Status
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Gene information
General information
TC033158
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014425,TC014427
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10291287..10306952
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033158
    iBeetle screen iB_05630

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033158 RA
      MSTGKRLAK RSIIGTRVC APGEDGKYY SGVIQAVKT 
      PANFLENNN CINLTPNTR YTVRFDSRQ GNLGRTTAE 
      FFEMDLIGP GFQSVLGLK LVPEQKCFV TYNGREVSG 
      EVCKHYFER DEVIIRIHP PGSEAPFEV QKRLEEVRL 
      LESRKSARL ADQDTDFAR LADMAGDRK RTASHTIDV 
      PAPSTHLGS RKRRPSSSQ DDNYKDTQM DECSAALVL 
      MSLSCSPHS PNFNGFPWR LSTSPDSNI SSSSASYRS 
      TPSPPPRAY SPQSAPLSS SAGSDEGIV MDYQDEQPR 
      KKKNVTRIV FQCMWPSCL KIRDTCEAI LAHLKDHDV 
      LPGEEEFYF TEVELGLTS PPPTLSHRD MARPPHEDP 
      DYQRQIVGS FRQTLLASA PPQSPHKLL KLSSPRPHS 
      PKTPASPRR VRGENKKCR KVYGMDHRE QWCTQCKWK 
      KACSRFGD
    CDS sequence
    • >TC033158 RA
      atgtcgacc ggaaagcga ttagcgaaa cgttccatc 
      atcgggacg cgagtgtgc gctccggga gaggacggc 
      aagtactac tccggagtg atccaggcg gtcaaaacc 
      ccagccaac ttccttgaa aacaacaac tgcatcaac 
      ctcaccccc aacaccaga tacaccgtg cgtttcgac 
      tcccgacag ggcaacttg ggccgcacc actgccgag 
      ttcttcgaa atggacctc atcggcccc ggattccag 
      tccgtgctc gggctgaaa ctcgtcccg gagcagaaa 
      tgtttcgtc acctacaac ggcagggag gtttccggt 
      gaggtctgc aagcattat ttcgagcgc gacgaagtc 
      atcatcagg atacatccc cccggatcc gaagcgcct 
      ttcgaagtt caaaaacgc ctcgaagag gtcagactt 
      ttggagtcg cggaagtcg gccaggctg gccgatcag 
      gacacggac ttcgccagg ttggcggac atggcaggg 
      gacaggaag aggacggcg tcgcacact attgacgtc 
      ccggcgcct tccacgcat ctcggatcc cgcaaacgg 
      cgtcccagc agcagccag gacgacaat tacaaagac 
      actcaaatg gacgaatgc agcgccgct ttggtccta 
      atgagtctc tcgtgttcg ccacactcg ccaaacttc 
      aacggcttt ccatggcgg ctgagcacc tcgccggac 
      agcaacatc agcagcagc tcggcctcc tatcggtcg 
      actccctcg ccgccgccc agggcctac tccccgcag 
      tcggccccc ttgtcgtcc agcgccggc tccgatgag 
      ggcatcgtc atggactac caggacgaa cagcccagg 
      aagaaaaag aacgtcacc cgaattgtg ttccaatgc 
      atgtggcct agctgccta aaaatacga gacacctgt 
      gaagccatc ctcgcccat ctcaaggac cacgacgtg 
      ctgcccggc gaggaggag ttctacttc acggaggtg 
      gagctgggg ctcaccagc ccgccgccg acgctgtcc 
      caccgcgac atggcgcgg cccccgcac gaggacccc 
      gactaccag cggcagatc gtgggctcc ttccggcag 
      acgctgctg gcgtcggcg cccccgcag tcgccccac 
      aagctgctg aagctgtcg tcgccgcgc ccccacagc 
      cccaagacg ccggcgtcg ccgcgccgc gtccggggc 
      gagaacaag aagtgccgc aaggtctac ggcatggac 
      caccgggag cagtggtgc acgcagtgc aagtggaag 
      aaggcctgc tccaggttc ggggactga
    mRNA sequence
    • >TC033158 RA
      cgggttagc caatcggcg tatgtaggt cacagcggt 
      cgaccaatg gcaggggcg cgctccgtc acgtggttt 
      tgttgttat catcagctg tggccgggc cgctatgtc 
      cgtccggag ccggagcca ctcctcccg gatcggccg 
      cgcttctca ctccgggcg gttggagcg agacagcgc 
      gatcgacgt cgagctttc gcgatcagc tgttgtttt 
      acgctagcc ggccggcta cggcagtcg gcattcaac 
      tgtttacag tgacgctgc ctagaccgc accgtttta 
      ttatgagta tgtgcatat actgcgatt ttccagtga 
      ttgaacgca acgtttctg tcgaatgtt ttgactatc 
      atcaaggtt ctgtgcaac ggttgctcg tcaggaggc 
      tgtacttca atgacgtgt gagaccccg gccggactt 
      tgagcgatt tatcgagtg acggtactg aagatgtcg 
      accggaaag cgattagcg aaacgttcc atcatcggg 
      acgcgagtg tgcgctccg ggagaggac ggcaagtac 
      tactccgga gtgatccag gcggtcaaa accccagcc 
      aacttcctt gaaaacaac aactgcatc aacctcacc 
      cccaacacc agatacacc gtgcgtttc gactcccga 
      cagggcaac ttgggccgc accactgcc gagttcttc 
      gaaatggac ctcatcggc cccggattc cagtccgtg 
      ctcgggctg aaactcgtc ccggagcag aaatgtttc 
      gtcacctac aacggcagg gaggtttcc ggtgaggtc 
      tgcaagcat tatttcgag cgcgacgaa gtcatcatc 
      aggatacat ccccccgga tccgaagcg cctttcgaa 
      gttcaaaaa cgcctcgaa gaggtcaga cttttggag 
      tcgcggaag tcggccagg ctggccgat caggacacg 
      gacttcgcc aggttggcg gacatggca ggggacagg 
      aagaggacg gcgtcgcac actattgac gtcccggcg 
      ccttccacg catctcgga tcccgcaaa cggcgtccc 
      agcagcagc caggacgac aattacaaa gacactcaa 
      atggacgaa tgcagcgcc gctttggtc ctaatgagt 
      ctctcgtgt tcgccacac tcgccaaac ttcaacggc 
      tttccatgg cggctgagc acctcgccg gacagcaac 
      atcagcagc agctcggcc tcctatcgg tcgactccc 
      tcgccgccg cccagggcc tactccccg cagtcggcc 
      cccttgtcg tccagcgcc ggctccgat gagggcatc 
      gtcatggac taccaggac gaacagccc aggaagaaa 
      aagaacgtc acccgaatt gtgttccaa tgcatgtgg 
      cctagctgc ctaaaaata cgagacacc tgtgaagcc 
      atcctcgcc catctcaag gaccacgac gtgctgccc 
      ggcgaggag gagttctac ttcacggag gtggagctg 
      gggctcacc agcccgccg ccgacgctg tcccaccgc 
      gacatggcg cggcccccg cacgaggac cccgactac 
      cagcggcag atcgtgggc tccttccgg cagacgctg 
      ctggcgtcg gcgcccccg cagtcgccc cacaagctg 
      ctgaagctg tcgtcgccg cgcccccac agccccaag 
      acgccggcg tcgccgcgc cgcgtccgg ggcgagaac 
      aagaagtgc cgcaaggtc tacggcatg gaccaccgg 
      gagcagtgg tgcacgcag tgcaagtgg aagaaggcc 
      tgctccagg ttcggggac tgatccaag tgacagtga 
      cctcggcgc cgatctcag actagagtc aagtacaag 
      gacggggac gttgatgtc gtgtttgat cgtttgatg 
      gacgcgtga tcagtgtga agatctcag ccaaagtgc 
      accacgtcg cgctacctt accgtaccg gacatttcg 
      atgatgatt cgatcgctt tcgctgttt tggtattag 
      agattaggt gagattgtg agattgatc atacctgcc 
      atagtttgt aggggctat gagtatttt aaattatta 
      taggaagta cacgccgtc cccgaatca atgcccgga 
      cgacacttc ggggacggc ccgtatgta aagcgtcac 
      gaaagacgc agcgagtca ctgccctgt ttattgctt 
      ctgtgtact ttgtattat tgtcgatct ttttaaatt 
      tttatctat tgtatattg ttaagaggc aatcgtgca 
      cttgtatca aattcgtat gaaagaatc gtagttatt 
      tacacattt cattacctc taaagtctt tcattttta 
      ttattatta tttttgtta ttcctcata tttacattt 
      atagagtat ttatataca taatataat tatattttt 
      cagagtatt catatattt ttaatgtta agttgagat 
      cattatcat gttaaattt taacacatt aaatagctc 
      gtaaggtac ataacttat acattaaag aaaaataca 
      ttatattta cattagtta caaacgtat atttagaat 
      tcatccaga gaatgattt atgaatgaa cctgaaatt 
      cgtcaaatt tattaataa agcgtatta tattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY