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Gene information
General information
TC014435 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10412377..10413610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014435
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014435 RA
      MALRGIRVL ELAGLAPAP FCGMVLSDF GASVLRIDK 
      VGANIDLDC LGNGKKSIA LNLKDPNGA KIVRKLAKT 
      SDVLIEPFR KGVMERLGL GPETLMKEN PRLIYARLT 
      GWGQTGLYS ARAGHDINY LALSGLLSL FGRKGQKPI 
      FPVNLAADF GGGGLMCAL GILLALHER HSSGLGQVI 
      DSSMVEGTA YLGSWLFRS QKLPIWGNE RGSNQLDSG 
      SHFYEVYET KDGKFVSVG ALEPQFYEK LLQGLGLSY 
      DEAPQFDDY EKLKVLFAR KFKEKTQEE WCRIFDKAD 
      ACVAPVLTL EEAPEHPHN KERGCFARM GDDIIPKPA 
      PVLSRTPGE ASGLKAAPN CGEHTWDVL KGLGYSDNN 
      INDLVAKGT VECFNKSKY
    CDS sequence
    • >TC014435 RA
      atggctttg cgaggcatc agggtgcta gagttggcc 
      ggattggcc ccagccccc ttttgtggt atggtcttg 
      tccgatttt ggggcttct gttttacgt atagacaag 
      gttggggct aacatagac cttgactgt ttgggtaat 
      ggcaaaaaa tcaatagcc ttgaacttg aaggaccca 
      aacggagcg aagattgtg agaaaattg gccaaaacc 
      agcgatgtc ctgattgag ccgtttaga aaaggagta 
      atggagagg ttggggtta ggtcctgag accctcatg 
      aaggaaaac ccgcgcttg atttatgcc cggttgacg 
      ggctggggc cagaccggg ttgtatagc gcgagggcg 
      ggtcacgat ataaactac ttggcgtta tcagggcta 
      ttatcactt tttgggcga aagggccaa aaaccaatt 
      tttcctgtg aatctggcc gctgatttc ggaggcggg 
      gggctgatg tgcgctttg gggattctc ttagcgctt 
      cacgagcgt cattcgtcc ggtttgggg caagttata 
      gatagcagt atggtcgaa gggacggcc tacttaggg 
      agctggttg tttcgttct cagaaactc ccgatttgg 
      gggaacgaa cgtggaagt aatcagctg gactcgggt 
      agtcatttt tatgaggtt tatgagaca aaagacggg 
      aaatttgtc tcagtgggg gcactagaa ccacaattc 
      tacgaaaaa ttgttacaa ggtttgggt ttgagttac 
      gatgaagcg ccacaattt gacgattac gaaaagttg 
      aaagtttta ttcgcgcga aaatttaaa gagaagacg 
      caggaagag tggtgccgg atttttgac aaagctgat 
      gcttgtgta gccccagtt ttaacgctt gaggaggct 
      ccagagcac ccacataat aaagaaagg gggtgtttt 
      gcgaggatg ggggatgat ataatcccc aaacctgcc 
      ccggtttta agtagaacg cctggagaa gcgagtggg 
      ttgaaagcg gcccccaac tgtggggaa catacgtgg 
      gatgttttg aaagggtta gggtacagt gacaataat 
      atcaatgat ttagttgcc aaaggcacg gttgaatgt 
      tttaataag tccaaatat taa
    mRNA sequence
    • >TC014435 RA
      tgcgtatga aaacattcg tttctgatg gctttgcga 
      ggcatcagg gtgctagag ttggccgga ttggcccca 
      gcccccttt tgtggtatg gtcttgtcc gattttggg 
      gcttctgtt ttacgtata gacaaggtt ggggctaac 
      atagacctt gactgtttg ggtaatggc aaaaaatca 
      atagccttg aacttgaag gacccaaac ggagcgaag 
      attgtgaga aaattggcc aaaaccagc gatgtcctg 
      attgagccg tttagaaaa ggagtaatg gagaggttg 
      gggttaggt cctgagacc ctcatgaag gaaaacccg 
      cgcttgatt tatgcccgg ttgacgggc tggggccag 
      accgggttg tatagcgcg agggcgggt cacgatata 
      aactacttg gcgttatca gggctatta tcacttttt 
      gggcgaaag ggccaaaaa ccaattttt cctgtgaat 
      ctggccgct gatttcgga ggcgggggg ctgatgtgc 
      gctttgggg attctctta gcgcttcac gagcgtcat 
      tcgtccggt ttggggcaa gttatagat agcagtatg 
      gtcgaaggg acggcctac ttagggagc tggttgttt 
      cgttctcag aaactcccg atttggggg aacgaacgt 
      ggaagtaat cagctggac tcgggtagt catttttat 
      gaggtttat gagacaaaa gacgggaaa tttgtctca 
      gtgggggca ctagaacca caattctac gaaaaattg 
      ttacaaggt ttgggtttg agttacgat gaagcgcca 
      caatttgac gattacgaa aagttgaaa gttttattc 
      gcgcgaaaa tttaaagag aagacgcag gaagagtgg 
      tgccggatt tttgacaaa gctgatgct tgtgtagcc 
      ccagtttta acgcttgag gaggctcca gagcaccca 
      cataataaa gaaaggggg tgttttgcg aggatgggg 
      gatgatata atccccaaa cctgccccg gttttaagt 
      agaacgcct ggagaagcg agtgggttg aaagcggcc 
      cccaactgt ggggaacat acgtgggat gttttgaaa 
      gggttaggg tacagtgac aataatatc aatgattta 
      gttgccaaa ggcacggtt gaatgtttt aataagtcc 
      aaatattaa ttttttcaa taaatactg tttcgtttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY