| General information | |||
| TC033158
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10291287..10306952 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033158 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05630 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC033158 RA
MSTGKRLAK RSIIGTRVC APGEDGKYY SGVIQAVKT PANFLENNN CINLTPNTR YTVRFDSRQ GNLGRTTAE FFEMDLIGP GFQSVLGLK LVPEQKCFV TYNGREVSG EVCKHYFER DEVIIRIHP PGSEAPFEV QKRLEEVRL LESRKSARL ADQDTDFAR LADMAGDRK RTASHTIDV PAPSTHLGS RKRRPSSSQ DDNYKDTQM DECSAALVL MSLSCSPHS PNFNGFPWR LSTSPDSNI SSSSASYRS TPSPPPRAY SPQSAPLSS SAGSDEGIV MDYQDEQPR KKKNVTRIV FQCMWPSCL KIRDTCEAI LAHLKDHDV LPGEEEFYF TEVELGLTS PPPTLSHRD MARPPHEDP DYQRQIVGS FRQTLLASA PPQSPHKLL KLSSPRPHS PKTPASPRR VRGENKKCR KVYGMDHRE QWCTQCKWK KACSRFGD
- >TC033158 RA
atgtcgacc ggaaagcga ttagcgaaa cgttccatc atcgggacg cgagtgtgc gctccggga gaggacggc aagtactac tccggagtg atccaggcg gtcaaaacc ccagccaac ttccttgaa aacaacaac tgcatcaac ctcaccccc aacaccaga tacaccgtg cgtttcgac tcccgacag ggcaacttg ggccgcacc actgccgag ttcttcgaa atggacctc atcggcccc ggattccag tccgtgctc gggctgaaa ctcgtcccg gagcagaaa tgtttcgtc acctacaac ggcagggag gtttccggt gaggtctgc aagcattat ttcgagcgc gacgaagtc atcatcagg atacatccc cccggatcc gaagcgcct ttcgaagtt caaaaacgc ctcgaagag gtcagactt ttggagtcg cggaagtcg gccaggctg gccgatcag gacacggac ttcgccagg ttggcggac atggcaggg gacaggaag aggacggcg tcgcacact attgacgtc ccggcgcct tccacgcat ctcggatcc cgcaaacgg cgtcccagc agcagccag gacgacaat tacaaagac actcaaatg gacgaatgc agcgccgct ttggtccta atgagtctc tcgtgttcg ccacactcg ccaaacttc aacggcttt ccatggcgg ctgagcacc tcgccggac agcaacatc agcagcagc tcggcctcc tatcggtcg actccctcg ccgccgccc agggcctac tccccgcag tcggccccc ttgtcgtcc agcgccggc tccgatgag ggcatcgtc atggactac caggacgaa cagcccagg aagaaaaag aacgtcacc cgaattgtg ttccaatgc atgtggcct agctgccta aaaatacga gacacctgt gaagccatc ctcgcccat ctcaaggac cacgacgtg ctgcccggc gaggaggag ttctacttc acggaggtg gagctgggg ctcaccagc ccgccgccg acgctgtcc caccgcgac atggcgcgg cccccgcac gaggacccc gactaccag cggcagatc gtgggctcc ttccggcag acgctgctg gcgtcggcg cccccgcag tcgccccac aagctgctg aagctgtcg tcgccgcgc ccccacagc cccaagacg ccggcgtcg ccgcgccgc gtccggggc gagaacaag aagtgccgc aaggtctac ggcatggac caccgggag cagtggtgc acgcagtgc aagtggaag aaggcctgc tccaggttc ggggactga
- >TC033158 RA
cgggttagc caatcggcg tatgtaggt cacagcggt cgaccaatg gcaggggcg cgctccgtc acgtggttt tgttgttat catcagctg tggccgggc cgctatgtc cgtccggag ccggagcca ctcctcccg gatcggccg cgcttctca ctccgggcg gttggagcg agacagcgc gatcgacgt cgagctttc gcgatcagc tgttgtttt acgctagcc ggccggcta cggcagtcg gcattcaac tgtttacag tgacgctgc ctagaccgc accgtttta ttatgagta tgtgcatat actgcgatt ttccagtga ttgaacgca acgtttctg tcgaatgtt ttgactatc atcaaggtt ctgtgcaac ggttgctcg tcaggaggc tgtacttca atgacgtgt gagaccccg gccggactt tgagcgatt tatcgagtg acggtactg aagatgtcg accggaaag cgattagcg aaacgttcc atcatcggg acgcgagtg tgcgctccg ggagaggac ggcaagtac tactccgga gtgatccag gcggtcaaa accccagcc aacttcctt gaaaacaac aactgcatc aacctcacc cccaacacc agatacacc gtgcgtttc gactcccga cagggcaac ttgggccgc accactgcc gagttcttc gaaatggac ctcatcggc cccggattc cagtccgtg ctcgggctg aaactcgtc ccggagcag aaatgtttc gtcacctac aacggcagg gaggtttcc ggtgaggtc tgcaagcat tatttcgag cgcgacgaa gtcatcatc aggatacat ccccccgga tccgaagcg cctttcgaa gttcaaaaa cgcctcgaa gaggtcaga cttttggag tcgcggaag tcggccagg ctggccgat caggacacg gacttcgcc aggttggcg gacatggca ggggacagg aagaggacg gcgtcgcac actattgac gtcccggcg ccttccacg catctcgga tcccgcaaa cggcgtccc agcagcagc caggacgac aattacaaa gacactcaa atggacgaa tgcagcgcc gctttggtc ctaatgagt ctctcgtgt tcgccacac tcgccaaac ttcaacggc tttccatgg cggctgagc acctcgccg gacagcaac atcagcagc agctcggcc tcctatcgg tcgactccc tcgccgccg cccagggcc tactccccg cagtcggcc cccttgtcg tccagcgcc ggctccgat gagggcatc gtcatggac taccaggac gaacagccc aggaagaaa aagaacgtc acccgaatt gtgttccaa tgcatgtgg cctagctgc ctaaaaata cgagacacc tgtgaagcc atcctcgcc catctcaag gaccacgac gtgctgccc ggcgaggag gagttctac ttcacggag gtggagctg gggctcacc agcccgccg ccgacgctg tcccaccgc gacatggcg cggcccccg cacgaggac cccgactac cagcggcag atcgtgggc tccttccgg cagacgctg ctggcgtcg gcgcccccg cagtcgccc cacaagctg ctgaagctg tcgtcgccg cgcccccac agccccaag acgccggcg tcgccgcgc cgcgtccgg ggcgagaac aagaagtgc cgcaaggtc tacggcatg gaccaccgg gagcagtgg tgcacgcag tgcaagtgg aagaaggcc tgctccagg ttcggggac tgatccaag tgacagtga cctcggcgc cgatctcag actagagtc aagtacaag gacggggac gttgatgtc gtgtttgat cgtttgatg gacgcgtga tcagtgtga agatctcag ccaaagtgc accacgtcg cgctacctt accgtaccg gacatttcg atgatgatt cgatcgctt tcgctgttt tggtattag agattaggt gagattgtg agattgatc atacctgcc atagtttgt aggggctat gagtatttt aaattatta taggaagta cacgccgtc cccgaatca atgcccgga cgacacttc ggggacggc ccgtatgta aagcgtcac gaaagacgc agcgagtca ctgccctgt ttattgctt ctgtgtact ttgtattat tgtcgatct ttttaaatt tttatctat tgtatattg ttaagaggc aatcgtgca cttgtatca aattcgtat gaaagaatc gtagttatt tacacattt cattacctc taaagtctt tcattttta ttattatta tttttgtta ttcctcata tttacattt atagagtat ttatataca taatataat tatattttt cagagtatt catatattt ttaatgtta agttgagat cattatcat gttaaattt taacacatt aaatagctc gtaaggtac ataacttat acattaaag aaaaataca ttatattta cattagtta caaacgtat atttagaat tcatccaga gaatgattt atgaatgaa cctgaaatt cgtcaaatt tattaataa agcgtatta tattat
