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Gene information
General information
TC033158
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014425,TC014427 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10291287..10306952
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033158
    iBeetle screen iB_05630

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033158 RA
      MSTGKRLAK RSIIGTRVC APGEDGKYY SGVIQAVKT 
PANFLENNN CINLTPNTR YTVRFDSRQ GNLGRTTAE 
FFEMDLIGP GFQSVLGLK LVPEQKCFV TYNGREVSG 
EVCKHYFER DEVIIRIHP PGSEAPFEV QKRLEEVRL 
LESRKSARL ADQDTDFAR LADMAGDRK RTASHTIDV 
PAPSTHLGS RKRRPSSSQ DDNYKDTQM DECSAALVL 
MSLSCSPHS PNFNGFPWR LSTSPDSNI SSSSASYRS 
TPSPPPRAY SPQSAPLSS SAGSDEGIV MDYQDEQPR 
KKKNVTRIV FQCMWPSCL KIRDTCEAI LAHLKDHDV 
LPGEEEFYF TEVELGLTS PPPTLSHRD MARPPHEDP 
DYQRQIVGS FRQTLLASA PPQSPHKLL KLSSPRPHS 
PKTPASPRR VRGENKKCR KVYGMDHRE QWCTQCKWK 
KACSRFGD
    CDS sequence
    • >TC033158 RA
      atgtcgacc ggaaagcga ttagcgaaa cgttccatc 
atcgggacg cgagtgtgc gctccggga gaggacggc 
aagtactac tccggagtg atccaggcg gtcaaaacc 
ccagccaac ttccttgaa aacaacaac tgcatcaac 
ctcaccccc aacaccaga tacaccgtg cgtttcgac 
tcccgacag ggcaacttg ggccgcacc actgccgag 
ttcttcgaa atggacctc atcggcccc ggattccag 
tccgtgctc gggctgaaa ctcgtcccg gagcagaaa 
tgtttcgtc acctacaac ggcagggag gtttccggt 
gaggtctgc aagcattat ttcgagcgc gacgaagtc 
atcatcagg atacatccc cccggatcc gaagcgcct 
ttcgaagtt caaaaacgc ctcgaagag gtcagactt 
ttggagtcg cggaagtcg gccaggctg gccgatcag 
gacacggac ttcgccagg ttggcggac atggcaggg 
gacaggaag aggacggcg tcgcacact attgacgtc 
ccggcgcct tccacgcat ctcggatcc cgcaaacgg 
cgtcccagc agcagccag gacgacaat tacaaagac 
actcaaatg gacgaatgc agcgccgct ttggtccta 
atgagtctc tcgtgttcg ccacactcg ccaaacttc 
aacggcttt ccatggcgg ctgagcacc tcgccggac 
agcaacatc agcagcagc tcggcctcc tatcggtcg 
actccctcg ccgccgccc agggcctac tccccgcag 
tcggccccc ttgtcgtcc agcgccggc tccgatgag 
ggcatcgtc atggactac caggacgaa cagcccagg 
aagaaaaag aacgtcacc cgaattgtg ttccaatgc 
atgtggcct agctgccta aaaatacga gacacctgt 
gaagccatc ctcgcccat ctcaaggac cacgacgtg 
ctgcccggc gaggaggag ttctacttc acggaggtg 
gagctgggg ctcaccagc ccgccgccg acgctgtcc 
caccgcgac atggcgcgg cccccgcac gaggacccc 
gactaccag cggcagatc gtgggctcc ttccggcag 
acgctgctg gcgtcggcg cccccgcag tcgccccac 
aagctgctg aagctgtcg tcgccgcgc ccccacagc 
cccaagacg ccggcgtcg ccgcgccgc gtccggggc 
gagaacaag aagtgccgc aaggtctac ggcatggac 
caccgggag cagtggtgc acgcagtgc aagtggaag 
aaggcctgc tccaggttc ggggactga
    mRNA sequence
    • >TC033158 RA
      cgggttagc caatcggcg tatgtaggt cacagcggt 
cgaccaatg gcaggggcg cgctccgtc acgtggttt 
tgttgttat catcagctg tggccgggc cgctatgtc 
cgtccggag ccggagcca ctcctcccg gatcggccg 
cgcttctca ctccgggcg gttggagcg agacagcgc 
gatcgacgt cgagctttc gcgatcagc tgttgtttt 
acgctagcc ggccggcta cggcagtcg gcattcaac 
tgtttacag tgacgctgc ctagaccgc accgtttta 
ttatgagta tgtgcatat actgcgatt ttccagtga 
ttgaacgca acgtttctg tcgaatgtt ttgactatc 
atcaaggtt ctgtgcaac ggttgctcg tcaggaggc 
tgtacttca atgacgtgt gagaccccg gccggactt 
tgagcgatt tatcgagtg acggtactg aagatgtcg 
accggaaag cgattagcg aaacgttcc atcatcggg 
acgcgagtg tgcgctccg ggagaggac ggcaagtac 
tactccgga gtgatccag gcggtcaaa accccagcc 
aacttcctt gaaaacaac aactgcatc aacctcacc 
cccaacacc agatacacc gtgcgtttc gactcccga 
cagggcaac ttgggccgc accactgcc gagttcttc 
gaaatggac ctcatcggc cccggattc cagtccgtg 
ctcgggctg aaactcgtc ccggagcag aaatgtttc 
gtcacctac aacggcagg gaggtttcc ggtgaggtc 
tgcaagcat tatttcgag cgcgacgaa gtcatcatc 
aggatacat ccccccgga tccgaagcg cctttcgaa 
gttcaaaaa cgcctcgaa gaggtcaga cttttggag 
tcgcggaag tcggccagg ctggccgat caggacacg 
gacttcgcc aggttggcg gacatggca ggggacagg 
aagaggacg gcgtcgcac actattgac gtcccggcg 
ccttccacg catctcgga tcccgcaaa cggcgtccc 
agcagcagc caggacgac aattacaaa gacactcaa 
atggacgaa tgcagcgcc gctttggtc ctaatgagt 
ctctcgtgt tcgccacac tcgccaaac ttcaacggc 
tttccatgg cggctgagc acctcgccg gacagcaac 
atcagcagc agctcggcc tcctatcgg tcgactccc 
tcgccgccg cccagggcc tactccccg cagtcggcc 
cccttgtcg tccagcgcc ggctccgat gagggcatc 
gtcatggac taccaggac gaacagccc aggaagaaa 
aagaacgtc acccgaatt gtgttccaa tgcatgtgg 
cctagctgc ctaaaaata cgagacacc tgtgaagcc 
atcctcgcc catctcaag gaccacgac gtgctgccc 
ggcgaggag gagttctac ttcacggag gtggagctg 
gggctcacc agcccgccg ccgacgctg tcccaccgc 
gacatggcg cggcccccg cacgaggac cccgactac 
cagcggcag atcgtgggc tccttccgg cagacgctg 
ctggcgtcg gcgcccccg cagtcgccc cacaagctg 
ctgaagctg tcgtcgccg cgcccccac agccccaag 
acgccggcg tcgccgcgc cgcgtccgg ggcgagaac 
aagaagtgc cgcaaggtc tacggcatg gaccaccgg 
gagcagtgg tgcacgcag tgcaagtgg aagaaggcc 
tgctccagg ttcggggac tgatccaag tgacagtga 
cctcggcgc cgatctcag actagagtc aagtacaag 
gacggggac gttgatgtc gtgtttgat cgtttgatg 
gacgcgtga tcagtgtga agatctcag ccaaagtgc 
accacgtcg cgctacctt accgtaccg gacatttcg 
atgatgatt cgatcgctt tcgctgttt tggtattag 
agattaggt gagattgtg agattgatc atacctgcc 
atagtttgt aggggctat gagtatttt aaattatta 
taggaagta cacgccgtc cccgaatca atgcccgga 
cgacacttc ggggacggc ccgtatgta aagcgtcac 
gaaagacgc agcgagtca ctgccctgt ttattgctt 
ctgtgtact ttgtattat tgtcgatct ttttaaatt 
tttatctat tgtatattg ttaagaggc aatcgtgca 
cttgtatca aattcgtat gaaagaatc gtagttatt 
tacacattt cattacctc taaagtctt tcattttta 
ttattatta tttttgtta ttcctcata tttacattt 
atagagtat ttatataca taatataat tatattttt 
cagagtatt catatattt ttaatgtta agttgagat 
cattatcat gttaaattt taacacatt aaatagctc 
gtaaggtac ataacttat acattaaag aaaaataca 
ttatattta cattagtta caaacgtat atttagaat 
tcatccaga gaatgattt atgaatgaa cctgaaatt 
cgtcaaatt tattaataa agcgtatta tattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY