgaa aagtgatac ggaatcgga gaaacgtcc agttatgca aaaacggaa gtcggacga aatcgcaca agagatgca acaaagtgt tcaaactgt caaagagat gaaccgaca attaaatac tgtgaacag ggggagtct gagttcgtt tcggaacag ttccgttga gtctacgac aagttcgaa gcagctgtc ttacacaaa atcactaga taatgatag cgattcttc tgtttctga cagtaacgt tgaagaatt gctcgaccc gagcactga cgaagacag ttacgagga ttttgaaga gtatgatgg gattgaaga gtccgacac tgaggacta taacgagtt tgataaaaa gaaagcgaa gattgccaa tgagcttga tattaattt ggcacaaat tagtgcaag ggttgagga attgaccac gagtttggg tcgcaaaaa tgagtttga tgagacgtg tgagtccga ggaggagta tgtttctga agaaagtga ggaggaaga tgaagaaga ggaagagga tgatgatga cgatgagga taatcaaat tgatcttaa tattgaagt aaagcaacc gagtgaaat cgagctgat ggagaggca ggcgaggcg cttcctggc cgagggcca agtcgagag ctaccaaca agccgagct tgccgttag cttaatgac tcttaaatt ttccgctga ggaagctct cgaagctgc taaagactg ccacaccct agacgccgc tatcgcata tttgcaaca agactgcga actgtgcgc tggaaaata tcccatgaa tcagatcat atctatgct aaaatgtac gcaccgctg ctgtcaaga atgcgctaa aaattattt taccgtgca agtgactga caggacgat aatggactg cacgtgccc tttctgcaa acaaccgga gttgacaag cagcgaaat atccgaaga cgaagtctc ggattattt tgccaattt ggatattct ccttaaagg cattctaga cgcaaccgt ccatgagct tttccagag gaaattgcg cgaccggac tttaatgca agaccctaa tttcaagtg gtgcgttaa gtgttcgag cggatttat cgccaatcc tcgccagag acgcctcgt ttgtcccga ctgcaaaag cgtcacgtg tgcaaactg tcgccggcc gtgggagaa acaacacga gggcatctc ttgcgagaa attcgcaga atggaaaga cgccaacga cccggagaa ccaggcctc cgcggttgc gaaacactt agccgaaaa cggcataga ctgccccaa atgtaaatt tcgatattc actggcccg agggggctg catgcattt tacttgcac tcagtgcaa acacgagtt ttgctacgg ctgtggcaa gccttttat gatgggggc caagtgtgg ggtgagcca gtactgcgg gaaattggg gctccatgc gcaccaccc ccgcaactg tctgttcta tttgcgcga taaggaacc aacagagct gcaaaagtt gctcaaaga gcacaaaat ccccttcga taccgacat cccggccga taaagagga aaacgccgc agccgcagc taagtgttt cgtgccgct gcagcggga aacccccaa cgggctcat cgacacggt gtgcaacaa cgaggtttc ccccggcca ggccggtct ttgcaggat gcattattt agagtactt gtgtaagtt aataagacg tcacgcaat cgactcaat cgacttgtt aaacgctga tgatctcga gactgtggt caggcgggc cgccaaacg actgccgcc gaatgcgat tgagacccg tgcgcttaa ttcgtcaat tgctttgtt tctttcttg tctttctat gtcaactgc ttttttgag tgcttcgtc gcctttccg tcgttctaa atcaactgc ttttttaac tatttcttc tatttccct cgtacgtaa ttttttgct tattccaga attcgttcg aatcccccg aatctcgtt tgttaaaac tgcggctgt ccttagacc caacatctt tctgcggct acagaccta tttaaaaga ttataaacc agtttacaa taggagttt gattgagaa agcgaaaag tgatacgga atcggagaa acgtccagt tatgcaaaa acggaagtt ggacgaaat cgcacaaga gatgcaaca aagtgttca aactgtcaa agagatgaa ccgacaatt aaatactgt gaacagggg gagtctgag ttcgtttcg gaacagttc cgttgagtc tacgacaag ttcgaagca gctgtctta cacaaaatc actagataa tgatagcga ttcttctgt ttctgacag taacgttga agaattgct cgacccgag cactgacga agacagtta cgaggattt tgaagagta tgatgggat tgaagagtc cgacactga ggactataa cgagtttga taaaaagaa agcgaagat tgccaatga gcttgatat taatttggc acagattag tgcaagggt tgaggaatt gaccacgag tttgggtcg caaaaatga gtttgatga gacgtgtga gtccgagga ggagtatgt ttctgaaga aagtgagga ggaagatga agaagagga agaggatga tgatgacga tgaggataa tcaaattga tcttaatat tgaagtaaa gcaaccgag tgaaatcga gctgatgga gaggcaggc gaggcgctt cctggccga gggccaagt cgagagcta ccaacaagc cgagcttgc cgttagctt aatgactct taaattttc cgctgagga agctctcga agctgctaa agactgcca caccctaga cgccgctat cgcatattt gcaacaaga ctgcgaact gtgcgctgg aaaatatcc catgaatca ggtttctgt aattttccc ggctaactt taatcagtg ttcgagcgg atttatcgc caatcctcg ccagagacg cctcgtttg tcccgactg caaaagcgt cacgtgtgc aaactgtcg ccggccgtg ggagaaaca acacgaggg catctcttg cgagaaatt cgcagaatg gaaagacgc caacgaccc ggagaacca gacctccgc ggttgcgaa acacttagc cgaaaacgg catagactg ccccaaatg taaatttcg atattcact ggcccgagg gggctgcat gcattttac ttgcactca gtgcaaaca cgagttttg ctacggctg tggcaagcc ttttatgat gggggccaa gtgtggggt gagccagta ctgcgggaa attggggct ccatgcgca ccacccccg caactgtct gttctattt gcgcgataa ggaaccaac agagctgca aaagttgct caaagagca caaaatccc cttcgatac cgacatccc ggccgataa agaggaaaa cgccgcagc cgcagctaa gtgtttcgt gccgctgca gcgggaaac ccccaacgg gctcatcga cacggtgtg caacaacga ggtttcccc cggccaggc cggtctttg caggatgca ttatttaga gtacttgtg taagttaat aagacgtca cgcaatcga ctcaatcga cttgttaaa cgctgatga tctcgagac tgtggtcag gcgggccgc caaacgact gccgccgaa tgcgtacgg tacgccccg agaaatgct gcattacat cgagtattt agttgggct cattggcag acacaaact ggacccaat cacgatcct ggacctggt ggaagtcag tcaggagtt gaagagacg cggcaaaga cttgccgga aagaacggc tagttgtaa cgaccagga gtacagaat tttgtgtgt caagattgt gaaggagca gattccact ggattgagc gaaattaac cccacgtaa aaatttcca atgacataa tatgcagtg ttattgttt tca
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